Efficient synthesis method and application of polylactic acid and / or polyhydroxybutyrate lactate

By adopting secondary fermentation and organic acid feeding methods in microbial synthesis, the problems of low yield of polylactic acid and polyhydroxybutyrate lactate and difficulty in regulating lactic acid components are solved, efficient and low-cost industrial production is achieved, and the material performance needs in different application scenarios are met.

CN119932124APending Publication Date: 2025-05-06HUAIYIN INSTITUTE OF TECHNOLOGY
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Patent Information

Application Number
CN202510067540.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-16
Publication Date
2025-05-06

AI Technical Summary

Technical Problem

The prior art faces the problems of low yield, high cost and difficulty in regulating lactic acid components in the microbial synthesis of polylactic acid and polyhydroxybutyric acid lactate, which is difficult to meet the needs of large-scale industrial production.

Method used

The synthesis method of polylactic acid and/or polyhydroxybutyrate lactate based on secondary fermentation and organic acid feeding is adopted to construct recombinant strains through gene knockout technology, polylactic acid synthesis pathway plasmid is introduced, and sodium lactate and/or sodium acetate are added for feeding during the fermentation process, and the fermentation process is optimized to increase yield and regulate the proportion of lactic acid components.

Benefits of technology

The yield of polylactic acid and polyhydroxybutyrate lactic acid esters has been significantly improved, with an increase of 324% and 423% compared with the primary fermentation of the original bacteria, and the precise regulation of the proportion of lactic acid components has been achieved to meet the needs of different application scenarios.

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Abstract

The invention discloses an efficient synthesis method and application of polylactic acid and polyhydroxybutyrate lactate. Specific genes in escherichia coli are knocked out through a gene knockout technology, polylactic acid and polyhydroxybutyrate lactate are synthesized into pathway plasmids, and the pathway plasmids are transferred into host bacteria to construct recombinant engineering bacteria. Through a secondary fermentation technology, sodium lactate, sodium acetate and other organic acid salts are added in the fermentation process for feeding, the metabolic pathway of thalli is optimized, the yield of polylactic acid and polyhydroxybutyrate lactate is remarkably increased, and precise regulation and control of lactic acid components are achieved. According to the method, the yield of polylactic acid can be increased by 324% compared with that of primary fermentation of original bacteria, the yield of polyhydroxybutyrate lactate is increased by 423%, and meanwhile, the proportion of a lactic acid component in the polyhydroxybutyrate lactate is precisely regulated and controlled within 17.3 mol%-70.0 mol%. The invention provides an efficient and feasible technical scheme for industrial production of polylactic acid products.
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Citation Information

Patent Citations

  • Engineered escherichia coli for synthesizing pure polylactic acid from lactic acid as well as preparation method and application of engineered escherichia coli

    CN114196609A

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