Liquid chromatography analyzer with multi-channel chromatography column

Abstract

The present invention discloses a liquid chromatograph with a multi-channel chromatographic column, which relates to the field of chromatographic analysis technology, including an infusion system, a sample injection system, a separation system and a detection system. The separation system is used to separate the components of a sample, and the separation system includes a chromatographic column; a main channel and a secondary channel for liquid circulation are provided in the chromatographic column; the chromatographic column is also provided with an adjustment component and a switching component; through the adjustment of the adjustment component, the liquid flows out from the main channel or the secondary channel; the switching component is used to switch the flow direction of the liquid and perform backwash cleaning on the inside of the chromatographic column; the detection system is used to detect the liquid and sample component data flowing out of the chromatographic column, and send the detection data to a computer and a printing system, and the printing system prints a chromatogram. By arranging the above-mentioned components, the present invention effectively improves the distribution uniformity of the liquid phase, meets the detection requirements applicable to different flow rates, increases the function of backwash cleaning, and improves the operational convenience of the chromatograph.

Description

Technical Field

[0001] The present invention relates to the technical field of chromatography analysis, in particular to a liquid chromatography analyzer with a multi-channel chromatography column. Background Art

[0002] UPLC, a core technology for analyzing the chemical components of traditional Chinese medicine (TCM), has a separation efficiency directly related to chromatographic column performance. However, TCM chemical components are characterized by high complexity, a wide molecular weight distribution, and some components exhibiting high viscosity. For example, polysaccharides, colloids, and some alkaloids require mobile phases with significantly higher viscosities than conventional analytical samples.

[0003] When performing chromatographic analysis on liquid phases of traditional Chinese medicine, small solid impurities are likely to remain inside, causing clogging of the chromatographic column. Traditional UPLC columns generally suffer from low backflush cleaning efficiency when dealing with liquid phases of traditional Chinese medicine.

[0004] Conventional chromatographic columns use fillers with fixed pore structures and homogeneous filling technology. When analyzing high-viscosity samples, the column pressure is easily increased due to the increased mass transfer resistance of the mobile phase, which requires disassembly of the chromatographic column and replacement of the flow control plate with the corresponding passable area according to the viscosity of the sample to be tested. This operation is very troublesome. In addition, the traditional backflush operation requires disassembly of the chromatographic column and replacement of the inlet and outlet. Multiple disassembly can easily cause the end of the chromatographic column to loosen, resulting in leakage due to high pressure during use, affecting the accuracy of the experimental data.

[0005] Traditional chromatographic columns have the above problems during use, and the flow rate of the chromatographic column cannot be adjusted according to experimental requirements, resulting in uneven flow of the liquid phase in the filler at low flow rates, resulting in poor peak symmetry of the chromatographic column and affecting the experimental results.

[0006] Therefore, it is necessary to invent a liquid chromatograph with a multi-channel chromatographic column to solve the above problems. Summary of the Invention

[0007] The object of the present invention is to provide a liquid chromatography analyzer with a multi-channel chromatography column to solve the problems raised in the above background technology.

[0008] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a liquid chromatography analyzer with a multi-channel chromatographic column, comprising: an infusion system for conveying mobile phase liquid; an injection system for inputting samples and mixing them with the mobile phase liquid; a separation system for separating the components of the sample, the separation system comprising a chromatographic column; a main channel and a secondary channel for liquid circulation are provided in the chromatographic column; the chromatographic column is also provided with an adjustment component and a switching component; the sample input by the injection system can flow out from the main channel or the secondary channel through the adjustment of the adjustment component; when the liquid flows out from the main channel, the flow rate of the sample in the filler can be made uniform; when the liquid flows out from the secondary channel, it facilitates the circulation of viscous liquid; a switching component is arranged in the chromatographic column and cooperates with the adjustment component to switch the liquid flow direction and backflush the inside of the chromatographic column; a detection system is used to detect the component data of the sample flowing out of the chromatographic column, send the detection data to a computer, and output a chromatogram.

