Viral short enhancer element for increasing gene transcription level and use thereof

By inserting virus-derived non-palindromic short enhancer elements into the promoter region of plant genes, the insertion limitations and universality issues of gene activation in existing technologies have been resolved, achieving efficient and adjustable gene activation effects applicable to a variety of plants and gene regulation.

CN120005879BActive Publication Date: 2026-07-14SHANGHAI JIAOTONG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI JIAOTONG UNIV
Filing Date
2023-11-16
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

In existing technologies, plant gene activation methods are limited by the size of the inserted open reading frame sequence, use exogenous strong promoters to induce T-DNA insertion, which makes it difficult to accurately activate specific genes and has poor versatility, lacking universal and efficient enhancer elements.

Method used

We provide non-palindromic short enhancer elements (STEs) derived from viruses, with a length of 30-200 bp, which are inserted into specific regions of the target gene promoter to improve gene transcription levels through site-specific insertion. Gene editing is performed using CRISPR/Cas9 technology.

Benefits of technology

It achieves efficient and adjustable gene activation, with activation multiples ranging from tens to thousands of times. It is applicable to a variety of plants, has stable heritability and wide applicability, and can activate single or multiple genes to improve plant resistance to environmental conditions and agronomic traits.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present application provides a virus short enhancer capable of realizing site-specific activation of plant genome, which realizes efficient activation of a gene by directional insertion into a promoter region of the plant gene. Specifically, the present inventors obtain 15 short enhancers from viruses with different activation multiples through a large number of screening, which can realize activation effect in different reporter genes and have universality. The virus enhancer of the present application can be used for efficient and flexible regulation of transcription of complex genes of plants such as rice.
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