Application of herba schizonepetae extract in regulation of growth of crops

Abstract

The invention belongs to the technical field of natural product extraction and medicinal plants, and particularly relates to application of a herba schizonepetae extract in regulation of growth of crops. The herba schizonepetae extract comprises a herba schizonepetae inflorescence extract and / or a herba schizonepetae stem extract; the herba schizonepetae inflorescence extract comprises a herba schizonepetae inflorescence water extract, a herba schizonepetae inflorescence water extract and a herba schizonepetae inflorescence ethanol extract; the herba schizonepetae stem extract comprises a herba schizonepetae stem water extract, a herba schizonepetae stem water extract and a herba schizonepetae stem ethanol extract. The herba schizonepetae extract provided by the invention has a remarkable inhibition effect on lettuce seed germination and seedling growth, and has a remarkable promotion effect on barley and green vegetable seed germination and seedling growth. In vegetables and crops, the herba schizonepetae extract is used for inhibiting weeds around the required vegetables and crops and promoting the growth of the plants, and can be used for replacing chemical reagents and avoiding the adverse effects of the chemical reagents on the environment and the quality of fruits and vegetables.

Description

Technical Field

[0001] The invention belongs to the technical field of natural product extraction and medicinal plants, and particularly relates to application of schizonepeta extract in regulating the growth of crops. Background Art

[0002] Biodiversity refers to the variety and variability of life on Earth and plays a vital role in ecological functions. The comprehensive use of plant biodiversity is one of the ways to improve food security and sustainable agriculture. Species combinations such as multiple cropping, intercropping, rotation and mulching also have a positive impact on crop productivity and yield stability. Interactions between plant species may include the production and release of bioactive substances that directly or indirectly affect the growth and development of other organisms, a phenomenon known as allelopathy. The definition was later revised to refer to any process in which plants, microorganisms, viruses and fungi produce secondary metabolites that affect the growth and development of agricultural and biological systems, including positive and negative effects. Secondary metabolites related to allelopathy are released into the environment through volatilization, leaching, root exudation and decomposition of plant residues in the soil, and are called allelopathic chemicals. These allelopathic chemicals exist in different parts of various plants, such as leaves, roots, rhizomes, stems, flowers, pollen and fruits, and other natural ecological processes. Some plants are not dominant competitors in their natural habitats, but show strong successional advantages when introduced into new areas. Allelopathy is generally considered to be an important ecological factor determining the structure and composition of plant communities.

[0003] Despite recent progress in the development of agrochemicals for the control of pests and diseases in modern agriculture, diseases still exist. This is mainly due to pests, pathogens and weeds. Weeds are particularly destructive and more than 240 weed species have been found to have allelopathic effects on surrounding plants, both on the same species and on other crops and weed species. However, the need for chemical pest control in agriculture has been demonstrated by scientists in many different habitats around the world. Allelopathic effects have been reported for many plant species. For example, 84 out of 245 plant species from the Sino-Japanese flora showed significant inhibitory activity, of which 10 showed the strongest effects. An evaluation of the allelopathic potential of 83 Iranian medicinal plants found that Peganum harmala , Berberis vulgaris , Artemisia aucheri and Ferulago angulateThe root growth inhibition effect on lettuce was over 80%. An assessment of the allelopathic potential of medicinal plant species used in Ghana found that 75 out of 183 medicinal plants significantly inhibited the radicle growth of lettuce through leaf leachates. This is especially true considering that organic products have become increasingly popular over the past decade. Secondary metabolites of medicinal plants are believed to have strong allelopathic effects. In addition, it is easier to analyze medicinal plants to discover new natural compounds than to analyze other plants. Some bioactive substances in medicinal plants, such as ferulic acid, coumaric acid, vanillic acid, caffeic acid and chlorogenic acid, have been found to have inhibitory effects on plant growth. Therefore, there is a need to develop new strategies to exploit the allelopathic potential of medicinal plants in regulating plant growth and development. Summary of the invention

[0004] The purpose of the present invention is to develop new strategies for the allelopathic potential of medicinal plants in regulating plant growth and development, expand the application of medicinal plants in more fields, explore the allelopathic effects of different parts of Nepeta by sandwich method and plate assay under laboratory conditions, and provide the application of Nepeta extract in regulating the growth of crops. To achieve the above purpose, the present invention adopts the following technical scheme.

[0005] The present invention provides an application of a nepeta extract in regulating the growth of crops. The nepeta extract regulates the growth of the crops through allelopathic effects on the crops. The nepeta extract comprises a nepeta inflorescence extract and / or a nepeta stem extract.

[0006] The nepeta inflorescence extract comprises at least one of a nepeta inflorescence water extract, a nepeta inflorescence water extract and a nepeta inflorescence ethanol extract.

[0007] The Nepeta stem extract comprises at least one of Nepeta stem water extract, Nepeta stem water extract and Nepeta stem ethanol extract.

[0008] The schizonepeta inflorescence water extract, the schizonepeta inflorescence water extract and the schizonepeta inflorescence ethanol extract are all obtained by using schizonepeta inflorescence as materials and by adopting different extraction methods.

[0009] The schizonepeta stem water extract, the schizonepeta stem water extract and the schizonepeta stem ethanol extract are all obtained by using schizonepeta stem as materials and by adopting different extraction methods.

[0010] In order to develop a new strategy for the allelopathic potential of medicinal plants in regulating plant growth and development, the present invention provides an application of a schizonepeta extract in regulating the growth of crops, thereby expanding the application of the schizonepeta extract in more fields. Studies have found that the schizonepeta extract provided by the present invention can be used to regulate the growth of crops, thereby regulating the germination of crop seeds and / or the growth of seedlings.

[0011] Preferably, the crops include any one or more of lettuce, barley and greens. Using lettuce, barley and greens as representative plants of receptor plants for allelopathic research can facilitate the exploration of the effects of allelopathic substances on the growth of crop plants under different environmental stresses, and how the stress resistance mechanism of crop plants themselves interacts with allelopathic effects, providing ideas for breeding crop varieties with strong stress resistance.

[0012] Preferably, the Nepeta extract is used for regulating seed germination and seedling growth of the crops.