[0009] Preferably, the main flow channel and the secondary flow channel are both opened on one side of the chromatographic column close to the output end; the main flow channels and the secondary flow channels are the same in number and are staggered with each other along the axial direction; the main flow channels and the secondary flow channels both extend radially and expand to both sides; the area occupied by the main flow channel is smaller than the area occupied by the secondary flow channel.

[0010] Preferably, the adjustment component includes: a switching tube, which can selectively communicate with the main channel / secondary channel; a switching sleeve, which is rotatably sleeved on the outside of the chromatographic column and fixedly connected to the switching tube, for adjusting the communication state between the switching tube and the main channel / secondary channel.

[0011] Preferably, the chromatographic column further comprises: a column tube, one end of which is connected to a liquid inlet tube, the other end of which is connected to the liquid inlet end of the chromatographic column, the other end of the column tube is connected to a liquid outlet tube, the other end of which is connected to the liquid outlet end of the chromatographic column; a filter part, which is placed in the column tube and fixedly connected to both ends of the column tube, and is used to filter impurities in the liquid and sample.

[0012] Preferably, the switching assembly includes: an upper liquid pipe and a lower liquid pipe, both of which are placed outside the column tube; a first valve body, which is used to control the on-off of the liquid inlet pipe; a second valve body, which is used to control the on-off of the upper liquid pipe; a third valve body, which is used to control the on-off of the liquid outlet pipe; and a fourth valve body, which is used to control the on-off of the lower liquid pipe.

[0013] Preferably, when the first valve body is connected to the third valve body, the sample and the liquid pass through the liquid inlet tube, the column tube, and the liquid outlet tube in sequence to perform sample separation; when the second valve body is connected to the fourth valve body, the liquid passes through the liquid inlet tube, the upper liquid tube, the column tube, the lower liquid tube, and the liquid outlet tube in sequence to perform backflushing cleaning of the chromatographic column.

[0014] Preferably, the groove depths in the central regions of the main flow channel and the secondary flow channel are greater than the groove depths in the edge regions thereof, thereby suppressing the central flow velocity and reducing the flow resistance.

[0015] Preferably, the end of the main flow channel extends to the inner side of the column tube, and the end of the secondary flow channel is close to the inner side of the column tube.

[0016] Technical effects and advantages of the present invention:

[0017] 1. The present invention effectively achieves the purpose of improving the uniformity of liquid phase distribution by providing a column tube, a filter part, a main flow channel, a secondary flow channel and an adjustment component. The filter part on one side of the water inlet pipe is a multi-layer structure, and the density increases from top to bottom, thereby improving the uniformity of liquid distribution in the column tube and filtering impurities in the liquid phase. In addition, through the cooperation of the adjustment component and the main flow channel / secondary flow channel, it is possible to control the liquid flow rate in the column tube while still maintaining the uniform distribution of the sample, thereby improving the peak symmetry of the chromatographic column and achieving the purpose of being applicable to multiple flow rates.

[0018] 2. The present invention achieves the purpose of being suitable for viscous liquid phases by setting up main channels and secondary channels. The depths of the main channels and secondary channels from the center to the edge area gradually change, which can suppress excessive center flow velocity and reduce flow resistance. The liquid enters the liquid inlet through the secondary channel. The secondary channel occupies a larger area than the main channel, and a high-viscosity liquid phase can pass through the filler, reducing the risk of blockage. It can also be applied to liquid phase flow rates between high flow and low flow.

[0019] 3. The present invention effectively achieves the backflushing effect by setting a switching component. When in use, by controlling the conduction state of the first valve body, the second valve body, the third valve body and the fourth valve body, the liquid is allowed to flow back to the water inlet end through the water outlet end of the column tube, and the internal filler, main flow channel, secondary flow channel and filter part are backflushed and cleaned.

[0020] 4. The present invention improves the safety of the chromatographic column by providing an outer sleeve. The outer sleeve is arranged on the outside of the column tube to protect the column tube and prevent the packing from moving due to collision. A gap is provided between the outer sleeve and the column tube so that it can act as a buffer. In addition, by providing an index ring and a scale, the working status of the chromatographic column can be easily judged. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Flowchart of the present invention.

[0022] Figure 2 Schematic diagram of the overall structure of the chromatographic column in the present invention.

