一种快速检测耐甲氧西林金黄色葡萄球菌的试纸条及其应用
By combining immunochromatographic technology and tyrosine signal amplification reaction with test strips coated with MoS2 nanosheets on the detection pad, the problems of time-consuming and complex existing MRSA detection and background fluorescence interference are solved, and rapid and sensitive MRSA detection is achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CHONGQING NO 3 PEOPLES HOSPITAL
- Filing Date
- 2025-03-05
- Publication Date
- 2026-07-17
AI Technical Summary
Existing MRSA detection methods are time-consuming and complex to operate, making it difficult to meet the needs of rapid on-site testing. Furthermore, paper-based sensors are prone to background fluorescence interference under excitation light, affecting the accuracy of the detection.
A rapid test strip for detecting methicillin-resistant Staphylococcus aureus was designed by using MoS2 nanosheets to quench background fluorescence on the detection pad and combining immunochromatography with tyrosine signal amplification. The MoS2 nanosheets quench background fluorescence to improve the signal-to-noise ratio, and the signal aggregation and amplification are achieved by using MoS2 nanosheets on the detection pad and horseradish peroxidase-labeled penicillin-binding protein 2a polyclonal antibody on the binding pad.
Rapid and sensitive detection of MRSA samples was achieved within 20 minutes, improving the sensitivity and accuracy of the detection and meeting the needs for portable and rapid on-site testing.
Smart Images
Figure CN120085001B_ABST
Abstract
Citation Information
Patent Citations
Antibody specific to methicillin resistant staphylococcus aureus, detection method and kit for methicillin resistant staphylococcus aureus using the same
CN102482346A
Aptamer based methicillin-resistant staphylococcus aureus fluorescence detection method
CN108982848A