Sputum homogenizing, shaking-up and sampling integrated pretreatment equipment and use method

Abstract

The invention discloses sputum homogenizing, shaking up and sampling integrated pretreatment equipment and a use method. The equipment comprises a main base body, a digestive juice filling unit, a sample sucking unit, a tube group clamping control unit, an internal and external conveying unit, a shaking unit which is arranged between the tube group clamping control unit and the internal and external conveying unit and is used for controlling the tube group clamping control unit to swing back and forth, and a sputum cup tube frame which is arranged on the tube group clamping control unit, the sputum cups are mounted on the sputum cup pipe frame in batches; the digestive juice filling unit and the sample suction unit are symmetrically arranged relative to the center of the main base body and have the same structure; through integration of multiple operations of sputum cup cover opening and closing, digestive juice filling, uniform shaking and standing and sample extraction, the pretreatment requirement of a sputum nucleic acid detection method is met, the sputum treatment efficiency and safety are greatly improved, and the problems that an existing pretreatment automation technology can only realize a test paper detection method, and the detection efficiency is low are solved. And the measurement accuracy is limited.

Description

Technical Field

[0001] The present invention belongs to the technical field of sputum pretreatment equipment, and more specifically, relates to a sputum homogenization, shaking, sampling integrated pretreatment equipment and a use method. Background Art

[0002] At present, sputum samples are mainly used for sputum cytology and respiratory pathogen detection in clinical practice. The existing detection method is that the patient spits sputum directly into the collection container, and then the tester opens the lid and adds dilution digestion fluid to the collection container to dilute the sputum into a homogenized test sample, then closes the lid and shakes it, and then opens the lid to absorb the sample. At present, sputum digestion mainly relies on manual operation, which is easy to cause the spread of respiratory diseases and mutual contamination of sputum samples, and the operation is cumbersome. Therefore, it is of great significance to propose a device or method for efficiently processing sputum for subsequent detection to improve work efficiency and staff safety.

[0003] In order to solve the above technical problems, Chinese invention patent CN112629940A discloses a sputum sampling detection device and its use method, the sputum sampling detection device includes a detection tube, a collection tube and a test paper; the detection tube is provided with a accommodating chamber, a sealing film and a test paper chamber; the sealing film divides the accommodating chamber into a collection tube accommodating portion and a diluent accommodating portion; the test paper chamber is located at the front side of the accommodating chamber, and a connecting channel is provided between the collection tube accommodating portion and the test paper chamber; the connecting channel is located above the sealing film; the diluent accommodating portion is filled with sputum diluent; the collection tube is detachably connected to the collection tube accommodating portion, and a membrane-penetrating structure is provided at the lower end of the collection tube, and the membrane-penetrating structure is used to pierce the sealing film; the test paper is arranged in the test paper chamber. The technical solution of the present application adopts a two-stage pressing design. After the sealing film is pierced, the sampling rod needs to be further pressed to allow the diluent to enter the test paper chamber, thereby effectively preventing the problem of misoperation.

[0004] The above patent scheme adopts a double-tube design to achieve an integrated processing effect of sampling, dilution and detection, but there are still the following technical problems: (1) After diluting the sputum, the scheme directly enters the test paper detection link, and the two processes are not fully shaken and left to stand, resulting in poor accuracy of the test results; (2) After vortex oscillation and standing, the sample is sucked into the nucleic acid extraction plate by the sample gun, and the nucleic acid detection method is adopted. Compared with the test paper method, the nucleic acid detection method of sputum is more accurate, but the above scheme cannot be transferred to the nucleic acid detection method. (3) Based on the standard operating procedures of the sputum nucleic acid detection method, a set of sputum pretreatment devices with integrated sputum homogenization, shaking and sampling should be designed to improve the overall operation efficiency. Summary of the invention

[0005] In response to the above defects or improvement needs of the prior art, the present invention provides an integrated pre-treatment device for sputum homogenization, shaking and sampling and a method for use. By integrating the opening and closing of the sputum cup cover, filling of digestive fluid, shaking and standing, and sample extraction, the pre-treatment requirements of the sputum nucleic acid detection method are met, the sputum treatment efficiency and safety are greatly improved, and the technical problem that the existing pre-treatment automation technology can only realize the test paper detection method, resulting in limited measurement accuracy, is solved.