[0013] Preferably, the regulation includes promoting and / or inhibiting the seed germination and seedling growth of the crop.

[0014] Preferably, the method for preparing the water extract of Nepeta inflorescence comprises the following steps: After the schizonepeta inflorescence is dried, it is added with water and extracted for 48 hours at 4° C., and then filtered to collect the filtrate to obtain the schizonepeta inflorescence water extract.

[0015] The preparation method of the water extract of Nepeta stem comprises the following steps: The Nepeta stems are dried, and then extracted with water at 4° C. for 48 hours, and then filtered to collect the filtrate to obtain the Nepeta stem water extract.

[0016] The material ratio of the schizonepeta inflorescence to the water, and the material ratio of the schizonepeta stem to the water are both 8g:100mL. The material can also ensure the concentration and purity of the extract, and improve the extraction efficiency and economy. Specifically, the material ratio of the schizonepeta inflorescence to water, and the material ratio of the schizonepeta stem to water are both 8g:100mL. This optimized material ratio can ensure the concentration and purity of the extract. The appropriate material ratio can not only ensure sufficient solvent contact with the plant material, but also avoid the dilution effect caused by excessive solvent, thereby maintaining the concentration of the effective ingredients of the extract during the extraction process and improving the activity of the extract.

[0017] At the same time, the above material ratio can improve the extraction efficiency, reduce the use of solvents, and reduce production costs. At the same time, by accurately controlling the material ratio, the consistency of each extraction process can be ensured, and the stability and repeatability of the product can be improved, which is of great significance for large-scale production and application.

[0018] The preparation method of the water extract of the schizonepeta inflorescence and the water extract of the schizonepeta stem provided by the present invention can more effectively extract the active ingredients in schizonepeta through low-temperature water extraction and the above-mentioned material ratio, enhance its anti-inflammatory, antiviral, antioxidant and immunomodulatory effects, thereby effectively solving the related problems existing in the prior art.

[0019] Preferably, the method for preparing the water extract of Nepeta inflorescence comprises the following steps: After the schizonepeta inflorescence is dried, water is used as the first extraction solvent to perform the first extraction, and the extract is collected to obtain the schizonepeta inflorescence water extract.

[0020] The preparation method of the Nepeta stem water extract comprises the following steps: After the schizonepeta stems are dried, water is used as the first extraction solvent to perform a first extraction, and the extract is collected to obtain the schizonepeta stem water extract.

[0021] The material ratio of the Nepeta inflorescence to the first extraction solvent is 5 g:3 mL; the material ratio of the Nepeta stem to the first extraction solvent is 10 g:6 mL.

[0022] Preferably, the conditions for the first extraction are: extraction temperature 100° C., extraction pressure 10.34 MPa, and extraction time 4 min.

[0023] Preferably, the method for preparing the ethanol extract of Nepeta inflorescence comprises the following steps: After the schizonepeta inflorescence is dried, a second extraction is performed using 75% by volume ethanol as a second extraction solvent, and the extract is collected to obtain the schizonepeta inflorescence ethanol extract.

[0024] The preparation method of the ethanol extract of Nepeta stems comprises the following steps: After the Nepeta stems are dried, a second extraction is performed using 75% by volume ethanol as a second extraction solvent, and the extract is collected to obtain the Nepeta stem ethanol extract.

[0025] The material ratio of the Nepeta inflorescence to the second extraction solvent is 8 g:4.8 mL; the material ratio of the Nepeta stem to the second extraction solvent is 10 g:6 mL.

[0026] Preferably, the conditions for the second extraction are: extraction temperature 100° C., extraction pressure 10.34 MPa, and extraction time 5 min.

[0027] Compared with the prior art, the present invention has the following beneficial effects: 1. The purpose of the present invention is to develop a new strategy for the allelopathic potential of medicinal plants in regulating plant growth and development, and to provide an application of a schizonepeta extract in regulating the growth of crops, so as to expand the application of medicinal plants in more fields. The present invention has found that the schizonepeta inflorescence water extract, schizonepeta stem water extract, schizonepeta inflorescence water extract, schizonepeta stem water extract, schizonepeta inflorescence ethanol extract and schizonepeta stem ethanol extract have a significant inhibitory effect on lettuce seed germination and seedling growth, and a significant promoting effect on barley and green vegetable seed germination and seedling growth. The schizonepeta extract provided by the present invention can be used to regulate the growth of crops, thereby playing a role in regulating crop seed germination and / or seedling growth.

[0028] 2. The present invention has found that the aerial parts of Nepeta, Nepeta inflorescence water extract, Nepeta stem water extract, Nepeta inflorescence water extract, Nepeta stem water extract, Nepeta inflorescence ethanol extract and Nepeta stem ethanol extract have significant inhibitory effects on lettuce seed germination and seedling growth, and have significant promoting effects on barley and green vegetables seed germination and seedling growth by studying the allelopathic effect of Nepeta. Moreover, the inhibitory effect of the volatile substances in the Nepeta stem on lettuce is stronger than that of the Nepeta inflorescence, indicating that the volatile substances with inhibitory effects have a higher content in the Nepeta stem. On the contrary, the promoting effect of the volatile substances in the Nepeta inflorescence on barley and green vegetables is stronger than that of the Nepeta stem, indicating that the volatile substances with promoting effects have a higher content in the inflorescence.

[0029] 3. The inhibitory effect of the water extract of Nepeta inflorescence on lettuce is stronger than that of the water extract of Nepeta stem, indicating that the volatile substances with inhibitory effect are higher in the Nepeta inflorescence. Similarly, the promoting effect of the water extract of Nepeta inflorescence on barley and green vegetables is stronger than that of the water extract of Nepeta stem, indicating that the volatile substances with promoting effect are higher in the Nepeta inflorescence.

[0030] The inhibitory effect of Nepeta stem water extract and Nepeta stem ethanol extract on lettuce is stronger than that of Nepeta inflorescence water extract and Nepeta inflorescence ethanol extract, and the promoting effect on barley and green vegetables is also stronger than that of Nepeta stem, which indicates that the content of inhibitory volatile substances and promoting volatile substances is higher in Nepeta stem.

