A method for preparing concentrated protein from a high-variety oilseed meal

Abstract

The application discloses a method for preparing concentrated protein from high-denatured oilseed meal, and belongs to the technical field of edible protein processing. The method uses the distribution rules and physical property characteristics of the components of the protein and structural carbohydrates in the microstructure of the high-denatured oilseed meal, obtains meal powder with different protein contents through crushing and screening, and classifies and processes the meal powder according to the property differences; uses an air-electric field separation device to process large-particle oilseed meal, separates high-fiber components that are difficult to remove in a traditional process, and obtains high-protein components with similar solubility (NSI) to low-denatured oilseed meal and high gelation; and according to the protein content, denaturation degree and structural characteristics of the screened components, suitable concentration or alcohol enzyme treatment is selected, so that concentrated protein products meeting specific protein content requirements and / or functional characteristics can be obtained.

Description

Technical Field

[0001] This invention belongs to the field of edible protein processing technology, and particularly relates to a method for preparing concentrated protein from highly denatured oilseed meal. Background Technology

[0002] Oilseed meal, a byproduct of vegetable oil processing, is a raw material for processing plant protein. It can be used directly as a feed ingredient or processed into concentrated or isolated protein for food or feed processing. The functional and nutritional properties of protein together determine the uses of concentrated or isolated protein, among which functional properties include solubility, water retention, oil retention, emulsification, and gelling properties.

[0003] Taking soy protein concentrate as an example, the current mainstream process is the alcohol washing method. This involves using low-denatured defatted soybean meal as raw material and dissolving small-molecule impurities in the meal using 65%-70% ethanol. The insoluble protein components are then processed through desolvation, drying, and pulverization to produce soy protein concentrate. Commercially available products have a protein content of over 65% (dry basis). For functional modification, soy protein concentrate can be mixed with water in a certain proportion, and the pH adjusted to alkaline. After micronization, sterilization, and drying, functional soy protein concentrate is obtained. Compared to the alkali dissolution and acid precipitation process for separating proteins, the alcohol washing process for concentrating proteins has a smaller environmental impact.

[0004] Based on differences in soybean oil processing techniques, soybean meal can be divided into low-temperature defatted soybean meal (white soybean flakes) and highly denatured soybean meal (high-temperature meal). Low-temperature defatted soybean meal is obtained by desolventizing soybean meal after oil extraction at low temperature or flash evaporation. Its protein denaturation is relatively small, making it suitable for producing soy protein. Highly denatured soybean meal undergoes conditioning, puffing, grinding, and high-temperature desolventizing, resulting in severe protein denaturation. Using it to produce concentrated soy protein (using an alcohol washing process) is inefficient, produces products with low protein content, low solubility (NSI < 10%), and poor functional properties, making it impractical. Currently, there are no commercially available products using highly denatured soybean meal as a raw material for producing soy protein concentrate and soy protein isolate.

[0005] Therefore, how to efficiently utilize highly denatured oilseed meal to prepare concentrated protein has become a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention proposes a method for preparing concentrated protein from highly denatured oilseed meal.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] This invention provides a method for preparing concentrated protein from highly denatured oilseed meal, comprising the following steps:

[0009] (1) The highly denatured oilseed meal was crushed and screened to obtain large-particle oilseed meal, medium-particle oilseed meal and small-particle oilseed meal respectively.

[0010] (2) The large-particle oilseed meal obtained in step (1) is subjected to air-electric field separation treatment to obtain high-protein component and high-fiber component respectively;

[0011] The high-protein component obtained in step (2) and the small-particle oilseed meal obtained in step (1) are respectively subjected to a first concentration to obtain concentrated protein;

[0012] The medium-particle oilseed meal obtained in step (1) is subjected to alcohol enzyme treatment and second concentration to obtain functional concentrated protein.

[0013] Technical Principle: Utilizing the distribution patterns and physical properties of proteins and structural carbohydrates in the microstructure of oilseed meal, the process first involves crushing and sieving to obtain meal powders with different protein contents. These powders are then graded based on their differences in properties. Large-particle oilseed meal is processed using an air-electric field separation device to separate high-fiber components that are difficult to remove in traditional processes, resulting in high-protein components with solubility (NSI) similar to that of low-denatured oilseed meal and high gelation properties. Based on the protein content, denaturation degree, and structural characteristics of the sieved portions, suitable concentration or enzyme treatment is selected to obtain concentrated protein products that meet specific protein content requirements and / or functional characteristics.

