An up-and-down flow system for artificial breeding of amphioxus and a breeding method of amphioxus

Abstract

The application discloses a lift flow system for artificial breeding of amphioxus and a breeding method of amphioxus, and belongs to the technical field of amphioxus breeding. The lift flow system comprises a breeding pool, a water inlet pipe, a rotating shaft and a water outlet. The system discharges the dirt at the bottom of the pool through the downflow, discharges the dirt in the water body through the upflow, and prevents the formation of algal film on the surface of the sand layer by rotating the sand layer through the rotating shaft. The system can effectively remove the residual feed and excrement in the pool bottom and the sand layer, effectively increase the water permeability of the sand layer through the reverse osmosis effect of the upflow, prevent the formation of algal film on the surface of the sand layer, reduce the ammonia nitrogen content in the water body, increase the dissolved oxygen content, provide a suitable environment for the growth of amphioxus, and improve the growth speed and survival rate of amphioxus.

Description

Technical Field

[0001] This invention belongs to the field of amphioxus breeding technology, specifically relating to an upflow / downflow system for artificial breeding of amphioxus and a breeding method for amphioxus. Background Technology

[0002] Amphioxus belongs to the phylum Chordata, subphylum Cephalochordate, class Cephalochordate, and family Branchiostomidae. As a transitional group between invertebrates and vertebrates, amphioxus are often referred to as living fossils and are important models for studying the origin and evolution of vertebrates. Amphioxus burrow in sand and have high requirements for their ecological environment, needing both suitable substrate and live food, as well as clean environmental conditions. In the artificial breeding of amphioxus, a layer of fine sand is usually laid to meet their living requirements. However, uneaten food and excrement easily accumulate on the surface of the sand layer, forming an algal film that causes the sand layer to compact. At the same time, harmful substances such as ammonia nitrogen in the water also increase, leading to a decrease in the survival rate and growth rate of amphioxus. Frequent replacement of the sand layer can easily damage the amphioxus, increasing the risk of death. Summary of the Invention

[0003] To address the problem that uneaten food and excrement tend to accumulate on the surface of sand layers in existing technologies, a lift-flow system for artificial breeding of amphioxus and a breeding method for amphioxus are provided.

[0004] A lift-flow system for the artificial cultivation of amphioxus includes a cultivation tank and an inlet pipe. The support frame is located at the bottom of the cultivation tank. A layer of hollow bricks is placed on the support frame, a layer of sieve cloth is placed on top of the hollow bricks, and a layer of sand is laid on top of the sieve cloth. The water inlet pipe is equipped with an upper water inlet and a lower water inlet that are connected to the upper and lower parts of the cultivation tank, respectively. The cultivation tank is also equipped with an upper water outlet and a lower water outlet.

[0005] The lifting and lowering system for artificial breeding of amphioxus also includes a rotating shaft, which is rotatably installed in the breeding pond and buried in the sand layer. The rotating shaft is driven to rotate by a motor installed outside the breeding pond, and the rotating shaft is provided with several protrusions.

[0006] The lifting and lowering system for artificial breeding of amphioxus includes a sealing block fitted on a rotating shaft and fixed to the breeding tank by bolts, a first sealing strip between the sealing block and the breeding tank, a second sealing strip between the sealing block and the rotating shaft, a bearing seat installed on the breeding tank, and a motor installed on the bearing seat.

[0007] The lifting and lowering system for artificial breeding of amphioxus has an annular groove on the end face of the sealing block for installing the first sealing strip.

[0008] The lifting and lowering system for artificial breeding of amphioxus has a V-shaped groove in the inner hole of the sealing block for installing a second sealing strip.