[0006] In order to solve the above technical problems, according to the first aspect of the present invention, a sputum homogenization, shaking, sampling integrated pre-treatment device comprises:

[0007] A main base, a digestive fluid filling unit and a sample suction unit respectively located at the upper left corner and the upper right corner of the main base, a tube group clamping control unit arranged in the central area of ​​the main base, an internal and external transmission unit vertically arranged in the central area and used to drive the tube group clamping control unit to move up and down, a shaking unit arranged between the tube group clamping control unit and the internal and external transmission unit and used to control the reciprocating swing of the tube group clamping control unit, a sputum cup rack installed on the tube group clamping control unit, and sputum cups installed in batches on the sputum cup rack; the digestive fluid filling unit and the sample suction unit are symmetrically arranged about the center of the main base and have the same structure;

[0008] The tube set clamping control unit comprises a main housing, a clamping control component arranged in the clamping functional area of ​​the main housing for clamping and opening and closing the sputum cup, and a tube set installation area for installing the sputum cup tube set in the tube set clamping control unit;

[0009] The sputum cup tube rack comprises a placement assembly connected in a single row array; the placement assembly comprises a square frame for mounting the sputum cup, a side handle arranged on the side of the square frame, side windows opened on the left and right sides of the square frame, and a handle body installation opening that penetrates and is opened on the side handle;

[0010] The sputum cup comprises a main cup body, a cup mouth slide groove arranged at the opening of the main cup body, a sliding cover horizontally slidably connected to the sputum cup, and an operating handle vertically arranged on the lower surface of the outer side of the sliding cover.

[0011] Preferably, the digestive fluid filling unit comprises:

[0012] An operating position control component disposed obliquely downward at the main body filling function area, an operating quantity control component disposed at the output end of the operating position control component, and an operating gun installed on the operating position control component and the operating quantity control component;

[0013] The operating position control assembly includes a first driving motor fixedly connected to the main base, a first driving screw coaxially fixedly connected to the first driving motor, a first nut block threadedly connected to the first driving screw, a carrier fixed on the first nut block, and a clamping part provided on the upper surface of the carrier for clamping the operating gun;

[0014] The operating gun comprises a main body clamped by a clamping part, a push handle clamped by the operating amount control component and realizing push amount control, and a gun head whose front end position is controlled by the operating position control component.

[0015] Preferably, the operation amount control component includes:

[0016] A second drive motor fixed to the rear side of the carrier, a second drive screw rod coaxially fixedly connected to the second drive motor, a second nut block maintaining threaded linear transmission with the second drive screw rod, and a bayonet for clamping the push handle on the upper surface of the second nut block.

[0017] Preferably, the clamping control assembly comprises:

[0018] A clamping control slide fixed to the bottom surface of the clamping functional area of ​​the main shell, a clamping control drive motor fixed to the left side of the clamping control slide, a clamping control transmission screw rod coaxially fixedly connected to the clamping control drive motor, a clamping control transmission block arranged on the clamping control transmission screw rod, a first base body fixedly connected to the clamping control transmission block, a second base body slidably connected to the horizontal mounting hole in the first base body, a forward push spring arranged between the first base body and the second base body, a cup body clamping plate fixed to the right end of the second base body for clamping a batch of sputum cups placed in the tube group mounting area, a non-slip rubber pad layer attached to the front side of the cup body clamping plate, a cup handle clamping assembly arranged on the second base body and used for clamping the sputum cup operating handle, a cup cap opening and closing control assembly for controlling the cup handle clamping assembly to slide horizontally, and a cup handle disengaging assembly for releasing the cup handle clamping assembly from the clamping state of the operating handle.

[0019] Preferably, the cup handle clamping assembly comprises:

[0020] An initial clamping groove opened on the upper surface of the second base and located on the left side of the cup body clamping plate, a clamping slider placed in the initial clamping groove in the initial state, a side lifting angle protruding from the front and rear sides of the clamping slider, a horizontal sliding surface connected to the lower surface of the side lifting angle and slidable along the upper surface of the first base, a cup handle clamping groove provided on the upper surface of the clamping slider for inserting the operating handle, a rubber layer provided on the side of the cup handle clamping groove, and a push-up angle which is consistent with the height of the side lifting angle in the initial state of the clamping slider and is horizontally provided on the right side of the first base.

[0021] Preferably, the cup handle card removal assembly comprises:

[0022] A slot body vertically opened on the bottom surface of the initial slot, a pullback spring fixedly connected to the bottom surface of the slot body, and a pullback rope fixedly connected to the upper end of the pullback spring; the upper end of the pullback rope is fixedly connected to the clamping slider.

[0023] Preferably, the cup cap opening and closing control assembly comprises:

[0024] A magnet fixed on the clamping slider, a transition slot opened on the left surface of the initial clamping slot, a main slot maintained at the same height as the transition slot and opened on the upper surface of the first substrate, and a magnetic suction device fixed on the left side of the main slot for controlling attraction or discharge to the magnet.