[0014] Preferably, in step (1), the particle size of the large-particle oilseed meal is >500μm, the particle size of the medium-particle oilseed meal is 100-500μm, and the particle size of the small-particle oilseed meal is <100μm.

[0015] Preferably, in step (2), the inter-plate electric field strength of the air-electric field separation treatment is 0.5 to 25 kV / cm, and the air flow rate is 1 to 30 L / min.

[0016] Preferably, both the first and second concentration methods are countercurrent gradient alcohol extraction.

[0017] Preferably, the ethanol concentration in the countercurrent gradient alcohol extraction is increased from 10-65% to 50-80%, and the material-to-liquid ratio is 1:(8-20).

[0018] Preferably, the solid-liquid ratio of the enzyme treatment is 1:(4-20), and the mass ratio of the enzyme to medium-particle oilseed meal is 1:(50-500).

[0019] Preferably, the temperature of the alcohol enzyme treatment is 20-60°C, and the treatment time is 0.5-5 hours.

[0020] Preferably, the enzyme used in the alcohol enzyme treatment includes glycosidase or protease.

[0021] Preferably, after the first concentration yields the concentrated protein, an enzyme modification step is also included to obtain a functional concentrated protein.

[0022] Preferably, the first or second concentration is followed by a post-processing step; the post-processing includes modification, ultrasonication, sterilization, and drying.

[0023] Preferably, the modification is performed by adjusting the pH to neutral; and / or,

[0024] The power density of the ultrasound is 0.5–1 W / cm². 2 The time is 5 to 30 minutes.

[0025] Compared with the prior art, the present invention has the following advantages and technical effects:

[0026] This invention utilizes pulverization, sieving, electrostatic adsorption, air fractionation, and enzymatic hydrolysis to apply differentiated pretreatment schemes to different fractionated components, thereby simultaneously achieving the goals of increasing the concentrated protein content and regulating its functional properties.

[0027] The method provided by this invention makes full use of high-temperature meal resources, and can share some equipment with the low-temperature meal alcohol concentration method for protein, and also has the advantage of customizable product quality. Attached Figure Description

[0028] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0029] Figure 1 This is a process flow diagram of the method for preparing concentrated protein from highly denatured oilseed meal in Example 5 of the present invention;

[0030] Figure 2 A schematic diagram of the equipment used in the method for preparing concentrated protein from highly denatured oilseed meal provided by the present invention;

[0031] Among them, 1-crusher, 2-screening machine, 3-lifting platform, 4-gas cylinder, 5-air-electric field separation device, 6-conveyor, 7-sample inlet, 8-leaching device, 9-solution storage tank, 10-wet meal conveyor, 11-high temperature instantaneous sterilizer, 12-homogenizer, 13-spray drying tower;

[0032] Figure 3 This is a front view of the air-electric field separation device;

[0033] Figure 4 This is an isometric view of the air-electric field separation device from northeastern China.

[0034] Among them, 1-gas cylinder, 2-gas-solid mixing chamber, 3-spiral tube, 4-separation chamber, 5-electrode plate, 6-collection bag, 7-bag dust collector. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0037] This invention provides a method for preparing concentrated protein from highly denatured oilseed meal, comprising the following steps:

[0038] (1) The highly denatured oilseed meal was crushed and screened to obtain large-particle oilseed meal, medium-particle oilseed meal and small-particle oilseed meal respectively.

[0039] (2) The large-particle oilseed meal obtained in step (1) is subjected to air-electric field separation treatment to obtain high-protein component and high-fiber component respectively;

[0040] The high-protein component obtained in step (2) and the small-particle oilseed meal obtained in step (1) are respectively subjected to a first concentration to obtain concentrated protein;

[0041] The medium-particle oilseed meal obtained in step (1) is subjected to alcohol enzyme treatment and second concentration to obtain functional concentrated protein.

[0042] In a preferred embodiment, in step (1), the crude protein content of the highly denatured oilseed meal is 41-44%.