[0031] Therefore, among vegetables and crops, using appropriate concentrations of Nepeta water extract or water extract or ethanol extract to inhibit weeds around the desired vegetables and crops will also promote the growth of the plants. It can replace the use of chemical reagents while avoiding the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

[0032] The experimental results show that Nepeta has a strong allelopathic potential against weeds. Once there are more Nepeta plants in an environmental protection area with many plant species, there is a chance of causing serious damage. For example, the growth of plants in the protected area is affected and cannot grow healthily or directly die, resulting in fewer plant species and loss of balance in the ecological environment. Therefore, Nepeta should be discovered and cleared in time during management and maintenance. In addition, when it is necessary to remove certain weeds in agriculture, the appropriate use of Nepeta extracts or plant debris may have a certain removal effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is the effect of the sandwich method Nepeta inflorescence on the seed growth rate of the recipient plant; wherein, "R" represents the radicle and "H" represents the hypocotyl.

[0034] Figure 2 This is the effect of the sandwich method of Nepeta stems on the growth rate of recipient plant seeds; wherein, "R" represents the radicle and "H" represents the hypocotyl.

[0035] Figure 3 The effects of the water extract of Nepeta inflorescence on the growth of lettuce seedlings at different concentrations are expressed as growth rate, and its unit is %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0036] Figure 4 The effects of the aqueous extract of the stems of Nepeta tenuifolia at different concentrations on the growth of lettuce seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0037] Figure 5 The effects of the aqueous extract of the aerial parts of Nepeta tenuifolia at different concentrations on the growth of lettuce seedlings are expressed as growth rate, and the unit is %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0038] Figure 6 The present invention compares the effects of water extracts of various parts of Nepeta on the radicle growth rate of lettuce seedlings at different concentrations; wherein the water extracts of various parts of Nepeta include water extracts of Nepeta stems, water extracts of Nepeta inflorescences and water extracts of Nepeta aerial parts.

[0039] Figure 7 The effects of the water extract of Schizonepeta inflorescence on the growth of barley seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents radicle and "H" represents hypocotyl.

[0040] Figure 8 The effects of the aqueous extract of the stems of Nepeta tenuifolia at different concentrations on the growth of barley seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0041] Fig. 9 The effects of the water extract of Schizonepeta inflorescence on the growth of Brassica juncea seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0042] Fig.10 The effects of the aqueous extract of the stem of Nepeta tenuifolia at different concentrations on the growth of Brassica juncea seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0043] Fig.11 The effects of the ethanol extract of Nepeta inflorescence on the growth of lettuce seedlings at different concentrations are expressed as growth rate, and the unit is %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0044] Fig.12 The effects of the ethanol extract of Nepeta stems at different concentrations on the growth of lettuce seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0045] Fig.13 The effects of the ethanol extract of Nepeta inflorescence on the growth of barley seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents radicle and "H" represents hypocotyl.

[0046] Fig.14 The effects of the ethanol extract of Nepeta stems at different concentrations on the growth of barley seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0047] Fig.15 The effects of the ethanol extract of Nepeta inflorescence on the growth of Brassica juncea seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0048] Fig.16 The effects of the ethanol extract of Nepeta stems at different concentrations on the growth of Brassica juncea seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0049] Fig.17 The effects of the aqueous extract of Nepeta inflorescence on the growth of lettuce seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0050] Fig.18The effects of the aqueous extract of Nepeta stems at different concentrations on the growth of lettuce seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0051] Fig.19 The effects of the aqueous extract of Nepeta inflorescence on the growth of barley seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0052] Fig. 20 The effects of the aqueous extract of Nepeta stems at different concentrations on the growth of barley seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0053] Fig.21 The effects of the aqueous extract of Nepeta inflorescence on the growth of Brassica juncea seedlings at different concentrations are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl.

[0054] Fig. 22 The effects of the aqueous extract of the stems of Nepeta tenuifolia at different concentrations on the growth of Brassica juncea seedlings are expressed as growth rate, with the unit being %; wherein "R" represents the radicle and "H" represents the hypocotyl. DETAILED DESCRIPTION

[0055] The present invention is described in detail below in conjunction with the accompanying drawings and specific examples, but should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0056] The samples of Nepeta tenuifolia of the present invention were collected by natural collection and purchase. The first time was in July 2022, collected in Hanshan Nature Reserve, Zhalute Banner, Tongliao City, and the second time was purchased from Inner Mongolia Muxin Pharmaceutical Co., Ltd.

[0057] Recipient plants: lettuce, barley and greens.

[0058] Among them, lettuce ( Lactuca sativa var. ramosa Hort .) seeds were purchased from Hebei Qingfeng Seed Company, barley ( Hordeum vulgare L.) seeds were purchased from Liaodong Granary in Henan Province, and the greens were Shanghai greens ( Brassica rapa var chinensis (L.) Kitam.) seeds were purchased from Qingxian Xingyun Seed Co., Ltd.

[0059] The collected Nepeta samples were processed as follows: First, dry the specimens in a drying oven at 60°C for 4 hours. Then seal the specimen bags and store them in a dark place.

[0060] Example 1 1. Methods 1. Sandwich method The sandwich method was used to determine the effects of various parts of Nepeta on seed germination of three receptor plants. Among them, the various parts of Nepeta include Nepeta stems and Nepeta inflorescences. The three receptor plants include lettuce, barley, and green vegetables. Determination of the effects of various parts of Nepeta on seed germination of three receptor plants The effect of water from various parts of Nepeta on the radicle and hypocotyl of the three receptor plants was determined.

[0061] The sandwich method was developed based on the effects of active substances from tree fallen leaves and plant residues. In the previous research of the present invention, it was found that the average amount of fallen leaves from general trees is calculated to be 3t / 10000m per year. 2 According to the calculation of previous studies, 3t is used as the standard according to the different plant growth environments and regions. The collected Nepeta samples were dried in a 60℃ drying oven for 4 hours. Among them, the Nepeta samples are Nepeta stems and Nepeta inflorescences.

