A method for algae removal in urban water bodies based on bottom sediment microorganisms

Abstract

This invention discloses a method for algae removal in urban water bodies based on microorganisms in bottom sediment. The method specifically includes the following steps: S1, obtaining bottom sediment from the water body to be treated and refrigerating the sediment; S2, obtaining algae samples from the water body to be treated and pre-treating the algae samples; S3, preparing a culture medium and preparing an algae-removing culture solution according to the prepared culture medium; S4, diluting the algae-removing culture solution according to the area of ​​the water body to be treated, obtaining the diluted algae-removing culture solution, and uniformly spraying the diluted algae-removing culture solution into the water body to be treated, thus completing the algae removal. This invention utilizes bottom sediment microbial resources as a resource pool, screening for microbial communities that are highly effective at removing green algae from the water body, using locally sourced materials, and is simple and convenient to quickly solve the eutrophication problem in urban park ponds.

Description

Technical Field

[0001] This invention belongs to the field of water pollution control technology, specifically relating to a method for removing algae from urban water bodies based on microorganisms in bottom sediment. Background Technology

[0002] Urban ecological construction is a crucial guarantee for urban development, and urban landscaping is an important component of urban ecological construction. Artificial wetlands are a vital carrier of urban landscape and ecological functions within urban landscaping. Urban artificial wetlands are generally small rivers, ponds, or streams, and are typically static water bodies. Due to the daily use of vehicles such as cars and ships, water bodies, soil, and air are polluted to varying degrees, especially by heavy metals and organic pollutants. These pollutants directly enter artificial wetlands in large quantities. Simultaneously, excessive application of fertilizers during landscaping maintenance accelerates algae growth in the water. These factors combined cause the slow deterioration of water quality in park artificial wetlands, severely impacting the aquatic landscape and its ecological functions.

[0003] In existing technologies, the content of green algae in park water bodies or aquaculture water bodies is too high, and the water bodies appear obviously dark green or oily green. Although park water bodies are often manually dredged to remove a large number of filamentous algae and large algae, a large number of tiny single-celled or multi-celled green algae still remain in the water bodies, affecting the water quality of park water bodies. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a method for removing algae from urban water bodies based on microorganisms in bottom sediment. This method uses microbial resources from bottom sediment as a resource pool, selects microbial groups that are suitable for the efficient removal of green algae in the water body, uses local materials, is simple to operate, and can quickly and easily solve the problem of eutrophication in urban park ponds.

[0005] To achieve the above-mentioned objectives, the technical solution provided by this invention patent is as follows:

[0006] A method for algae removal in urban water bodies based on microorganisms in bottom sediments, the method specifically includes the following steps:

[0007] S1, Obtain the bottom sediment from the water body to be treated for algae removal, and refrigerate the bottom sediment;

[0008] S2, Obtain algae samples from the water body to be treated and preprocess the algae samples;

[0009] S3, Prepare the culture medium, and prepare the algae removal culture solution according to the prepared culture medium;

[0010] S4. Dilute the algae removal culture solution according to the area of ​​the water body to be treated, obtain the diluted algae removal culture solution, and spray the diluted algae removal culture solution evenly into the water body to be treated, thus completing the algae removal.

[0011] Furthermore, the collected bottom sediment is placed in a sampling bag and refrigerated at a temperature of 0-5℃.

[0012] Furthermore, the algae sample acquisition process specifically involves: taking 100L of water to be treated as the sample water, which contains aquatic algae, filtering the sample water, and obtaining all the algae in the sample water as the algae sample.

[0013] Furthermore, the pretreatment of the algae samples specifically involves: evenly distributing the obtained algae samples on filter paper with a diameter of 9 cm, and then dividing the filter paper into four equal parts to obtain multiple algae samples.

[0014] Furthermore, the culture medium is specifically prepared as follows:

[0015] S31, extract water from the water body to be de-algaed and divide it into multiple sample water bodies, and add refrigerated bottom mud to each sample water body;

[0016] S32, after adding bottom sediment to the sample water, algae samples are added. After adding algae samples, the sample water is shaken at room temperature to cultivate the culture medium.

[0017] Furthermore, the volume of the sample water is 100 ml, and the mass of the bottom sediment added to the sample water is 5-10 g.

[0018] Furthermore, before adding the algae sample to the sample water, the filter paper is cut into small pieces and then added to the sample water.

