Method for producing lily bulbs by vegetative propagation
Patent Information
- Application Number
- CN202510155572.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2026-08-21
- Estimated Expiration
- 2045-02-12
AI Technical Summary
然而,并不是所有的百合属植物都能形成珠芽,目前已知有四种野生种百合即卷丹(L.lancifolium)、淡黄花百合(L.sulphureum)、通江百合(L.sargentiae)和珠芽百合(L.bulbiferum)在茎中的腋芽处能自然形成珠芽,但此种繁殖方式只适用于存在珠芽发生的部分百合属材料
[0013]本发明百合活体繁殖籽球的方法突破传统方式,另辟蹊径,通过外源调控使植株茎秆获得珠芽用来生产百合籽球,以大幅降低百合种球的繁殖成本,对我国的百合种业将起到很好的推动作用。
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Figure CN119856643B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of garden plant cultivation and maintenance, and in particular to a method for the live propagation of lily bulbs. Background Technology
[0002] As one of the world's most famous bulbous flowers and one of the five major cut flowers, the lily possesses multiple values—medicinal, ornamental, and edible—giving it a very broad development prospect in the flower industry. The development of the lily industry and the continuous expansion of market demand have placed higher demands on the quality and quantity of lily bulbs. my country's lily seed industry is basically in its infancy.
[0003] Bulbils are a form of reproduction in lilies, consisting of above-ground organs that grow within axillary buds and originate from axillary meristems, similar to tillers in Arabidopsis thaliana and rice. Bulbils and bulbs are essentially the same reproductive organs of lilies, but they differ in their location. Bulbs grow underground, while bulbils grow above ground. After maturing, the bulbils fall to the ground and begin to develop into bulbs. However, not all lily species can form bulbils. Currently, four wild lily species—L. lancifolium, L. sulphureum, L. sargentiae, and L. bulbiferum—are known to naturally form bulbils at the axillary buds of their stems. However, this method of reproduction is only applicable to certain lily species where bulbil formation is present.
[0004] Obtaining bulbs through scale cuttings is currently the main method of lily propagation, but the price of lily bulbs has remained high and is increasing year by year, causing the cost of propagation to rise daily. Summary of the Invention
[0005] The technical problem to be solved by the present invention is to provide a method for the live propagation of lily bulbs that is low in cost, easy to operate, and has excellent results.
[0006] The method for propagating lily bulbs from live organisms according to the present invention includes the following steps:
[0007] After 3-4 weeks of germination, spray the seedlings with an amino acid water-soluble fertilizer containing chlorpyrifos or thiamethoxam. Two months after the lilies bloom, the bulbils growing from the plant stems will mature. Cut the plant stems from the base, cut them into small sections according to the planting container, bury them in nutrient soil, and water them for cultivation.
[0008] The method for propagating lily bulbs by live organisms according to the present invention, wherein the dilution ratio of the amino acid water-soluble fertilizer is 1:1000, and after dilution, chlorpyrifos or thiamethoxam is added, wherein the volume ratio of chlorpyrifos to the diluted amino acid water-soluble fertilizer is 1:50, and the volume ratio of thiamethoxam to the diluted amino acid water-soluble fertilizer is 1:50.
[0009] The method for propagating lily bulbs by live organisms according to the present invention, wherein the circumference of the lily bulbs is 6-8 cm, they are cultivated in a greenhouse, with normal watering management, and sprayed 3-4 weeks after germination, wherein the spraying is foliar spraying.
[0010] The method for propagating lily bulblets by live organisms according to the present invention involves spraying once more after a three-week interval, and then spraying again two months after the lily blooms, when the bulbils growing from the plant stems mature.
[0011] The method for propagating lily bulblets by live organisms according to the present invention involves cutting the plant stem from the root after the bulbils mature. When planting, the stem with bulbils is cut into 7-10cm segments and placed horizontally in nutrient soil. The segments are covered with 2-3cm of nutrient soil and watered with water containing rooting powder to promote root growth. After 2 weeks, the bulbils develop roots, and after 1 month, they can grow scale leaves. After 1-2 years of planting, they grow into lily bulblets.