[0023] Figure 3 It is a cross-sectional view of the chromatographic column in the present invention.

[0024] Figure 4 Schematic diagram of the explosion at the top of the chromatographic column in the present invention.

[0025] Figure 5 Schematic diagram of the explosion at the bottom of the chromatographic column in the present invention.

[0026] Figure 6 It is a structural schematic diagram of the adjustment component in the present invention.

[0027] Figure 7 Schematic diagram of the distribution of the main flow channel and the secondary flow channel in the present invention.

[0028] Figure 8 It is a cross-sectional view of the adjustment component in the present invention.

[0029] Figure 9 Schematic diagram of the positions of the lower liquid pipe and the upper liquid pipe in the present invention.

[0030] Figure 10 Schematic diagram of liquid flow during operation and backflushing of the chromatographic column in the present invention.

[0031] In the figure: 1. Infusion system; 2. Sample injection system; 3. Separation system; 4. Chromatographic column; 41. Main flow channel; 42. Secondary flow channel; 43. Adjustment assembly; 431. Switching tube; 432. Switching sleeve; 433. Liquid inlet notch; 44. Switching assembly; 441. Upper liquid pipe; 442. Lower liquid pipe; 443. First valve body; 444. Second valve body; 445. Third valve body; 446. Fourth valve body; 5. Detection system; 6. Computer; 7. Column tube; 8. Liquid inlet pipe; 9. Liquid outlet pipe; 10. Filter unit; 11. Connector; 12. Connecting sleeve; 13. Conical mouth. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] In embodiment 1, the present invention provides Figures 1 to 8The liquid chromatography analyzer with a multi-channel chromatographic column shown includes an infusion system 1 for conveying liquid, i.e., mobile phase; an injection system 2 for inputting a test sample and mixing it with the mobile phase; a separation system 3 for separating the components of the sample; a detection system 5 for detecting the liquid and sample component data flowing out of the chromatographic column 4, and sending the detection data to a computer 6 and a printing system, which prints a chromatogram. The infusion system 1, the injection system 2, and the detection system 5 are all prior art, and the specific structures are not described again. The computer 6 mainly performs analysis and processing of the detection data, and its analysis and processing method is also prior art.

[0034] The separation system 3 includes a chromatographic column 4 and may also include other components assisting separation, such as a thermostat for controlling the temperature of the chromatographic column 4 .

[0035] The chromatographic column 4 is provided with a main channel 41 and a secondary channel 42 for liquid circulation. The chromatographic column 4 is filled with filler, that is, the stationary phase. The main channel 41 and the secondary channel 42 are both placed at the output end of the filler in the chromatographic column 4; the chromatographic column 4 is also provided with an adjustment component 43 and a switching component 44, and the flow direction of the liquid and the sample is changed by the adjustment component 43.

[0036] The sample input by the injection system 2 can flow out from the main channel 41 or the secondary channel 42 through the adjustment of the adjustment component 43. It should be pointed out that the sample follows the flow of the liquid input by the infusion system 1; when the liquid flows out from the main channel 41, the flow velocity of the sample in the filler is uniform. In the process of the liquid flowing out through the main channel 41, the sample encounters resistance at the center of the seasoning, which reduces the flow velocity at the center position and increases the flow velocity at the edge position, so that the sample flows evenly in the filler and improves the symmetry of the peak shape.

[0037] When the liquid flows out of the secondary flow channel 42 , it facilitates the circulation of the viscous liquid. The secondary flow channel 42 is more diffusely distributed and occupies a larger area, so that the viscous liquid can also pass through the filler evenly and stably, thereby improving the applicability of the chromatographic column 4 .

[0038] Specifically, the main channel 41 and the secondary channel 42 are both opened on one side of the chromatographic column 4 close to the output end, and the liquid and the sample first pass through the filler and then enter the main channel 41 or the secondary channel 42; the main channels 41 and the secondary channels 42 are the same in number and are staggered with each other along the axial direction. The groove depths in the central areas of the main channel 41 and the secondary channel 42 are greater than the groove depths in their edge areas, which suppresses the central flow velocity and reduces the flow resistance. During use, the liquid flow velocity in the middle of the filler is limited, so that the sample is more evenly distributed at the same height position in the filler.