[0043] In a preferred embodiment, in step (1), the pulverization method is grinding; the grinding speed is 20000-35000 rpm, and the time is 30-60 s. This invention pulverizes highly denatured oilseed meal through short-time high-speed grinding, which achieves the purpose of pulverizing highly denatured oilseed meal while avoiding excessive grinding time that would aggravate protein denaturation.

[0044] In a preferred embodiment, in step (1), the large-particle oilseed meal has a particle size >500μm, the medium-particle oilseed meal has a particle size of 100-500μm, and the small-particle oilseed meal has a particle size <100μm. This invention utilizes the microstructural characteristics of oilseed meal to grade highly denatured oilseed meal into oilseed meals of different particle sizes and protein contents through appropriate crushing and sieving. The grading method using crushing and sieving has the advantages of low power consumption and environmental friendliness.

[0045] In a preferred embodiment, in step (2), the interplate electric field strength of the air-electric field separation treatment is 0.5–25 kV / cm, the air velocity is 1–30 L / min, and the treatment time is determined according to the processing volume. This invention utilizes the distribution patterns and physical properties of proteins and structural carbohydrates in the microstructure of oilseed meal. By performing air-electric field separation treatment on large-particle oilseed meal, high-fiber components that are difficult to remove in traditional processes are separated, resulting in a high-protein component with NSI similar to that of low-denatured oilseed meal and high gelation properties. The interplate electric field strength and air velocity of the air-electric field separation treatment both affect the separation effect of the high-protein and high-fiber components. The high-protein component carries more positive charge through friction and moves towards the negative electrode in the electric field; the high-fiber component is closer to the positive electrode region. By controlling the charge-to-mass ratio, the separation efficiency can be improved. Excessive air velocity will carry away some high-protein components, reducing the protein recovery rate, while insufficient air velocity will fail to effectively separate the high-protein and high-fiber components, affecting the separation effect.

[0046] In a preferred embodiment, both the first and second concentrations are performed using countercurrent gradient alcohol extraction. This invention increases the protein content in the product through countercurrent gradient alcohol extraction.

[0047] In a preferred embodiment, the high-protein component and the small-particle oilseed meal can be concentrated separately or mixed and then concentrated.

[0048] In a preferred embodiment, the ethanol concentration is increased from 10-60% to 50-75% during the first concentration process, the material-to-liquid ratio is 1:(8-20), and the temperature is 50-60°C. In a preferred embodiment of the present invention, countercurrent gradient ethanol extraction can be produced using industry-standard equipment, including an ethanol recovery device, a material conveying device, and a drying device. The present invention achieves concentration by employing a countercurrent gradient ethanol extraction method; using the aforementioned ethanol concentration is beneficial for obtaining high-purity concentrated protein.

[0049] In a preferred embodiment, the solid-liquid ratio of the enzyme treatment is 1:(4-20), and the mass ratio of enzyme to medium-particle oilseed meal is 1:(50-500); the alcohol used in the enzyme treatment is ethanol; and the volume concentration of the ethanol is 20-65%. This invention, by treating medium-particle oilseed meal with an enzyme, can obtain a high water-holding capacity concentrated protein with specific functional properties, and can also improve the protein content and digestibility of the product. The concentrated protein product obtained after enzyme hydrolysis and concentration at different ethanol concentrations has different protein content, and the concentrated protein product obtained after hydrolysis and concentration with different enzymes at the same ethanol concentration also has different protein content.

[0050] In a preferred embodiment, the alcohol enzyme treatment is performed at a temperature of 20–60°C for 0.5–5 hours, and the pH is adjusted to a suitable range for the enzyme. Performing the alcohol enzyme treatment at the above temperatures ensures the enzyme's activity.

[0051] In a preferred embodiment, the enzyme used in the alcohol enzyme treatment includes glycosidase or protease.

[0052] In a preferred embodiment, during the second concentration process, the ethanol concentration is increased from 10-65% to 50-80%, the material-to-liquid ratio is 1:(8-20), and the temperature is 50-60°C.

[0053] In a preferred embodiment, after the first concentration yields the concentrated protein, an enzyme modification step is further included to obtain a functional concentrated protein.

[0054] In a preferred embodiment, the first or second concentration is followed by a post-processing step; the post-processing includes modification, sonication, sterilization, and drying. The concentrated protein obtained after concentration has poor solubility, which can be improved through modification.

