A method for clarifying turbid liquid and its application

Abstract

This invention discloses a method for clarifying turbid liquids and its application. The clarification method provided by this invention includes the following steps: taking the isolated turbid liquid, adding a reagent, and stirring to clarify the turbid liquid; the reagent is an aqueous solution containing polyvinylpyrrolidone; the turbid liquid contains blood, body fluids, and physiological saline. This invention discovers that by adding polyvinylpyrrolidone to a turbid liquid containing blood and body fluids, the turbid liquid can be rapidly made clear and transparent. Using this method can ensure a clear endoscopic field of view during surgery, reduce the difficulty of surgery, ensure surgical efficacy, and increase the surgical safety factor.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a method for clarifying a turbid liquid containing blood or body fluids, and the application of this method. Background Technology

[0002] An endoscope is an optical instrument consisting of a cold light source lens, fiber optic cable, image transmission system, and screen display system. With the assistance of endoscopes and specialized surgical instruments, surgeries that traditionally required large incisions can be completed with only tiny wounds. This not only reduces surgical trauma but also increases surgical precision and safety, thus enabling patients to recover more quickly.

[0003] During endoscopic procedures, bleeding from the wound is common. Clinically, normal saline is typically used for irrigation to ensure a clear endoscopic view. However, in cases of significant bleeding or poor fluid return, the mixture of normal saline and blood / other bodily fluids can severely impair the endoscopic view, making the procedure difficult and impacting both surgical efficacy and patient safety. Therefore, ensuring a clear endoscopic view during surgery is a crucial requirement. Summary of the Invention

[0004] The purpose of this invention is to provide a method for clarifying turbid fluids containing blood and body fluids. This method can ensure a clear endoscopic field of view during surgery, reduce the difficulty of surgery, ensure the efficacy of surgery, and increase the safety factor of surgery.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: The present invention provides a method for clarifying a turbid liquid, comprising the following steps: taking an isolated turbid liquid, adding a reagent, and stirring to clarify the turbid liquid; The reagent is an aqueous solution containing polyvinylpyrrolidone; The turbid liquid contains blood, body fluids, and saline solution.

[0006] In one embodiment, the turbid fluid can be obtained by draining it from the surgical field through a drainage device, and the clarification method does not include the step of obtaining the turbid fluid from the body or the step of subsequently using the clarified fluid.

[0007] In the above method, the amount of reagent added is 24-25 ml of reagent per 1 ml of turbid liquid.

[0008] In the above method, the stirring conditions are: temperature 18-35℃ and time 8-15s.

[0009] The present invention also provides a reagent for endoscopic surgery, comprising physiological saline and polyvinylpyrrolidone; wherein the mass concentration of the polyvinylpyrrolidone is 1%.

[0010] The reagent is one or more of the following: iodine dilution, polyvinylpyrrolidone (PVP) solution, cross-linked polyvinylpyrrolidone (PVP-P) solution, and povidone-iodine dilution.

[0011] In the reagent, the iodine diluent is an aqueous solution formed by diluting medical iodine stock solution with physiological saline by 50-200 times.

[0012] In one embodiment, the iodine diluent is a solution formed by diluting the iodine stock solution with physiological saline by 50, 100, or 200 times.

[0013] The iodine solution can be medical iodine solution, with a concentration of 0.5%, and its main components are polyvinylpyrrolidone and elemental iodine.

[0014] The reagents are used to describe procedures including ureteroscopic lithotripsy, transurethral resection of bladder tumor, percutaneous nephrolithotomy, cystoscopy, hysteroscopy, or all endoscopic procedures performed in aqueous media.

[0015] The present invention further provides the use of the aforementioned endoscopic surgical reagent in the preparation of endoscopic clarifying solutions for endoscopic surgery.

[0016] The endoscopic surgical reagent is mixed with the turbid liquid generated during the endoscopic operation to clarify the turbid liquid, forming an endoscopic clarifying solution.

[0017] Compared with the prior art, the beneficial effects achieved by the present invention are: Polyvinylpyrrolidone (PVP), as a synthetic water-soluble polymer, possesses the general properties of water-soluble polymers and is widely used in the pharmaceutical, food, and cosmetic fields. In the pharmaceutical field, it is widely used as an excipient in drug formulations; additionally, PVP can also be used as a coloring agent and X-ray contrast agent; it can be used in various dosage forms such as tablets, granules, and liquids, and has effects such as detoxification, hemostasis, increasing solubility concentration, preventing peritoneal adhesions, and promoting erythrocyte sedimentation rate.

[0018] However, this invention unexpectedly discovered that adding an aqueous solution containing polyvinylpyrrolidone, such as diluted povidone-iodine, to a turbid solution containing blood, body fluids, or other components can rapidly clarify the turbidity. Applying this discovery to endoscopic surgery ensures a clear endoscopic view, solves clinical problems, improves surgical safety, and has significant potential for widespread application. Attached Figure Description

[0019] Figure 1 The appearance of iodine solution diluted at different dilution ratios.

[0020] Figure 2The appearance of blood samples after adding iodine solution of different dilution ratios.

[0021] Figure 3 The curve shows the relationship between the dilution concentration of iodine solution and the relative transmittance of the blood sample.

[0022] Figure 4 The graph shows the transmittance of blood samples after adding different dilution factors of povidone-iodine solution. In the figure, the horizontal axis represents the blood samples after adding dilution factors of povidone-iodine solution, and the vertical axis represents the relative transmittance of each mixed solution to the transmittance of physiological saline, with physiological saline transmittance defined as 100%.

