Application of artemisia dubia extract in crop growth promotion, crop yield increase or root rot prevention and treatment

Abstract

The invention relates to application of an artemisia dubia extract in crop growth promotion, crop yield increase or root rot prevention and treatment. The artemisia dubia extract is found to be capable of promoting growth of crops, increasing the yield of the crops and preventing and treating root rot, and when the artemisia dubia extract is prepared into a seed treatment suspending agent to coat soybean seeds before sowing, soybean growth can be effectively promoted, the soybean yield can be increased, and the soybean root rot can be prevented and treated.

Description

Technical Field

[0001] The invention relates to the field of growth promoters or biocides, and in particular to application of an Artemisia oxtail extract in promoting crop growth, increasing crop yield or preventing and controlling root rot. Background Art

[0002] Soybean contains rich high-quality protein and unsaturated fatty acids. It is a common food with high nutritional value in daily life. Its global planting area ranks fourth in the world after rice, wheat and corn. In order to increase supply, it is necessary to increase soybean production. In addition, soybean root rot is a soil-borne disease with the characteristics of wide distribution, severe damage, many pathogens and difficult prevention and control. It seriously affects the yield and quality of soybeans and brings huge losses to soybean production. How to effectively prevent and control soybean root rot is of great significance to ensure soybean production and quality safety. Summary of the invention

[0003] One of the present inventions provides an application of an Artemisia selengensis extract in promoting crop growth, increasing crop yield or preventing and controlling root rot. The Artemisia selengensis extract is extracted by the following method: mixing the aerial part of Artemisia selengensis with 95% ethanol in a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein the ultrasonic frequency is 40 KHz, the ultrasonic power is 160 to 220 W, for example, 180 W, the cyclic ultrasonic extraction time is 2 to 3 h, for example, 2.5 h, the temperature is 30 to 50 degrees Celsius, for example, 40 degrees Celsius, and after natural cooling, the extract is filtered, the filtrate is retained after filtration, and the filter residue is repeatedly extracted for more than 2 times, the filtrates extracted several times are combined, and concentrated to an extract to obtain the Artemisia selengensis extract.

[0004] In a specific embodiment, the application is the application of Artemisia oxtail extract in promoting soybean growth or increasing soybean yield.

[0005] In a specific embodiment, the root rot is soybean root rot.

[0006] The second aspect of the present invention provides a composition, which includes an Artemisia selengensis extract and an insecticide-acceptable carrier. The Artemisia selengensis extract is extracted by the following method: the aerial part of Artemisia selengensis is mixed with 95% ethanol in a ratio of 1g: (10 to 30)ml, and then ultrasonic extraction is performed, wherein the ultrasonic frequency is 40KHz, the ultrasonic power is 160 to 220W, for example, 180W, the cyclic ultrasonic extraction time is 2 to 3h, for example, 2.5h, the temperature is 30 to 50 degrees Celsius, for example, 40 degrees Celsius, and the Artemisia selengensis extract is obtained by natural cooling and filtration. After filtration, the filtrate is retained, and the residue is repeatedly extracted for more than 2 times. The filtrates extracted several times are combined and concentrated to an extract to obtain the Artemisia selengensis extract.

[0007] In a specific embodiment, the pesticide-acceptable carrier includes at least one of a dispersing wetting agent, a film-forming agent, a thickening agent, a preservative, an antifreeze agent and water.

[0008] In a specific embodiment, based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 1% to 5%, the content of the dispersing wetting agent is 1.5% to 7%, the content of the film-forming agent is 3% to 7%, the content of the thickener is 0.2% to 6%, the content of the preservative is 0.1% to 1%, the content of the antifreeze agent is 3% to 5%, and the content of water is 49% to 71.2%.

[0009] In a specific embodiment, the dispersing wetting agent is Morwet EFW and / or Morwet IP.

[0010] In a specific embodiment, the film-forming agent is carboxymethyl cellulose.

