Real-time fluorescent PCR (polymerase chain reaction) detection method for avian pox virus and primers for detection

Abstract

The invention discloses a real-time fluorescent PCR (polymerase chain reaction) detection method and detection primers for avian pox viruses, and the detection method has a good linear relationship; compared with conventional PCR, the fluorescent quantitative PCR detection sensitivity can be improved by 100 times, the detected plasmid standard substance with the lowest virus content of 9.75 TCID50 per milliliter and 3.53 copies / microliter can be achieved, the method is used for detecting common virus samples such as FPV, PPV, HVT and ILTV, except that the FPV and PPV samples are positive, the other samples are negative, and good specificity is achieved; the coefficient of variation of intra-group and inter-group repeatability tests is less than 1.57%; when the kit is used for detecting 186 clinical samples, the qPCR positive detection rate is 65.1% (121 / 186), the conventional PCR positive detection rate is 55.9% (104 / 186), and the total coincidence rate of the qPCR positive detection rate and the conventional PCR positive detection rate is 90.9%.

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