[0039] Both the main channel 41 and the secondary channel 42 extend radially and expand to both sides. The main channel 41 expands to both sides for a longer distance but has a smaller expansion area, while the secondary channel 42 expands to both sides for a shorter distance but has a larger expansion area. The area occupied by the main channel 41 is smaller than that occupied by the secondary channel 42. The setting of the secondary channel 42 makes it easier for viscous liquids to pass through. Since the secondary channel 42 occupies a larger area, when non-viscous liquids pass through the secondary channel 42, the liquid passes through faster, which is suitable for high flow situations, so that the chromatographic column 4 can meet the requirements of various flow requirements.

[0040] More specifically, the adjustment component 43 includes: a switching tube 431, which can selectively communicate with the main channel 41 / secondary channel 42, and the switching tube 431 is placed at the center of the axial distribution of the main channel 41 and the secondary channel 42. A liquid inlet notch 433 is opened at the upper end of the switching tube 431, and the number of the liquid inlet notch 433 is the same as the main channel 41 / secondary channel 42. When in use, by rotating the switching tube 431 to an appropriate angle, the liquid inlet notch 433 can be adjusted to correspond to and be linked with the main channel 41 / secondary channel 42; a switching sleeve 432, which is rotatably sleeved on the outside of the chromatographic column 4 and fixedly connected to the switching tube 431, is used to adjust the communication state of the switching tube 431 and the main channel 41 / secondary channel 42. The switching sleeve 432 has the function of protecting the column tube 7 to prevent the packing from moving due to collision. During use, the switching sleeve 432 is rotated to rotate the switching sleeve 432 relative to the column tube 7, thereby adjusting the relative angle of the switching tube 431 relative to the main channel 41 / secondary channel 42.

[0041] It should be pointed out that in actual use, in addition to setting the main channel 41 and the secondary channel 42, a third channel, a fourth channel and the like can be set as needed to guide the flow of the liquid, so as to achieve the purpose of being applicable to liquids of more properties, and the distribution area and shape of the corresponding channel can be set according to the properties of the targeted liquid to increase the applicable liquid. At the same time, the liquid inlet notch 433 opened at the upper end of the switching tube 431 is also modified synchronously so that when the switching tube 431 is rotated, only a single channel can be opened.

[0042] Specifically, the chromatographic column 4 also includes: a column tube 7, one end of which is connected to a liquid inlet tube 8, the other end of the liquid inlet tube 8 is connected to the liquid inlet end of the chromatographic column 4, the other end of the column tube 7 is connected to a liquid outlet tube 9, the other end of the liquid outlet tube 9 is connected to the liquid outlet end of the chromatographic column 4, and the filler is placed in the column tube 7; a filter part 10, which is placed in the column tube 7 and fixedly connected to both ends of the column tube 7, and is used to filter impurities in the liquid and the sample. The filter part 10 close to the side of the liquid inlet tube 8 is a multi-layer structure, and the density increases from top to bottom, thereby improving the uniformity of liquid distribution and filtering impurities in the liquid. The filter part 10 close to the side of the liquid outlet tube 9 is only used to fix the filler.

[0043] It should be noted that the main flow channel 41 and the secondary flow channel 42 are provided between the liquid outlet pipe 9 and the corresponding filter portion 10 .

[0044] More specifically, the end of the main channel 41 extends to the inside of the column tube 7, and the end of the secondary channel 42 is close to the inside of the column tube 7. Therefore, when in use, it is effectively prevented that the liquid enters the gap between the filler and the column tube 7 through the circumference of the filler, causing part of the liquid to enter the inoperative main channel 41 / secondary channel 42, so that some components of the sample cannot be discharged from the chromatographic column 4 in time, or cannot be discharged from the chromatographic column 4, affecting the accurate value of the experimental data.