[0011] In a specific embodiment, the thickener is xanthan gum and / or magnesium aluminum silicate.

[0012] In a specific embodiment, the preservative is sorbic acid.

[0013] In a specific embodiment, the antifreeze agent is ethylene glycol.

[0014] In a specific embodiment, based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 1% to 5%, the content of the Morwet EFW is 0.5% to 3%, the content of the Morwet IP is 1% to 4%, the content of the carboxymethyl cellulose is 3% to 7%, the content of the xanthan gum is 0.1% to 3%, the content of the magnesium aluminum silicate is 0.1% to 3%, the content of the sorbic acid is 0.1% to 1%, the content of the ethylene glycol is 3% to 5%, and the content of the water is 49% to 71.2%.

[0015] In one embodiment, the composition is prepared by the following steps: 1) Prepare carboxymethyl cellulose solution; 2) Prepare xanthan gum solution; 3) After the dispersing wetting agent is fully dissolved in water, the Artemisia oxtail extract, magnesium aluminum silicate, antifreeze agent and preservative are added, and the mixture is fully mixed to obtain a first material to be ground, and the first material to be ground is first ground at a temperature below 25 degrees Celsius to obtain a first ground material; 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding is performed on the second grinding material at a temperature below 25 degrees Celsius until 95% of the particles are smaller than 5 microns, thereby obtaining the seed treatment suspension.

[0016] In a specific embodiment, the first grinding is performed with zirconium oxide beads having a particle size of 1 to 1.2 mm.

[0017] In a specific embodiment, the second grinding is performed using zirconium oxide beads with a particle size of 0.8 to 1 mm.

[0018] In a specific embodiment, the first material to be ground is first ground at 5 to 15 degrees Celsius.

[0019] In one embodiment, the first grinding takes 2 to 5 hours.

[0020] In a specific embodiment, the second material to be ground is subjected to a second grinding at 5 to 15 degrees Celsius.

[0021] In one specific embodiment, the first grinding is performed in a sand mill, and the zirconium oxide beads with a particle size of 1 to 1.2 mm occupy 60% to 75% of the volume of the grinding jar of the sand mill.

[0022] In one specific embodiment, the second grinding is performed in a sand mill, and the zirconium oxide beads with a particle size of 0.8 to 1 mm occupy 60% to 75% of the volume of the grinding jar of the sand mill.

[0023] The third aspect of the present invention provides the use of the composition according to any one of the second aspects of the present invention in promoting crop growth, increasing crop yield or preventing and controlling root rot.

[0024] Beneficial effects of the present invention: The present invention finds that the Artemisia oxtail extract can promote the growth of crops, increase crop yields and prevent and treat root rot. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The results of various indicators of the seed treatment suspension concentrates of Examples 2 to 5 are shown.

[0026] Figure 2 The effects of the seed treatment suspensions of Examples 2 to 4 on indoor soybean growth are shown.

[0027] Figure 3 The results show the effects of the seed treatment suspensions of Examples 2 to 4 on the control of soybean root rot in the field and on soybean yield. DETAILED DESCRIPTION

[0028] The above contents of the present invention are further described in detail below in the form of preferred implementation cases, but they do not constitute a limitation to the present invention.

[0029] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased through commercial channels.

[0030] Morwet EFW and Morwet IP were purchased from Nanjing Jierun Technology Co., Ltd.

[0031] Carboxymethyl cellulose (CMC) was purchased from Shandong Chengxin Chemical Technology Co., Ltd.

[0032] Xanthan gum was purchased from Guangdong Hengtian Biotechnology Co., Ltd.

[0033] Magnesium aluminum silicate was purchased from Wuhan Jiyesheng Chemical Co., Ltd.

