Recombinant 17-type collagen with transdermal effect as well as preparation method and application of recombinant 17-type collagen

Through genetic engineering technology and biofermentation technology, recombinant 17 type collagen with histidine tag is designed and expressed, and its transdermal effect is enhanced by adding the membrane-penetrating peptide Tat, which solves the problems of unstable quality, safety hazards and poor biocompatibility in the existing 17 type collagen acquisition methods, and achieves efficient and safe product production and widespread clinical applications.

CN119978110AActive Publication Date: 2025-05-13GUANGZHOU JIYUAN BIOLOGICAL TECH CO LTD
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Patent Information

Application Number
CN202510464774.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-15
Publication Date
2025-05-13
Estimated Expiration
2045-04-15

AI Technical Summary

Technical Problem

The existing method for obtaining type 17 collagen has problems such as unstable quality, safety hazards and poor biocompatibility, which limits its widespread use in clinical applications.

Method used

Through genetic engineering and biofermentation technology, recombinant 17 collagen with histidine tag is designed and expressed, which can achieve its efficient purification and industrial production, and enhance its transdermal effect by adding the membrane-transplant peptide Tat.

Benefits of technology

It has achieved efficient expression and simple purification of recombinant 17 collagen, ensuring the consistency and stability of the product, improving safety and biocompatibility, enhancing its transdermal effect, and expanding its application potential in the fields of skin repair, anti-aging, etc.

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Abstract

The invention particularly relates to recombinant 17-type collagen with a transdermal effect as well as a preparation method and application of the recombinant 17-type collagen. The amino acid sequence of the recombinant 17-type collagen provided by the invention is selected from one of SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25 and SEQ ID NO. 26. The recombinant 17-type collagen provided by the invention is a recombinant 17-type collagen. The recombinant protein finally obtained by analyzing the fragments of the natural 17-type collagen, screening out the fragments of a plurality of active sites and further splicing and modifying has good biological activity and transdermal effect, the expression of the soluble target protein is realized, the later purification is simple and convenient, the industrial production requirement is met, and the method is suitable for industrial production. And a foundation is laid for further expanding the application of the recombinant collagen.
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Citation Information

Patent Citations

  • Human collagen 17-type polypeptide and production method and application thereof

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  • Recombinant human XVII type collagen, and preparation method and application thereof

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  • Preparation method of recombinant humanized collagen for improving skin repair function

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  • Recombinant human XVII type collagen as well as preparation method and application thereof

    CN119219762A

  • High-activity recombinant human XVII type collagen as well as preparation method and application thereof

    CN119462899A

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