High-efficiency culture device for trichogramma and culture method thereof

Abstract

The application belongs to the technical field of trichogramma breeding, and particularly relates to a high-efficiency culture device for bacteria-carrying trichogramma and a culture method thereof. The application comprises a box body, a moving plate arranged on the box body, a trichogramma breeding plate arranged with fixed trichogramma bodies, a liquid tank for containing liquid and conveying liquid or bacteria liquid, an intelligent control body for integrated control, and the like structures, and achieves the effects of high bacteria-carrying rate and high production efficiency.

Description

Technical Field

[0001] This invention belongs to the field of Trichogramma wasp cultivation technology, specifically relating to a highly efficient cultivation device and cultivation method for bacteria-carrying Trichogramma wasps. Background Technology

[0002] Trichogramma wasps are important parasitic natural enemy insects widely used in the biological control of agricultural pests. They control pest populations by laying their eggs inside the host insect's eggs, preventing the eggs from hatching. Trichogramma wasps are highly effective against lepidopteran pests such as corn borers and pine caterpillars, and have become one of the most widely used biological control methods globally.

[0003] However, the control effect of traditional Trichogramma wasps is limited by their single parasitic function; they can only control pest populations by parasitizing pest eggs and cannot directly kill pest larvae and adults. In addition, the parasitism efficiency of Trichogramma wasps is greatly affected by environmental factors such as temperature, humidity, and host egg quality, resulting in inconsistent effectiveness in practical applications.

[0004] To improve the control efficacy of Trichogramma wasps, technicians have developed "bacterial-carrying Trichogramma wasps." These wasps are artificially implanted with pathogenic microorganisms such as insect viruses, Beauveria bassiana, and Bt bacteria. The wasps introduce these pathogens into the pest population while searching for host eggs, achieving a dual control effect of "wasp killing eggs and microorganisms killing larvae." This technology not only improves the control efficacy of Trichogramma wasps but also significantly enhances their ability to sustainably control pest populations. However, current technologies struggle to ensure the efficient and stable carrying of pathogenic microorganisms by Trichogramma wasps, leading to unstable control effects. Furthermore, traditional methods for cultivating bacterial-carrying Trichogramma wasps rely on manual operation, which is inefficient and costly, making it difficult to meet the needs of large-scale production. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a highly efficient culture device and method for carrying bacteria-carrying Trichogramma wasps, characterized by high bacterial carrying rate and high production efficiency.

[0006] Specifically, a first aspect of the present invention is to provide a highly efficient culture device for carrying bacteria-carrying Trichogramma wasps. The box includes a body with an opening on one side and a dirt box connected to the bottom of the box; The main body includes two side walls, a fixed wall connecting the two side walls, a screen bottom plate and a top plate. The main body is equipped with a temperature controller, evenly distributed adjustable nozzles and a monitoring device set in the middle of the side walls. The side walls are provided with a moving channel, and the fixed wall is provided with a fixed cavity. A movable plate, which moves through a movable channel, and is opposite to a fixed wall; A bee-breeding board, comprising a movable plate and a fixed bee body, wherein the movable plate comprises a movable plate A and a movable plate B, the movable plate A and the movable plate B are connected by a snap-fit ​​structure, and the movable plate A and / or the movable plate B have evenly distributed holes, the fixed bee body comprises a bee-breeding area and a clamping area, and the bee-breeding board is movably inserted into a fixed cavity for fixation; The bee-breeding area is placed in the hole of the plate, and the clamping area is clamped and fixed by movable plate A and movable plate B. A liquid tank, which is connected to an adjustable nozzle via a pipe; An intelligent control unit, which can control adjustable nozzles and temperature regulators, includes an intelligent control system.

[0007] In some implementations, after the bee-breeding board is inserted into the fixed cavity for fixation, the monitoring device can fully monitor the bee-breeding board.

[0008] In some implementations, an exhaust fan and a waste bacterial liquid tank are also included, wherein the exhaust fan is controlled by an intelligent controller.

[0009] In some implementations, the bee-breeding area is loaded with 400 to 500 alternative host eggs per square centimeter for Trichogramma wasps.

[0010] In some implementations, the intelligent controller includes an intelligent control system.

[0011] In some implementations, the intelligent control system includes placing standard Trichogramma wasp cards from different stages into the device, monitoring the standard Trichogramma wasp cards through a monitoring device to obtain standard values, recording them to the intelligent control system, and then monitoring the development of the Trichogramma wasps during the development process using the monitoring device and comparing them with the standard values ​​to monitor the development of the Trichogramma wasps.

[0012] In some implementations, the standard values ​​include the color of the bee eggs and the plumpness of the eggs.

[0013] In some implementations, the intelligent control system also includes monitoring and control of the internal environment of the device.

