A desaturase TgDH and its applications

By using the desaturase TgDH and its mutant L338A for whole-cell biocatalysis, the complexity and environmental problems in the synthesis of chiral 4-substituted cyclohexenone compounds were solved, achieving efficient, green, and precise asymmetric synthesis of chiral enone compounds.

CN120005840BActive Publication Date: 2026-05-26NANJING TECH UNIV
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING TECH UNIV
Filing Date
2025-02-21
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing technologies for synthesizing chiral 4-substituted cyclohexenone compounds suffer from problems such as complex synthesis methods, difficulty in controlling stereoselectivity, environmental unfriendliness, and high catalyst costs.

Method used

Using the desaturase TgDH and its mutant L338A, a chiral 4-substituted cyclohexanone compound was catalyzed by a whole-cell biocatalyst under mild conditions to generate asymmetric 4-substituted cyclohexenone. This was achieved by utilizing the high stereoselectivity and high efficiency of the desaturase.

Benefits of technology

The synthesis of chiral enone compounds with high yield and high stereoselectivity was achieved. The catalytic reaction conditions were mild and environmentally friendly. The catalyst was inexpensive and readily available. The separation and purification were simple. The optical purity was greater than 99%. Both the theoretical yield and the actual separation yield were high.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN120005840B_ABST
    Figure CN120005840B_ABST
Patent Text Reader

Abstract

This invention discloses a desaturase TgDH having the amino acid sequence shown in SEQ ID No. 2 or SEQ ID No. 3. Using TgDH as a biocatalyst, this invention enables the high-yield and highly stereoselective synthesis of chiral enone compounds. This enzyme-catalyzed asymmetric route features mild reaction conditions, is environmentally friendly, highly selective, uses readily available and inexpensive catalysts, and is easy to separate and purify, achieving an optical purity greater than 99% ee and a relatively high actual separation yield (77%). This invention also discloses an asymmetric method for the synthesis of chiral 4-substituted cyclohexenone compounds, using 4-substituted cyclohexenone compounds as substrates and TgDH or whole cells containing TgDH as catalysts in a solvent.
Need to check novelty before this filing date? Find Prior Art