[0045] Specifically, the chromatographic column 4 also includes a connector 11, which is rotatably connected to the two ends of the switching sleeve 432, and is used to connect the pipes for liquid inlet and outlet of the chromatographic column 4. The connector 11 can adopt the shape of a hexagonal bolt to facilitate the connection of the pipe, but the specific shape is not specifically limited in this application; the column tube 7 is fixedly connected to the connector 11; the inner side of each connector 11 is fixedly connected to a connecting sleeve 12, and a tapered mouth 13 is provided in the middle of the connecting sleeve 12, and the end of the tapered mouth 13 is connected to the corresponding liquid inlet pipe 8 and liquid outlet pipe 9. The setting of the tapered mouth 13 can make the pipe and the connecting sleeve 12 more tightly connected, so that it can cope with higher pressure. It should be noted that the end of the connector 11 is provided with a thread to facilitate the connection of external pipes, and the thread is not shown in the figure.

[0046] More specifically, a tooth-like structure is provided on the outer side of the switching sleeve 432, which can rotate the switching sleeve 432 through a driving component such as a stepper motor or a servo. The tooth-like structure, the stepper motor, and the servo are all existing technologies and will not be described in detail here. They are also not shown in the figure. The stepper motor or the servo is connected to the computer 6 through an electrical signal.

[0047] In summary, the output liquid and sample pipes of the infusion system 1 and the injection system 2 are connected to the connector 11, and the pipes are connected to the corresponding connecting sleeves 12, and the pipes of the input detection system 5 are connected to another connecting sleeve 12, and then the switching tube 431 is adjusted according to the experimental requirements to connect with the main channel 41 / secondary channel 42.

[0048] When the experiment uses a liquid with a low flow rate, the switching sleeve 432 is rotated to connect the switching tube 431 with the main channel 41, and then the separated sample enters the detection system 5; when the experiment uses a viscous liquid, the switching sleeve 432 is rotated to connect the switching tube 431 with the secondary flow channel 42, and the separated sample enters the detection system 5; when the experiment requires increasing the liquid flow rate, the liquid is still allowed to pass through the secondary flow channel 42, so that the non-viscous liquid can stably and evenly pass through the separation of the chromatographic column 4 and enter the detection system 5.

[0049] Example 2: Through the above examples, it was found in actual work that the chromatographic column is prone to clogging during operation, especially when using liquids or samples containing fine particles. The clogging of the chromatographic column will cause an abnormal increase in pressure, resulting in an overload alarm or shutdown of the pump that transports the liquid, and even damage to the flow path of the chromatographic system. It will also cause abnormal peak shape and decreased separation efficiency, resulting in inaccurate chromatographic data generated, affecting the test results. Therefore, to address the problem of chromatographic column clogging, a flushing fluid is generally used to backwash the chromatographic column.

[0050] Specifically, such as Figures 7 to 10 , and also includes a switching component 44, which is arranged in the chromatographic column 4 and cooperates with the adjustment component 43 to switch the liquid flow direction and backflush the inside of the chromatographic column 4. The switching component 44 cleans the chromatographic column 4 by changing the flow direction of the liquid in the chromatographic column 4. When backflushing the chromatographic column 4 in the traditional way, the chromatographic column 4 needs to be manually disassembled and reinstalled after changing the direction. Multiple installations can easily cause damage to the connecting parts at the end of the chromatographic column 4, causing leakage and damage to the chromatographic column 4.

[0051] More specifically, the switching assembly 44 includes: an upper liquid tube 441 and a lower liquid tube 442, both of which are placed on the outside of the column tube 7, and the upper liquid tube 441 and the lower liquid tube 442 are used to switch the direction of liquid flow; a first valve body 443, which is used to control the on-off of the liquid inlet tube 8, and controls whether the liquid and the sample can enter the column tube 7 through the liquid inlet tube 8; a second valve body 444, which is used to control the on-off of the upper liquid tube 441, and controls whether the liquid can enter the column tube 7 through the output end of the column tube 7; a third valve body 445, which is used to control the on-off of the liquid outlet tube 9, and controls whether the liquid and the sample can be discharged from the column tube 7 through the liquid outlet tube 9; a fourth valve body 446, which is used to control the on-off of the lower liquid tube 442, and controls whether the liquid can be discharged from the column tube 7 through the input end of the column tube 7.

