A recombinant foot-and-mouth disease virus strain with a mutated l protein and use in vaccine preparation
CN119979606BActive Publication Date: 2026-08-28LANZHOU VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES(LANZHOU BRANCH CENTER OF CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENTER)
Patent Information
- Application Number
- CN202510187991.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-20
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2045-02-20
AI Technical Summary
Benefits of technology
[0036]有益效果：本发明首先发现在宿主细胞中过表达CISD2蛋白会抑制FMDV的复制，并且解析了抑制机制，具体包括CISD2通过抑制L蛋白的表达，尤其是通过蛋白酶体途径引起L蛋白的降解，进而抑制FMDV复制。本发明还鉴定出了CISD2蛋白降解L蛋白的关键位点，利用反向遗传技术，通过在L蛋白中引入关键位点氨基酸突变(18位和28位氨基酸序列)，成功构建并拯救得到L蛋白关键氨基酸位点突变的重组口蹄疫病毒株，消除了CISD2蛋白降解L蛋白及抑制FMDV复制的能力，可用于制备重组口蹄疫病毒株及重组疫苗株。
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Abstract
The application provides a L protein mutant recombinant foot-and-mouth disease virus strain and application in vaccine preparation, and belongs to the technical field of biological medicine. The application first finds that overexpression of CISD2 protein in a host cell can inhibit replication of FMDV, and analyzes the inhibition mechanism, and specifically comprises that CISD2 causes degradation of L protein through inhibition of expression of the L protein, especially through a proteasome pathway, and then inhibits FMDV replication. The application further identifies a key site of the CISD2 protein for degrading the L protein, and by introducing a key site amino acid mutation in the L protein through reverse genetic technology, successfully constructs and rescues a recombinant foot-and-mouth disease virus with the L protein key amino acid site mutation, eliminates the ability of the CISD2 protein for degrading the L protein and inhibiting FMDV replication, improves a virus titer, has good production performance, and can be used for preparing a recombinant foot-and-mouth disease virus strain and a recombinant vaccine strain.
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Citation Information
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