[0014] A second aspect of the present invention provides a method for culturing Trichogramma wasps carrying bacteria, characterized in that the culturing method includes the following steps: A. Load the Trichogramma wasp eggs onto the breeding area of ​​the high-efficiency culture device for carrying Trichogramma wasps, fix them with a movable plate, and place them into the device; B. Add bacterial solution to the liquid tank, and spray it through the adjustable nozzle controlled by the intelligent controller. At the same time, the intelligent controller detects and controls the concentration of bacterial solution in the device. After spraying, use an exhaust fan to air dry it to remove excess water and ensure that the bacterial solution is effectively adsorbed. C. Once the embryos have reached the pre-pupae stage, stop spraying the bacterial solution to complete the preparation of the bacteria-carrying Trichogramma wasps. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a structural diagram of the present invention; Figure 2 This is a structural diagram of the box body in this invention; Figure 3 This is a schematic diagram of the bee-breeding board in this invention; Figure 4 This is a schematic diagram of the movable plate in this invention; Figure 5 This is a schematic diagram of the structure for fixing the bee body in this invention. Detailed Implementation

[0017] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0018] The components of the embodiments of the invention described and shown in the accompanying drawings can typically be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0019] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal connection of two components. For those skilled in the art, the specific meaning of the above terms in this invention can be understood according to the specific circumstances.

[0021] The first aspect of this invention is to provide a highly efficient culture device for carrying bacteria-carrying Trichogramma wasps. The box includes a body with an opening on one side and a dirt box connected to the bottom of the box; The main body includes two side walls, a fixed wall connecting the two side walls, a screen bottom plate and a top plate. The main body is equipped with a temperature controller, evenly distributed adjustable nozzles and a monitoring device set in the middle of the side walls. The side walls are provided with a moving channel, and the fixed wall is provided with a fixed cavity. A movable plate, which moves through a movable channel, and is opposite to a fixed wall; A bee-breeding board, comprising a movable plate and a fixed bee body, wherein the movable plate comprises a movable plate A and a movable plate B, the movable plate A and the movable plate B are connected by a snap-fit ​​structure, and the movable plate A and / or the movable plate B have evenly distributed holes, the fixed bee body comprises a bee-breeding area and a clamping area, and the bee-breeding board is movably inserted into a fixed cavity for fixation; The bee-breeding area is placed in the hole of the plate, and the clamping area is clamped and fixed by movable plate A and movable plate B. A liquid tank, which is connected to an adjustable nozzle via a pipe; The intelligent control unit can control the adjustable nozzle and temperature controller.

[0022] The above-mentioned information can achieve high bacterial load and high production efficiency.

[0023] The following description, in conjunction with figures, illustrates an exemplary embodiment of the efficient culture device and method for carrying Trichogramma hymenosa according to the present invention.

[0024] At least one embodiment of this disclosure provides a highly efficient culture device for carrying bacteria-carrying Trichogramma wasps. Figure 1 The diagram shows the structure of a highly efficient culture device for carrying Trichogramma wasps. Figure 1 The box 1 shown includes a body 11 with an opening on one side and a dirt box 6 connected to the bottom of the box 1. The main body 11 includes two side walls 111, a fixed wall 112 connecting the two side walls 111, a screen bottom plate 16, and a top plate 113. The main body 11 is equipped with a temperature controller 13, evenly distributed adjustable nozzles 17, and a monitoring device 14 set in the middle of the side walls 111. The monitoring device 14 can be a camera or an infrared imaging device, etc., and can be used to extract bee egg characteristic parameters. The side walls 111 are provided with a moving channel 18, and the fixed wall 112 is provided with a fixed cavity 15. In this embodiment, the temperature controller 13 can effectively regulate the internal temperature of the device, ensuring the normal reproduction of bee eggs. At the same time, the adjustable nozzle 17 can release the bacterial solution into the device by spraying, and can effectively regulate the bacterial concentration in the device. The survival rate of adult Trichogramma wasps and the activity parameters of the bacterial solution are monitored in real time. A critical concentration threshold is selected to ensure that the survival rate of Trichogramma wasps is ≥90% and the bacterial solution activity is maintained at ≥80%. The bacterial solution concentration is stabilized within ±5% of the critical concentration threshold by the feedback control system, ensuring that the bee eggs gradually adapt to the presence of the bacteria, effectively improving the coexistence with the bacteria, increasing the number of bacteria carried, and improving the cultivation method of bacteria-carrying Trichogramma wasps.

[0025] The movable plate 12 moves through the movable channel 18 and is opposite to the fixed wall 112; the main body 11 can be effectively opened or closed by moving the movable plate 12.