[0019] Furthermore, the culture medium is specifically cultured as follows: after the culture medium is prepared, once the culture medium becomes turbid and the green color of the culture medium becomes lighter or disappears, the sample water in the culture medium is transferred to fresh culture medium for culture to obtain a culture solution.

[0020] Based on the above technical solution, the method for algae removal in urban water bodies based on microorganisms in bottom sediment of this invention has achieved the following technical advantages through practical application:

[0021] 1. The present invention provides a method for removing algae from urban water bodies based on microorganisms in bottom sediment. This method uses microbial resources from bottom sediment as a resource pool, selects microbial groups that are suitable for the efficient removal of green algae in the water body, uses locally sourced materials, is simple to operate, and is convenient and quick to solve the eutrophication phenomenon in urban park ponds. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this invention clearer, specific examples are described below. However, it should be understood that these descriptions are merely exemplary and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and techniques are omitted in the following description to avoid unnecessarily obscuring the concept of the invention.

[0023] This invention relates to a method for algae removal in urban water bodies based on microorganisms in bottom sediments. The method specifically includes the following steps:

[0024] S1, Obtain the bottom sediment from the water body to be treated for algae removal, and refrigerate the bottom sediment;

[0025] S2, Obtain algae samples from the water body to be treated and preprocess the algae samples;

[0026] S3, Prepare the culture medium, and prepare the algae removal culture solution according to the prepared culture medium;

[0027] S4. Dilute the algae removal culture solution according to the area of ​​the water body to be treated, obtain the diluted algae removal culture solution, and spray the diluted algae removal culture solution evenly into the water body to be treated, thus completing the algae removal.

[0028] The collected bottom sediment was placed in a sampling bag and refrigerated at a temperature of 0-5℃.

[0029] The algae sample acquisition process is as follows: 100L of water to be treated with algae is taken as the sample water. The sample water contains aquatic algae. The sample water is filtered to obtain all the algae in the sample water, which is the algae sample.

[0030] The pretreatment of the algae samples specifically involves: evenly distributing the obtained algae samples on filter paper with a diameter of 9 cm, and then dividing the filter paper into four equal parts to obtain multiple algae samples.

[0031] The culture medium is specifically prepared as follows:

[0032] S31, extract water from the water body to be de-algaed and divide it into multiple sample water bodies, and add refrigerated bottom mud to each sample water body;

[0033] S32, after adding bottom sediment to the sample water, algae samples are added. After adding algae samples, the sample water is shaken at room temperature to cultivate the culture medium.

[0034] The volume of the sample water is 100ml, and the mass of the bottom sediment added to the sample water is 5-10g.

[0035] Before adding the algae sample to the water sample, the filter paper was cut into small pieces.

[0036] The culture medium is prepared as follows: after the culture medium is prepared, wait until the culture medium becomes turbid and the green color of the culture medium becomes lighter or disappears, then transfer the sample water in the culture medium to fresh culture medium to obtain culture solution.

[0037] This invention utilizes the abundant microbial resources in natural sediments to develop green algae-degrading bacteria, which not only enriches the variety of green algae-degrading bacteria on the market, but also effectively inhibits the excessive proliferation of algae in the static water of urban park ponds.

[0038] In this patent application, the dilution ratio of the algae-removing culture medium is not determined solely based on the water area; other factors, including but not limited to the degree of pollution of the water body to be algaed, the type and density of algae, water temperature, light conditions, and the expected algae removal effect, determine the dilution ratio of the algae-removing culture medium.

[0039] Specifically, a base dilution ratio (e.g., 1:100) is set, and then adjusted appropriately according to the size of the water area and other factors mentioned above. For example, for smaller water areas, the base dilution ratio is selected for spraying or spraying at a value close to the base dilution ratio. For larger and more polluted water areas, the dilution ratio is reduced (i.e., the concentration of the algae-removing culture solution is increased) to improve algae removal efficiency.

[0040] Example

[0041] Take a pond in a park of a certain research institute as an example;

[0042] 1. Use a grab bucket to collect 1 kg of algae-containing pond bottom mud from a park within a research institute, place it in a sampling bag, and refrigerate it at 0-5℃.

[0043] 2. Take 100L of algae-containing water from the pond in step 1, filter it using filter paper with a diameter of 9 cm, and the green algae will be evenly distributed on the filter paper. Cut the filter paper into 4 equal parts.

