Recombinant bacillus subtilis for exocrine expression of starvation element, method for producing starvation element through extracellular secretion expression and application of recombinant bacillus subtilis

Abstract

The invention belongs to the technical field of bioengineering, and discloses recombinant bacillus subtilis for expressing starvation element in an exosome manner, a method for producing the starvation element through extracellular secretion expression and application of the recombinant bacillus subtilis. The recombinant bacillus subtilis for exosome expression of the starvation element is composed of a recombinant plasmid and an expression host, and the recombinant plasmid comprises a starvation element target gene and a signal peptide gene; wherein the signal peptide gene is an RpmG signal peptide gene or an AspB signal peptide gene. By using the recombinant bacillus subtilis for exosome expression of the starvation element, the starvation element can be fermented and produced by utilizing the characteristic of exosome secretory expression, and the fermentation liquor can be used for preparing an animal feed additive with the function of improving the appetite or growth performance of animals. The method is suitable for industrial fermentation production of the starvation element.

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