A growth regulator and its application in the cultivation of Brachiosa multifiliis.

Abstract

This invention discloses a growth regulator and its application in the cultivation of *Bombyx mori*. The growth regulator comprises: 1-3 parts lentinan, 4-8 parts taurine, 5-10 parts lysine, 2-4 parts ethyl m-aminobenzoate methanesulfonate, and 10-15 parts *Eucommia ulmoides* leaf extract. The growth regulator significantly improves the survival rate and weight of *Bombyx mori*. Furthermore, when applied to sand-free cultivation of *Bombyx mori*, the growth regulator eliminates the need to treat contaminated sand layers, saving labor and material costs and further enhancing the economic benefits of cultivation. Sand-free cultivation also facilitates observation of the growth and health status of *Bombyx mori*, enabling timely detection of problems and implementation of appropriate measures. It simplifies the cultivation process, making management more convenient and efficient, and contributing to improved cultivation efficiency.

Description

Technical Field

[0001] This invention belongs to the technical field of snail cultivation, and more specifically, relates to a growth regulator and its application in the cultivation of Oriental Wind Snails. Background Technology

[0002] The spotted whelk (Babylonia areolata), commonly known as the flower whelk, belongs to the class Gastropoda, order Acanthoglossi, family Mothidae, and genus Babylonia. It is a carnivorous mollusc that lives in muddy or sandy bottoms at depths of several to tens of meters in tropical and subtropical regions. The spotted whelk grows quickly, has a short cultivation cycle, and its meat is crisp, delicious, and possesses extremely high nutritional and medicinal value. However, as a newly cultivated species, its cultivation techniques and nutritional requirements are still underdeveloped, and a series of problems remain, such as imperfect cultivation models, outdated technology, frequent outbreaks of diseases, and ineffective disease control measures.

[0003] Huang Ruipin et al. disclosed that the most common farming model for *Bambusa serrata* is outdoor cement pond with flowing water, combined with top shading and bottom sand covering. During the farming process, the large amount of mucus and uneaten food excrement secreted by *Bambusa serrata* can lead to organic matter accumulation and bacterial proliferation if not cleaned in time, ultimately resulting in bottom sediment deterioration. Furthermore, because *Bambusa serrata* has a tendency to burrow in sand, the deterioration of the bottom environment not only affects its growth and development but also increases the risk of disease. At the genus level, the dominant genus of bacteria in the sand is *Vibrio* (Huang Ruipin, Zhang Lei, Zhang Weixiao et al. Effects of ceramsite material and particle size on the survival, growth, and bottom microbial community of *Bambusa serrata* [J]. Journal of Dalian Ocean University).

[0004] Traditional aquaculture techniques have the following drawbacks: sand is placed in the aquaculture pond, there is uneaten feed during the feeding process, and the snails produce a lot of feces during the aquaculture process. This uneaten feed and feces mixed with the sand can easily breed a large number of harmful bacteria and pathogens, which will deteriorate the water quality, pollute the sand layer, cause it to turn black, and the number of harmful bacteria will exceed the standard, reducing the survival rate of snails. In severe cases, it may lead to infectious diseases and cause the entire snail population to perish, greatly increasing the aquaculture risk.

[0005] Emerging technologies also include sand-free aquaculture through water circulation, addressing the shortcomings of traditional sand-based aquaculture, such as the accumulation of pollutants in the sand substrate and the susceptibility to disease outbreaks. For example, Chinese patent application CN118844368A discloses a sand-free recirculating aquaculture system for *Bellamya aegypti* (a type of snail). This system designs a self-draining sand-free aquaculture pond and a lower-level water treatment system below it. The aquaculture wastewater from the self-draining sand-free aquaculture pond flows by gravity into the lower-level water treatment system for purification. The purified wastewater is then pumped back into the self-draining sand-free aquaculture pond, thus improving upon the problem of excessive pollutant accumulation in the sand substrate of traditional sand-based aquaculture. While the aforementioned existing technologies directly remove sand and use water circulation systems to promptly discharge uneaten feed and feces from the aquaculture pond, reducing water quality deterioration and sand layer pollution to some extent, *Bellamya aegypti* is a sand-burrowing organism. Directly removing sand during aquaculture forces a change in its hiding habits, causing severe stress and ultimately reducing the survival rate of *Bellamya aegypti*. Summary of the Invention

[0006] In view of the above-mentioned existing technical problems, the purpose of this invention is to provide a growth regulator.