[0025] Preferably, the shaking unit comprises:

[0026] A shaking drive motor is fixedly arranged at the output end of the internal and external transmission unit, and a connecting shaft is coaxially fixedly connected to the output end of the shaking drive motor, and the connecting shaft is fixedly connected to the side of the pipe group clamping control unit.

[0027] Preferably, the internal and external transmission unit comprises:

[0028] A transmission drive motor is arranged at the center of the bottom of the main base, a vertical transmission screw rod is coaxially fixedly connected to the output shaft of the transmission drive motor, and a transmission nut block is threadedly connected to the vertical transmission screw rod; the shaking drive motor is fixedly connected to the transmission nut block.

[0029] According to a second aspect of the present invention, a method for using a sputum homogenization, shaking, and sampling integrated pre-treatment device comprises the following steps:

[0030] S100: First, start the transmission drive motor, move the tube group clamp control unit as a whole to the second pick-up window, install the sputum cups in batches into the sputum cup tube rack, keep the main cup body placed in the square frame, and place the operating handle in the handle body installation opening;

[0031] S200: Control the clamping control driving motor to rotate, thereby driving the first base to move horizontally to the right, and under the action of the forward push spring, the second base is pushed to move to the right, and then the rubber cushion layer contacts and presses the sputum cup main cup body;

[0032] S300: Continue to control the clamping control driving motor to rotate. At this time, the second base body does not move forward and keeps pressing the sputum cup. At the same time, the first base body continues to move to the right, so that the front push-up angle contacts the side lift-up angle and pushes the clamping slider upward, so that it finally disengages from the initial clamping groove, so that the operating handle above the clamping slider is clamped into the cup handle clamping groove, and the horizontal sliding surface of the clamping slider is flush with the upper surface of the first base body; then, the inner and outer conveying units are controlled to move in the opposite direction, so as to bring the tube group clamping control unit to the main operating area, ready to enter the pre-processing operation;

[0033] S400: The magnetic attraction device is powered on to attract the magnet, so that the clamping slide slides horizontally to the left along the transition slide groove and the main slide groove, and then the operating handle on the clamping slide is pulled to the left to open the sputum cup cover;

[0034] S500: by controlling the first driving motor in the digestive fluid filling unit, the front end of the operating gun is brought above the sputum cup, and by controlling the second driving motor in the digestive fluid filling unit, the amount of digestive fluid injected is controlled;

[0035] S600: The electromagnetic suction device is reversed to generate a repulsive force on the magnet, thereby pushing the card-joining slider to the right, thereby closing the sputum cup cover;

[0036] S700: By controlling the shaking drive motor, the tube group clamp control unit is shaken left and right, thereby mixing the sputum and digestive fluid in the sputum cup, and then standing for a fixed time;

[0037] S800: By controlling the forward electromagnetic suction device to generate attraction to the magnet, the sputum cup cover is opened, and the first drive motor and the second drive motor in the sample suction unit are started to achieve quantitative suction of the sample in the sputum cup; then, the sputum cup cover is controlled to be closed;

[0038] S900: The tube group clamping control unit is pushed out from the second access window through the internal and external conveying unit to facilitate the staff to take it out.

[0039] In general, the above technical solutions conceived by the present invention can achieve the following beneficial effects compared with the prior art:

[0040] 1. The sputum homogenization, shaking and sampling integrated pre-treatment equipment of the present invention meets the pre-treatment requirements of sputum nucleic acid detection method by integrating multiple operations such as opening and closing the sputum cup cover, filling digestive fluid, shaking and standing, and sample extraction, greatly improves the sputum treatment efficiency and safety, and solves the technical problem that the existing pre-treatment automation technology can only realize the test paper detection method, resulting in limited measurement accuracy.

[0041] 2. The integrated sputum homogenization, shaking and sampling pre-treatment equipment of the present invention realizes the clamping of the sputum cup body and the engagement of the cup handle through a single driving motor and ingenious use of the push angle structure. Combined with the electromagnetic device, the opening and closing of the sputum cup cap can be realized conveniently and quickly, which simplifies the difficulty of system control, reduces the cost of use, and is more convenient for the acceptance and promotion of subsequent products. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is a first use state diagram of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0043] Figure 2 This is a second use state diagram of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0044] Figure 3 This is a diagram of a third use state of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0045] Figure 4 This is a schematic diagram of the structure of a sputum cup tube stand of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0046] Figure 5 This is a schematic diagram of the cross-sectional structure of a sputum cup of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0047] Figure 6 This is a schematic diagram of the overall structure of a tube group clamp control unit of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0048] Figure 7 This is a schematic diagram of the side structure of a tube group clamp control unit of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0049] Figure 8 This is a schematic diagram of the structure of a clamping control component of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0050] Fig. 9 This is a schematic diagram of the main structure of a cup handle clamping assembly of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0051] Fig.10 This is a schematic diagram of the side view of the structure of a cup handle clamping assembly of a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention;

[0052] Fig.11 The present invention is a flowchart of a method for using a sputum homogenization, shaking and sampling integrated pre-treatment device according to an embodiment of the present invention.