[0034] Sorbic acid was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0035] Ethylene glycol was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Preparation of Artemisia oxtail extract Example 1

[0036] Take the aboveground part of Artemisia selengensis and use ultrasonic extraction method. Use a grinder to crush Artemisia selengensis, mix the aboveground part of Artemisia selengensis with 95% ethanol at a ratio of 1g:30ml in an ultrasonic extractor for ultrasonic extraction, wherein the ultrasonic frequency is 40KHz, the ultrasonic power is 180W, the cyclic ultrasonic extraction time is 2.5h, the temperature is 40 degrees Celsius, and the temperature is naturally cooled and filtered. After filtering, the filtrate is retained, and the filter residue is soaked again with 95% ethanol at a ratio of 1g:30ml. After repeated extraction 3 times, the filtrates of several extractions are combined, and the filtrates are concentrated to an extract by a rotary evaporator under reduced pressure to obtain an Artemisia selengensis extract.

[0037] High performance liquid chromatography (HPLC) determined that the extract of Artemisia oxtailii contained 31% capillarin diacetyl ketone.

[0038] Based on the determination of nitrogen, phosphorus and potassium in plants (NY / T 2017-2011 molybdenum antimony anti-absorption spectrophotometry), the phosphorus element in the Artemisia selengensis extract was detected, and the result was no detection. Preparation of seed treatment suspension concentrate Example 2

[0039] 1) Add 100g of water into the mixing tank. With the stirring device turned on, slowly and evenly sprinkle 50g of carboxymethyl cellulose (CMC) into the mixing tank, stirring continuously. When the carboxymethyl cellulose (CMC) is evenly dispersed in the water and there are no obvious large lumps, stop stirring to obtain carboxymethyl cellulose liquid.

[0040] 2) Slowly add 10 grams of xanthan gum into 100 grams of water while stirring to obtain xanthan gum solution.

[0041] 3) 630 g of water is added to a container, and then 10 g of Morwet EFW and 20 g of Morwet IP are added to fully dissolve; then 25 g of Artemisia oxtail extract, 10 g of magnesium aluminum silicate, 40 g of ethylene glycol, and 5 g of sorbic acid are added, and the mixture is fully mixed to obtain a first material to be ground; then the first material to be ground and zirconia beads with a particle size of 1 mm are added to a vertical sand mill, and the first grinding is performed at 15 degrees Celsius, wherein the zirconia beads account for 70% of the volume of the tank, the rotation speed of the first grinding is 2000 rpm, and the grinding is performed for 2 hours to obtain a first ground material.

[0042] 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding material and zirconia beads with a particle size of 0.8 mm are added to a vertical sand mill, and a second grinding is performed at 15 degrees Celsius, wherein the zirconia beads account for 70% of the volume of the tank, and the rotation speed of the second grinding is 2000 rpm. During the grinding, a small amount is taken out and the particle size is detected by a laser particle size analyzer. The second grinding is stopped when 95% of the particles are less than 5 microns to prepare a seed treatment suspension. Example 3

[0043] 1) Add 100g of water into the mixing tank. With the stirring device turned on, slowly and evenly sprinkle 30g of carboxymethyl cellulose (CMC) into the mixing tank, stirring continuously. When the carboxymethyl cellulose (CMC) is evenly dispersed in the water and there are no obvious large lumps, stop stirring to obtain carboxymethyl cellulose liquid.

[0044] 2) Slowly add 1 gram of xanthan gum into 100 grams of water while stirring to obtain xanthan gum solution.

[0045] 3) 712 g of water is added to a container, and then 5 g of Morwet EFW and 10 g of Morwet IP are added to fully dissolve; then 10 g of Artemisia oxtail extract, 1 g of magnesium aluminum silicate, 30 g of ethylene glycol, and 1 g of sorbic acid are added, and the mixture is fully mixed to obtain a first material to be ground; then the first material to be ground and zirconium oxide beads with a particle size of 1.2 mm are added to a vertical sand mill, and a first grinding is performed at 25 degrees Celsius, wherein the zirconium oxide beads account for 60% of the volume of the tank, the rotation speed of the first grinding is 2000 rpm, and the first grinding is performed for 2 hours to obtain a first ground material.