[0007] Another object of the present invention is to provide a method for preparing the growth regulator.

[0008] Another object of the present invention is to provide the application of the growth regulator in the cultivation of *Bambusa multiplex*.

[0009] Another objective of this invention is to provide a method for sand-free cultivation of *Bambusa multiplex*. By employing sand-free cultivation technology, feces can be discharged in a timely manner, improving the living environment of *Bambusa multiplex* and increasing its survival rate.

[0010] To achieve the above objectives, the present invention is implemented through the following technical solution:

[0011] This invention provides a growth regulator comprising the following raw materials in parts by weight: 1-3 parts lentinan, 4-8 parts taurine, 5-10 parts lysine, 2-4 parts ethyl m-aminobenzoate methanesulfonate, and 10-15 parts Eucommia ulmoides leaf extract.

[0012] Lentinan is an immunomodulator. When the African whelk is protected from disease, it can activate the whelk's immune system and improve its survival rate. Lentinan regulates enzymes related to nutrient absorption and energy metabolism in the whelk, enabling it to absorb nutrients from food more efficiently, thus allocating more energy for growth and contributing to increased whelk weight.

[0013] Taurine plays a crucial role in the osmotic pressure regulation and ion balance of the *Bellamya aegypti* snail. Within the snail's cells, taurine helps maintain osmotic pressure balance, allowing the snail to better adapt to environmental changes, reducing mortality caused by environmental stress, and increasing survival rate. Taurine participates in the growth and development of the snail, acting as a regulator of certain substances related to growth hormone regulation. It can promote the normal secretion and function of growth hormone in the snail, thereby stimulating growth and increasing snail weight.

[0014] Lysine is an essential raw material for protein synthesis. In the body of the African whelk, sufficient lysine ensures the normal synthesis of various proteins, including muscle proteins and enzymes. Abundant muscle proteins contribute to the whelk's growth and increase its weight; while the normal synthesis of various enzymes ensures the smooth progress of physiological and biochemical reactions within the whelk, thus providing a good physiological foundation for its survival and growth, and improving its survival rate. Lysine also participates in the whelk's immune defense mechanism. It enhances the whelk's immunity by influencing the synthesis of immune proteins. When the whelk is attacked by pathogens, these immune proteins can identify and eliminate pathogens, reducing the occurrence of disease and thus improving the whelk's survival rate.

[0015] During aquaculture, *Bombyx mori* (a type of whelk) are subject to various stressors, such as drastic environmental changes and harvesting operations. Ethyl m-aminobenzoate methanesulfonate can help keep *Bombyx mori* in a relatively calm state, reducing energy consumption and potential physical damage caused by stress. It's like putting *Bombyx mori* into a "low-energy" state, thereby improving its survival rate under adverse environmental or operational conditions. Because stress responses are reduced, *Bombyx mori* can dedicate more energy to growth. When *Bombyx mori* can survive in a relatively stable state, its normal physiological activities, such as feeding and nutrient absorption, are not frequently interrupted, which is conducive to nutrient accumulation and increased whelk weight.

[0016] Eucommia ulmoides leaf extract contains various antioxidants that can protect the cells of the African whelk from oxidative damage, maintain cell integrity and normal function, thereby improving the survival rate of the whelk. Eucommia ulmoides leaf extract can also enhance the immunity of the African whelk. It contains components that can stimulate the immune system of the African whelk, activate immune cells, and enable the whelk to better resist disease. At the same time, Eucommia ulmoides leaf extract may promote the secretion of growth-related hormones in the African whelk or regulate its physiological metabolism, enabling the whelk to better utilize nutrients in its food, promote growth, and increase whelk weight.

[0017] Through research, the inventors discovered that the combined use of lentinan, taurine, lysine, ethyl m-aminobenzoate methanesulfonate, and Eucommia ulmoides leaf extract can alleviate the stress response that easily occurs during sand-free farming of Rosa rugosa, and significantly improve the survival rate and weight of Rosa rugosa.

[0018] Preferably, the growth regulator further contains at least one of the following ingredients in parts by weight: 1-2 parts vitamin E, 1-2 parts vitamin C, 6-15 parts potassium chloride, or 3-7 parts citric acid.

[0019] Further research by the inventors revealed that by combining lentinan, taurine, lysine, ethyl m-aminobenzoate methanesulfonate, and Eucommia ulmoides leaf extract, the addition of vitamin E, vitamin C, potassium chloride, or citric acid can further improve the survival rate and weight of the snails in the Dongfeng snail farm.