[0053] In all the drawings, the same reference numerals represent the same technical features, specifically: 1-main body, 101-filling functional area, 102-sampling functional area, 103-main operation area, 104-first pick-up and drop window, 105-second pick-up and drop window, 106-third pick-up and drop window, 2-digestive fluid filling unit, 210-operating position control component, 211-first drive motor, 212-first drive screw, 213-first nut block, 214-carrying body, 215-clamping part, 220-operating amount control component, 221-second driving motor, 222-second driving screw rod, 223-second nut block, 230-operating gun, 231-main body, 232-push handle, 233-gun head, 3-sample suction unit, 4-tube group clamping control unit, 400-main housing, 401-clamping function area, 402-electric control management area, 403-tube group installation area, 410-clamping control assembly, 411-clamping control slide, 412-clamping control driving motor, 413-clamping control transmission screw rod, 414-clamping control transmission block, 415-first base, 416-second base, 417-cup handle clamping assembly, 4170-initial clamping slot, 4171-clamping slider, 4172-side lifting angle, 4173-horizontal sliding surface, 4174-cup handle clamping slot, 4175-rubber layer, 4176-lifting angle, 418-cup handle unclamping assembly, 4181-slot body, 4182-pullback spring, 4183-pullback rope, 419-cup cap opening and closing control assembly, 4191-transition slide, 4192-main slide, 4193-magnetic device, 4194-magnet, 4110-forward spring, 41 11-cup body clamping plate, 4112-rubber cushion, 5-inside and outside transmission unit, 501-transmission drive motor, 502-vertical transmission screw, 503-transmission nut block, 6-shaking unit, 601-shaking drive motor, 602-connecting shaft, 603-side positioning block, 7-sputum cup tube rack, 700-placement component, 701-square frame, 702-side handle, 703-side window, 704-handle body installation port, 8-sputum cup, 801-main cup body, 802-cup mouth slide groove, 803-slide cover, 804-operating handle. DETAILED DESCRIPTION

[0054] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0055] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0056] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0057] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0058] like Figures 1 to 10 As shown, in an embodiment of the present invention, the sputum homogenization, shaking, and sampling integrated pre-treatment device comprises:

[0059] A main matrix 1, a digestive solution filling unit 2 and a sample suction unit 3 respectively located at the upper left corner and the upper right corner of the main matrix 1, a tube group clamping control unit 4 arranged in the central area of ​​the main matrix 1, an internal and external transmission unit 5 vertically arranged in the central area and used to drive the tube group clamping control unit 4 to move up and down, a shaking unit 6 arranged between the tube group clamping control unit 4 and the internal and external transmission unit 5 and used to control the reciprocating swing of the tube group clamping control unit 4, a sputum cup tube rack 7 installed on the tube group clamping control unit 4, and sputum cups 8 installed in batches on the sputum cup tube rack 7; the digestive solution filling unit 2 and the sample suction unit 3 are symmetrically arranged about the center of the main matrix 1 and have the same structure;

[0060] The tube set clamping control unit 4 comprises a main housing 400, a clamping control component 410 disposed in the clamping functional area of ​​the main housing 400 for clamping and opening and closing the sputum cup, and a tube set installation area 403 for installing the sputum cup tube set on the tube set clamping control unit 4;

[0061] The sputum cup tube rack 7 includes a placement assembly 700 connected in a single row array; the placement assembly 700 includes a square frame 701 for mounting the sputum cup 8, a side handle 702 provided on the side of the square frame 701, side windows 703 opened on the left and right sides of the square frame 701, and a handle body installation opening 704 opened through the side handle 702;

[0062] The sputum cup 8 comprises a main cup body 801 , a cup mouth slide groove 802 provided at the opening of the main cup body 801 , a sliding cover 803 horizontally slidably connected along the cup mouth slide groove 802 , and an operating handle 804 vertically provided on the lower surface of the outer side of the sliding cover 803 .