[0055] In a preferred embodiment, the modification is performed by adjusting the pH to neutral.

[0056] In a preferred embodiment, the power density of the ultrasound is 0.5–1 W / cm². 2 The time is 5 to 30 minutes.

[0057] In a preferred embodiment, a schematic diagram of the equipment used in the method for preparing concentrated protein from highly denatured oilseed meal provided by the present invention is shown below. Figure 2 The equipment includes a crusher, a sieve, a lifting platform, gas cylinders, an air-electric field separation device, a conveyor, a sample inlet, an extractor, a solution storage tank, a wet meal conveyor, an ultrasonic machine, and a spray drying tower.

[0058] In a preferred embodiment, the front view of the air-electric field separation device is shown below. Figure 3 Northeast isometric view Figure 4The device includes a gas cylinder, a gas-solid mixing chamber, a spiral tube, a separation chamber, electrode plates, a collection bag, and a bag filter. The separation chamber includes two electrode plates, one of which is grounded (negative electrode) and the other is connected to the positive electrode. The negative electrode collects high protein components, and the positive electrode collects high fiber components. The spiral tube is made of materials such as nylon, aluminum, or stainless steel.

[0059] The air-electric field separation process in this invention involves separating large, difficult-to-crush particles with low protein content from oilseed meal after sieving. These large particles rub against each other within the spiral separation tube and also come into contact with the chamber walls. Protein-rich particles tend to lose electrons and become positively charged, while carbohydrate-rich particles (such as fiber) tend to gain electrons and become negatively charged. After being carried out by the airflow, under the combined influence of the electric field and gravity, particles with different compositions exhibit differentiated distribution patterns: heavier particles are closer to the electrodes, while lighter, high-protein-purity particles fall to the far end. Four or more collection bags are placed between the two electrodes to divide the separation area, which can be customized according to the type and properties of the oilseed meal. High-purity protein particles tend to be distributed at the far end of the separation field (away from the spiral tube opening); components with high fiber content tend to be closer to the near end of the separation field (closer to the spiral tube opening). The separated material can be separated again, or a magnetic field module can be added between the electrodes to further improve the separation degree of protein and carbohydrates. Airflow velocity, electric field strength, magnetic field strength, number of segmented regions, and material particle size can be adjusted according to separation efficiency and objectives.

[0060] In this embodiment of the invention, room temperature refers to "25±2℃".

[0061] Unless otherwise specified, all raw materials used in the embodiments of this invention were purchased through commercial channels.

[0062] Example 1

[0063] (1) The high-temperature oilseed meal with a crude protein content of 41% was ground at 30,000 rpm for 30 seconds using a pulverizer and sieved to obtain large-particle oilseed meal M1 with a particle size of >500μm, medium-particle oilseed meal M2 with a particle size of 100-500μm and small-particle oilseed meal M3 with a particle size of <100μm.

[0064] (2) The large-particle oilseed meal M1 obtained in step (1) was subjected to air-electric field separation treatment under the conditions of an inter-plate electric field strength of 10kV / cm and an air flow rate of 15L / min. After 4min, high-protein component ME1 and high-fiber component ME2 were obtained respectively.

[0065] The protein content of ME1, the high-protein component obtained in Example 1, was determined to be 70% using the Kjeldahl method, which meets commercial requirements, and the nitrogen solubility index was higher than 65%.

[0066] Weigh 5.0g ME1 and dissolve it in 20mL of water. Stir at room temperature for 2 hours to disperse it evenly. Heat in a 90℃ water bath for 30 minutes, then cool rapidly with running water. Place in a 4℃ refrigerator overnight. Measure the gel strength using a texture analyzer with a trigger force of 5g. The measured gel strength is >400g.

[0067] Example 2

[0068] (1) The high-temperature oilseed meal with a crude protein content of 41% was ground at 30,000 rpm for 0.5 min using a pulverizer and sieved to obtain large-particle oilseed meal M1 with a particle size of >500 μm, medium-particle oilseed meal M2 with a particle size of 100-500 μm and small-particle oilseed meal M3 with a particle size of <100 μm.