[0020] Preferably, the growth regulator comprises the following raw materials in parts by weight: 1-3 parts lentinan, 4-8 parts taurine, 5-10 parts lysine, 2-4 parts ethyl m-aminobenzoate methanesulfonate, 10-15 parts Eucommia ulmoides leaf extract, 1-2 parts vitamin E, and 1-2 parts vitamin C.

[0021] Preferably, the growth regulator comprises the following raw materials in parts by weight: 1-3 parts lentinan, 4-8 parts taurine, 5-10 parts lysine, 2-4 parts ethyl m-aminobenzoate methanesulfonate, 10-15 parts Eucommia ulmoides leaf extract, 6-15 parts potassium chloride, and 3-7 parts citric acid.

[0022] Preferably, the growth regulator comprises the following raw materials in parts by weight: 1-3 parts lentinan, 4-8 parts taurine, 5-10 parts lysine, 2-4 parts ethyl m-aminobenzoate methanesulfonate, 10-15 parts Eucommia ulmoides leaf extract, 1-2 parts vitamin E, 1-2 parts vitamin C, 6-15 parts potassium chloride, and 3-7 parts citric acid.

[0023] The present invention also provides a method for preparing the growth regulator, wherein the raw materials are mixed in parts by weight to obtain the growth regulator.

[0024] In addition, the present invention protects the application of the growth regulator in the cultivation of African whelks.

[0025] More specifically, the *Bellamya aegyptiaca* farming is either sand-free farming or sand-containing farming. Preferably, the *Bellamya aegyptiaca* farming is sand-free farming.

[0026] Furthermore, the present invention provides a method for sand-free culture of *Sinocyclocheilus edulis*, wherein the growth regulator described in the present invention is added to the culture pond and no sand is laid in the culture pond.

[0027] This invention provides a sand-free aquaculture method. By adding the growth regulator described in this invention during the aquaculture process, the method avoids the problem of harmful bacteria and pathogens breeding in the sand mixed with uneaten feed and feces, which is common in sand-cultured snails. This significantly reduces the risk of water quality deterioration and the possibility of reduced survival rates or even total extinction of snails due to water quality issues, greatly reducing the risk of aquaculture. The sand-free aquaculture method of this invention eliminates the problems of sand layer contamination and blackening, as well as excessive levels of harmful bacteria, reducing the probability of infectious diseases and providing a healthier growth environment for snails. This improves the survival rate of snails, thereby increasing aquaculture yield and improving aquaculture efficiency. Furthermore, it eliminates the need to treat contaminated sand layers, saving labor and material costs and further enhancing the economic benefits of aquaculture.

[0028] Preferably, the stocking density of the whelk is 1000-1500 individuals / m². 2 .

[0029] Preferably, the amount of the growth regulator added is 5-20 g / m². 3 It should be added every 7-15 days. The dosage of the growth regulator is 5-20 g / m². 3 Indicates each m 3 Add 5-20g of growth regulator to the water volume.

[0030] Preferably, the aquaculture pond can achieve water recycling.

[0031] A recirculating aquaculture system for conch is provided. Specifically, this invention provides a breeding pond that enables water recycling as a reference. The breeding pond is a racetrack-style pond with an arc-shaped perimeter. There is a partition wall in the middle of the pond. The perimeter and bottom of the pond are painted black, and a water treatment tank is provided at one end of the pond.

[0032] Compared with traditional square ponds, the racetrack-style aquaculture pond described in this invention is conducive to full water circulation and eliminates dead water in dead corners.

[0033] Preferably, the breeding pond is equipped with a light bulb that gradually brightens from a dim light when turned on and gradually dims when turned off. Painting the sides and bottom of the breeding pond black, along with the gradual on / off switching of the light bulb, helps reduce stress on the snails, providing them with a more comfortable environment. This, combined with growth regulators, yields even better results.

[0034] Preferably, the water treatment tank is divided into two compartments: a treatment compartment and a purification compartment. The treatment compartment includes an ultraviolet lamp disinfection device, a protein separator, and a filter module. The purification compartment includes an aeration device.

[0035] Preferably, the filter module consists of a fine sand layer, a coral stone layer, and an activated carbon layer from bottom to top.

