一种革兰氏阴性菌病原体qPCR检测试剂盒及检测方法

By designing the structure of a qPCR detection kit for Gram-negative bacterial pathogens, the problem of poor reagent bottle stability was solved, and the stability of the reagent tubes during transportation and the reliability of detection were achieved.

CN119953689BActive Publication Date: 2026-07-17THE SEVENTH MEDICAL CENTER OF PLA GENERAL HOSPITAL +1

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE SEVENTH MEDICAL CENTER OF PLA GENERAL HOSPITAL
Filing Date
2025-02-10
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing qPCR detection kits for Gram-negative bacterial pathogens have poor reagent bottle stability, making them prone to tipping and spillage, resulting in reagent solution loss.

Method used

A qPCR detection kit for Gram-negative bacterial pathogens has been designed, comprising a box body, a lid, a placement frame, and reagent tubes. Through structures such as insertion holes, plug rods, rotating frames, U-shaped frames, and clamping components, the stability of the reagent tubes during placement and transportation is ensured, preventing tipping and spillage.

Benefits of technology

It effectively prevents reagent tubes from tilting and colliding within the placement frame, ensuring the stability of the reagent tubes during transportation, avoiding loss of reagent solution, and guaranteeing the accuracy and integrity of the test.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明属于基因检测技术领域,尤其公开了一种革兰氏阴性菌病原体qPCR检测试剂盒及检测方法,该革兰氏阴性菌病原体qPCR检测试剂盒包括盒体,以及盖放在盒体顶部开口处的盒盖,所述盒体内部设置有放置框,所述放置框表面设置有多个插接孔,插接孔内部竖直插接有试剂管,试剂管由透明材料设置,通过将病原体添加到试剂管内部,试剂管内部竖直插接有塞杆。本发明通过将试剂管插接在插接孔内部,两个L型板使得试剂管竖直下移,避免试剂管在放置框内侧倾斜,且试剂管在插接过程中,弹簧的弹力通过弧形板施加在试剂管,两个弧形板的配合夹持,使得试剂管稳定的处于两个L型板之间,保证试剂管在放置框上的稳定性。
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