Method for regulating energy metabolism in plants and use thereof

Abstract

The present application relates to the technical field of plant electric response, in particular to a method for regulating plant energy metabolism and application thereof, the present application regulates plant energy metabolism by using electric bionasty of plants, realizes directional regulation of plant energy metabolism, improves plant energy use efficiency and growth and development, and solves the problem that the prior art cannot regulate plant energy metabolism directionally.

Description

Technical Field

[0001] This invention relates to the field of plant electro-response technology, specifically to a method for regulating plant energy metabolism and its application. Background Technology

[0002] Glycolysis and the tricarboxylic acid (TCA) cycle are important energy metabolism pathways in plant cells. Regulating these pathways can control biological functions and metabolism, improve energy utilization efficiency, and promote plant growth and development. However, currently, there is no method for targeted regulation of plant glycolysis and the TCA cycle. Summary of the Invention

[0003] The purpose of this invention is to provide a method for regulating plant energy metabolism and its application.

[0004] The technical solution is as follows:

[0005] This invention proposes a method for regulating plant energy metabolism by utilizing the plant's electrobipotency.

[0006] Furthermore, plant energy metabolism can be regulated by controlling the direction of plant roots toward the cathode and anode.

[0007] Furthermore, the plants are cultured in a closed-loop system with a culture medium, and the voltage and current are adjusted to determine the voltage and current ranges when the plant roots tend to the cathode and anode, respectively. The root electrotaxis is then regulated according to the determined voltage and current ranges to achieve directional control of plant energy metabolism.

[0008] Furthermore, in step S1, two metal plates are inserted parallel to each other on both sides of the rectangular water tank. The two metal plates are connected to the positive and negative terminals of the DC power supply, respectively. A culture medium is added to the water tank to form a closed loop. The seeds are placed on a sponge and covered with double gauze. Then the sponge is placed in the culture medium in the water tank to cultivate the seeds.

[0009] S2, by adjusting the voltage intensity applied to both sides of the culture medium and the current intensity flowing through the culture medium, the voltage and current ranges when the plant roots tend towards the cathode and anode are determined respectively;

[0010] S3 utilizes voltage and current that exhibit anodic or cathodic tendencies in plant roots to systematically and directionally regulate plant energy metabolism, depending on the needs.

[0011] The present invention also includes applications of any of the methods described above, characterized in that: applications in the fields of biology and crop science.

[0012] Compared with existing technologies, this invention utilizes the electrobipotency of plants to regulate plant energy metabolism, thereby achieving targeted regulation of plant energy metabolism, improving plant energy utilization efficiency and growth and development, and solving the problem that existing technologies cannot target and regulate plant energy metabolism.

[0013] Instruction manual illustrations

[0014] Figure 1 This is a diagram of the experimental setup used in one embodiment.

[0015] Figure 2 This is a diagram illustrating the electrotaxis results of plant roots under different currents in one embodiment. Detailed Implementation

[0016] The technical solution of the present invention will be further described below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanatory of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0017] Example 1

[0018] S1. Two stainless steel plates are inserted parallel to each other on both sides of a rectangular transparent organic plate water tank. The two stainless steel plates are connected to the positive and negative terminals of a DC power supply, respectively. Distilled water is added to the water tank to form a closed loop. The seeds are placed on a sponge and covered with double layers of gauze. Then the sponge is placed in the distilled water in the water tank to cultivate the seeds.

[0019] S2, by adjusting the voltage intensity applied to both sides of the culture medium, determines the range of cathodic directional voltage exhibited by the roots grown from the seed;

[0020] S3. Select any voltage value within the above voltage range. Under this voltage value, change the conductivity of the culture medium to adjust the current intensity and determine the current range A1 and the current range A2 where the roots grown from the seed exhibit a tendency towards anodic growth.

[0021] S4 utilizes voltage and current that exhibit anodic or cathodic tendencies in plant roots to systematically and directionally regulate plant energy metabolism, depending on the needs.

[0022] Example 2

[0023] (1) See Figure 1 Wheat (Yanong 19) seeds were evenly placed on six 2cm thick sponges, with a 1cm gap between adjacent seeds. The sponges were covered with double layers of gauze and placed in six water troughs (30×10×10cm). Each of the six troughs contained 1L of distilled water as the culture medium.

[0024] (2) Two stainless steel plates (10×10×0.1cm) were inserted parallel to each end of each water tank and then connected to the anode and cathode of the electrophoresis apparatus, respectively. The voltage of the water tank was controlled by the electrophoresis apparatus. The set voltages of the five water tanks were 1V, 5V, 10V, 20V and 30V, respectively. The remaining treatment group was not powered on and served as a control.

[0025] (3) After 6 days of dark incubation at 20±0.1℃, record the root tropism:

[0026] In the control group, the number of roots growing towards the cathode and anode were equal. When the applied voltage was 1V, root growth direction was unaffected; when the applied voltage was 5V, 80% of roots were observed to grow towards the cathode; when the applied voltage was 10V, all roots were observed to grow towards the cathode; when the applied voltage was 20V, all roots were observed to grow towards the cathode, but root growth was significantly inhibited; when the applied voltage was 30V, root growth was severely inhibited and unable to occur normally. Therefore, the voltage conditions for wheat roots to exhibit cathodic tendency behavior are 5–20V.