[0026] For example, Figure 3 A schematic diagram of the bee breeding board is shown. Figure 4 A schematic diagram of the movable plate is shown, combined with Figure 2 and Figure 2 The bee-breeding board 7 includes a movable plate 71 and a fixed bee body 72. The movable plate 71 includes a movable plate A711 and a movable plate B712, which are connected by a snap-fit ​​structure. For example, movable plate A711 has a snap head, and movable plate B712 has a snap groove. The two cooperate to achieve a good connection and simultaneously clamp the fixed bee body 72. The movable plate A711 and / or movable plate B712 have evenly distributed plate holes 713. Figure 5 This is a structural diagram of a fixed bee body, combined with... Figure 5 The fixed bee body 72 includes a bee-breeding area 721 and a clamping area 722. The bee-breeding plate 7 is movably inserted into the fixing cavity 15 for fixing. The solid bee body 72 is made of paper, transparent plastic, etc. The bee eggs are fixed in the bee-breeding area 721 with non-toxic glue.

[0027] For example, the bee-breeding area 721 is placed in the plate hole 713, and the clamping area 722 is clamped and fixed by the movable plate A711 and the movable plate B712; For example, such as Figure 1 The liquid tank 3 shown is connected to the adjustable nozzle 17 via a pipe; the liquid tank 3 can hold water or bacterial solution, and different concentrations of bacterial solution can be placed in it as needed to meet the requirements of bacterial solution concentration in the device.

[0028] For example, in some embodiments, such as Figure 1 The intelligent control unit 2 shown can control the adjustable nozzle 17 and the temperature controller 13.

[0029] For example, in some embodiments, after the bee-breeding board 7 is inserted into the fixing cavity 15 for fixing, the monitoring device 14 can fully monitor the bee-breeding board 7.

[0030] For example, in some embodiments, such as Figure 1 The exhaust fan 5 and waste liquid tank are shown. The exhaust fan 5 is controlled by the intelligent controller 2. The exhaust fan 5 can be used to control the dryness inside the device to ensure the normal hatching of bee eggs.

[0031] Therefore, as Figure 5 The bee-breeding area 721 shown is loaded with 400-500 alternative host eggs per square centimeter of Trichogramma wasps. Maintaining this density of load can effectively improve the production efficiency of Trichogramma wasps carrying the bacteria.

[0032] For example, in some embodiments, such as Figure 1 The intelligent control unit 2 shown includes an intelligent control system. The intelligent control system includes placing standard Trichogramma wasp cards of different stages into the device, monitoring the standard Trichogramma wasp cards through a monitoring device 14 to obtain standard values, and recording them to the intelligent control system. Subsequently, during the cultivation of Trichogramma wasps carrying the bacteria, the monitoring device 14 is used to monitor and compare the values ​​with the standard values ​​to monitor the development of the Trichogramma wasps carrying the bacteria. If the monitored value is lower than the standard value by a set minimum value, such as 80%, 85%, 90%, 95%, etc., the intelligent control unit 2 will issue an alarm to remind the user to check or adjust.

[0033] The standard values ​​mentioned here include the color of the bee eggs and the plumpness of the eggs.

[0034] For example, in some embodiments, the intelligent control system further includes monitoring and control of the internal environment of the device.

[0035] The presence of the intelligent control unit 2 in this disclosure ensures the cultivation and self-monitoring of bacteria-carrying Trichogramma wasps, reduces production costs, and improves cultivation results.

[0036] At least one embodiment of this disclosure provides a method for culturing Trichogramma wasps carrying bacteria, the method comprising the following steps: A. The Trichogramma wasp eggs are loaded into the breeding area 721 of the high-efficiency culture device for carrying Trichogramma bacteria, and fixed by the movable plate 71 before being placed into the device; B. Add bacterial solution to liquid tank 3, and control the adjustable nozzle 17 to spray through intelligent control unit 2. At the same time, intelligent control unit 2 detects and controls the concentration of bacterial solution in the device. After spraying, use exhaust fan 5 to air dry to remove excess water and ensure that the bacterial solution is effectively adsorbed. In this process, the bacteria used are bacteria that can kill or cause disease in pests, such as insect viruses, Beauveria bassiana, Bacillus thuringiensis, Metarhizium anisopliae, etc. C. Once the embryos have reached the pre-pupae stage, stop spraying the bacterial solution to complete the preparation of the bacteria-carrying Trichogramma wasps.

[0037] In the above scheme, the Trichogramma wasps that can be selected include Trichogramma pine caterpillar, Trichogramma corn borer, and Trichogramma moniliformis.