[0012] The method for propagating lily bulbs using live organisms in this invention differs from existing technologies in that:
[0013] This invention provides a method for the live propagation of lily bulbs that breaks with traditional methods and takes a different approach. By using exogenous regulation to induce bulbils in the plant stems for the production of lily bulbs, the propagation cost of lily bulbs is significantly reduced, which will greatly promote the development of my country's lily seed industry.
[0014] The method of this invention can harvest bulbils and seed bulbs. The size and weight of the seed bulbs are no different from the control, indicating that the nutrients and energy consumed by bulbil growth can be compensated by the use of amino acid water-soluble fertilizer. This shows that the method of this invention can obtain bulbils for lily propagation without affecting the original yield of lilies.
[0015] The method for propagating lily bulbs using live organisms according to the present invention will be further described below with reference to the accompanying drawings. Attached Figure Description
[0016] Figure 1 The lily seedlings in this embodiment of the invention are 3-4 weeks old.
[0017] Figure 2 In this embodiment of the invention, bulbils grow from the leaf axils of the lily plant stem, wherein: CK: untreated; 1: amino acid water-soluble fertilizer; 2: amino acid aqueous solution + chlorpyrifos; 3: amino acid water-soluble fertilizer + thiamethoxam.
[0018] Figure 3 The statistical results of lily bulb harvesting using the method of this invention;
[0019] Figure 4The diagram shows the planting and growth of lily bulbils using the method of this invention, wherein: left: cut stem segment; middle: bulbils taking root; right: bulbils growing scale leaves;
[0020] Figure 5 The image shows the effect of spraying amino acid water-soluble fertilizer + chlorpyrifos on some series of lily varieties using the method of this invention. The top row of the image, from left to right, shows Pol Jessica, Losela's Dream, Sheikh Rashad, Isabella, and Zambezi; the bottom row, from left to right, shows Pink Flight, Tiger Baby, Two-Person Game, Vanilla Scent, and Comfortable Waltz. Detailed Implementation
[0021] 1. Experimental Materials
[0022] Lily bulbs, amino acid water-soluble fertilizer, chlorpyrifos, thiamethoxam, and "Ruqing" brand substrate nutrient soil.
[0023] 2. Experimental Methods
[0024] Plant the small bulbs of 'Shuttle' lily (6-8cm in circumference) in nutrient soil, cultivate them in a greenhouse, and manage and water them normally. 3-4 weeks after germination... Figure 1 Foliar spraying with an amino acid water-soluble fertilizer containing chlorpyrifos or thiamethoxam (ensuring complete coverage of the entire plant) is recommended. The amino acid water-soluble fertilizer should be diluted 1:1000 by volume. After dilution, add chlorpyrifos or thiamethoxam at a ratio of 1:50 for both. Repeat the spraying after 3 weeks. Two to three months after flowering, when the bulbils on the stems mature and can root normally, cut the stems from the base into 7-10cm segments, depending on the planting container. Place these segments horizontally in the potting soil and cover with 3-4cm of potting soil. Water with water containing rooting powder to promote rooting and sprouting of the bulbils.
[0025] 3. Experimental Results
[0026] 3.1 Effects of amino acid water-soluble fertilizer, chlorpyrifos, and thiamethoxam on the growth of lily bulbils
[0027] Three weeks after the lily bulbs sprout, foliar spraying was applied using fertilizers #1 (amino acid water-soluble fertilizer), #2 (amino acid water-soluble fertilizer + chlorpyrifos), and #3 (amino acid water-soluble fertilizer + thiamethoxam). Treatments #2 and #3, which contain chlorpyrifos and thiamethoxam, resulted in bulbils beginning to sprout from the leaf axils of the stems after 3-4 weeks. Figure 2 The growth of bulbils will delay flowering by about one week. Bulb harvest statistics are shown in the table below. Figure 3The results showed that treatment #1 significantly increased bulb yield, with a marked increase in both bulb size and circumference. Treatments #2 and #3 both yielded bulbils and stem bulbs, with no difference in bulb size and weight compared to the control. This indicates that the nutrients and energy consumed by bulbil growth can be compensated for by the use of amino acid water-soluble fertilizer. Therefore, this method can obtain bulbils for lily propagation without affecting the original lily yield.