[0062] Nutrient agar was mixed with distilled water in a ratio of 15 mg: 2 mL, that is, 2 mL of distilled water and 15 mg of nutrient agar, and weighed to obtain nutrient agar medium. The nutrient agar medium was placed in a sterilizer and sterilized at 115 ° C for 15 min. A 6-well culture dish was used to weigh 10 mg and 50 mg of the treated Nepeta samples, 10 mg for 3 holes and 50 mg for 3 holes, and put them into a 6-well culture dish. 5 mL of the above nutrient agar was put into each hole, and 5 mL of nutrient agar was put into each hole after the nutrient agar solidified. After the nutrient agar solidified, seeds of three kinds of receptor plants were planted, and 5 seeds were planted in each hole, totaling: 6 holes × 5 seeds = 30 seeds. They were embedded in the nutrient agar medium in the form of a sandwich. At the same time, a 6-well culture dish without receptor plants was set up as a control group under the same conditions for the experiment. The three receptor plants germinated and grew. The six-well culture dish was sealed, wrapped with tin foil, and placed in a constant temperature incubator at 25°C for 3 days. The radicle length and hypocotyl length of the seeds of the three recipient plants were measured to infer the biological activities of the three recipient plants.

[0063] Among them, the processed Nepeta samples are the dried Nepeta stems and the dried Nepeta inflorescences.

[0064] 6-well culture dish: Multidish 6 Wells, Nunclon Delta Si, Thermo Fisher Scientific, China.

[0065] 3 receptor plants: lettuce, barley and greens.

[0066] The English name for the length of the radicle is Radicle. The English name for the length of the hypocotyl is Hypocotyl.

[0067] It should be noted that the reason why the present invention uses three kinds of recipient plants as donor plants and animals is that lettuce has high sensitivity and good reproducibility and represents dicotyledonous weeds. Among the chemical substances produced by plants, the sites where inhibitory activity is produced are different due to different substances. For example, some substances inhibit the radicle of the seedlings of the recipient plants, and some substances inhibit the growth of the hypocotyl. In order to grasp the inhibitory activity and to ensure that the two aspects of verification can be confirmed accurately, the length of the radicle and the length of the hypocotyl of the seedlings were measured.

[0068] 2. Preparation of extract (1) After the collected Nepeta stems and Nepeta inflorescences are placed in a drying box for drying, the dried Nepeta stems and the dried Nepeta inflorescences are mixed in a mass ratio of 1:1 to obtain a mixture of Nepeta stems and Nepeta inflorescences. The mixture of Nepeta stems and Nepeta inflorescences is referred to as the aerial part of Nepeta.

[0069] The aerial part of Nepeta is crushed, passed through a 20-mesh sieve, and then distilled water is added for extraction at a mass volume ratio of 8g / 100mL for aerial part of Nepeta to distilled water. The extraction is performed at 4°C in a refrigerator for 48 hours, and then filtered with two layers of gauze and qualitative filter paper. The obtained solution is the original solution of aerial part of Nepeta extract, which is sealed with a sealing film and stored in a refrigerator at 4°C for later use. The original solution of aerial part of Nepeta extract is called aerial part of Nepeta water extract.

[0070] (2) After the collected Nepeta inflorescences are placed in a drying oven for drying, the dried Nepeta inflorescences are crushed, passed through a 20-mesh sieve, and then extracted with distilled water at a mass volume ratio of 8 g / 100 mL of Nepeta inflorescence to distilled water. The extraction is performed in a refrigerator at 4°C for 48 hours, and then filtered with two layers of gauze and qualitative filter paper. The obtained solution is the Nepeta inflorescence extract stock solution, which is sealed with a sealing film and stored in a refrigerator at 4°C for future use. Among them, the Nepeta inflorescence extract stock solution is called Nepeta inflorescence water extract.

[0071] (3) After the collected Nepeta stems were placed in a drying oven to dry, the dried Nepeta stems were crushed, passed through a 20-mesh sieve, and then extracted with distilled water at a mass volume ratio of 8 g / 100 mL of Nepeta stems to distilled water. The extraction was performed in a refrigerator at 4°C for 48 hours, and then filtered with two layers of gauze and qualitative filter paper. The obtained solution was the Nepeta stem extract stock solution, which was sealed with a sealing film and stored in a refrigerator at 4°C for future use. The Nepeta stem extract stock solution is called Nepeta stem water extract.

[0072] 3. Preparation of water extract and ethanol extract (1) Using the Labtech fully automatic high-efficiency rapid solvent extractor, weigh 10 g of dried Nepeta stems and put them into an extraction tank. Use distilled water as the first extraction solvent and perform the first extraction according to the set procedure: extraction temperature 100°C, extraction pressure 10.34 MPa, extraction time 4 min, flushing volume 60% of the sample volume, 2 cycles, and nitrogen purge time 30 sec. After the extraction, collect the extract to obtain Nepeta stem water extract. The material ratio of Nepeta stem to the first extraction solvent is 10 g: 6 mL.

[0073] (2) Using the Labtech fully automatic high-efficiency rapid solvent extractor, weigh 5 g of dried Nepeta inflorescence and put it into the extraction tank. Use distilled water as the first extraction solvent and perform the first extraction according to the set procedure: extraction temperature 100°C, extraction pressure 10.34 MPa, extraction time 4 min, flushing volume 60% of the sample volume, 2 cycles, nitrogen purge time 30 sec. After the extraction, collect the extract to obtain the Nepeta inflorescence water extract. The material ratio of Nepeta inflorescence to the first extraction solvent is 5 g:3 mL.

[0074] (3) Weigh 10 g of dried Nepeta stems, use 75% ethanol by volume as the second extraction solvent, and perform a second extraction according to the set procedure: extraction temperature 100°C, extraction pressure 10.34 MPa, extraction time 5 min, flushing volume 60% of the sample volume, 2 cycles, nitrogen purge time 30 sec. After the extraction, collect the extract to obtain Nepeta stem ethanol extract. The material ratio of Nepeta stem to the second extraction solvent is 10 g: 6 mL.

[0075] (4) Weigh 8 g of dried Nepeta inflorescence, use 75% ethanol by volume as the second extraction solvent, and perform a second extraction according to the set procedure: extraction temperature 100°C, extraction pressure 10.34 MPa, extraction time 5 min, flushing volume 60% of the sample volume, 2 cycles, nitrogen purge time 30 sec. After the extraction, collect the extract to obtain Nepeta inflorescence ethanol extract. The material ratio of Nepeta inflorescence to the second extraction solvent is 8 g:4.8 mL.

