SgRNA and CRISPR / Cas9 gene editing system targeting portunus trituberculatus scarlet gene and application of SgRNA and CRISPR / Cas9 gene editing system

Abstract

The invention provides an SgRNA and CRISPR / Cas9 gene editing system targeting a portunus trituberculatus scarlet gene and application of the SgRNA and CRISPR / Cas9 gene editing system, and belongs to the technical field of gene editing. The SgRNA of the target portunus trituberculatus scarlet gene is characterized in that the sequence of the SgRNA of the target portunus trituberculatus scarlet gene is as shown in SEQ ID No. 1 to SEQ ID No. 6. According to the method, single-gene multi-target mixed injection is carried out in the portunus trituberculatus through the CRISPR / Cas9 technology, scarlet gene knockout is achieved, the mutant with eye development defects is obtained, the mutant is obvious in character and effect, and it is marked that the gene editing technology obtains a new breakthrough in the portunus trituberculatus. The technical scheme provided by the invention has an important reference value for the application of a gene editing technology in crustacean.

Images