Semi-full-automatic device for soybean transgenic test paper detection

Abstract

The invention discloses a semi-automatic soybean transgenic test paper detection device. The device is composed of a conveying crawler belt, a container, a quantitative water injection device, a pulverizer, a magnetic stirring device, a high-pressure cup washing device, a filter screen, a water feeding and discharging device and a water tank located below the conveying crawler belt. Wherein the eight sets of containers are arranged in total, and the containers are evenly clamped to the upper surface of the conveying crawler belt at equal intervals; the semi-automatic soybean transgene test paper detection device provided by the invention adopts a plurality of structures such as the conveying crawler belt, the container, the quantitative water outlet device, the crusher, the magnetic stirring device, the high-pressure cup washing device, the filter screen and the water feeding and discharging device, and can realize an automatic soybean transgene detection process through the structures; compared with a manual detection process in the prior art, the detection device has the advantages that the detection efficiency of soybean transgenes can be improved, manpower is greatly saved, and the working process is optimized.

Description

Technical Field

[0001] The invention relates to the technical field of soybean genetically modified test paper detection, and in particular to a semi-automatic device for soybean genetically modified test paper detection. Background Art

[0002] Genetically modified corn and soybeans are currently the world's main genetically modified food crops. Genetically modified soybeans are the earliest commercially grown and fastest-growing genetically modified food crops. Since they were approved for commercial production in 1996, the planting area has reached 65.8 million hectares by 2008, accounting for 69% of the world's total soybean planting area and 53% of the world's genetically modified crops. As genetically modified products emerge in large numbers in the global market, their environmental and food safety issues have also attracted high international attention.

[0003] Due to the requirements for domestic soybeans to be put into storage, GMO testing is carried out on each truck. Domestic soybean storage companies have a heavy workload. It is of great significance to reduce the work pressure of employees, reduce labor costs and improve testing efficiency.

[0004] The conventional qualitative PCR method has low detection efficiency and is time-consuming and labor-intensive when used to detect soybean GMOs, and cannot meet people's needs for detecting soybean GMOs.

[0005] Therefore, a semi-automatic device for detecting soybean GMO test strips has become an urgent problem to be solved by the entire society. Summary of the invention

[0006] In order to solve the above technical problems, the technical solution provided by the present invention is: a semi-automatic device for soybean genetically modified test paper detection, comprising a conveyor belt, a container, a quantitative water injection device, a crusher, a magnetic stirring device, a high-pressure cup washer, a filter, a water supply and discharge device, and a water tank located below the conveyor belt;

[0007] There are 8 groups of containers in total, and the containers are evenly clamped on the upper surface of the conveyor belt at equal distances;

[0008] The conveyor belt is driven to move by a driving device, which includes a driving motor and a plurality of driving rollers. Both ends of the driving rollers are fixed with transmission gears meshing with the inner tooth grooves of the conveyor belt, and the output shaft of the driving motor is connected to one end of the driving roller through a coupling, so as to drive the conveyor belt to operate and drive the container on the upper surface of the conveyor belt to move;

[0009] The pulverizer is provided with a pulverizing chamber and a pulverizing motor installed in the pulverizing chamber, the output shaft of the pulverizing motor is connected to a plurality of pulverizing blades through a coupling, and is used to pulverize the soybean material in the pulverizing chamber, an end cover is installed on the front end surface of the pulverizing chamber through a reset hinge, and a strip-shaped through hole is provided inside the end cover for the pulverizing motor to pass through;

[0010] The quantitative water injection device comprises a water tank, a water pipe, and a water pump. The water pump is installed above the water tank, the water injection port is arranged at the top of the water tank, the drainage port of the water pump is connected to the water pipe through a pipe joint, the water pump is used to quantitatively pump water and transport it to the water pipe, and the water outlet of the water pipe is located above the container;

[0011] The magnetic stirring device comprises a magnetic coil and magnetic stirring particles, the magnetic stirring particles are arranged in a container, the magnetic coil is used to drive the magnetic stirring particles to achieve stirring, and the magnetic coil is located inside the conveyor belt;

[0012] A test paper pushing and releasing device is arranged at the top of the container, and the test paper pushing and releasing device comprises a test paper, a porous test paper circle, a lower-layer covering acrylic plate and a servo motor. The output shaft of the servo motor is fixedly connected to the upper end surface of the porous test paper circle. The test paper is arranged in the porous test paper circle. An opening that coincides with the internal holes of the porous test paper circle is arranged inside the lower-layer covering acrylic plate. When the container moves to the bottom of the test paper pushing and releasing device, the lower-layer covering acrylic plate is pushed aside to allow the test paper to fall from the porous test paper circle into the container, and soybean genetic modification detection is achieved through the test paper inside the container.