[0052] It should be noted that the first valve body 443, the second valve body 444, the third valve body 445 and the fourth valve body 446 are all electrically controlled valves and are electrically connected to the computer 6. The computer 6 controls the opening and closing of the first valve body 443, the second valve body 444, the third valve body 445 and the fourth valve body 446.

[0053] Specifically, when the first valve body 443 and the third valve body 445 are connected, the second valve body 444 and the fourth valve body 446 are closed, and the sample and the liquid pass through the liquid inlet pipe 8, the column pipe 7, and the liquid outlet pipe 9 in sequence to perform sample separation. At this time, it is the first state, that is, the normal working state of the chromatographic column 4. Figure 10 Middle a state.

[0054] When the second valve body 444 and the fourth valve body 446 are connected, the first valve body 443 and the third valve body 445 are closed, and the liquid passes through the liquid inlet pipe 8, the upper liquid pipe 441, the column pipe 7, the lower liquid pipe 442, and the liquid outlet pipe 9 in sequence to perform backflushing cleaning of the chromatographic column 4. At this time, it is the second state, that is, the backflushing state of the chromatographic column 4. Figure 10 Middle b state.

[0055] It should be noted that when blockage occurs during operation of the main channel 41, the liquid inlet notch 433 can be made to correspond to the main channel 41 during backflushing, the main channel 41 can be backflushed separately, and the secondary channel 42 can be backflushed for a short time for maintenance; similarly, the secondary channel 42 can also be backflushed separately to improve the backflushing cleaning effect, shorten the backflushing cleaning time, and reduce the liquid waste caused by backflushing.

[0056] It should be pointed out that, through the dispersed arrangement of the main flow channel 41 / the secondary flow channel 42, during the backflushing process, the flushing liquid can enter the column tube 7 in a dispersed manner through the main flow channel 41 / the secondary flow channel 42, and evenly backflushed the filler inside it, thereby preventing the backflushing flushing liquid from entering the column tube 7 and failing to reach the edge of the filler, resulting in the filler being unable to be effectively backflushed and cleaned in the circumferential direction; due to the different distribution areas and positions of the main flow channel 41 / the secondary flow channel 42, the flushing pressure on the filler during backflushing is also different. The expansion area of ​​the main flow channel 41 is smaller, so that the flushing liquid can enter the column tube 7 with a relative pressure and backflushed the filler, while the secondary flow channel 42 is smaller. The expanded area of ​​channel 42 is larger, so that the flushing liquid can be distributed more evenly when entering the column tube 7, and the flushing area of ​​the packing is larger. In actual use, the main channel 41 / the secondary channel 42 can be alternately connected to the liquid inlet notch 433 according to the blockage situation, so as to achieve the purpose of backflushing the packing as a whole. Compared with the traditional backflushing method, the backflushing effect is better (because the traditional backflushing method generally enters the flushing liquid from the liquid outlet and flows in the opposite direction for flushing. The flushing area and path depend on the size of the liquid inlet. The liquid outlet is generally relatively small, and it is easy to leave a large area of ​​flushing dead corner, resulting in poor backflushing effect).

[0057] In summary, when the chromatographic column 4 is working normally, the first valve body 443 and the third valve body 445 are connected, and the second valve body 444 and the fourth valve body 446 are closed. At the same time, the liquid drives the sample through the liquid inlet tube 8, the column tube 7, the main channel 41 / the secondary channel 42, the switching tube 431 and the liquid outlet tube 9 in sequence, and then the separated sample enters the detection system 5.

[0058] When the chromatographic column 4 is blocked, the first valve body 443 and the third valve body 445 are closed, and the second valve body 444 and the fourth valve body 446 are connected, and then the cleaning liquid is injected into the chromatographic column 4 through the liquid inlet system. Then the liquid passes through the liquid inlet pipe 8, the upper liquid pipe 441, the column tube 7, the lower liquid pipe 442, and the liquid outlet pipe 9 in sequence to backflush the chromatographic column 4. At the same time, by rotating the switching sleeve 432, the liquid can pass through the main channel 41 and the secondary channel 42 respectively to backflush the filler and the filter part 10. When the main channel 41 or the secondary channel 42 is blocked, a single channel can be backflushed separately to shorten the backflush time. The main channel 41 / secondary channel 42 can also be alternately connected to the liquid inlet notch 433 according to the blockage situation, so as to achieve a comprehensive backflush cleaning of the filler as a whole and improve the cleaning effect of the chromatographic column.

