Health-care product capable of relieving fatigue and enhancing immunity and preparation method thereof

Abstract

This invention discloses a health product that can relieve fatigue and enhance immunity, and its preparation method. The health product comprises the following raw materials in parts by weight: 15-25 parts of compound enzyme powder, 5-10 parts of Cordyceps militaris polypeptide, 1-3 parts of stevioside, and 15-20 parts of maltodextrin. The compound enzyme powder is prepared by fermentation with *Lactobacillus rhamnosus* NJ551, using hibiscus extract and *Schisandra chinensis* extract as raw materials. The health product of this invention contains a variety of natural nutrients, formulated and refined through scientific proportioning. These components have significant antioxidant, anti-fatigue, and immune-enhancing effects, effectively relieving physical fatigue and improving the body's immunity.

Description

Technical Field

[0001] This invention belongs to the field of health products, specifically relating to a health product that can relieve fatigue and enhance immunity, and its preparation method. Background Technology

[0002] With the fast pace of modern life and increasing work pressure, people are increasingly prone to fatigue and weakened immunity. Fatigue and low immunity not only affect people's quality of life but may also lead to various diseases. Therefore, developing a health product that can relieve fatigue and enhance immunity is of great significance.

[0003] Currently, there are several research reports on health supplements that can relieve fatigue and enhance immunity. For example, some studies have shown that ingredients such as *Paecilomyces hepiali* powder, American ginseng, *Epimedium*, and *Gynostemma pentaphyllum* can significantly improve immune function and relieve physical fatigue through synergistic effects. Furthermore, the combination of coenzyme Q10 and grape seed extract has also been shown to have significant anti-fatigue and immune-enhancing effects; this combination achieves its efficacy through antioxidant activity and regulation of the immune system. In addition, some traditional Chinese medicine ingredients such as astragalus, rhodiola, and ganoderma are also widely used in health supplements for anti-fatigue and immune enhancement. These ingredients typically have functions such as replenishing qi and yin, and nourishing the liver and kidneys, achieving anti-fatigue and immune-enhancing effects by regulating endocrine function and improving microcirculation. However, these products vary greatly in terms of efficacy, taste, ease of absorption, and safety, making it difficult for consumers to choose. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a health product with scientific ingredients, significant effects, good taste, easy absorption by the human body, and high safety, so as to meet people's needs for relieving fatigue and enhancing immunity.

[0005] The technical solution of this invention is:

[0006] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 15-25 parts of compound enzyme powder, 5-10 parts of Cordyceps militaris polypeptide, 1-3 parts of stevioside and 15-20 parts of maltodextrin.

[0007] Preferably, the health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 20 parts of compound enzyme powder, 8 parts of cordyceps militaris polypeptide, 2 parts of stevioside and 15 parts of maltodextrin.

[0008] Preferably, the preparation steps of the compound enzyme powder are as follows:

[0009] (1) After soaking dried, mold-free roselle in water, extract it by hot water, filter it, concentrate and dry the filtrate to obtain roselle extract;

[0010] (2) Wash the Schisandra chinensis clean, dry it and crush it. Add an appropriate amount of water and reflux to extract it. Filter it and concentrate and dry the filtrate to obtain Schisandra chinensis extract.

[0011] (3) Dissolve roselle extract, schisandra extract and glucose in water, stir and mix well, and pasteurize to obtain fermentation medium;

[0012] (4) Inoculate Lactobacillus rhamnosus into the fermentation medium for fermentation, centrifuge the fermentation product, collect the supernatant and dry it to obtain compound enzyme powder.

[0013] Furthermore, the mass ratio of the roselle extract, schisandra extract, glucose, and water is 5:5:(3~4):100.

[0014] Furthermore, the viable count of the Lactobacillus rhamnosus is 10. 9 ~10 10 The inoculum size was CFU / mL, and the inoculum size of Lactobacillus rhamnosus was 3% by volume.

[0015] Furthermore, the pasteurization process involves heating at 65°C for 20 minutes.