[0076] The extracts obtained above were placed into solvent storage bottles, sealed with sealing film, and placed in a refrigerator for later use.

[0077] Among them, the English abbreviation of LabTech is Lab Tech.

[0078] Fully automatic high-efficiency and fast solvent extractor: Flex-HPSE.

[0079] 4. Growth inhibition assay, EC 50 The growth inhibition experiment was an experiment to measure the effects of extracts from Nepeta stems and Nepeta inflorescences on the growth of young roots and hypocotyls of three receptor plants.

[0080] Among them, the full English name of EC 50 is Effective Concentration 50%.

[0081] The three receptor plants are lettuce, barley and Chinese cabbage, among which Chinese cabbage is Shanghai green.

[0082] The extracts of Nepeta stem and Nepeta inflorescence in this part of the experiment include aqueous extract of Nepeta aerial part, aqueous extract of Nepeta stem, aqueous extract of Nepeta inflorescence, aqueous extract of Nepeta stem, aqueous extract of Nepeta inflorescence, ethanol extract of Nepeta stem and ethanol extract of Nepeta inflorescence.

[0083] (1) For the first time, the aqueous extracts of aerial parts of Nepeta tenuifolia, inflorescences of Nepeta tenuifolia and stems of Nepeta tenuifolia prepared above were diluted to the following gradient concentrations: 0.04 g / mL, 0.02 g / mL, 0.01 g / mL, 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, 0.002 g / mL, 0.001 g / mL and 0.0005 g / mL, respectively, for growth inhibition experiment.

[0084] (2) For the second time, 10 g of Nepeta stem was taken and 75% by volume ethanol was used as the second extraction solvent. The second extraction treatment was carried out according to the above method. The Nepeta stem extract was subjected to rotary evaporation to obtain an extract. Then, the obtained Nepeta stem extract of 74 mg was adjusted to the concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL and 0.001 g / mL in sequence to obtain Nepeta stem ethanol extracts of different concentrations for growth inhibition experiments.

[0085] (3) For the second time, 5 g of Nepeta inflorescence was taken, and 75% by volume ethanol was used as the second extraction solvent. The second extraction treatment was carried out according to the above method. The obtained Nepeta inflorescence extract was subjected to rotary evaporation to obtain an extract. Then, the obtained Nepeta inflorescence extract of 187 mg was adjusted to the concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL and 0.001 g / mL in sequence to obtain Nepeta inflorescence ethanol extracts of different concentrations, and the growth inhibition experiment was carried out.

[0086] (4) For the third time, 10 g of Nepeta stem was taken and distilled water was used as the first extraction solvent. The first extraction treatment was carried out according to the above method. The obtained extract was placed in an evaporating dish and placed in a constant temperature water bath at 100°C. It was left to stand for a while until it became an extract. Then, the obtained 542 mg Nepeta stem extract was adjusted to the concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL and 0.001 g / mL in sequence to obtain Nepeta stem water extracts of different concentrations for growth inhibition experiments.

[0087] (5) For the third time, 5 g of Schizonepeta inflorescence was taken and distilled water was used as the first extraction solvent. The first extraction treatment was carried out according to the above method. The obtained extract was placed in an evaporating dish and placed in a constant temperature water bath at 100°C. It was left to stand until it became an extract. Then, the obtained 415 mg of Schizonepeta inflorescence extract was adjusted to the concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL and 0.001 g / mL in sequence to obtain Schizonepeta inflorescence water extracts of different concentrations for growth inhibition experiments.

[0088] The specific operation of the growth inhibition experiment is as follows: Lettuce seeds, barley seeds, and Chinese cabbage seeds were placed in a 9 cm dish for germination for 24 hours. Filter paper was placed on a 33 mm culture dish, and 5 germinated lettuce seeds, barley seeds, and Chinese cabbage seeds were placed in each dish. 1 mL of each of the above-mentioned concentrations of water extract of the aerial part of Nepeta tenuifolia, water extract of Nepeta tenuifolia stem, water extract of Nepeta tenuifolia inflorescence, water extract of Nepeta tenuifolia stem, water extract of Nepeta tenuifolia inflorescence, ethanol extract of Nepeta tenuifolia stem, and ethanol extract of Nepeta tenuifolia inflorescence was placed in each culture dish, and grown in a 25°C constant temperature device for 2 days. Two days later, the length of the young root and the length of the hypocotyl of the lettuce seedlings in each culture dish were measured. The length of the radicle or the length of the hypocotyl of the seedlings was plotted against the elongation of each concentration, and the concentration that inhibited the growth by 50% was defined as EC 50.

[0089] Among them, the full name of EC 50 is Effective Concentration 50%.

[0090] 5. Data processing The measured data were statistically sorted using Microsoft Excel and then analyzed for data significance and regularity.

[0091] 2. Results 1. Sandwich assay results and analysis The research results are shown in Table 1 and Figure 1 shown.

[0092] Table 1 Effects of extracts from inflorescence and stem of Nepeta tenuifolia on seed germination of three receptor plants determined by sandwich method

[0093] Note: “R” stands for radicle and “H” stands for hypocotyl.

[0094] Table 1 and Figure 1 The results showed that in the sandwich method, when the concentration of 1 mg / mL and 5 mg / mL was used, the inflorescence of Nepeta showed a 100% inhibitory effect on the radicle and hypocotyl of lettuce seed seedlings. When barley seeds were used as the recipient plant, the inflorescence of Nepeta showed a weak inhibitory effect on the hypocotyl at the concentration of 1 mg / mL and 5 mg / mL, and showed a certain promoting effect on the radicle of the seedlings. When the concentration of 1 mg / mL was used as the recipient plant, the inflorescence of Nepeta showed a certain inhibitory effect on the radicle and hypocotyl of the seedlings, and showed a certain promoting effect on the radicle and hypocotyl of the seedlings at the concentration of 5 mg / mL. Among them, the concentration of Nepeta inflorescence in the concentrations of 1 mg / mL and 5 mg / mL refers to the ratio of the mass of the dried Nepeta inflorescence to the volume of nutrient agar.