[0063] like Figure 2 As shown, in this embodiment of the present invention, the digestive fluid filling unit 2 includes:

[0064] An operation position control component 210 disposed obliquely downward at the filling function area 101 of the main body 1, an operation amount control component 220 disposed at the output end of the operation position control component 210, and an operation gun 230 installed on the operation position control component 210 and the operation amount control component 220;

[0065] The operating position control assembly 210 includes a first driving motor 211 fixedly connected to the main base 1, a first driving screw 212 coaxially fixedly connected to the first driving motor 211, a first nut block 213 threadedly connected to the first driving screw 212, a carrier 214 fixed on the first nut block 213, and a clamping portion 215 provided on the upper surface of the carrier 214 for clamping the operating gun 230;

[0066] The operating gun 230 includes a main body 231 clamped by the clamping part 215 , a push handle 232 clamped by the operating amount control component 220 and realizing push amount control, and a gun head 233 whose front end position is controlled by the operating position control component 210 .

[0067] like Figure 2 As shown, in this embodiment of the present invention, the operation amount control component 220 includes:

[0068] A second driving motor 221 fixed to the rear side of the carrier 214, a second driving screw 222 coaxially and fixedly connected to the second driving motor 221, a second nut block 223 maintaining threaded linear transmission with the second driving screw 222, and a bayonet provided on the upper surface of the second nut block 223 for clamping the push handle 232.

[0069] like Figure 1 , Figure 6 and Figure 8 As shown, in this embodiment of the present invention, the clamping control assembly 410 includes:

[0070] A clamping control slide 411 fixed to the bottom surface of the clamping functional area 401 of the main housing 400, a clamping control driving motor 412 fixed to the left side of the clamping control slide 411, a clamping control transmission screw rod 413 coaxially fixedly connected to the clamping control driving motor 412, a clamping control transmission block 414 provided on the clamping control transmission screw rod 413, a first base 415 fixedly connected to the clamping control transmission block 414, a second base 416 slidably connected to the horizontal mounting hole in the first base 415, and a second base 416 provided between the first base 415 and the second base 416. A forward push spring 4110, a cup body clamping plate 4111 fixed to the right end of the second base 416 for clamping a batch of sputum cups placed in the tube group installation area 403, a non-slip rubber pad 4112 attached to the front side of the cup body clamping plate 4111, a cup handle clamping assembly 417 provided on the second base 416 and used for clamping the sputum cup operating handle 804, a cup cap opening and closing control assembly 419 for controlling the cup handle clamping assembly 417 to slide horizontally, and a cup handle disengaging assembly 418 for releasing the cup handle clamping assembly 417 from the clamping state of the operating handle 804.

[0071] like Figure 1 , Figure 6 and Figure 8 As shown, in this embodiment of the present invention, the cup handle clamping assembly 417 includes:

[0072] An initial card slot 4170 is opened on the upper surface of the second base 416 and is located on the left side of the cup body clamping plate 4111, a card-connecting slider 4171 is placed in the initial card slot 4170 in the initial state, a side lifting angle 4172 protruding from the front and rear sides of the card-connecting slider 4171, a horizontal sliding surface 4173 is connected to the lower surface of the side lifting angle 4172 and can slide along the upper surface of the first base 415, a cup handle card slot 4174 is provided on the upper surface of the card-connecting slider 4171 for inserting the operating handle 804, a rubber layer 4175 is provided on the side of the cup handle card slot 4174, and a push-up angle 4176 is consistent in height with the side lifting angle 4172 of the card-connecting slider 4171 in the initial state and is horizontally provided on the right side of the first base 415.

[0073] like Figure 1 , Figure 6 and Figure 8 As shown, in this embodiment of the present invention, the cup handle disengaging assembly 418 includes:

[0074] A groove body 4181 vertically opened on the bottom surface of the initial card groove 4170, a pull-back spring 4182 fixedly connected to the bottom surface of the groove body 4181, and a pull-back rope 4183 fixedly connected to the upper end of the pull-back spring 4182; the upper end of the pull-back rope 4183 is fixedly connected to the card-engaging slider 4171.

[0075] like Figure 1 , Figure 6 and Figure 8 As shown, in this embodiment of the present invention, the cup cap opening and closing control component 419 includes:

[0076] A magnet 4194 fixed on the locking slider 4171, a transition groove 4191 opened on the left surface of the notch of the initial locking groove 4170, a main groove 4192 maintained at the same height as the transition groove 4191 and opened on the upper surface of the first base 415, and a magnetic suction device 4193 fixed on the left side of the main groove 4192 for controlling the attraction or discharge to the magnet 4194.

[0077] like Figure 7 As shown, in this embodiment of the present invention, the shaking unit 6 includes:

[0078] A shaking drive motor 601 is fixedly arranged at the output end of the internal and external transmission unit 5, and a connecting shaft 602 is coaxially and fixedly connected to the output end of the shaking drive motor 601, and the connecting shaft 602 is fixedly connected to the side of the tube group clamping control unit 4.