[0016] Preferably, the preparation steps of the Cordyceps militaris polypeptide are as follows:

[0017] (1) Extract the selenium-enriched Cordyceps militaris powder with acetic acid solution, centrifuge, collect the supernatant, precipitate with saturated ammonium sulfate, centrifuge, collect the precipitate, redissolve the precipitate with pure water, and obtain selenium protein powder by dialysis and drying.

[0018] (2) Dissolve the selenoprotein dry powder in water, adjust the pH to 1.5~3, add pepsin for enzymatic hydrolysis, then adjust the pH to 7.8~8.5, add trypsin for further enzymatic hydrolysis, after inactivation of enzyme, filter, separate the filtrate with an ultrafiltration membrane with a molecular weight cutoff of 5 kDa~10 kDa, collect the components with molecular weights between 10 kDa and 5 kDa, dry, and obtain Cordyceps militaris polypeptide.

[0019] Furthermore, the acetic acid solution has a concentration of 5% by volume, and contains 0.1% β-mercaptoethanol by volume.

[0020] Preferably, the health product is a capsule or tablet.

[0021] The present invention also provides a method for preparing the above-mentioned health products that can relieve fatigue and enhance immunity, comprising the following steps: mixing the formulated amounts of compound enzyme powder, cordyceps militaris polypeptide, stevioside and maltodextrin evenly, filling them into capsule shells to obtain a capsule-formed health product, or adding an appropriate amount of tablet excipients and compressing them into tablets to obtain a tablet-formed health product.

[0022] Preferably, the tablet excipients include fillers, disintegrants, flow aids, and binders.

[0023] Preferably, the filler is microcrystalline cellulose, the disintegrant is croscarmellose sodium and / or polyvinylpyrrolidone, the flow aid is micronized silica gel, and the binder is a 5% PVP 60% ethanol solution.

[0024] Roselle, with its vibrant red color and abundant nutrients, is known as the 'ruby of the plant kingdom.' It is rich in vitamin C, flavonoids, anthocyanins, and other antioxidants. These components can eliminate free radicals in the body, reduce oxidative stress, thereby protecting cells from damage and improving the body's endurance and anti-fatigue ability. Flavonoids and anthocyanins, among other bioactive substances, have immunomodulatory effects, promoting the generation and function of immune cells, helping the body better cope with the invasion of external pathogens, improving the body's resistance to infection, and thus enhancing overall immunity.

[0025] Roselle enzyme is a common health food. Studies have shown that the components in roselle enzyme (such as alcohol, organic acids, fructose, saponin compounds, etc.) may irritate the gastrointestinal tract and cause symptoms such as diarrhea. In addition, roselle itself is cold in nature, and people with a weak constitution are prone to abdominal pain, diarrhea and other discomfort after drinking it. Therefore, although roselle has certain effects on anti-fatigue and enhancing immunity, the health food made in the form of enzyme is suitable for a limited number of people.

[0026] Schisandra chinensis is rich in active ingredients such as quercetin, which can enhance energy metabolism, improve mitochondrial function, and increase muscle oxygen supply, thereby improving athletic performance and reducing fatigue. The polysaccharides in Schisandra chinensis can enhance the phagocytic activity of macrophages, promote lymphocyte transformation, and increase the number and activity of white blood cells, thus enhancing the body's ability to defend against disease. Its active ingredients (such as quercetin) can activate the PI3K-Akt and MAPK signaling pathways, which play important roles in energy metabolism, cell survival, and immune regulation. Quercetin further enhances its anti-fatigue and immune-regulating effects by increasing the expression levels of related proteins (such as p-PI3K and p-AKT).

[0027] In addition, Schisandra chinensis has an astringent effect on diarrhea. Its mechanism of action includes the astringent effect of its sour taste, its warming and tonifying effect on the spleen and kidneys, its inhibition of intestinal smooth muscle contraction, its adsorption of bacteria and toxins, and its combined effects of invigorating qi and promoting body fluid production. When combined with hibiscus to create a compound enzyme, it can inhibit the gastrointestinal discomfort caused by hibiscus enzymes. Furthermore, the warm nature of Schisandra chinensis can neutralize the cold nature of hibiscus, reducing the irritation of the enzymes on the gastrointestinal tract.