[0069] (2) At 60℃, the small-particle oilseed meal M3 obtained in step (1) was subjected to countercurrent gradient alcohol extraction, with the ethanol concentration increased from 30% to 75% and the solid-liquid ratio at 1:20, to obtain concentrated protein PC3. The pH of PC3 was adjusted to neutral, and then the mixture was processed at a power density of 1W / cm³. 2 Under certain conditions, the protein is ultrasonically treated for 15 minutes, sterilized, dried, pulverized, and packaged to obtain the final product, concentrated protein.

[0070] The protein content of PC3 was determined to be >65% and the nitrogen solubility index (NSI) was >40% using the Kjeldahl method.

[0071] Example 3

[0072] (1) The high-temperature oilseed meal with a crude protein content of 41% was ground at 30,000 rpm for 0.5 min using a pulverizer and sieved to obtain large-particle oilseed meal M1 with a particle size of >500 μm, medium-particle oilseed meal M2 with a particle size of 100-500 μm and small-particle oilseed meal M3 with a particle size of <100 μm.

[0073] (2) The medium-particle oilseed meal M2 obtained in step (1) was mixed with glycosidase and ethanol for alcohol enzyme treatment. The mass ratio of glycosidase to medium-particle oilseed meal M2 was 1:100, the volume concentration of ethanol was 65%, the solid-liquid ratio was 1:5, the pH was adjusted to 5.0±0.1, and the temperature was controlled at 50℃. After 1 hour, EM2 was obtained. At 60℃, the above EM2 was subjected to countercurrent gradient alcohol extraction, the ethanol concentration was increased from 20% to 55%, and the solid-liquid ratio was 1:20 to obtain functional concentrated protein PCEM2. The pH of PCEM2 was adjusted to neutral, and then the power density was 1W / cm³. 2 Under certain conditions, the protein is ultrasonically treated for 15 minutes, sterilized, dried, pulverized, and packaged to obtain the finished functional concentrated protein.

[0074] The protein content of PCEM2 was >70% as determined by the Kjeldahl method.

[0075] Example 4

[0076] (1) The high-temperature oilseed meal with a crude protein content of 41% was ground at 30,000 rpm for 30 seconds using a pulverizer and sieved to obtain large-particle oilseed meal M1 with a particle size of >500μm, medium-particle oilseed meal M2 with a particle size of 100-500μm and small-particle oilseed meal M3 with a particle size of <100μm.

[0077] (2) The medium-particle oilseed meal M2 obtained in step (1) is mixed with protease and ethanol for alcohol enzyme treatment. The mass ratio of protease to medium-particle oilseed meal M2 is 1:50, the volume concentration of ethanol is 65%, the solid-liquid ratio is 1:5, the pH is adjusted to 8.0±0.1, the temperature is controlled at 50℃, and after 1 hour, EM2 is obtained. At 60℃, the above EM2 is subjected to countercurrent gradient alcohol extraction, the ethanol concentration is increased from 65% to 80%, the material-liquid ratio is 1:20, and the functional concentrated protein PCEM2 is obtained. PCEM2 is sterilized, dried, pulverized and packaged to obtain the finished functional concentrated protein.

[0078] The protein content of PCEM2 was >65% and NSI >25% as determined by the Kjeldahl method; the gel strength of PCEM2 was >500g as determined by the method in Example 1.

[0079] Example 5

[0080] A method for preparing concentrated protein from highly denatured oilseed meal, the process flow is as follows: Figure 1 A schematic diagram of the equipment used is shown below. Figure 2 The specific steps are as follows:

[0081] (1) The high-temperature oilseed meal with a crude protein content of 41% was ground at 30,000 rpm for 30 seconds using a pulverizer and sieved to obtain large-particle oilseed meal M1 with a particle size of >500μm, medium-particle oilseed meal M2 with a particle size of 100-500μm and small-particle oilseed meal M3 with a particle size of <100μm.

[0082] (2) The large-particle oilseed meal M1 obtained in step (1) was subjected to air-electric field separation treatment under the conditions of an inter-plate electric field strength of 10kV / cm and an air flow rate of 15L / min to obtain high-protein component ME1 and high-fiber component ME2 respectively.

[0083] (3) At 60°C, the high protein component ME1 obtained in step (2) and the small-particle oilseed meal M3 obtained in step (1) were subjected to countercurrent gradient alcohol extraction. The ethanol concentration was increased from 60% to 75% and the material-liquid ratio was 1:10, respectively, to obtain concentrated protein PCME1 and PC3.