[0036] Preferably, the aquaculture pond is darkened around its perimeter and top to create a light-free environment. A humidity detector is installed indoors, and ventilation fans are turned on when the humidity is ≥60% to control the indoor humidity below 60%.

[0037] Preferably, the inlet of the aquaculture pond is equipped with a water flow sensor, which detects the amount of new water entering and the amount of old water being discharged in a single-pond circulating system to achieve a balance in the total water volume. The water flow sensor helps to better utilize water and reduce energy consumption (electricity).

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] (1) This invention provides a growth regulator that, through the combined use of lentinan, taurine, lysine, ethyl m-aminobenzoate methanesulfonate, and Eucommia ulmoides leaf extract, can significantly improve the survival rate and weight of snails in the culture of Oriental Wind Snail.

[0040] (2) This invention provides a sand-free aquaculture method that eliminates the problems of sand layer contamination and blackening, as well as excessive levels of harmful bacteria, thereby reducing the probability of infectious diseases, providing a healthier growth environment for the snails, improving the survival rate of the snails, increasing aquaculture yield, and improving aquaculture efficiency. Furthermore, it eliminates the need to treat contaminated sand layers, saving manpower and material costs, and further enhancing the economic benefits of aquaculture. Sand-free aquaculture also facilitates the observation of the growth status and health condition of the snails, which is conducive to timely detection of problems and the implementation of corresponding measures. It simplifies the aquaculture process, making aquaculture management more convenient and efficient, and helps to improve aquaculture efficiency. Attached Figure Description

[0041] Figure 1 This is a schematic diagram of the sand-free culture method for Middle Eastern whelks according to the present invention.

[0042] Figure 2 This is a schematic diagram of the traditional sand-based farming method for Oriental whelks. Detailed Implementation

[0043] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0044] Example 1: Preparation of growth regulators

[0045] By weight: mix 1 part vitamin E, 1 part vitamin C, 6 parts potassium chloride, 6 parts citric acid, 1 part lentinan (source: Shanghai Aladdin Biochemical Technology Co., Ltd.), 4 parts taurine, 5 parts lysine, 2 parts ethyl m-aminobenzoate methanesulfonate, and 10 parts Eucommia ulmoides leaf extract (source: Shaanxi Pinhong Biotechnology Co., Ltd.) to obtain the growth regulator.

[0046] Example 2: Preparation of growth regulators

[0047] By weight: mix 1.5 parts vitamin E, 1.5 parts vitamin C, 10 parts potassium chloride, 6 parts citric acid, 2 parts lentinan (source: Shanghai Aladdin Biochemical Technology Co., Ltd.), 6 parts taurine, 8 parts lysine, 3 parts ethyl m-aminobenzoate methanesulfonate, and 13 parts Eucommia ulmoides leaf extract (source: Shaanxi Pinhong Biotechnology Co., Ltd.) to obtain the growth regulator.

[0048] Example 3: Preparation of growth regulators

[0049] By weight: mix 2 parts vitamin E, 2 parts vitamin C, 15 parts potassium chloride, 7 parts citric acid, 3 parts lentinan (source: Shanghai Aladdin Biochemical Technology Co., Ltd.), 8 parts taurine, 10 parts lysine, 4 parts ethyl m-aminobenzoate methanesulfonate, and 15 parts Eucommia ulmoides leaf extract (source: Shaanxi Pinhong Biotechnology Co., Ltd.) to obtain the growth regulator.

[0050] Example 4: Preparation of growth regulators

[0051] The difference between this embodiment and Embodiment 1 is that the growth regulator does not contain vitamin E and vitamin C, while the remaining ingredients and quantities are the same as in Embodiment 1.

[0052] Example 5: Preparation of growth regulators

[0053] The difference between this embodiment and Example 1 is that the growth regulator does not contain potassium chloride or citric acid, while the remaining raw materials and proportions are the same as in Example 1.

[0054] Example 6: Preparation of growth regulators

[0055] The difference between this embodiment and Embodiment 1 is that the growth regulator does not contain potassium chloride, citric acid, vitamin E, or vitamin C, while the remaining ingredients and quantities are the same as in Embodiment 1.