[0028] Therefore, the compound enzyme powder made from roselle and schisandra chinensis of this invention not only has excellent anti-fatigue and immune-enhancing effects, but also significantly reduces gastrointestinal adverse reactions and improves the safety of enzyme powder use.

[0029] Furthermore, conventional enzyme preparation processes typically involve washing fresh roselle or schisandra berries, draining them, mixing them with glucose and water, and then sealing them for natural fermentation. Naturally fermented enzymes not only have a lower content of active ingredients, but the fermentation process may also produce substances harmful to the human body, such as higher alcohols. This invention optimizes the preparation process of the compound enzyme. First, it uses water extraction to extract the active ingredients from roselle or schisandra berries separately, obtaining roselle or schisandra berry extracts. Then, using these extracts as raw materials, it adds *Lactobacillus rhamnosus* as a strain for fermentation to prepare the compound enzyme. Using this process, not only can the active ingredients of roselle and schisandra berries be fully dissolved, but it also helps to convert large molecules in the extracts into smaller molecules and generate new active ingredients, thereby significantly improving their absorption rate and bioavailability in the human body.

[0030] The Lactobacillus rhamnosus used in this invention is preferably Lactobacillus rhamnosus NJ551 (deposited at the China Center for Type Culture Collection, accession number CCTCC NO: M2016157). This strain not only has good gastrointestinal stability but also possesses certain immunomodulatory activity. Using this strain as the fermentation strain not only ensures good safety but also helps enhance the immune activity of the compound enzyme.

[0031] The Cordyceps militaris polypeptide of this invention is prepared from selenium-enriched Cordyceps militaris powder through protein extraction, enzymatic hydrolysis, and separation. This Cordyceps militaris polypeptide can increase ATP (adenosine triphosphate) production, enhance antioxidant capacity, and reduce the production of lactic acid, free radicals, and lipid peroxides, thereby achieving an anti-fatigue effect. It can also stimulate macrophage activation, promote T lymphocyte proliferation, and enhance humoral and cellular immunity. This invention uses ultrafiltration membranes with different molecular weight cutoffs to separate the enzymatic hydrolysis products, obtaining Cordyceps militaris polypeptides with molecular weights between 5 kDa and 10 kDa, less than 5 kDa, and greater than 10 kDa. It was found that although polypeptides with molecular weights less than 5 kDa are more easily absorbed by the human body, the Cordyceps militaris polypeptides with molecular weights between 5 kDa and 10 kDa have the best compatibility with the compound enzyme powder of this invention, showing better effects than the other two molecular weight polypeptides.

[0032] Furthermore, adding appropriate amounts of stevioside and maltodextrin to health supplements can help improve the taste and texture of the product and increase its solubility.

[0033] The present invention has the following beneficial effects:

[0034] The health product of this invention contains a variety of natural nutrients, which are scientifically formulated and finely processed. These ingredients have significant antioxidant, anti-fatigue, and immune-boosting effects, effectively relieving physical fatigue and improving the body's immunity.

[0035] The health product of this invention not only has significant health benefits, but also a good taste and safety. This product contains no chemical ingredients or additives, has no side effects on the human body, and is highly safe.

[0036] In conclusion, the health products of this invention have broad market prospects and application value, and can provide effective protection for people's health. Attached Figure Description

[0037] Figure 1 This is the result of the anti-fatigue test of the health food product of this invention. Compared with the blank group, * p < 0.05 ** p < 0.01.

[0038] Figure 2 This is the result of testing the immune-enhancing effects of the health food product of this invention. A represents the effect on mouse immunoglobulin IgG levels; B represents the effect on mouse immunoglobulin IgM levels; and C represents the effect on mouse NK cell activity. Compared with the control group, * p < 0.05 ** p < 0.01, *** p < 0.001. Detailed Implementation

[0039] The following examples are further illustrations of the present invention, but not limitations thereof. Unless otherwise specified, the components in the formulations of the following examples are all commercially available products.

[0040] Example 1

[0041] 1. Preparation of compound enzyme powder:

[0042] (1) After cleaning the dried, mold-free roselle, soak it in water for 1 hour, heat it to 80°C for 3 hours, filter it, concentrate and dry the filtrate to obtain roselle extract.