[0059] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A liquid chromatograph having a multi-channel chromatographic column, characterized in that: include: Infusion system, used to deliver mobile phase liquid; Injection system, used to input sample and mix it with mobile phase liquid; A separation system for separating components of a sample, comprising a chromatographic column; the chromatographic column is provided with a main flow channel and a secondary flow channel for liquid circulation; the chromatographic column is also provided with a regulating component and a switching component; The main flow channel and the secondary flow channel are both opened on one side of the chromatographic column close to the output end; The main flow channels and the secondary flow channels are the same in number and are staggered with each other along the axial direction; The main flow channel and the secondary flow channel both extend radially and expand toward both sides; The area occupied by the main flow channel is smaller than that occupied by the secondary flow channel; The sample input by the injection system can flow out from the main flow channel or the secondary flow channel through the adjustment of the adjustment component; When the liquid flows out of the main channel, the sample can flow evenly in the packing; When the liquid flows out from the secondary flow channel, it facilitates the flow of viscous liquid; The regulating assembly includes: a switching tube, which can selectively communicate with the main channel / auxiliary channel. The switching tube is placed at the center of the axial distribution of the main channel and the auxiliary channel. The upper end of the switching tube is provided with liquid inlet notches, the number of which is the same as the main channel / auxiliary channel. When in use, by rotating the switching tube to an appropriate angle, the liquid inlet notches can be adjusted to correspond to and communicate with the main channel / auxiliary channel; The switching sleeve is rotatably mounted on the outside of the chromatographic column and is fixedly connected to the switching tube to adjust the connection between the switching tube and the main flow channel / secondary flow channel; A switching component is provided in the chromatographic column and cooperates with the regulating component to switch the direction of liquid flow and perform backflushing cleaning on the inside of the chromatographic column; The groove depths in the central areas of the main flow channel and the secondary flow channel are greater than the groove depths in the edge areas, thereby suppressing the central flow velocity and reducing the flow resistance; The detection system is used to detect the component data of the sample flowing out of the chromatographic column, send the detection data to the computer, and output the chromatogram.

2. The liquid chromatograph with a multi-channel chromatographic column according to claim 1, characterized in that: The chromatographic column further comprises: A column tube, one end of which is connected to a liquid inlet tube, the other end of which is connected to the liquid inlet end of the chromatographic column, and the other end of which is connected to a liquid outlet tube, the other end of which is connected to the liquid outlet end of the chromatographic column; The filter part is placed in the column tube and fixedly connected to both ends of the column tube, and is used to filter impurities in the liquid and sample.

3. The liquid chromatograph with a multi-channel chromatographic column according to claim 2, characterized in that: The switching component includes: An upper liquid tube and a lower liquid tube, both of which are placed outside the column tube; A first valve body, which is used to control the on / off of the liquid inlet pipe; The second valve body is used to control the on-off of the upper liquid pipe; A third valve body, which is used to control the opening and closing of the liquid outlet pipe; The fourth valve body is used to control the opening and closing of the lower liquid pipe.

4. The liquid chromatograph with a multi-channel chromatographic column according to claim 3, characterized in that: When the first valve body is connected to the third valve body, the sample and the liquid pass through the liquid inlet tube, the column tube, and the liquid outlet tube in sequence to separate the sample; When the second valve body is connected to the fourth valve body, the liquid passes through the liquid inlet pipe, the upper liquid pipe, the column pipe, the lower liquid pipe and the liquid outlet pipe in sequence to perform backflushing cleaning of the chromatographic column.

5. The liquid chromatograph with a multi-channel chromatographic column according to claim 2, characterized in that: An end portion of the main flow channel extends to the inner side of the column tube, and an end portion of the secondary flow channel is close to the inner side of the column tube.

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