[0056] Example 7: A Sand-Free Culture Method for Oriental Wind Snails

[0057] Multiple 0.5m x 0.5m x 0.5m PVC square culture tanks were custom-made. Each tank was equipped with a water inlet pipe, a drain pipe, and an air inlet pipe. Each tank had a corresponding cover. A shade net or canvas was laid on top of the system to prevent excessive sunlight from affecting the growth of the spotted whelks. No sand layer was laid on the bottom of the tanks. The tanks were divided into three equal sections by a glass plate, with 100 spotted whelk larvae cultured in each section. The larvae population was 800 individuals per jin (approximately 500 individuals per catties). The snails were fed with mackerel every two days at a rate of 10% of their body weight. The growth regulator prepared in Example 1 was used at a dosage of 15 grams per cubic meter of water, applied every 10 days for a continuous culture period of 7 months. A detailed culture diagram is shown below. Figure 1 As shown.

[0058] Example 8: A Sand-Free Culture Method for Oriental Wind Snails

[0059] The difference between this embodiment and Example 7 is that the growth regulator prepared in Example 2 is used.

[0060] Example 9: A Sand-Free Cultivation Method for Oriental Wind Snails

[0061] The difference between this embodiment and Example 7 is that the growth regulator prepared in Example 3 is used.

[0062] Example 10: A Sand-Free Culture Method for Oriental Wind Snails

[0063] The difference between this embodiment and Example 7 is that the growth regulator prepared in Example 4 is used.

[0064] Example 11: A Sand-Free Culture Method for Oriental Wind Snails

[0065] The difference between this embodiment and Example 7 is that the growth regulator prepared in Example 5 is used.

[0066] Example 12: A Sand-Free Culture Method for Oriental Wind Snails

[0067] The difference between this embodiment and Example 7 is that the growth regulator prepared in Example 6 is used.

[0068] Example 13: A Sand-Based Cultivation Method for Oriental Wind Snails

[0069] Multiple 0.5m × 0.5m × 0.5m PVC square culture tanks were custom-made. Each tank was equipped with a water inlet pipe, a drain pipe, and an air inlet pipe. Each tank had a corresponding cover. A shade net or canvas was laid over the tank to prevent excessive sunlight from affecting the growth of the spotted whelks. First, a layer of wooden planks was laid at the bottom of the tank, covered with 80-mesh silk sieve, and then a layer of sand was laid on top. Before culture, the sand and tank bottom were disinfected with potassium permanganate and rinsed clean. The tanks were divided into three equal sections by glass partitions, with 100 spotted whelk larvae cultured in each section. The larvae population was 800 individuals per jin (approximately 500 individuals per 500 grams). The snails were fed with mackerel every two days, at a rate of 10% of their body weight. The growth regulator prepared in Example 1 was used at a dosage of 15 grams per cubic meter of water, applied every 10 days, for a continuous culture period of 7 months.

[0070] Comparative Example 1: A Sand-Based Cultivation Method for *Sinocyclocheilus edulis*

[0071] Multiple 0.5m x 0.5m x 0.5m PVC square culture tanks are custom-made. Each tank is equipped with a water inlet pipe, a drain pipe, and an air inlet pipe. Each tank has a corresponding cover. A shade net or canvas is laid on top of the system to prevent excessive sunlight from affecting the growth of the spotted whelks. First, a layer of wooden boards is laid at the bottom of the tank, covered with 80-mesh silk sieve, and then a layer of sand is laid on top. Before culture, the sand and tanks at the bottom are disinfected with potassium permanganate and rinsed clean. The tanks are divided into three equal sections by a glass plate, with 100 spotted whelk larvae cultured in each section. The larvae size is 800 larvae / catties. They are fed with mackerel every two days, with each feeding amount being 10% of the whelk's body weight. No growth regulators are used, and the culture is continued for 7 months. A detailed culture diagram is shown below. Figure 2 As shown.

[0072] Comparative Example 2

[0073] The difference between this comparative example and Example 12 is that no growth regulator was used.

[0074] Comparative Example 3

[0075] The difference between this comparative example and Example 12 is that the growth regulator does not contain lentinan.

[0076] Comparative Example 4

[0077] The difference between this comparative example and Example 12 is that the growth regulator does not contain taurine and lysine.

[0078] Comparative Example 5

[0079] The difference between this comparative example and Example 12 is that the growth regulator does not contain ethyl m-aminobenzoate methanesulfonate.

[0080] Comparative Example 6

[0081] The difference between this comparative example and Example 12 is that the growth regulator does not contain Eucommia ulmoides leaf extract.