[0043] (2) Clean the Schisandra chinensis, dry it and crush it. Add an appropriate amount of water, heat it at 100℃ and reflux for 2 hours. Filter it, concentrate and dry the filtrate to obtain Schisandra chinensis extract.

[0044] (3) Take 50 g of roselle extract, 50 g of schisandra extract and 35 g of glucose and dissolve them in 1000 mL of water. Stir and mix well, and then pasteurize at 65℃ for 20 min to obtain the fermentation medium.

[0045] (4) Lactobacillus rhamnosus NJ551 (live count of 10) 9 ~10 10 The enzyme powder (CFU / mL) was inoculated into the fermentation medium at a volume fraction of 3% and fermented at 37°C for 72 h. The fermentation product was centrifuged, the supernatant was collected and freeze-dried to obtain the compound enzyme powder.

[0046] 2. Preparation of hibiscus enzyme powder:

[0047] (1) After cleaning the dried, mold-free roselle, soak it in water for 1 hour, heat it to 80°C for 3 hours, filter it, concentrate and dry the filtrate to obtain roselle extract.

[0048] (2) Take 50 g of roselle extract and 35 g of glucose and dissolve them in 1000 mL of water. Stir and mix well, and then pasteurize at 65℃ for 20 min to obtain the fermentation medium.

[0049] (3) Lactobacillus rhamnosus NJ551 (live count of 10) 9 ~10 10 Inoculate the fermentation medium with 3% (CFU / mL) of the product into the fermentation medium and ferment at 37°C for 72 h. Centrifuge the fermentation product, collect the supernatant and freeze-dry it to obtain roselle enzyme powder.

[0050] 3. Preparation of Schisandra chinensis enzyme powder:

[0051] (1) Clean the Schisandra chinensis, dry it and crush it. Add an appropriate amount of water, heat it at 100℃ and reflux for 2 hours. Filter it, concentrate and dry the filtrate to obtain Schisandra chinensis extract.

[0052] (2) Take 50 g of Schisandra chinensis extract and 35 g of glucose and dissolve them in 1000 mL of water. Stir and mix well, and then pasteurize at 65℃ for 20 min to obtain the fermentation medium.

[0053] (3) Lactobacillus rhamnosus NJ551 (live count of 10) 9 ~10 10 Schisandra chinensis enzyme powder was obtained by inoculating the fermentation medium with 3% (CFU / mL) at 37°C for 72 h, centrifuging the fermentation product, collecting the supernatant and freeze-drying it.

[0054] Example 2

[0055] Preparation of Cordyceps militaris polypeptides:

[0056] (1) After drying, the selenium-enriched Cordyceps militaris was pulverized and passed through an 80-mesh sieve to obtain selenium-enriched Cordyceps militaris dry powder. The dry powder was added to a 5% acetic acid solution (containing 0.1% β-mercaptoethanol) at a material-to-liquid ratio of 1:20 g / mL, stirred at 4℃ for 4 h, centrifuged, and the supernatant was collected. Ammonium sulfate was added to the supernatant to 75% saturation, stirred for 0.5 h, allowed to stand at 4℃ for 12 h, centrifuged, and the precipitate was collected. The precipitate was redissolved with purified water, dialyzed, and freeze-dried to obtain selenium protein dry powder.

[0057] (2) Dissolve selenoprotein powder in water to prepare a 10 mg / mL selenoprotein solution, adjust the pH to 2, add 3% pepsin by weight of selenoprotein powder, and enzymatically hydrolyze at 37℃ for 1 h. Then adjust the pH to 8, add 3% trypsin by weight of selenoprotein powder, and continue enzymatic hydrolysis at 37℃ for 1 h. After inactivation of enzyme, filter, and separate the filtrate with ultrafiltration membranes with molecular weight cutoff of 10 kDa and 5 kDa. Collect the components with molecular weight less than 5 kDa, the components with molecular weight between 5 kDa and 10 kDa, and the components with molecular weight greater than 10 kDa. Freeze-dry to obtain Cordyceps militaris polypeptides with different molecular weights.