[0027] Example 3

[0028] (1) Wheat (Yanong 19) seeds were evenly placed on 6 sponges 2cm thick, with a 1cm gap between adjacent seeds. The sponges were covered with double-layered gauze and placed in 6 water tanks (30×10×10cm). By changing the ratio of distilled water and tap water, mixtures with different conductivity were prepared. The culture media in the 6 water tanks were 1L of distilled water, distilled water, a mixture of distilled water and tap water 1, a mixture of distilled water and tap water 2, a mixture of distilled water and tap water 3, and tap water.

[0029] (2) Two stainless steel plates (10×10×0.1cm) were inserted parallel to each end of each water tank and then connected to the anode and cathode of the electrophoresis apparatus, respectively. One treatment group, which only added distilled water, was not powered on and served as a control. The other five groups were all powered by a voltage of 10V and current intensities of 0.005mA, 0.3mA, 0.5mA, 1mA, and 2mA, respectively.

[0030] (3) After culturing in the dark at 20±0.1℃ for 6 days, take photos and record the root tropism.

[0031] The results showed that the number of growths in the control group was the same in both the cathode and anode directions; see reference. Figure 2In the treatment group with a current intensity of 0.005 mA, all roots were observed to grow towards the cathode; in the treatment group with a current intensity of 0.3 mA, 60% of the roots were observed to grow towards the cathode; in the treatment group with a current intensity of 0.5 mA, 80% of the roots were observed to grow towards the anode; in the treatment group with a current intensity of 1 mA, all roots were observed to grow towards the anode; and in the treatment group with a current intensity of 2 mA, all roots were observed to grow towards the anode, and root growth was significantly inhibited. Therefore, the current conditions for wheat roots to exhibit cathodic tendency behavior are greater than 0–0.3 mA (excluding 0), and the current conditions for exhibiting anodic tendency behavior are 0.5–2 mA.

[0032] Example 4

[0033] The following steps are used to regulate energy metabolism based on the electroresponsive characteristics of wheat roots exhibiting electrobipotency:

[0034] Wheat (Yanong 19) seeds were evenly placed on four 2cm thick sponges, with adjacent seeds spaced 1cm apart. The sponges were covered with double layers of gauze and placed in four 30×10×10cm water tanks. Two tanks contained 1L of distilled water as the culture medium, while the other two contained 1L of tap water. Two stainless steel plates (10×10×0.1cm) were inserted parallel to each end of each tank and then connected to the anode and cathode of the electrophoresis apparatus, respectively. The tank voltage was controlled by the electrophoresis apparatus; one tank each of tap water and distilled water was subjected to a 10V voltage, while the remaining tank served as a control without voltage. After 6 days of dark incubation at 20±0.1℃, root samples were collected for metabolomics analysis. This experiment was repeated six times, resulting in six batches of samples.

[0035] For metabolomics, refer to Table 1 below. When a voltage of 10V and a current of 0.005mA are applied to wheat, the content of pyruvate, an important metabolite in the root glycolysis pathway, decreases compared with the control group, indicating that the metabolic process of the glycolysis pathway is weakened; the content of malic acid, an important metabolite in the tricarboxylic acid cycle, increases compared with the control group, indicating that the metabolic process of the tricarboxylic acid cycle is enhanced.

[0036] When a voltage of 10V and a current of 2mA were applied to wheat, the content of pyruvate, an important metabolite in the root glycolysis pathway, increased compared with the control group, indicating that the metabolic process of the glycolysis pathway was enhanced; the content of malic acid, an important metabolite in the tricarboxylic acid cycle, increased compared with the control group, indicating that the metabolic process of the tricarboxylic acid cycle was enhanced.

[0037] The above results indicate that the exhibited electrobipotency of organisms can be used to directionally regulate the energy metabolism of plants.

[0038] Table 1 shows the effects of 10V voltage, 0.005mA current, and 2mA current on the glycolytic pathway and the tricarboxylic acid cycle.

[0039]

[0040] While specific embodiments of the present invention have been described above, those skilled in the art should understand that the specific embodiments described are merely illustrative and not intended to limit the scope of the invention. Modifications and variations made by those skilled in the art in accordance with the spirit of the invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for regulating plant energy metabolism, characterized in that: Utilizing the electrobipotency of plants to regulate plant energy metabolism; Plant energy metabolism is regulated by controlling the direction of plant roots toward the cathode and anode. Plants are cultured in a closed-loop system with a culture medium. Voltage and current are adjusted to determine the voltage and current ranges when plant roots tend to the cathode and anode, respectively. The root electrotaxis is regulated according to the determined voltage and current ranges to achieve directional control of plant energy metabolism. S1, two metal plates are inserted parallel to each other on both sides of the rectangular water tank. The two metal plates are connected to the positive and negative terminals of the DC power supply, respectively. A culture medium is added to the water tank to form a closed loop. The seeds are placed on a sponge and covered with double gauze. Then the sponge is placed in the culture medium in the water tank to cultivate the seeds. S2, by adjusting the voltage intensity applied to both sides of the culture medium and the current intensity flowing through the culture medium, the voltage and current ranges when the plant roots tend towards the cathode and anode are determined respectively; S3 utilizes voltage and current that exhibit anodic or cathodic tendencies in plant roots to systematically and directionally regulate plant energy metabolism, depending on the needs.

2. The application of the method according to claim 1, characterized in that: Applications in the fields of biology and crop science.

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