[0046] 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding material and zirconia beads with a particle size of 1.0 mm are added to a vertical sand mill, and a second grinding is performed at 25 degrees Celsius, wherein the zirconia beads occupy 60% of the volume of the tank, and the rotation speed of the second grinding is 2000 rpm. During the grinding, a small amount is taken out and the particle size is detected by a laser particle size analyzer. The second grinding is stopped when 95% of the particles are less than 5 microns to prepare a seed treatment suspension. Example 4

[0047] 1) Add 100g of water into the mixing tank. With the stirring device turned on, slowly and evenly sprinkle 70g of carboxymethyl cellulose (CMC) into the mixing tank, stirring continuously. When the carboxymethyl cellulose (CMC) is evenly dispersed in the water and there are no obvious large lumps, stop stirring to obtain carboxymethyl cellulose liquid.

[0048] 2) Slowly add 30 grams of xanthan gum into 100 grams of water while stirring to obtain xanthan gum solution.

[0049] 3) Take 490 grams of water and add it into a container, then add 30 grams of Morwet EFW and 40 grams of Morwet IP, and fully dissolve them; then add 50 grams of Artemisia oxtail extract, 30 grams of magnesium aluminum silicate, 50 grams of ethylene glycol, and 10 grams of sorbic acid, and mix them evenly to obtain a first material to be ground, and then add the first material to be ground and zirconium oxide beads with a particle size of 1 mm into a vertical sand mill, and perform a first grinding at 5 degrees Celsius, wherein the zirconium oxide beads account for 75% of the volume of the tank, the rotation speed of the first grinding is 2000 rpm, and the grinding is performed for 2 hours to obtain a first ground material.

[0050] 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding material and zirconia beads with a particle size of 0.8 mm are added to a vertical sand mill, and a second grinding is performed at 5 degrees Celsius, wherein the zirconia beads occupy 75% of the volume of the tank, and the rotation speed of the second grinding is 2000 rpm. During the process, a small amount is taken out to detect the particle size of the particles with a laser particle size analyzer, and the second grinding is stopped when 95% of the particles are less than 5 microns to prepare a seed treatment suspension. Example 5

[0051] 1) Add 100g of water into the mixing tank. With the stirring device turned on, slowly and evenly sprinkle 50g of carboxymethyl cellulose (CMC) into the mixing tank, stirring continuously. When the carboxymethyl cellulose (CMC) is evenly dispersed in the water and there are no obvious large lumps, stop stirring to obtain carboxymethyl cellulose liquid.

[0052] 2) Slowly add 2 grams of xanthan gum into 100 grams of water while stirring to obtain xanthan gum solution.

[0053] 3) 603 g of water is added to a container, and then 10 g of Morwet EFW and 20 g of Morwet IP are added to fully dissolve; then 60 g of Artemisia oxtail extract, 10 g of magnesium aluminum silicate, 40 g of ethylene glycol, and 5 g of sorbic acid are added and mixed uniformly to obtain a first material to be ground, and then the first material to be ground and zirconia beads with a particle size of 1 mm are added to a vertical sand mill, and a first grinding is performed at 15 degrees Celsius, wherein the zirconia beads account for 70% of the volume of the tank, the rotation speed of the first grinding is 2000 rpm, and the grinding is performed for 2 hours to obtain a first ground material.

[0054] 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding material and zirconia beads with a particle size of 0.8 mm are added to a vertical sand mill, and a second grinding is performed at 15 degrees Celsius, wherein the zirconia beads account for 70% of the volume of the tank, and the rotation speed of the second grinding is 2000 rpm. During the grinding, a small amount is taken out and the particle size is detected by a laser particle size analyzer. The second grinding is stopped when 95% of the particles are less than 5 microns to prepare a seed treatment suspension. Determination of performance indexes of seed treatment suspension concentrate

[0055] The control item indicators of the seed treatment suspension of Examples 2 to 5 were analyzed, and the results are shown in Figure 1 .