[0013] Furthermore, the capacity of each group of containers is the same, and from left to right they are a crushing and feeding container, a water injection container, a stirring container, a test paper detection container, a rinsing container, a first rinsing container, a second rinsing container and a third rinsing container. The filter is installed between the crushing and feeding container, the water injection container and the water pool to recover most of the residue.

[0014] Furthermore, the filter is a pull-out filter.

[0015] The advantages of the invention compared with the prior art are:

[0016] The present invention adopts multiple structures such as a conveyor belt, a container, a quantitative water injection device, a pulverizer, a magnetic stirring device, a high-pressure cup washer, a filter screen and a water supply and drainage device to provide a semi-automatic device for soybean genetically modified test paper detection. The above structure can realize the automatic detection process of soybean genetically modified. Compared with the manual detection process in the prior art, the above detection device can improve the detection efficiency of soybean genetically modified, greatly save manpower, and optimize the work process. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of an automatic soybean genetically modified test paper detection device;

[0018] Figure 2 is a schematic diagram of the water tank;

[0019] Figure 3 is a schematic diagram of the crushing mechanism;

[0020] Figure 4 It is a schematic diagram of the water injection mechanism;

[0021] Figure 5 It is a schematic diagram of the test paper delivery mechanism;

[0022] Figure 6 is a schematic diagram of a crawler mechanism;

[0023] Figure 7 A schematic diagram of a container.

[0024] Among them, 1. conveyor belt, 2. container, 3. quantitative water injection device, 4. crusher, 5. magnetic stirring device, 51. magnetic coil, 52. magnetic stirring particles, 6. high-pressure cup washer, 7. filter, 8. water supply and drainage device, 9. sink, 10. sewer pipe, 11. test paper pushing and releasing device, 1101. porous test paper ring, 1102. test paper, 1103. lower layer covering acrylic plate, 1104. servo motor, 1105. opening, 2. support plate, 13. crushing blade, 14. crushing chamber, 15. crushing motor, 16. water tank, 17. water pipe, 18. water pump, 19. driving roller, 20. driving motor, 21. timing device, 22. test paper delivery slot; 23. fine hole, 24. end cover. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0026] The present invention is described in detail with reference to the accompanying drawings.

[0027] The present invention provides a semi-automatic device for detecting soybean genetically modified test strips in a specific implementation, comprising a conveyor belt 1, a container 2, a quantitative water injection device 3, a crusher 4, a magnetic stirring device 5, a high-pressure cup washer 6, a filter screen 7, a water supply and discharge device 8, and a water tank 9 located below the conveyor belt 1;

[0028] There are 8 groups of containers 2, and the containers 2 are evenly clamped on the upper surface of the conveyor belt 1 at equal distances;

[0029] The conveyor belt 1 is driven to move by a driving device, which includes a driving motor 20 and a plurality of driving rollers 19. Both ends of the driving rollers 19 are fixed with transmission gears meshing with the inner tooth grooves of the conveyor belt 1, and the output shaft of the driving motor 20 is connected to one end of the driving roller through a coupling, so as to drive the conveyor belt 1 to operate and drive the container 2 on the upper surface of the conveyor belt 1 to move;

[0030] The pulverizer 4 is provided with a pulverizing chamber 14 and a pulverizing motor 15 installed in the pulverizing chamber 14. The output shaft of the pulverizing motor 15 is connected to a plurality of pulverizing blades 13 through a coupling, and is used to pulverize the soybean material in the pulverizing chamber 14. An end cover 24 is installed on the front end surface of the pulverizing chamber 14 through a reset hinge, and a strip-shaped through hole is provided inside the end cover 24 for the pulverizing motor 15 to pass through.

[0031] The quantitative water injection device 3 includes a water tank 16, a water pipe 17, and a water pump 18. The water pump 18 is installed above the water tank 16, and the water injection port is arranged at the top of the water tank 16. The drainage port of the water pump 18 is connected to the water pipe 17 through a pipe joint. The water pump 18 is used to quantitatively pump water and transport it to the water pipe 17. The water outlet of the water pipe 17 is located above the container 2.

[0032] The magnetic stirring device 5 comprises a magnetic coil 51 and magnetic stirring particles 52. The magnetic stirring particles 52 are arranged in the container 2. The magnetic coil 51 is used to drive the magnetic stirring particles to achieve stirring, and the magnetic coil 51 is located inside the conveyor belt 1.