[0079] like Figure 1 and Figure 7 As shown, in this embodiment of the present invention, the internal and external transmission unit 5 includes:

[0080] A transmission drive motor 501 is arranged at the bottom center of the main base 1, a vertical transmission screw 502 is coaxially and fixedly connected to the output shaft of the transmission drive motor 501, and a transmission nut block 503 is threadedly connected to the vertical transmission screw 502; the shaking drive motor 601 is fixedly connected to the transmission nut block 503.

[0081] Working principle of the present invention:

[0082] S100: First, start the transmission drive motor 501, move the tube group clamp control unit 4 as a whole to the second pick-up window 105, install the sputum cups 8 in batches into the sputum cup tube rack 7, keep the main cup body 801 placed in the square frame 701, and place the operating handle 804 in the handle body installation opening 704;

[0083] S200: Control the clamping control driving motor 412 to rotate, thereby driving the first base 415 to move horizontally to the right, and under the action of the forward push spring 4110, the second base 416 is pushed to move to the right, and then the rubber cushion layer 4112 contacts and presses the sputum cup main cup body 801;

[0084] S300: Continue to control the clamping control driving motor 412 to rotate. At this time, the second base 416 does not move forward and keeps pressing the sputum cup. At the same time, the first base 415 continues to move to the right, so that the front push angle 4176 contacts the side lifting angle 4172 and pushes the clamping slider 4171 upward, so that it finally disengages from the initial clamping groove 4170, so that the operating handle 804 above the clamping slider 4171 is clamped into the cup handle clamping groove 4174, and the horizontal sliding surface 4173 of the clamping slider 4171 is flush with the upper surface of the first base 415; then, control the internal and external conveying unit 5 to move in the opposite direction, so as to bring the tube group clamping control unit 4 to the main working area 103, ready to enter the pre-processing operation;

[0085] S400: The magnetic attraction device 4193 is energized to attract the magnet 4194, so that the clamping slide 4171 slides horizontally to the left along the transition slide groove 4191 and the main slide groove 4192, and then the operating handle 804 on the clamping slide 4171 is pulled to the left to open the sputum cup 8;

[0086] S500: by controlling the first driving motor 211 in the digestive liquid filling unit 2, the front end of the operating gun 230 is brought above the sputum cup, and by controlling the second driving motor 221 in the digestive liquid filling unit 2, the amount of digestive liquid injected is controlled;

[0087] S600: The electromagnetic suction device 4193 is turned on in the reverse direction to generate a repulsive force on the magnet 4194, thereby pushing the clamping slider 4171 to the right, thereby closing the sputum cup cover;

[0088] S700: by controlling the shaking driving motor 601, the tube group clamp control unit 4 is shaken left and right, thereby achieving a mixing operation of the sputum and digestive fluid in the sputum cup, and then standing for a fixed time;

[0089] S800: By controlling the forward electromagnetic suction device 4193 to generate attraction on the magnet 4194, the sputum cup cover is opened, and the first drive motor 211 and the second drive motor 221 in the sample suction unit 3 are started to achieve quantitative suction of the sample in the sputum cup; then, the sputum cup cover is controlled to be closed;

[0090] S900: The tube group clamping control unit 4 is pushed out from the second access window 105 through the internal and external conveying unit 5 to facilitate the staff to take it out.

[0091] In addition, based on the solution, the operating gun 230 can be taken out from the first access window 104 and the third access window 106 to achieve one-time replacement; at the same time, a negative pressure fan can be added in the device to extract the internal polluted air in real time to further ensure safety. The first access window, the second access window and the third access window are normally closed.

[0092] In the embodiment of the present invention, by integrating the opening and closing of the sputum cup cover, the filling of digestive fluid, the shaking and standing, and the sample extraction, the pre-treatment requirements of the sputum nucleic acid detection method are met, the sputum processing efficiency and safety are greatly improved, and the technical problem that the existing pre-treatment automation technology can only realize the test paper detection method, resulting in limited measurement accuracy, is solved.

[0093] In addition, for the tube group clamping control unit 4, through a single driving motor and clever use of the push angle structure, the sputum cup body clamping and cup handle engagement operations are realized. Combined with the electromagnetic device, the opening and closing operations of the sputum cup cap body are conveniently and quickly realized, which simplifies the difficulty of system control while reducing the cost of use, making it easier for subsequent products to be accepted and promoted.

[0094] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the present application. The above are only preferred implementations of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present application, and these improvements and variations should also be regarded as the protection scope of the present application.