[0044] 3. Preparation of microbial culture medium: The culture medium includes the following components:

[0045] 5g of pond bottom mud from step 1;

[0046] After dividing the filter paper into equal portions in step 2 and cutting the filter paper into small pieces;

[0047] The pond water containing algae in step 1 contains 100ml;

[0048] Mix the algae-containing water, bottom sediment, and shredded filter paper; incubate at room temperature with shaking; when the culture medium becomes turbid and the green color lightens or disappears, transfer it to fresh culture medium for further incubation.

[0049] To increase the culture volume, the number of culture bottles can be increased as needed. The culture medium should be collected for later use and care should be taken to preserve the culture. The culture medium contains a large population of microorganisms that decompose green algae;

[0050] 4. Dilute the culture medium obtained in step 3 by 100-500 times and spray it evenly into the pond water. Regularly monitor the water quality of the pond.

[0051] The small wetland in a park of a certain research institute consists of three small ponds, namely the south, east and north sides, which are interconnected. The experiment was conducted in the south and north ponds. The algae-removing bacteria were prepared according to steps 1-4. The obtained algae-removing bacteria solution was diluted 100 times and sprayed on the south and north ponds respectively. The middle east pond was used as a control. The results were observed after 30 days.

[0052]

[0053] As shown in the table above, the CO and chlorophyll contents in the south side of the pond sprayed with algae-removing bacterial solution and the tested pond were significantly lower than those in the east side pond that was not sprayed with algae-removing bacterial solution, indicating that the algae removal method of the present invention has the function of algae removal. The present invention utilizes the abundant microbial resources in the bottom sediment of nature to develop green algae-degrading bacteria, which not only enriches the types of green algae-degrading bacteria on the market, but also effectively inhibits the excessive proliferation of algae in the static water of urban park ponds. The corresponding bacterial solution is cultivated for urban park ponds in different areas, which improves the targeting of algae removal.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can still be made to the specific implementation of the invention or equivalent substitutions can be made to some technical features without departing from the spirit of the technical solutions of the present invention, and all such modifications and substitutions should be covered within the scope of the technical solutions claimed in the present invention.

Claims

1. A method for removing algae from urban water bodies based on sediment microorganisms of water bodies, characterized in that, The method specifically includes the following steps: S1, Obtain the bottom sediment from the water body to be treated for algae removal, and refrigerate the bottom sediment; S2, Obtain algae samples from the water body to be treated and preprocess the algae samples; The algae sample is obtained as follows: 100L of water to be de-algaed is taken as the sample water. The sample water contains aquatic algae. The sample water is filtered to obtain all the algae in the sample water, which is the algae sample. The pretreatment of the algae samples specifically involves: evenly distributing the obtained algae samples on filter paper with a diameter of 9 cm, then dividing the filter paper into four equal parts to obtain multiple algae samples; S3, Prepare the culture medium, and prepare the algae removal culture solution according to the prepared culture medium; The culture medium is specifically prepared as follows: S31, extract water from the water body to be de-algaed and divide it into multiple sample water bodies, and add refrigerated bottom mud to each sample water body; S32, after adding bottom sediment to the sample water, pretreated algae samples are added, and then the sample water is shaken at room temperature for culture. The culture medium is specifically prepared as follows: after the culture medium is prepared, wait until the culture medium becomes turbid and the green color of the culture medium becomes lighter or disappears, then transfer the sample water in the culture medium to a fresh culture medium to obtain an algae removal culture solution. S4. Dilute the algae removal culture solution according to the area of ​​the water body to be algaed, obtain the diluted algae removal culture solution, and spray the diluted algae removal culture solution evenly into the water body to be algaed to complete the algae removal.

2. The method for removing algae from urban water bodies based on microorganisms in water body sediment according to claim 1, characterized in that, The obtained bottom sediment is placed in a sampling bag and refrigerated at a temperature of 0-5℃.

3. The method for removing algae from urban water bodies based on microorganisms in water body sediment according to claim 1, characterized in that, The volume of the sample water in S31 is 100ml, and the mass of the bottom sediment added to the sample water is 5-10g.

4. The method for algae removal in urban water bodies based on bottom sediment microorganisms according to claim 1, characterized in that, Before adding the algae sample to the water sample, the filter paper was cut into small pieces.

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