[0058] Cordyceps militaris peptides with a molecular weight less than 5 kDa are designated as CMP1, those with a molecular weight between 5 kDa and 10 kDa are designated as CMP2, and those with a molecular weight greater than 10 kDa are designated as CMP3.

[0059] Example 3

[0060] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 15 parts of compound enzyme powder, 5 parts of Cordyceps militaris polypeptide CMP2, 1 part of stevioside and 15 parts of maltodextrin.

[0061] Preparation steps: Mix the formulated amounts of compound enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside, and maltodextrin evenly, and fill them into capsule shells to obtain a capsule-form health product.

[0062] Example 4

[0063] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 25 parts of compound enzyme powder, 10 parts of Cordyceps militaris polypeptide CMP2, 3 parts of stevioside and 20 parts of maltodextrin.

[0064] Preparation steps: Mix the formulated amounts of compound enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside, and maltodextrin evenly, and fill them into capsule shells to obtain a capsule-form health product.

[0065] Example 5

[0066] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 20 parts of compound enzyme powder, 8 parts of Cordyceps militaris polypeptide CMP2, 2 parts of stevioside and 15 parts of maltodextrin.

[0067] Preparation steps: Mix the formulated amounts of compound enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside, and maltodextrin evenly, and fill them into capsule shells to obtain a capsule-form health product.

[0068] Example 6

[0069] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 20 parts of compound enzyme powder, 8 parts of Cordyceps militaris polypeptide CMP1, 2 parts of stevioside and 15 parts of maltodextrin.

[0070] Preparation steps: Mix the formulated amounts of compound enzyme powder, Cordyceps militaris polypeptide CMP1, stevioside, and maltodextrin evenly, and fill the mixture into capsule shells to obtain a capsule-form health product.

[0071] Example 7

[0072] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 20 parts of compound enzyme powder, 8 parts of Cordyceps militaris polypeptide CMP3, 2 parts of stevioside and 15 parts of maltodextrin.

[0073] Preparation steps: Mix the formulated amounts of compound enzyme powder, Cordyceps militaris polypeptide CMP3, stevioside, and maltodextrin evenly, and fill the mixture into capsule shells to obtain a capsule-form health product.

[0074] Comparative Example 1

[0075] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 20 parts roselle enzyme powder, 8 parts cordyceps peptide CMP2, 2 parts stevioside and 15 parts maltodextrin.

[0076] Preparation steps: Mix the prescribed amounts of roselle enzyme powder, cordyceps militaris polypeptide CMP2, stevioside, and maltodextrin evenly, and fill the mixture into capsule shells to obtain a capsule-form health product.

[0077] Comparative Example 2

[0078] A health product that can relieve fatigue and enhance immunity includes the following raw materials in parts by weight: 20 parts of Schisandra chinensis enzyme powder, 8 parts of Cordyceps militaris polypeptide CMP2, 2 parts of stevioside and 15 parts of maltodextrin.

[0079] Preparation steps: Mix the prescribed amounts of Schisandra chinensis enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside, and maltodextrin evenly, and fill them into capsule shells to obtain a capsule-form health product.

[0080] Comparative Example 3

[0081] The health supplement in Comparative Example 3 differs from that in Example 5 in that the formula does not contain compound enzyme powder, while the other components remain unchanged.

[0082] Comparative Example 4

[0083] The health supplement in Comparative Example 4 differs from that in Example 5 in that the formula does not contain Cordyceps militaris peptide CMP2, while the other components remain unchanged.

[0084] Application Example 1: Fatigue Resistance Testing

[0085] Kunming mice weighing 18-22 g were randomly divided into three groups: Example 3-7 (T3-T7), Comparative Examples 1-4 (D1-D4), and a blank control group (CK), with 10 mice in each group. Mice in Example 3-7 and Comparative Examples 1-4 were administered the contents of the capsule-type health products prepared in Example 3-7 and Comparative Examples 1-4 by gavage at a dose of 1 g / kg body weight, once daily for 30 consecutive days. Mice in the blank control group were administered an equal volume of physiological saline by gavage. Thirty minutes after the last gavage, mice in each group were placed in a swimming tank with a lead wire bearing 5% of their body weight loaded at the base of their tails until they could no longer swim (e.g., died of exhaustion or fainted). The weighted swimming time for each group was measured, and the average value was taken (x̄ ± 0.5%). - (±s), results are shown in Figure 1 .