[0095] Table 1 and Figure 2 The results showed that at the concentration of 1mg / mL and 5mg / mL, Nepeta stems showed a strong inhibitory effect on the radicle and hypocotyl of lettuce seed seedlings. When barley seeds were used as the recipient plant, Nepeta stems showed a weak inhibitory effect on the radicle and hypocotyl of seedlings at the concentration of 1mg / mL and 5mg / mL. When Chinese cabbage seeds were used as the recipient plant, Nepeta stems showed a weak promoting effect on the radicle and hypocotyl of seedlings at the concentration of 1mg / mL and 5mg / mL. Among them, the concentration of Nepeta stems in the concentrations of 1mg / mL and 5mg / mL refers to the ratio of the mass of Nepeta stems after drying to the volume of nutrient agar.

[0096] 2. Results and analysis of growth inhibition experiments 2.1 Water extract Table 2 and Figure 3The results showed that in the growth inhibition experiment, with lettuce as the receptor plant, the water extract of Schizonepeta inflorescence showed strong inhibitory effects on the radicle and hypocotyl of seedlings at concentrations of 0.08g / mL, 0.04g / mL and 0.02g / mL; the water extract of Schizonepeta inflorescence showed a certain inhibitory effect on the radicle of seedlings at concentrations of 0.01g / mL, 0.008g / mL, 0.006g / mL, 0.004g / mL, 0.003g / mL and 0.002g / mL, and the inhibitory effect gradually became stronger with the increase of concentration; the water extract of Schizonepeta inflorescence showed a certain promoting effect on the hypocotyl at concentrations of 0.008g / mL, 0.006g / mL, 0.004g / mL, 0.003g / mL and 0.002g / mL, and the promoting effect gradually became stronger with the decrease of concentration.

[0097] Table 2 and Figure 4 The results showed that in the growth inhibition experiment, with lettuce as the receptor plant, the water extract of Nepeta stem showed 100% inhibition effect on the radicle and hypocotyl of seedlings at a concentration of 0.08g / mL; the water extract of Nepeta stem showed a certain inhibition effect on the radicle of seedlings at concentrations of 0.04g / mL, 0.02g / mL, 0.01g / mL, 0.008g / mL, 0.006g / mL, 0.004g / mL, 0.003g / mL and 0.002g / mL, and the inhibition effect gradually increased with the increase of concentration; the water extract of Nepeta stem showed a certain promotion effect on the hypocotyl at concentrations of 0.008g / mL, 0.006g / mL, 0.004g / mL, 0.003g / mL and 0.002g / mL, and the promotion effect gradually increased with the decrease of concentration.

[0098] Table 2 and Figure 5 The results showed that in the growth inhibition experiment, with lettuce as the receptor plant, the water extract of the aerial parts of Nepeta showed 100% inhibition effect on the radicle and hypocotyl of seedlings at concentrations of 0.08g / mL, 0.04g / mL and 0.02g / mL; the water extract of the aerial parts of Nepeta showed a certain inhibition effect on the radicle of seedlings at concentrations of 0.01g / mL, 0.008g / mL, 0.006g / mL, 0.004g / mL, 0.003g / mL and 0.002g / mL, and the inhibition effect gradually increased with the increase of concentration; the water extract of the aerial parts of Nepeta showed a certain promotion effect on the hypocotyl at concentrations of 0.008g / mL, 0.006g / mL, 0.004g / mL, 0.003g / mL and 0.002g / mL, and the promotion effect gradually increased with the decrease of concentration.

[0099] Figure 6As shown in the results, in the growth inhibition experiment with lettuce as the receptor plant, if the results of the water extracts of the aboveground parts of Nepeta, the water extracts of the stems of Nepeta and the water extracts of the inflorescences of Nepeta are compared, the inhibitory effects of the water extracts of the aboveground parts of Nepeta and the water extracts of the inflorescences of Nepeta are stronger than those of the stems of Nepeta, which also shows that the allelopathic substances in the inflorescence of Nepeta are stronger than those in other parts.

[0100] Table 2 Effects of water extracts from various parts of Nepeta tenuifolia at different concentrations on the growth of lettuce seedlings

[0101] Note: “R” stands for radicle and “H” stands for hypocotyl.

[0102] Table 3 and Figure 7 The results showed that in the growth inhibition experiment, with barley as the receptor plant, the water extract of Schizonepeta inflorescence showed a certain inhibitory effect on the radicle and hypocotyl of seedlings at concentrations of 0.0030g / mL, 0.0028g / mL, 0.0026g / mL, 0.0024g / mL, 0.0020g / mL, 0.0018g / mL, 0.0016g / mL and 0.0014g / mL, and the inhibitory effect gradually strengthened with the increase of concentration; the water extract of Schizonepeta inflorescence showed a certain promoting effect on the radicle and hypocotyl of seedlings at concentrations of 0.0010g / mL, 0.0008g / mL, 0.0006g / mL and 0.0004g / mL, and the promoting effect gradually strengthened with the decrease of concentration.

[0103] Table 3 and Figure 8 The results showed that in the growth inhibition experiment, with barley as the recipient plant, the water extract of Nepeta stems showed weak inhibitory effects on the radicle and hypocotyl of seedlings at concentrations of 0.0030g / mL, 0.0028g / mL, 0.0026g / mL, 0.0024g / mL, 0.0020g / mL, 0.0018g / mL, 0.0016g / mL and 0.0014g / mL; the water extract of Nepeta stems showed weak inhibitory effects on the hypocotyls, but strong promoting effects on the radicle of seedlings at concentrations of 0.0010g / mL, 0.0008g / mL, 0.0006g / mL and 0.0004g / mL.

[0104] Table 3 Effects of water extracts of Nepeta inflorescence and Nepeta stem on the growth of barley seedlings at different concentrations

[0105] Note: “R” stands for radicle and “H” stands for hypocotyl.

[0106] Table 4 and Fig. 9The results showed that in the growth inhibition experiment with Chinese cabbage as the recipient plant, the water extract of Schizonepeta inflorescence showed a certain inhibitory effect on the radicle of seedlings at the concentrations of 0.0030g / mL and 0.0028g / mL, and a promoting effect on the hypocotyl; the water extract of Schizonepeta inflorescence showed a certain inhibitory effect on the radicle and hypocotyl of seedlings at the concentrations of 0.0026g / mL and 0.0024g / mL; the water extract of Schizonepeta inflorescence showed a strong inhibitory effect on the radicle of seedlings at the concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL, 0.0010g / mL and 0.0008g / mL, 0.0006g / mL and 0.0004g / mL.