[0023] Figure 5 The appearance of a blood sample after adding 1% polyvinylpyrrolidone and an iodine-containing aqueous solution.

[0024] Figure 6 The transmittance of the blood sample after adding 1% polyvinylpyrrolidone and an iodine-containing aqueous solution. Detailed Implementation

[0025] The present invention will be further described below with reference to specific embodiments, but the present invention is not limited to the following embodiments.

[0026] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0027] Unless otherwise specified, all reagents, materials, instruments, etc. used in the following examples are commercially available.

[0028] Example 1: Iodine dilution solution used for blood sample clarification The specific steps are as follows: (1) Dilute the medical iodine solution to 10, 50, 100, and 200 times its original volume using physiological saline to obtain iodine solutions of different concentrations; such as Figure 1 The solutions shown are 1:10 iodine solution, 1:50 iodine solution, 1:100 iodine solution, and 1:200 iodine solution.

[0029] The medical povidone-iodine solution (concentration 0.5%) used in this embodiment is mainly composed of polyvinylpyrrolidone and elemental iodine.

[0030] (2) Five turbid blood samples were collected from outside the body, each with a volume of 200 μL. Physiological saline (0.9% NaCl), 1:10 iodine solution, 1:50 iodine solution, 1:100 iodine solution, and 1:200 iodine solution were added in sequence, with a volume of 4.8 ml for each sample. The resulting mixtures were labeled as: (A) blood sample + 0.9% NaCl, (B) blood sample + 1:10 iodine dilution, (C) blood sample + 1:50 iodine dilution, (D) blood sample + 1:100 iodine dilution, and (E) blood sample + 1:200 iodine dilution. The mixtures were stirred at room temperature for 15 seconds.

[0031] The transmittance test results for each mixture are as follows: (1) Visual observation: such as Figure 2 As shown, by comparing the transparency of the bottom of the cuvette and the black background on the table, it can be seen that the blood sample with added saline is still opaque, while the blood sample with added iodine diluent has varying degrees of increased transparency, and the bottom of the cuvette and the black background on the table become visible. Among them, the blood sample with added 1:100 iodine diluent has the most significant increase in transparency.

[0032] (2) Spectrophotometer measurement: The transmittance of 0.9% sodium chloride (NaCl) solution was defined as 100%. The relative transmittance of the blood sample after adding iodine dilution was measured and calculated. The relationship curve between the iodine dilution concentration and the relative transmittance of the blood sample is shown in the figure. Figure 3 As shown, the calculation formula is as follows: y = -1028.6x 2 + 100.87x + 0.3021, where: y is the relative transmittance, and x is the concentration of iodine tincture. The calculation results are as follows Figure 4 As shown, the transmittance of blood samples diluted with 0.9% NaCl, 1:10 iodine solution, 1:50 iodine solution, 1:100 iodine solution, and 1:200 iodine solution were 0, 0.115, 1.331, 2.411, and 0.445, respectively. It can be seen that iodine solutions diluted 50 and 100 times can effectively improve the transmittance of turbid blood samples, with the 100-fold dilution showing the best clarification effect.

[0033] Example 2: Use of polyvinylpyrrolidone solution for blood sample clarification The specific steps are as follows: Three turbid blood samples were collected from outside the body, each 1 ml. 24 ml each of 1% polyvinylpyrrolidone solution, 0.4 mol / L iodine-containing aqueous solution, and 0.9% sodium chloride (NaCl) solution were added sequentially, and the mixture was stirred at room temperature for 15 seconds.

[0034] The results are as follows Figure 5 , Figure 6 As shown, the transmittance of blood samples with the addition of 1% polyvinylpyrrolidone solution increased, while the transmittance of blood samples with the addition of iodine solution did not change substantially. Further measurements using a spectrophotometer, with the transmittance of 0.9% sodium chloride (NaCl) solution defined as 100%, showed that the transmittance of blood samples with 0.9% NaCl, 0.4 mol / L iodine, and 1% polyvinylpyrrolidone solution were 0.003, 0.005, and 1.170, respectively.

[0035] Meanwhile, the present invention also attempted to use other aqueous solutions containing polyvinylpyrrolidone for testing, and the clarification effect was basically the same as that of 1% polyvinylpyrrolidone solution on blood samples.

[0036] In summary, the above experiments demonstrate that polyvinylpyrrolidone can rapidly clarify and brighten turbid fluids containing blood, body fluids, and other components. This discovery can be used to ensure a clear endoscopic field of view during endoscopic surgery, thereby reducing the difficulty of the surgery, ensuring its efficacy, and increasing its safety.

[0037] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. The use of a surgical reagent in the preparation of an endoscopic clarifying solution for endoscopic surgery; The surgical reagent is an aqueous solution made by diluting medical iodine solution containing polyvinylpyrrolidone with physiological saline to 50-100 times its volume. The surgical reagent is mixed with the turbid liquid generated during the endoscopic procedure to clarify the turbid liquid, forming an endoscopic clarifying solution; The turbid liquid contains blood, body fluids, and saline solution.

2. The use according to claim 1, characterized in that, The procedures include ureteroscopic lithotripsy, transurethral resection of bladder tumor, percutaneous nephrolithotomy, cystoscopy, hysteroscopy, or all endoscopic procedures performed in aqueous media.

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