[0086] Depend on Figure 1 It can be seen that the health products prepared in Examples 3-5 can all increase the swimming time of mice under weight-bearing exhaustion, with the health product prepared in Example 5 showing the best effect. Examples 5-7 show that, compared to Cordyceps militaris polypeptide (CMP1) with a molecular weight less than 5 kDa and Cordyceps militaris polypeptide (CMP3) with a molecular weight greater than 10 kDa, Cordyceps militaris polypeptide (CMP2) with a molecular weight between 5 kDa and 10 kDa has the best compatibility with the compound enzyme powder of the present invention, and its effect is superior to the other two molecular weight polypeptides. Comparative Examples 1-2 show that, compared to roselle enzyme powder and Schisandra chinensis enzyme powder, the compound enzyme powder of the present invention is more effective in increasing the swimming time of mice under weight-bearing exhaustion. Comparative Examples 3-4 show that the compound enzyme powder of the present invention and Cordyceps militaris polypeptide CMP2 have a synergistic effect in increasing the swimming time of mice under weight-bearing exhaustion.

[0087] Application Example 2: Immunity Enhancement Test

[0088] Kunming mice weighing 18-22 g were randomly divided into three groups: Example 3-7 (T3-T7), Comparative Examples 1-4 (D1-D4), and a blank control group (CK), with 10 mice in each group. Mice in Example 3-7 and Comparative Examples 1-4 were administered the contents of the capsule-type health products prepared in Example 3-7 and Comparative Examples 1-4 by gavage at a dose of 1 g / kg body weight, once daily for 30 consecutive days. Mice in the blank control group received an equal volume of physiological saline via gavage. On the second day after gavage, blood was collected from the mice by enucleation. After standing for 2 hours, the blood was centrifuged at 0.4×g for 20 min, and the supernatant was centrifuged at 0.4×g for 5 min. The supernatant was then analyzed using an ELISA kit to detect the levels of immunoglobulins IgM and IgG. Figure 2 Mice were euthanized by cervical dislocation. Following the LDH assay method disclosed in the literature (Yu Ping et al. Study on the enhancement of mouse immune function by ginseng glycopeptide complexes of different molecular weights [J]. Food and Fermentation Industries, 2021, 47), the absorbance values ​​of each group were detected using an enzyme-linked immunosorbent assay (ELISA) instrument, and the NK cell activity percentage was calculated. Figure 2 The measurement results are taken as the average value (x). - ±s).

[0089] Depend on Figure 2 It is evident that the health supplements prepared in Examples 3-5 all increased the levels of IgG and IgM in mouse serum and enhanced the activity of mouse NK cells, with the health supplement prepared in Example 5 showing the best effect. Examples 5-7 show that the compatibility between Cordyceps militaris polypeptide (CMP2) and the compound enzyme powder of this invention is optimal, with better effects than the other two polypeptides (CMP1 and CMP3). Comparative Examples 1-2 show that, compared to roselle enzyme powder and schisandra enzyme powder, the compound enzyme powder of this invention is more effective in increasing the levels of IgG and IgM in mouse serum and enhancing the activity of mouse NK cells. Comparative Examples 3-4 show that the compound enzyme powder of this invention and Cordyceps militaris polypeptide CMP2 play a synergistic role in increasing the levels of IgG and IgM in mouse serum and enhancing the activity of mouse NK cells.

[0090] Application Example 3: Gastrointestinal Irritation Detection

[0091] Kunming mice weighing 18-22 g were randomly divided into a blank control group, the Example 5 group, and the Comparative Examples 1-2 groups, with 6 mice in each group. Mice in the Example 5 group and the Comparative Examples 1-2 groups were administered the health supplements prepared in Example 5 and Comparative Examples 1-2 by gavage at a dose of 1 g / kg body weight (bw). Mice in the blank control group were administered an equal volume of physiological saline by gavage. After administration, each mouse was placed individually in a beaker lined with filter paper and observed for 6 hours. The number of times the mice experienced diarrhea was recorded. The results were taken as averages (x ± s), and the results are shown in Table 1.