[0056] Depend on Figure 1 It can be seen that the heat storage stability of Example 5 does not meet the standard, and the test results of various indicators of the seed treatment suspension of Examples 2 to 4 all meet the standard, and the suspension rate, dumping property and other indicators of Example 2 are better. Therefore, Examples 2 to 4 are selected for the next step of the experiment. Application Example 1: Indoor Effects on Soybean Growth

[0057] The soybean variety used for the test was Henong 75.

[0058] The experiment was conducted in a greenhouse with a room temperature of 23 degrees Celsius, a relative humidity of 65%, LED light supplementation, and alternating light and darkness for 12 hours.

[0059] Soak the soybean seeds in 75wt% ethanol aqueous solution for 30s and pour them out, rinse them with sterile water 3 times; soak them in 1wt% sodium hypochlorite aqueous solution for 1.5min, stir them continuously during the period to make the seeds fully merge with the sodium hypochlorite aqueous solution, and then rinse them repeatedly with sterile water 3 times to obtain pretreated soybean seeds. The seed treatment suspension of Examples 2 to 4 and the pretreated soybean seeds are mixed in a mass ratio of 1:100, and then dried to obtain coated soybean seeds. The coated soybeans are sown in a flowerpot containing sterilized nutrient soil. Among them, the sterilized nutrient soil is mixed with sterilized vermiculite in a volume ratio of 1:1, the flowerpot specifications are 11cm in diameter and 9cm in height, 2 soybean seeds per pot, 12 pots per treatment, repeated 4 times, and the pretreated soybean seeds without coating are used as blank controls. After 21d, 7 plants were selected for each treatment and each repetition to measure the four indicators of plant height, root length, aboveground fresh weight and underground fresh weight of soybean plants, and then the promotion rate of each indicator was calculated based on the following indicator promotion rate.

[0060] Index promotion rate = (index value of coating treatment group - index value of blank control group) × 100% / index value of blank control group.

[0061] Then, Excel 2021 software was used for data statistics, SPSS 25.0 software was used for statistical analysis of the experimental data, and Duncan's new multiple range method was used for significant difference test. The results are shown in Figure 2 .

[0062] Depend on Figure 2 It can be seen that the emergence rates of Examples 2 to 4 are all above 90%, and the emergence rates are good; compared with the blank control group, Example 2 has a promoting effect of 19% on the height of soybean plants, a promoting effect of 36% on the root length of soybean plants, a promoting effect of 40% on the fresh weight of the aboveground part, and a promoting effect of 60% on the fresh weight of the underground part; Example 3 has a promoting effect of 14% on the height of soybean plants, a promoting effect of 21% on the root length of soybean plants, a promoting effect of 29% on the fresh weight of the aboveground part, and a promoting effect of 46% on the fresh weight of the underground part; Example 4 has a promoting effect of 14% on the height of soybean plants, a promoting effect of 22% on the root length of soybean plants, a promoting effect of 32% on the fresh weight of the aboveground part, and a promoting effect of 51% on the fresh weight of the underground part. It is shown that the oxtail wormwood extract seed treatment suspension of the present invention has a promoting effect on the emergence rate, plant height, root length and fresh weight of soybeans, and Example 2 has the best promoting effect on soybean growth. Application Example 2: Field control of soybean root rot and its impact on soybean yield

[0063] The experiment was carried out according to GB / T17980.88-2004 “Guidelines for Field Efficacy Tests of Pesticides (II) Part 88: Fungicides for Control of Soybean Root Rot”.

[0064] The experiment was conducted in Yonghe Village, Yongfeng Town, Qinggang County, Suihua City, Heilongjiang Province. The previous crop was soybeans, and root rot was more serious in previous years. The cultivation conditions of all experimental plots were consistent and in line with local scientific agricultural practices (GAP). A randomized block design was used, with a plot area of ​​20m 2 , one plot was one replicate, and each treatment was repeated 4 times.