[0033] A test paper pushing and releasing device 11 is provided at the top of the container 2, and the test paper pushing and releasing device 11 includes a test paper 1102, a porous test paper circle 1101, a lower covering acrylic plate 1103 and a servo motor 1104. The output shaft of the servo motor 1104 is fixedly connected to the upper end surface of the porous test paper circle 1101. The test paper 1102 is arranged in the porous test paper circle 1101, and an opening 1105 that coincides with the internal holes of the porous test paper circle 1101 is provided inside the lower covering acrylic plate 1103. When the container 2 moves to the bottom of the test paper pushing and releasing device, the lower covering acrylic plate 1103 is pushed aside to allow the test paper 1102 to fall from the porous test paper circle 1101 into the container 2, and soybean genetic modification detection is achieved through the test paper 1102 inside the container 2.

[0034] Example:

[0035] The outer surface of the conveyor belt 1 is provided with a clamp for clamping the bottom of the container 2 at an even distance, wherein the clamp may be a ring made of silicone or rubber, the inner diameter of the ring being consistent with the bottom diameter of the container 2, and being used for the cup mouth of the container 2 to face the flushing port of the high-pressure cup washer 6 when the transmission belt 1 moves the container 2 to the bottom, while keeping it in a non-falling state; when the device is working, 8 identical containers 2 are distributed on the conveyor belt 1, and the containers 2 are arranged in eight groups, each group of containers 2 has the same capacity, from left to right, they are a crushing and unloading container, a water injection container, a stirring container, a test paper detection container, a flushing container, a first flushing container, a second flushing container and a third flushing container, and one container position is controlled to rotate every three minutes, wherein the specific control process of the container movement is as follows: the corresponding gear is driven to rotate by the driving motor 20, and the gear drives the container 2 fixed on the conveyor belt to move.

[0036] When the equipment is started, it starts working according to the order of the containers. The quantitative samples are put into the crusher and crushed into the container. At the same time, water is added, stirred, and tested. After the fourth sample is crushed, the cycle officially begins. After testing, it is transferred to the lower layer for 4 rinses to ensure that it is clean. Single cycle instructions are issued by the timing / timing / counting device, every 3 minutes / manually issued (3 minutes is the test paper test time). Once an instruction is issued, the counting device counts 1 time, the timing alarm is set and the timing is reset. The equipment must be equipped with water supply and drainage and corresponding sinks 9.

[0037] The container 2 needs to be customized with a filter screen 7 with a fixed water level on the inner side of the cup wall to receive the test paper push-and-release device. The material of the container 2 should be such that the magnetic stirring rotor 52 will not fall off after being inverted. The filter screen 7 is installed between the crushing and feeding container, the water injection container and the water pool to recover most of the residue.

[0038] The staff can put the soybean material into the feed port, and after the equipment is started, the crushing motor 15 drives the crushing components in the crushing chamber 14 to crush the soybean. The pumping time of the pumping pump 18 can be controlled by PLC control to control the rated pumping amount.

[0039] When the conveyor belt 1 is in operation, when the container 2 is moved under the magnetic coil 51, the magnetic coil 51 starts to work, and the magnetic coil 51 drives the magnetic stirring particles 52 in the container 2 to fully blend the soybeans and water. When the magnetic coil 51 is placed in the container 2 at the third cup position and the main control unit starts to operate, the equipment runs for 10 seconds to fully stir the materials.

[0040] A test paper pushing device 11 is provided at the top of the container 2, and the test paper pushing device 11 includes a test paper 1102, a porous test paper circle 1101 and a lower covering acrylic plate 1103. The test paper 1102 is arranged in the porous test paper circle 1101. When the container 2 moves to the bottom of the test paper pushing device, the test paper 1102 falls from the porous test paper circle 1101 into the container 2. The test paper 1102 contacts with the soybean material after crushing and water injection inside the container 2 for detection. The above structure can realize the automatic detection of soybean genes.

[0041] The container 2 is an iron cup structure. When the container 2 reaches the fourth cup position, the mechanical clock counts and starts a 3-minute countdown. After the countdown ends, the buzzer sounds.

[0042] As a further elaboration of the present invention, the filter screen 7 is a pull-out filter screen.

[0043] In order to achieve precise control of the driving motor 20 and the water pump 18, the driving motor 20 and the water pump 18 are both controlled by a PLC controller.

[0044] As a further explanation of the present invention, the high-pressure cup washer 6 is arranged below the container 2, a water tank 9 is arranged around the high-pressure cup washer 6, and a downpipe 10 is arranged at the bottom of the water tank 9. The above structure can realize high-pressure cleaning of the container 2 and clean the container.