[0092] Table 1 Results of Gastrointestinal Irritation Tests

[0093] Group Number of diarrhea Blank control group 0 Example 5 group 0 Comparative Example 1 3.47±1.29 Comparative Example 2 0

[0094] As shown in the table above, mice fed with health supplements containing compound enzyme powder or Schisandra chinensis enzyme powder (Example 5, Comparative Example 2) did not experience diarrhea, while mice fed with health supplements containing roselle enzyme powder (Comparative Example 1) experienced diarrhea. This result indicates that Schisandra chinensis can effectively inhibit the gastrointestinal irritation of roselle.

[0095] The above are merely preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be considered as limitations on the present invention, and the scope of protection of the present invention should be determined by the scope defined in the claims. For those skilled in the art, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A health product that can relieve fatigue and enhance immunity, characterized in that, The preparation materials include the following parts by weight: 15-25 parts of compound enzyme powder, 5-10 parts of Cordyceps militaris polypeptide, 1-3 parts of stevioside and 15-20 parts of maltodextrin; The preparation steps of the compound enzyme powder are as follows: (1) After soaking dried, mold-free roselle in water, extract it by hot water, filter it, concentrate and dry the filtrate to obtain roselle extract; (2) Wash the Schisandra chinensis clean, dry it and crush it. Add an appropriate amount of water and reflux to extract it. Filter it and concentrate and dry the filtrate to obtain Schisandra chinensis extract. (3) Dissolve roselle extract, schisandra extract and glucose in water, stir and mix well, and pasteurize to obtain fermentation medium; (4) Inoculate Lactobacillus rhamnosus into a fermentation medium for fermentation, centrifuge the fermentation product, collect the supernatant and dry it to obtain compound enzyme powder, wherein Lactobacillus rhamnosus is Lactobacillus rhamnosus NJ551; The mass ratio of roselle extract, schisandra extract, glucose and water is 5:5:(3~4):100; The preparation steps of the Cordyceps militaris polypeptide are as follows: (1) The selenium-enriched Cordyceps militaris powder was extracted with a 5% acetic acid solution containing 0.1% β-mercaptoethanol. After centrifugation, the supernatant was collected and precipitated with saturated ammonium sulfate. After centrifugation, the precipitate was collected and redissolved with pure water. After dialysis and drying, the selenium protein powder was obtained. (2) Dissolve the selenoprotein dry powder in water, adjust the pH to 1.5~3, add pepsin for enzymatic hydrolysis, then adjust the pH to 7.8~8.5, add trypsin for further enzymatic hydrolysis, after inactivation of enzyme, filter, separate the filtrate with an ultrafiltration membrane with a molecular weight cutoff of 5 kDa~10 kDa, collect the components with molecular weights between 10 kDa and 5 kDa, dry, and obtain Cordyceps militaris polypeptide.

2. The health product for relieving fatigue and enhancing immunity according to claim 1, characterized in that, The preparation materials include the following parts by weight: 20 parts of compound enzyme powder, 8 parts of Cordyceps militaris polypeptide, 2 parts of stevioside and 15 parts of maltodextrin.

3. The health product for relieving fatigue and enhancing immunity according to claim 1, characterized in that, The viable count of the Lactobacillus rhamnosus is 10. 9 ~10 10 The inoculum size was CFU / mL, and the inoculum size of Lactobacillus rhamnosus was 3% by volume.

4. The health product for relieving fatigue and enhancing immunity according to claim 1 or 2, characterized in that, The health products mentioned are capsules or tablets.

5. A method for preparing the health product for relieving fatigue and enhancing immunity as described in claim 1 or 2, characterized in that, The process includes the following steps: mixing the formulated amounts of compound enzyme powder, cordyceps militaris polypeptide, stevioside, and maltodextrin, filling the mixture into capsule shells to obtain a capsule-form health product, or adding an appropriate amount of tablet excipients and compressing the mixture into tablets to obtain a tablet-form health product.

6. The preparation method according to claim 5, characterized in that, The tablet excipients include fillers, disintegrants, flow aids, and binders.

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