[0038] Using the aforementioned equipment and methods, such as Beauveria bassiana and Trichogramma pygmyces, the temperature is controlled at (26±1)℃, RH at (60±10)%, and the photoperiod is 14L:10D via intelligent control unit 2. The concentration of Beauveria bassiana bacterial solution is 10 in the first stage. 4 Spores / mL (spray three times at 3-hour intervals), Phase 2 10 5 Spores / mL (spray three times at 3-hour intervals), Stage 3 10 6 Spores / mL (spray three times at 3-hour intervals), Stage 4 10 7 The concentration of Beauveria bassiana spores (spores / mL) was determined in the fourth stage by spraying at 4-hour intervals, and monitored to a concentration of 10. 7 The spore count / mL was the critical concentration threshold. After maintaining this level and culturing to the prepupal stage, spraying of the bacterial solution was stopped. After the bee eggs emerged, the bacterial load and emergence rate were measured. The bacterial load was 4.26 × 10⁻⁶. 4 Each spore per bee has an emergence rate of over 91%.

[0039] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A highly efficient culture device for carrying bacteria, characterized in that, Box (1), the box (1) includes a body (11) with an opening on one side and a dirt box (6) connected to the bottom of the box (1). The main body (11) includes two side walls (111), a fixed wall (112) connecting the two side walls (111), a screen bottom plate (16), and a top plate (113). The main body (11) is provided with a temperature controller (13), evenly distributed adjustable nozzles (17), and a monitoring device (14) set in the middle of the side walls (111). The side walls (111) are provided with a moving channel (18) at one end, and the fixed wall (112) is provided with a fixed cavity (15). A movable plate (12) is moved through a movable channel (18) and is opposite to a fixed wall (112); The bee-breeding board (7) includes a movable plate (71) and a fixed bee body (72). The movable plate (71) includes a movable plate A (711) and a movable plate B (712). The movable plate A (711) and the movable plate B (712) are connected by a snap-fit ​​structure. The movable plate A (711) and / or the movable plate B (712) have evenly distributed plate holes (713). The fixed bee body (72) includes a bee-breeding area (721) and a clamping area (722). The bee-breeding board (7) is movably inserted into a fixed cavity (15) for fixation. The bee-breeding area (721) is placed in the plate hole (713), and the clamping area (722) is clamped and fixed by the movable plate A (711) and the movable plate B (712). Liquid tank (3), which is connected to adjustable nozzle (17) via a pipe; The intelligent control unit (2) can control the adjustable nozzle (17), the temperature controller (13) and the exhaust fan (5), and the intelligent control unit (2) is used to detect and control the concentration of bacterial liquid in the device. The intelligent control unit (2) includes an intelligent control system. The intelligent control system includes placing standard Trichogramma bee cards of different periods into the device, monitoring the standard Trichogramma bee cards through the monitoring device (14) to obtain standard values, recording them to the intelligent control system, and then using the monitoring device (14) to monitor the Trichogramma bee-carrying process during the cultivation process, comparing them with the standard values, and monitoring the development of the Trichogramma bee-carrying process. When the comparison result is lower than the preset threshold, an alarm signal is issued.

2. The high-efficiency culture device for carrying bacteria, *Trichogramma rubescens*, according to claim 1, is characterized in that... After the bee-breeding board (7) is inserted into the fixed cavity (15) for fixation, the monitoring device (14) can fully monitor the bee-breeding board (7).

3. The high-efficiency culture device for carrying bacteria, *Trichogramma rubescens*, according to claim 1, is characterized in that... It also includes a waste bacterial solution tank, which is used to store waste bacterial solution.

4. The high-efficiency culture device for carrying bacteria, *Trichogramma hymenopsii*, according to claim 1, is characterized in that... The bee-breeding area (721) is loaded with 400-500 alternative host eggs per square centimeter for Trichogramma wasps.

5. The high-efficiency culture device for carrying bacteria, *Trichogramma rubescens*, according to claim 1, is characterized in that... The standard values ​​include the color of the bee eggs and the plumpness of the eggs.

6. The high-efficiency culture device for carrying bacteria, *Trichogramma rubescens*, according to claim 1, is characterized in that... The intelligent control system also includes monitoring and control of the internal environment of the device.

7. A method for culturing a bacteria-carrying Trichogramma wasp, characterized in that, The culture method includes the following steps: A. Load the Trichogramma wasp eggs into the breeding area (721) of the high-efficiency culture device for carrying bacteria Trichogramma wasps as described in any one of claims 1-6, and fix them with a movable plate (71) and place them into the device; B. Add bacterial solution to liquid tank (3), and spray the adjustable nozzle (17) through intelligent control body (2). At the same time, intelligent control body (2) detects and controls the concentration of bacterial solution in the device. After spraying, air dry it through exhaust fan (5) to remove excess water and ensure that the bacterial solution is effectively adsorbed. C. Once the fungal culture reaches the pre-pupa stage, stop spraying the bacterial solution to complete the culture of the bacteria-carrying Trichogramma wasps.

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