[0045] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. A semi-automatic device for soybean genetically modified test paper detection, characterized in that: It comprises a conveyor belt (1), a container (2), a quantitative water injection device (3), a crusher (4), a magnetic stirring device (5), a high-pressure cup washer (6), a filter (7), a water inlet and outlet device (8), and a water tank (9) located below the conveyor belt (1); There are 8 groups of containers (2) in total, and the containers (2) are evenly clamped on the upper surface of the conveyor belt (1) at equal distances; The conveyor belt (1) is driven to move by a driving device, the driving device comprising a driving motor (20) and a plurality of driving rollers (19), both ends of the driving rollers (19) are fixed with transmission gears meshing with the inner tooth grooves of the conveyor belt (1), and the output shaft of the driving motor (20) is connected to one end of the driving roller through a coupling, so as to drive the conveyor belt (1) to operate and drive the container (2) on the upper surface of the conveyor belt (1) to move; The pulverizer (4) is provided with a pulverizing chamber (14) and a pulverizing motor (15) installed in the pulverizing chamber (14); a plurality of pulverizing blades (13) are connected to the output shaft of the pulverizing motor (15) via a coupling, and are used to pulverize the soybean material in the pulverizing chamber (14); an end cover (24) is installed on the front end surface of the pulverizing chamber (14) via a reset hinge; a strip-shaped through hole is provided inside the end cover (24) for the pulverizing motor (15) to pass through; The quantitative water injection device (3) comprises a water tank (16), a water pipe (17), and a water pump (18); the water pump (18) is installed above the water tank (16); the water injection port is arranged at the top of the water tank (16); the drainage port of the water pump (18) is connected to the water pipe (17) through a pipe joint; the water pump (18) is used to quantitatively pump water and transport it to the water pipe (17); the water outlet of the water pipe (17) is located above the container (2); The magnetic stirring device (5) comprises a magnetic coil (51) and magnetic stirring particles (52), wherein the magnetic stirring particles (52) are arranged in the container (2), and the magnetic coil (51) is used to drive the magnetic stirring particles to achieve stirring, and the magnetic coil (51) is located inside the conveyor belt (1); A test paper pushing and placing device (11) is arranged at the top of the container (2), and the test paper pushing and placing device (11) comprises a test paper (1102), a porous test paper circle (1101), a lower covering acrylic plate (1103), and a servo motor (1104), wherein the output shaft of the servo motor (1104) is fixedly connected to the upper end surface of the porous test paper circle (1101), and the test paper (1102) is arranged in the porous test paper circle (1101). The lower acrylic plate (1103) is provided with an opening (1105) which coincides with the holes inside the porous test paper circle (1101). When the container (2) is moved to the bottom of the test paper pushing and releasing device, the lower acrylic plate (1103) is pushed aside to allow the test paper (1102) to fall from the porous test paper circle (1101) into the container (2), and the test paper (1102) inside the container (2) is used to detect the genetically modified soybeans.

2. A semi-automatic soybean genetically modified test paper detection device according to claim 1, characterized in that: Each group of containers (2) has the same capacity, and from left to right are a crushing and discharging container, a water injection container, a stirring container, a test paper detection container, a flushing container, a first flushing container, a second flushing container and a third flushing container. The filter (7) is installed between the crushing and discharging container, the water injection container and the water pool to recover most of the residue.

3. A semi-automatic soybean genetically modified test paper detection device according to claim 2, characterized in that: The filter screen (7) is a pull-out filter screen.

4. A semi-automatic soybean genetically modified test paper detection device according to claim 1, characterized in that: The driving motor (20) and the water pump (28) are both controlled by a PLC controller.

5. A semi-automatic soybean genetically modified test paper detection device according to claim 1, characterized in that: It also includes a timing device (21), which includes a mechanical clock and a buzzer, wherein the mechanical clock counts, and the buzzer sounds after the timing is completed.

6. A semi-automatic soybean genetically modified test paper detection device according to claim 1, characterized in that: The high-pressure cup washer (6) is arranged below the container (2), the high-pressure cup washer (6) is arranged on the upper surface of the water tank (9), a downpipe (10) is fixed to the top of the water tank (9), and the filter screen (7) is arranged on the right side of the top of the water tank (9).

7. A semi-automatic soybean genetically modified test paper detection device according to claim 1, characterized in that: A test paper delivery slot (22) is fixed on the inner cavity side wall of the container (2), the test paper delivery slot (22) is coaxially arranged with the opening (1105) inside the lower covering acrylic plate (1103), and a fine hole (23) is provided on the side wall of the test paper delivery slot (22) which is lower than the water level line inside the container (2) for allowing the solution in the container (2) to enter and react with the test paper (1102).

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