[0107] Table 4 and Fig.10 The results showed that in the growth inhibition experiment, with green vegetables as the recipient plant, the water extract of Nepeta stems showed a certain inhibitory effect on the radicle and hypocotyl of seedlings at concentrations of 0.0030g / mL, 0.0028g / mL, 0.0026g / mL, 0.0024g / mL, 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL, 0.0010g / mL, 0.0008g / mL, 0.0006g / mL and 0.0004g / mL, and the effect was very unstable. Except for the concentrations of 0.0026g / mL and 0.0024g / mL, the rest showed a certain promoting effect on the hypocotyl.

[0108] Table 4 Effects of water extracts of Nepeta inflorescence and Nepeta stem on the growth of Brassica juncea seedlings at different concentrations

[0109] Note: “R” stands for radicle and “H” stands for hypocotyl.

[0110] 2.2 Ethanol extract Table 5 and Fig.11 The results showed that in the growth inhibition experiment, with lettuce as the recipient plant, the ethanol extract of Nepeta inflorescence showed a weak inhibitory effect on the radicle and hypocotyl of seedlings at concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL.

[0111] Table 5 and Fig.12The results showed that in the growth inhibition experiment, with lettuce as the recipient plant, the ethanol extract of Nepeta stems showed strong inhibitory effects on the radicle and hypocotyl of lettuce seedlings at concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL, and the inhibitory effect tended to become stronger with the increase of concentration.

[0112] Table 5 Fig.13 and Fig.14 The results showed that in the growth inhibition experiment, with barley as the receptor plant, the ethanol extracts of nepeta inflorescence and nepeta stem showed certain inhibitory effects on the radicle and hypocotyl of seedlings at concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL. Moreover, the inhibitory effect of the ethanol extract of nepeta inflorescence was stronger than that of the ethanol extract of nepeta stem.

[0113] Table 5 and Fig.15 The results showed that in the growth inhibition experiment with green vegetables as the recipient plant, the ethanol extract of Nepeta inflorescence showed a weak inhibitory effect on the radicle and hypocotyl of seedlings at a concentration of 0.0020g / mL; the ethanol extract of Nepeta inflorescence showed a promoting effect on the radicle and hypocotyl of seedlings at concentrations of 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL, and the promoting effect tended to gradually increase with the decrease in concentration.

[0114] Table 5 and Fig.16 The results showed that in the growth inhibition experiment with P. napus as the recipient plant, the ethanol extract of Nepeta tenuifolia stems showed a certain promoting effect on the radicle and hypocotyl of seedlings at concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL and 0.0012 g / mL.

[0115] Table 5 Effects of ethanol extracts of Nepeta inflorescence and Nepeta stem at different concentrations on the growth of recipient plant seedlings

[0116] Note: “R” stands for radicle and “H” stands for hypocotyl.

[0117] 2.3 Water Extract Table 6 Fig.17 and Fig.18The results showed that in the growth inhibition experiment, when lettuce was used as the receptor plant, the water extract of schizonepeta inflorescence showed a strong inhibitory effect on the radicle of lettuce seedlings at the concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL, and showed a certain inhibitory effect on the hypocotyl of lettuce. Compared with the water extract of schizonepeta stem and the water extract of schizonepeta inflorescence, the inhibitory effect of the water extract of schizonepeta inflorescence on lettuce was stronger than that of the water extract of schizonepeta stem.

[0118] Table 6 Fig.19 and Fig. 20 The results showed that in the growth inhibition experiment, with barley as the receptor plant, the water extracts of nepeta inflorescence and nepeta stems showed certain promoting effects on the radicle and hypocotyl of barley seedlings at concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL. Comparing the water extracts of nepeta inflorescence and nepeta stems, the promoting effect of nepeta stems on barley was stronger than that of nepeta inflorescence.

[0119] Table 6 Fig.21 and Fig. 22 The results showed that in the growth inhibition experiment, with P. napus as the recipient plant, the water extract of Nepeta inflorescence and the water extract of Nepeta stem showed a strong promoting effect on the radicle of P. napus seedlings and the hypocotyl of P. napus seedlings at concentrations of 0.0020g / mL, 0.0018g / mL, 0.0016g / mL, 0.0014g / mL and 0.0012g / mL.

[0120] Table 6 Effects of water extracts of Nepeta inflorescence and Nepeta stem on the growth of recipient plant seedlings at different concentrations

[0121] Note: “R” stands for radicle and “H” stands for hypocotyl.

[0122] In the sandwich assay, nepeta inflorescence and nepeta stem showed 100% inhibitory effect on the growth of lettuce seedlings at 1mg / mL and 5mg / mL concentrations. The EC 50 method was used to determine the strength of the inhibitory effect at different concentrations. Among them, the water extract of nepeta inflorescence and the water extract of nepeta stem showed inhibitory effects at concentrations of 0.002g / mL to 0.08g / mL, and the inhibitory effect gradually became stronger with the increase of concentration. The water extract of nepeta inflorescence and the water extract of nepeta stem showed a weak inhibitory effect on lettuce at different concentrations, and the inhibitory effect also tended to be super strong with the increase of concentration. Nepeta inflorescence and nepeta stem showed a promoting effect on barley and green vegetables as a whole. The EC50 method determined that the promoting effect gradually became stronger with the decrease of concentration. The ethanol extract of nepeta inflorescence and the ethanol extract of nepeta stem showed a weak inhibitory effect on the growth of barley seedlings at different concentrations, and showed a promoting effect on the growth of green vegetable seedlings.