[0065] Target of prevention and control: soybean root rot.

[0066] Test variety: The soybean variety is Henong 75.

[0067] The seed treatment suspensions of Examples 2 to 4 were mixed with soybean seeds at a mass ratio of 1:100 to coat the soybean seeds, which were then dried to obtain coated soybean seeds, which were sown on May 14, 2024. Uncoated soybean seeds were used as blank controls.

[0068] Soybean root rot survey: The incidence of soybean root rot was investigated once every 10 days, 30 days, and 60 days after soybean seedlings emerged. The five-point sampling method was used for sampling, and 10 soybean plants were randomly dug at each point. The plants were graded according to the disease conditions at the root and stem base, and the control effect was calculated based on the disease index.

[0069] Grading method: Grade 0: There are no lesions at the base of the stem and the main fibrous roots of the plant; Grade 1: There are a few lesions at the base of the stem and the main root of the plant; Grade 3: There are many lesions at the base of the stem or the main root of the plant, and the area of ​​lesions accounts for 1 / 4~1 / 2 of the total area of ​​the stem and root; Grade 5: There are many and large lesions at the base of the stem and the main root of the plant, and the area of ​​lesions accounts for 1 / 2~3 / 4 of the total area of ​​the base of the stem and the root; Level 7: The lesions on the base of the plant stem and the main root are connected, forming a stem-wrapped phenomenon, but the root system is not dead; Level 9: The root system of the plant is necrotic, and the above-ground part of the plant wilts or dies.

[0070] The calculation of disease index and control effect is carried out in accordance with GB / T17980.88-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 88: Fungicide Control of Soybean Root Rot".

[0071] Investigation of soybean yield components: At harvest, 10 healthy representative plants were selected from each treatment, including the blank control, and the number of nodes, number of pods and 100-grain weight were investigated.

[0072] Crop safety observation: While conducting the soybean root rot investigation, observe whether the pesticide has any damage to the crop. If there is any damage, record the type and degree of damage. Also observe whether there is any impact on other organisms.

[0073] Excel 2021 software was used to perform statistical or calculation of disease index, control effect, number of nodes per plant, number of pods per plant, 100-grain weight and 100-grain weight growth. SPSS 25.0 software was used to perform statistical analysis on the test data. Duncan's new multiple range method was used for significant difference test. The results are shown in Figure 3 .

[0074] Figure 3 The results showed that after using the seed treatment suspensions of Examples 2 to 4, the disease index at 10d, 30d, and 60d after emergence was reduced compared with the blank control. 10d after emergence, the control effect of Example 2 on soybean root rot was 89.19%, the control effect of Example 3 on soybean root rot was 81.47%, and the control effect of Example 4 on soybean root rot was 84.55%; 30d after emergence, the control effect of Example 2 on soybean root rot was 93.41%, the control effect of Example 3 on soybean root rot was 81.32%, and the control effect of Example 4 on soybean root rot was 86.26%; 60d after emergence, the control effect of Example 2 on soybean root rot was 88.32%, the control effect of Example 3 on soybean root rot was 78.48%, and the control effect of Example 4 on soybean root rot was 83.20%. From the perspective of yield components, Examples 2 and 4 were significantly higher than the blank control in terms of plant node number, plant pod number, and 100-grain weight. In summary, coating soybean seeds with Artemisia selengensis extract seed treatment suspension has a good preventive and therapeutic effect on soybean root rot and has a significant yield-increasing effect.

[0075] Crop safety observation results: No obvious phytotoxicity to soybeans was found in any of the treatments during the entire crop growth period, and no impact was found on other organisms.