[0084] (4) The medium-particle oilseed meal M2 obtained in step (1) was mixed with protease and ethanol for alcohol enzyme treatment. The mass ratio of protease to medium-particle oilseed meal M2 was 1:50, the volume concentration of ethanol was 65%, the solid-liquid ratio was 1:10, the pH was adjusted to 9.0±0.1, the temperature was controlled at 60℃, and after 1 h, EM2 was obtained. At 60℃, the above EM2 was subjected to countercurrent gradient alcohol extraction, the ethanol concentration was increased from 60% to 75%, the material-liquid ratio was 1:20, and the functional concentrated protein PCEM2 was obtained.

[0085] (5) Adjust the pH of the concentrated proteins PCME1 and PC3 obtained in step (3) and the functional concentrated protein PCEM2 obtained in step (4) to neutral, and then heat them at a power density of 1 W / cm³. 2 Under the conditions of ultrasonic treatment for 15 minutes, sterilization, drying, pulverization and packaging were carried out to obtain finished products PC3, PCME1 and PCEM2 respectively.

[0086] The protein content of finished product PC3 was >65% and NSI >25% as determined by the Kjeldahl method; the protein content of finished product PCME1 was >70% and NSI >25%; and the protein content of finished product PCEM2 was >65% and NSI >25%.

[0087] The above are merely preferred embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A method for preparing concentrated protein from highly denatured oilseed meal, characterized in that, Includes the following steps: (1) The highly denatured oilseed meal is crushed and sieved to obtain large-particle oilseed meal, medium-particle oilseed meal and small-particle oilseed meal respectively; the particle size of the large-particle oilseed meal is >500μm, the particle size of the medium-particle oilseed meal is 100~500μm, and the particle size of the small-particle oilseed meal is <100μm. (2) The large-particle oilseed meal obtained in step (1) is subjected to air-electric field separation treatment to obtain high-protein component and high-fiber component respectively; the interplate voltage of the air-electric field separation treatment is 0.5~25kV / cm, and the air flow rate is 1~30L / min; The high-protein component obtained in step (2) and the small-particle oilseed meal obtained in step (1) are respectively subjected to a first concentration to obtain concentrated protein; The medium-particle oilseed meal obtained in step (1) is subjected to alcohol enzyme treatment and second concentration to obtain functional concentrated protein; the enzyme used in the alcohol enzyme treatment is glycosidase or protease. The specific operation of the alcohol enzyme treatment is as follows: medium-sized oilseed meal is mixed with enzyme and ethanol for alcohol enzyme treatment. The volume concentration of ethanol is 65%, the pH is adjusted to the suitable range of the enzyme, and after 0.5 to 5 hours, functional concentrated protein is obtained. The highly denatured oilseed meal is a high-temperature meal with a crude protein content of 41%-44%; Both the first and second concentrations are performed using countercurrent gradient alcohol extraction. The ethanol concentration of the first concentrated countercurrent gradient alcohol extraction includes increasing from 30% to 75%, and the ethanol concentration of the second concentrated countercurrent gradient alcohol extraction includes increasing from 20% to 55%, from 60% to 75%, or from 65% to 80%, with a material-to-liquid ratio of 1:(8-20).

2. The method for preparing concentrated protein from highly denatured oilseed meal according to claim 1, characterized in that, The solid-liquid ratio of the enzyme treatment is 1:(4-20), and the mass ratio of enzyme to medium-particle oilseed meal is 1:(50-500).

3. The method for preparing concentrated protein from highly denatured oilseed meal according to claim 1, characterized in that, The alcohol enzyme treatment is performed at a temperature of 20–60°C for a duration of 0.5–5 hours.

4. The method for preparing concentrated protein from highly denatured oilseed meal according to claim 1, characterized in that, The first concentration process, after obtaining the concentrated protein, also includes an enzyme modification step to obtain a functional concentrated protein.

5. The method for preparing concentrated protein from highly denatured oilseed meal according to claim 1, characterized in that, The first or second concentration is followed by a post-processing step; the post-processing includes modification, ultrasonication, sterilization, and drying. The modification is performed by adjusting the pH to neutral; and / or, The power density of the ultrasound is 0.5–1 W / cm². 2 The time is 5 to 30 minutes.

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