[0082] Test Example 1

[0083] Each breeding tank must be meticulously inspected daily, and any dead snails must be removed promptly. Simultaneously, oxygen levels, water flow, and water temperature must be carefully checked and recorded in detail. After seven months of breeding, the number and weight of the surviving individuals should be counted.

[0084] The above statistical results are shown in Table 1 below.

[0085] Table 1. Survival rate and snail weight of farmed *Bellamya spp.* in different embodiments and comparative examples.

[0086] Number of survivors / animals Harvested snails / gram Number of survivors / animals Harvested snails / gram Example 7 290 3259.5 Example 8 295 3312.8 Example 9 275 3193 Example 10 197 2099.2 Example 11 202 2195 Example 12 195 2061 Example 13 261 2932.9 Comparative Example 1 187 1993.3 Comparative Example 2 90 906.5 Comparative Example 3 110 1128.5 Comparative Example 4 145 1478.5 Comparative Example 5 183 1908 Comparative Example 6 170 1780

[0087] As can be seen from Examples 7 to 13 above, adding the growth regulator of the present invention to the water used for the cultivation of African whelks can greatly improve the survival rate and weight of the whelks.

[0088] Compared with Example 7, Example 10 differs in that the growth regulator does not contain vitamin E and vitamin C; compared with Example 7, Example 11 differs in that the growth regulator does not contain potassium chloride and citric acid; compared with Example 7, Example 12 differs in that the growth regulator does not contain potassium chloride, citric acid, vitamin E, and vitamin C. The experimental results in Table 1 show that although the number of surviving snails and their weight in Examples 10-12 were slightly lower than in Example 7, they were still better than traditional sand-cultured snails without the growth regulator of this application (Comparative Example 1). This indicates that Eucommia ulmoides leaf extract, ethyl m-aminobenzoate methanesulfonate, taurine, lysine, and lentinan, as growth regulators, can significantly improve the number of surviving snails and their weight (compared to Comparative Example 2 without growth regulators). Furthermore, adding vitamin E and vitamin C (Example 10), potassium chloride and citric acid (Example 11), or vitamin E, vitamin C, potassium chloride, and citric acid (Example 7) can further improve the survival rate and weight of snails in sand-free culture.

[0089] As can be seen from Examples 12, 3, 4, 5 and 6, the combined use of Eucommia ulmoides leaf extract, ethyl m-aminobenzoate methanesulfonate, taurine, lysine and lentinan can synergistically promote the survival rate and weight of the snail. However, removing one or more of these ingredients has a limited effect on promoting the survival rate and weight of the snail, making it difficult to achieve the technical effect of the present invention.

[0090] Example 13 shows that the addition of growth regulators during sand culture significantly increased the number of snails and their weight compared to Comparative Example 1, which showed sand culture without growth regulators.

[0091] This invention provides a growth regulator that, through the combined use of Eucommia ulmoides leaf extract, ethyl m-aminobenzoate methanesulfonate, taurine, lysine, and lentinan, can meet the nutritional needs of the Oriental Wind Snail and improve its appetite, thereby increasing its survival rate and snail weight. Its effect is superior to that of traditional sand-based aquaculture.

[0092] The foregoing examples are merely illustrative, used to explain some features of the method described in this invention. The appended claims are intended to claim the broadest possible scope, and the embodiments presented herein are demonstrated by the applicant's actual experimental results. Therefore, the applicant intends that the appended claims are not limited by the selection of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within them, and variations within these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. The application of a growth regulator in sand-free culture of *Bellamya aegyptiaca*, characterized in that, The growth regulator is composed of the following raw materials in parts by weight: 1-3 parts lentinan, 4-8 parts taurine, 5-10 parts lysine, 2-4 parts ethyl m-aminobenzoate methanesulfonate, 10-15 parts Eucommia ulmoides leaf extract, 1-2 parts vitamin E, 1-2 parts vitamin C, 6-15 parts potassium chloride, and 3-7 parts citric acid.

2. The application according to claim 1, characterized in that, The growth regulator is prepared by mixing the raw materials according to the specified weight proportions.

3. A method for sand-free cultivation of *Sinocyclocheilus edulis*, characterized in that, The growth regulator described in claim 1 is added to the aquaculture pond, and no sand is laid in the aquaculture pond.

4. The method according to claim 3, characterized in that, The amount of the growth regulator added is 5-20 g / m². 3 Add it every 7-15 days.

5. The method according to claim 3, characterized in that, The stocking density of the Oriental whelk is 1000-1500 individuals / m². 2 .

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