Claims

1. A sputum homogenization, shaking, sampling integrated pre-treatment equipment, characterized in that: include: A main base (1), a digestive fluid filling unit (2) and a sample suction unit (3) respectively located at the upper left corner and the upper right corner of the main base (1), a tube group clamping control unit (4) arranged in the central area of ​​the main base (1), an internal and external transmission unit (5) vertically arranged in the central area and used to drive the tube group clamping control unit (4) to move up and down, a shaking unit (6) arranged between the tube group clamping control unit (4) and the internal and external transmission unit (5) and used to control the reciprocating swing of the tube group clamping control unit (4), a sputum cup tube rack (7) installed on the tube group clamping control unit (4), and sputum cups (8) installed in batches on the sputum cup tube rack (7); the digestive fluid filling unit (2) and the sample suction unit (3) are symmetrically arranged about the center of the main base (1) and have the same structure; The tube set clamping control unit (4) comprises a main shell (400), a clamping control component (410) arranged in a clamping functional area of ​​the main shell (400) for clamping and opening and closing a sputum cup, and a tube set installation area (403) for installing a sputum cup tube set on the tube set clamping control unit (4); The sputum cup tube rack (7) comprises a placement assembly (700) connected in a single row array; the placement assembly (700) comprises a square frame (701) for mounting the sputum cup (8), a side handle (702) arranged on the side of the square frame (701), a side window (703) opened on the left and right sides of the square frame (701), and a handle body installation opening (704) opened through the side handle (702); The sputum cup (8) comprises a main cup body (801), a cup mouth slide groove (802) provided at the opening of the main cup body (801), a sliding cover (803) horizontally slidably connected along the cup mouth slide groove (802), and an operating handle (804) vertically provided on the lower surface of the outer side of the sliding cover (803).

2. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 1 is characterized in that: The digestive fluid filling unit (2) comprises: An operating position control component (210) disposed obliquely downward at the filling function area (101) of the main body (1), an operating quantity control component (220) disposed at the output end of the operating position control component (210), and an operating gun (230) mounted on the operating position control component (210) and the operating quantity control component (220); The operating position control assembly (210) comprises a first driving motor (211) fixedly connected to the main base (1), a first driving screw (212) coaxially fixedly connected to the first driving motor (211), a first nut block (213) threadedly connected to the first driving screw (212), a carrier (214) fixed on the first nut block (213), and a clamping portion (215) provided on the upper surface of the carrier (214) for clamping the operating gun (230); The operating gun (230) comprises a main body (231) clamped by a clamping portion (215), a push handle (232) clamped by the operating amount control component (220) and realizing push amount control, and a gun head (233) whose front end position is controlled by the operating position control component (210).

3. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 2, characterized in that: The operation amount control component (220) comprises: A second drive motor (221) fixed to the rear side of the carrier (214), a second drive screw (222) coaxially and fixedly connected to the second drive motor (221), a second nut block (223) maintaining threaded linear transmission with the second drive screw (222), and a bayonet provided on the upper surface of the second nut block (223) for clamping the push handle (232).

4. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 1, characterized in that: The clamping control assembly (410) comprises: A clamping control slide (411) fixed to the bottom surface of the clamping functional area (401) of the main housing (400), a clamping control drive motor (412) fixed to the left side of the clamping control slide (411), a clamping control transmission screw (413) coaxially fixedly connected to the clamping control drive motor (412), a clamping control transmission block (414) provided on the clamping control transmission screw (413), a first base (415) fixedly connected to the clamping control transmission block (414), a second base (416) slidably connected to a horizontal mounting hole in the first base (415), and a clamping control drive screw (413) provided between the first base (415) and the second base (416). The invention relates to a forward pushing spring (4110), a cup body clamping plate (4111) fixed to the right end of the second base body (416) for clamping a batch of sputum cups placed in the tube group installation area (403), a non-slip rubber pad (4112) attached to the front side of the cup body clamping plate (4111), a cup handle clamping assembly (417) provided on the second base body (416) and used for clamping the sputum cup operating handle (804), a cup cap opening and closing control assembly (419) for controlling the cup handle clamping assembly (417) to slide horizontally, and a cup handle releasing assembly (418) for releasing the cup handle clamping assembly (417) from being clamped to the operating handle (804).

5. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 4, characterized in that: The cup handle clamping assembly (417) comprises: An initial clamping groove (4170) is provided on the upper surface of the second base (416) and is located on the left side of the cup body clamping plate (4111); a clamping slider (4171) is placed in the initial clamping groove (4170) in the initial state; a side lifting angle (4172) is protruded from the front and rear sides of the clamping slider (4171); a horizontal sliding surface (4173) is connected to the lower surface of the side lifting angle (4172) and can slide along the upper surface of the first base (415); a cup handle clamping groove (4174) is provided on the upper surface of the clamping slider (4171) for inserting the operating handle (804); a rubber layer (4175) is provided on the side of the cup handle clamping groove (4174); and a push angle (4176) is provided horizontally on the right side of the first base (415) and is consistent in height with the side lifting angle (4172) in the initial state of the clamping slider (4171).

6. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 5, characterized in that: The cup handle release assembly (418) comprises: A groove body (4181) vertically opened on the bottom surface of the initial card groove (4170), a pull-back spring (4182) fixedly connected to the bottom surface of the groove body (4181), and a pull-back rope (4183) fixedly connected to the upper end of the pull-back spring (4182); the upper end of the pull-back rope (4183) is fixedly connected to the card-engaging slider (4171).

7. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 6, characterized in that: The cup cap opening and closing control component (419) comprises: A magnet (4194) fixedly arranged on the engaging slider (4171), a transition groove (4191) opened on the left surface of the notch of the initial engaging groove (4170), a main groove (4192) maintained at the same height as the transition groove (4191) and opened on the upper surface of the first substrate (415), and a magnetic attraction device (4193) fixed on the left side of the main groove (4192) for controlling attraction or discharge to the magnet (4194).

8. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 1, characterized in that: The shaking unit (6) comprises: A shaking drive motor (601) is fixedly arranged at the output end of the internal and external transmission unit (5), and a connecting shaft (602) is coaxially and fixedly connected to the output end of the shaking drive motor (601), and the connecting shaft (602) is fixedly connected to the side of the tube group clamping control unit (4).

9. The sputum homogenization, shaking and sampling integrated pre-treatment equipment according to claim 1, characterized in that: The internal and external transmission unit (5) comprises: A transmission drive motor (501) is arranged at the bottom center of the main base (1), a vertical transmission screw (502) is coaxially fixedly connected to the output shaft of the transmission drive motor (501), and a transmission nut block (503) is threadedly connected to the vertical transmission screw (502); the shaking drive motor (601) is fixedly connected to the transmission nut block (503).

10. A method for using a sputum homogenization, shaking, and sampling integrated pre-treatment device, characterized in that: The steps include: S100: First, start the transmission drive motor (501), move the tube group clamping control unit (4) as a whole to the second pick-up and placement window (105), install the sputum cups (8) in batches into the sputum cup tube rack (7), keep the main cup body (801) placed in the square frame (701), and place the operating handle (804) in the handle body installation opening (704); S200: Controlling the clamping drive motor (412) to rotate, thereby driving the first base (415) to move horizontally to the right, and under the action of the forward push spring (4110), the second base (416) is pushed to move to the right, and then the rubber cushion layer (4112) contacts and presses the sputum cup main cup body (801); S300: Continue to control the clamping control driving motor (412) to rotate. At this time, the second base (416) no longer moves forward and maintains a tight state on the sputum cup. At the same time, the first base (415) continues to move to the right, so that the front push-up angle (4176) contacts the side lifting angle (4172) and pushes the clamping slider (4171) upward, so that it finally disengages from the initial clamping groove (4170), so that the operating handle (804) above the clamping slider (4171) is clamped into the cup handle clamping groove (4174), and the horizontal sliding surface (4173) of the clamping slider (4171) is flush with the upper surface of the first base (415); then, the internal and external conveying units (5) are controlled to move in the opposite direction, so that the tube group clamping control unit (4) is brought to the main working area (103) to prepare for the pre-processing operation; S400: By controlling the magnetic attraction device (4193) to be energized, the magnet (4194) is attracted, so that the clamping slide block (4171) slides horizontally to the left along the transition slide groove (4191) and the main slide groove (4192), and then the operating handle (804) on the clamping slide block (4171) is pulled to the left, so that the sputum cup (8) is opened; S500: by controlling the first drive motor (211) in the digestive liquid filling unit (2), the front end of the operating gun (230) is brought above the sputum cup, and by controlling the second drive motor (221) in the digestive liquid filling unit (2), the amount of digestive liquid injected is controlled; S600: The electromagnetic suction device (4193) is turned in the reverse direction to generate a repulsive force on the magnet (4194), thereby pushing the clamping slider (4171) to the right, thereby closing the sputum cup cover; S700: by controlling the shaking drive motor (601), the tube group clamp control unit (4) is shaken left and right, thereby achieving a mixing operation of the sputum and digestive fluid in the sputum cup, and then standing for a fixed time; S800: By controlling the forward electromagnetic suction device (4193) to generate an attractive force on the magnet (4194), the sputum cup lid is opened, and the first drive motor (211) and the second drive motor (221) in the sample suction unit (3) are started to achieve quantitative suction of the sample in the sputum cup; then, the sputum cup lid is controlled to be closed; S900: The tube group clamping control unit (4) is pushed out from the second access window (105) through the internal and external conveying unit (5) to facilitate the staff to take it out.

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