Lecsl2-2 gene for improving flavor of lentinula edodes and application thereof
CN119842760BActive Publication Date: 2026-08-28GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
Patent Information
- Application Number
- CN202510008814.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-03
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2045-01-03
AI Technical Summary
Technical Problem
[0004]现有技术中，我们通常通过选择野生香菇与商品香菇杂交的方法来提高商品香菇的香味，但是，我们在实际杂交工作中发现：由于杂交的随机性，我们往往需要培育到子实体时期才能检测出香菇的香气，育种进程很慢，而且对杂交后代的大量筛选工作也进一步加大了我们的工作量
Benefits of technology
[0019]1、本发明以申请人自行筛选驯化的香气浓郁的野生品种：融香7号和商品香菇L808的香气基因：Leggt1、Leggt5、Lecsl2和Lecsl3进行表达分析，发现：香气浓郁的融香7号品种其香味基因Lecsl2的表达量显著高于商品香菇L808，基于这个结论进行深入分析发现在Lecsl2基因的等位基因中，仅有融香7号含有Lecsl2-2基因，由此推测，含有该基因的杂交后代会表现出含有更高的香菇素，经过对含该基因的单倍体菌株进行分离后，我们进行单双杂交，并对杂交后代进行香菇素测定，最终发现：含有Lecsl2-2基因的杂交后代表现为含有更高的香菇素。由此，我们可以通过改进育种方法：即对香菇的单倍体菌株进行PCR扩增鉴定，定向选择含有Lecsl2-2基因的单倍体菌株进行杂交，从而能选育出香菇素含量更高的杂交后代，为下一步提高商品香菇的香气提供了新的育种思路，有效缩短了育种时间。
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Abstract
The present application relates to edible mushroom gene breeding technology field, especially to the Lecsl2-2 gene for improving the aroma of lentinus edodes and its application, the present application expresses and analyzes the aroma gene of Rongxiang No. 7 and commercial lentinus edodes L808, and finds that the expression amount of aroma gene Lecsl2 of Rongxiang No. 7 with rich aroma is significantly higher than that of commercial lentinus edodes L808. In-depth analysis finds that only Rongxiang No. 7 contains higher expression Lecsl2-2 gene, and the hybrid offspring containing Lecsl2-2 gene shows higher lentinan. By improving the breeding method, i.e. PCR amplification identification of lentinus edodes haploid strain, directional selection of haploid strain containing Lecsl2-2 gene for hybridization, lentinus edodes hybrid offspring with higher lentinan content can be selected, which provides a new breeding idea for improving the aroma of commercial lentinus edodes in the next step, and effectively shortens the breeding time.
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Citation Information
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