[0123] The research result of the present invention is that the aerial parts of Nepeta, the water extract of Nepeta inflorescence and the water extract of Nepeta stem, the water extract of Nepeta inflorescence and the water extract of Nepeta stem, and the ethanol extract of Nepeta inflorescence and the ethanol extract of Nepeta stem have a significant inhibitory effect on the seed germination and seedling growth of lettuce, and have a significant promoting effect on the seed germination and seedling growth of barley and green vegetables. Moreover, the inhibitory effect of the volatile substances in the Nepeta stem on lettuce is stronger than that in the Nepeta inflorescence, indicating that the volatile substances with inhibitory effect are higher in the Nepeta stem. On the contrary, the promoting effect of the volatile substances in the Nepeta inflorescence on barley and green vegetables is stronger than that in the Nepeta stem, which indicates that the volatile substances with promoting effect are higher in the Nepeta inflorescence.

[0124] The inhibitory effect of the water extract of Nepeta inflorescence on lettuce is stronger than that of the water extract of Nepeta stem, indicating that the content of volatile substances with inhibitory effect is higher in Nepeta inflorescence. Similarly, the promoting effect of the water extract of Nepeta inflorescence on barley and green vegetables is stronger than that of the water extract of Nepeta stem, indicating that the content of volatile substances with promoting effect is higher in Nepeta inflorescence.

[0125] The inhibitory effect of Nepeta stem water extract and Nepeta stem ethanol extract on lettuce is stronger than that of Nepeta inflorescence water extract and Nepeta inflorescence ethanol extract, and the promoting effect on barley and green vegetables is also stronger than that of Nepeta stem water extract and Nepeta stem ethanol extract, which indicates that the content of inhibitory volatile substances and promoting volatile substances is higher in Nepeta stem.

[0126] Therefore, among vegetables and crops, using appropriate concentrations of Nepeta extract or extract to inhibit weeds around the desired vegetables and crops will also promote the growth of the plants. It can replace the use of chemical reagents while avoiding the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

[0127] At the same time, the present invention also found through experiments that Nepeta has a strong allelopathic potential on weeds. Once there are more Nepeta plants in an environmental protection area with many plant species, there is a chance of causing more serious harm, such as the growth of plants in the protection area being affected and unable to grow healthily or directly dying, thereby causing fewer plant species and loss of balance in the ecological environment. Therefore, Nepeta should be discovered and cleared in time during management and maintenance. In addition, when it is necessary to remove certain weeds in agriculture, Nepeta extracts or plant debris can be appropriately used, which may have a certain removal effect.

[0128] Therefore, among vegetables and crops, using appropriate concentrations of Nepeta extract or extract to inhibit weeds around the desired vegetables and crops will also promote the growth of the plants. It can replace the use of chemical reagents while avoiding the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

[0129] The experimental results show that Nepeta has a strong allelopathic potential against weeds. Once there are more Nepeta plants in an environmental protection area with many plant species, there is a chance of causing serious damage. For example, the growth of plants in the protected area is affected and cannot grow healthily or directly die, resulting in fewer plant species and loss of balance in the ecological environment. Therefore, Nepeta should be discovered and cleared in time during management and maintenance. In addition, when it is necessary to remove certain weeds in agriculture, the appropriate use of Nepeta extracts or plant debris may have a certain removal effect.

[0130] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. In order to avoid redundancy, the present invention describes a preferred embodiment.

[0131] Although preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they understand the basic inventive concepts, and all such changes and modifications fall within the scope of the present invention.

Claims

1. Application of Nepeta extract in regulating the growth of crops, characterized in that: The nepeta extract regulates the growth of the crop through the allelopathic effect on the crop; the nepeta extract includes nepeta inflorescence extract and / or nepeta stem extract; The nepeta inflorescence extract comprises at least one of a nepeta inflorescence water extract, a nepeta inflorescence water extract and a nepeta inflorescence ethanol extract; The Nepeta stem extract comprises at least one of Nepeta stem water extract, Nepeta stem water extract and Nepeta stem ethanol extract.

2. The use of the Nepeta extract in regulating the growth of crops according to claim 1, characterized in that: The crops include any one or more of lettuce, barley and green vegetables.

3. The use of the Nepeta extract in regulating the growth of crops according to claim 1, characterized in that: The nepeta extract is used for regulating the seed germination and seedling growth of the crops.

4. The use of the Nepeta extract in regulating the growth of crops according to claim 3, characterized in that: The regulation includes promotion and / or inhibition.

5. The use of the Nepeta extract in regulating the growth of crops according to claim 1, characterized in that: The water extract of Nepeta inflorescence is obtained by adding water to extract the Nepeta inflorescence at 4°C, filtering and collecting the filtrate; The water extract of Nepeta stem is obtained by adding water to the Nepeta stem at 4°C, extracting it, and filtering the collected filtrate; The material ratio of the schizonepeta inflorescence to the water and the material ratio of the schizonepeta stem to the water are both 8g:100mL.

6. The use of the Nepeta extract in regulating the growth of crops according to claim 1, characterized in that: The schizonepeta inflorescence water extract is obtained by using the schizonepeta inflorescence as a raw material and water as a first extraction solvent to perform a first extraction and collect the extract; The Nepeta stem water extract is obtained by using the Nepeta stem as a raw material and water as a first extraction solvent to perform a first extraction and collect the extract; The material ratio of the Nepeta inflorescence to the first extraction solvent is 5 g:3 mL; the material ratio of the Nepeta stem to the first extraction solvent is 10 g:6 mL.

7. The use of the Nepeta extract in regulating the growth of crops according to claim 6, characterized in that: The conditions for the first extraction were: extraction temperature 100° C., extraction pressure 10.34 MPa, and extraction time 4 min.

8. The use of the Nepeta extract in regulating the growth of crops according to claim 1, characterized in that: The ethanol extract of Nepeta inflorescence is prepared by using the Nepeta inflorescence as a raw material and using 75% ethanol by volume as a second extraction solvent to perform a second extraction and collect the extract; The ethanol extract of Nepeta stem is prepared by using the Nepeta stem as a raw material and using 75% ethanol by volume as a second extraction solvent to perform a second extraction and collect the extract; The material ratio of the Nepeta inflorescence to the second extraction solvent is 8 g:4.8 mL; the material ratio of the Nepeta stem to the second extraction solvent is 10 g:6 mL.

9. The use of the Nepeta extract in regulating the growth of crops according to claim 8, characterized in that: The conditions for the second extraction were: extraction temperature 100° C., extraction pressure 10.34 MPa, and extraction time 5 min.

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