[0076] It can be seen from the above embodiments and application examples that the Artemisia oxysporum extract seed treatment suspension provided by the present invention can not only improve the emergence rate, promote the growth of plant height, root length and fresh weight of soybean plants, but also has a good prevention and control effect on soybean root rot and an effect of increasing yield.

Claims

1. Application of Artemisia selengensis extract in promoting crop growth, increasing crop yield or preventing and treating root rot, wherein the Artemisia selengensis extract is obtained by the following method: mixing the aerial part of Artemisia selengensis with 95% ethanol at a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein: The ultrasonic frequency is 40KHz, the ultrasonic power is 160 to 220W, the cyclic ultrasonic extraction time is 2 to 3h, the temperature is 30 to 50 degrees Celsius, and the extract is filtered after natural cooling. The filtrate is retained after filtration, and the residue is repeatedly extracted for more than 2 times. The filtrates from several extractions are combined and concentrated to an extract to obtain the Artemisia oxtail extract.

2. The use according to claim 1, characterized in that: The application is the application of the Artemisia oxtail extract in promoting soybean growth or increasing soybean yield.

3. The use according to claim 1, characterized in that: The root rot is soybean root rot.

4. A composition comprising an Artemisia oxysporum extract and an insecticide acceptable carrier, wherein the Artemisia oxysporum extract is obtained by the following method: mixing the aerial part of Artemisia oxysporum with 95% ethanol at a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein: The ultrasonic frequency is 40KHz, the ultrasonic power is 160 to 220W, the cyclic ultrasonic extraction time is 2 to 3h, the temperature is 30 to 50 degrees Celsius, and the extract is filtered after natural cooling. The filtrate is retained after filtration, and the residue is repeatedly extracted for more than 2 times. The filtrates from several extractions are combined and concentrated to an extract to obtain the Artemisia oxtail extract.

5. The composition according to claim 4, characterized in that The pesticide-acceptable carrier comprises at least one of a dispersing wetting agent, a film-forming agent, a thickening agent, a preservative, an antifreeze agent and water.

6. The composition according to claim 5, characterized in that Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 1% to 5%, the content of the dispersing wetting agent is 1.5% to 7%, the content of the film-forming agent is 3% to 7%, the content of the thickener is 0.2% to 6%, the content of the preservative is 0.1% to 1%, the content of the antifreeze agent is 3% to 5%, and the content of water is 49% to 71.2%.

7. The composition according to claim 5, characterized in that The dispersing wetting agent is Morwet EFW and / or Morwet IP; and / or The film-forming agent is carboxymethyl cellulose; and / or The thickener is xanthan gum and / or magnesium aluminum silicate; and / or The preservative is sorbic acid; and / or The antifreeze agent is ethylene glycol.

8. The composition according to claim 7, characterized in that Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 1% to 5%, the content of the Morwet EFW is 0.5% to 3%, the content of the Morwet IP is 1% to 4%, the content of the carboxymethyl cellulose is 3% to 7%, the content of the xanthan gum is 0.1% to 3%, the content of the magnesium aluminum silicate is 0.1% to 3%, the content of the sorbic acid is 0.1% to 1%, the content of the ethylene glycol is 3% to 5%, and the content of the water is 49% to 71.2%.

9. The composition according to claim 7 or 8, characterized in that The composition is prepared by the following steps: 1) Prepare carboxymethyl cellulose solution; 2) Prepare xanthan gum solution; 3) After the dispersing wetting agent is fully dissolved in water, the Artemisia oxtail extract, magnesium aluminum silicate, antifreeze agent and preservative are added, and the mixture is fully mixed to obtain a first material to be ground, and the first material to be ground is first ground at a temperature below 25 degrees Celsius to obtain a first ground material; 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding is performed on the second grinding material at a temperature below 25 degrees Celsius until 95% of the particles are smaller than 5 microns, thereby obtaining the seed treatment suspension.

10. Use of the composition according to any one of claims 4 to 9 in promoting crop growth, increasing crop yield or preventing and controlling root rot.

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