Method for monitoring intracellular NADH (Nicotinamide Adenine Dinucleotide Hormone) in real time based on nanowire coupled photoelectrochemical sensor

Abstract

The invention provides a method for monitoring intracellular NADH (Nicotinamide Adenine Dinucleotide Hormone) in real time based on a nanowire coupled photoelectrochemical sensor, and belongs to the technical field of biosensors. According to the method, a nanowire MWCNT (at) FITC-PFBT Pdots-PDDA-Pt NWs capable of being semi-embedded into a cell spontaneously and losslessly is used, the nanowire and NADH in the cell are subjected to an oxidation reaction, and an electron transfer event is generated. A photosensitive electrode Flex ITO (indium tin oxide)-WO3 / BiVO4 covering the surface of a cell is utilized to gather an electric signal from a nanowire, and an oxidation reaction in the cell is accelerated, so that the current response is improved. By means of the method, real-time monitoring of the NADH in the cells can be achieved. The method has the advantages of low cost, simplicity in operation, high sensitivity and the like, and is widely applicable to detection of signal molecules in cells.