[0028] Table 1. Effects of amino acid water-soluble fertilizer, chlorpyrifos, and thiamethoxam on bulb weight
[0029]
[0030] Table 2. Effects of amino acid water-soluble fertilizer, chlorpyrifos, and thiamethoxam on bulb circumference after treatment.
[0031]
[0032] 3.2 Cultivation of Lily Bulbuls
[0033] After lilies have been treated with amino acid water-soluble fertilizer and chlorpyrifos or thiamethoxam, the bulbils on the stems will grow for 2-3 months and are then ready for planting. When planting, cut the stems with bulbils into 7-10cm sections, place them horizontally in 'Ruqing' brand potting soil, cover them with 3-4cm of potting soil, and water with water containing rooting powder to promote root growth. Roots will develop on the bulbils after 2 weeks, and scale leaves will appear after 1 month. Figure 4 After 1-2 years of planting, it can grow into lily seed bulbs.
[0034] 3.3 Spraying effects on different lily varieties
[0035] The above experiments demonstrate that both chlorpyrifos and thiamethoxam can promote the germination of bulbils in the leaf axils of lily seedlings when used. This method has been tested on multiple lily varieties and has consistently promoted the formation of bulbils in the leaf axils of their stems. Figure 5 The effectiveness rate is over 90%.
[0036] The specific varieties of lilies are as follows:
[0037] Asian Lilies: Losela's Dream, Tribal Dance, Nitty's Pride, Biting Juan, Pol Jessica, Walker, Summer Sunshine, Lollipop, Happy Memories, Selection, Pink Morpho Butterfly, Sheikh Rashad;
[0038] Pollen-Free Series: Two-Player Game, Vanilla, Spotted, Cozy Waltz, Comfortable Sunshine;
[0039] Spotted Series: Strawberry Feast, Pink Flight, Tiger Cub;
[0040] Speaker Series: White Planet;
[0041] Gun series: Cali, Devas;
[0042] Oriental Series: Night Fragrant Mountain, Isabella, Idisa, Siberia, Zambezi.
[0043] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims
1. A method for propagating lily bulbs from live organisms, characterized in that: Includes the following steps: After the lily bulbs sprout, spray them with an amino acid water-soluble fertilizer containing chlorpyrifos or thiamethoxam 3-4 weeks after they sprout. Two months after the lilies bloom, the bulbils that grow from the plant stems will mature. Cut the plant stems off the roots, cut them into small sections according to the planting container, bury them in nutrient soil, and water them for cultivation. in: The amino acid water-soluble fertilizer is diluted by a volume ratio of 1:1000. After dilution, chlorpyrifos or thiamethoxam is added. The volume ratio of chlorpyrifos to the diluted amino acid water-soluble fertilizer is 1:50, and the volume ratio of thiamethoxam to the diluted amino acid water-soluble fertilizer is 1:
50. The lily bulbs have a circumference of 6-8 cm. They are grown in a greenhouse with normal watering and management. Spraying is carried out 3-4 weeks after sprouting. The spraying is foliar spraying. Spray again after 3 weeks. Two months after the lilies bloom, the bulbils growing from the plant stems will mature. The lilies are selected from the Trumpet series, Gun series, or Oriental series.
2. The method for propagating lily bulbs from live organisms according to claim 1, characterized in that: After the bulbils mature, cut the plant stem from the root. When planting, cut the stem with bulbils into 7-10cm sections, place them horizontally in the potting soil, cover them with 3-4cm of potting soil, and water with water containing rooting powder to promote root growth. After 2 weeks, the bulbils will grow roots, and after 1 month, they will grow scale leaves. After 1-2 years of planting, they will grow into lily bulbs.
Citation Information
Patent Citations
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