[0009] A method for cultivating amphioxus, using the aforementioned upflow / downflow system for artificial cultivation of amphioxus, comprises the following steps: S1. In the cultivation tank of the riser-faller system, a layer of hollow bricks, a layer of silk cloth, and a layer of sand are laid in sequence; and filtered fresh seawater is injected. S2. The hatched amphioxus larvae are placed in the cultivation pond of the riser system for cultivation at a stocking density of 100-160 fish / L; golden algae are fed twice a day; the water is changed once a day. The specific operation is as follows: between the two feedings, the upper inlet (21) and the lower outlet (32) are opened, the water exchange speed is 1-3 cm / s, the inlet speed is the same as the outlet speed, and the rotating shaft (4) is started to rotate at a speed of 5-10 r / min; the water exchange operation is carried out for 10-15 minutes. S3. When it is observed that the larvae of the amphioxus begin to burrow into the sand, change the water once a day. The specific operation is as follows: between two feedings, open the upper water inlet (21) and the lower water outlet (32), change the water at a speed of 1-3 cm / s, with the water inlet speed being the same as the water outlet speed, and perform the water change operation for 10-20 minutes without starting the rotating shaft (4). S4. After the amphioxus has finished burrowing in the sand, feed it a mixture of golden algae and hornwort twice a day; change the water twice a day. The specific operation is as follows: the first water change is between the two feedings, open the lower inlet (22) and the upper outlet (31), the water change speed is 2-5cm / s, the inlet speed is the same as the outlet speed, and start the rotating shaft (4) to rotate, the rotation speed of the rotating shaft (4) is 5-15r / min; perform the water change operation for 10-30min; the second water change is 6 hours after the second feeding, open the upper inlet (21) and the lower outlet (32), the water change speed is 1-3cm / s, the inlet speed is the same as the outlet speed, and perform the water change operation for 10-30min.

[0010] Based on the above scheme, the stocking density of Chinese bream larvae in step S1 is 140 fish / L.

[0011] Based on the above scheme, the water exchange rate in step S3 is 2 cm / s, and the water exchange time is 15 min.

[0012] Based on the above scheme, the first water change rate in step S4 is 4 cm / s, and the water change time is 15 min; the second water change rate is 2 cm / s, and the water change time is 30 min.

[0013] Based on the above scheme, the rotational speed of the shaft in step S4 is 10 r / min.

[0014] The beneficial effects of this invention are: This invention provides a rising and falling flow system for the artificial breeding of amphioxus. The system discharges waste from the bottom of the pond through a downward flow and discharges waste from the water through an upward flow. It also loosens the sand layer by rotating a shaft to prevent the formation of algae film on the surface of the sand layer.

[0015] The system of this invention can effectively remove uneaten food and excrement from the bottom of the pond and the sand layer. Through the upward flow reverse osmosis, it can effectively increase the permeability of the sand layer, prevent the formation of algal film on the sand layer surface, reduce the ammonia nitrogen content in the water, increase the dissolved oxygen content, provide a suitable environment for the growth of amphioxus, and improve the growth rate and survival rate of amphioxus.

[0016] This invention also provides a method for cultivating amphioxus using an upflow and downflow system. This method is simple to operate, eliminates the need for frequent sand layer replacement, and reduces the risk of death due to injury to the amphioxus. The combination of upflow and downflow in the upflow and downflow system effectively prevents algae film formation on the sand surface, increases sand permeability, and removes excrement from the pond bottom and sand layer. By setting the water flow speed and shaft rotation speed during water changes, the stress response of amphioxus can be reduced while lowering the levels of harmful substances such as ammonia nitrogen in the water, ensuring the survival rate and growth rate of the amphioxus. This method has significant application prospects in amphioxus cultivation. Attached Figure Description

[0017] Figure 1 This is a structural schematic diagram of an embodiment of the present invention; Figure 2 for Figure 1 Enlarged view of section A in the image. Detailed Implementation

[0018] To make the technical problems solved by the present invention, the technical solutions adopted, and the technical effects achieved clearer, the technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining the present invention and are not intended to limit the present invention. Furthermore, it should be noted that, for ease of description, only the parts related to the present invention are shown in the accompanying drawings, not all of them.

[0019] In the description of this invention, it should be noted that the terms "upper", "lower", "left", "right", "clockwise" and "counterclockwise" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting this invention. Example

[0020] An upflow / downflow system for the artificial breeding of amphioxus (see...) Figure 1 - Figure 2 The device includes a cultivation tank 1, an inlet pipe 2, and a rotating shaft 4. A support 11 is located at the bottom of the cultivation tank 1. A hollow brick layer 12 is installed on the support 11. A layer of silk cloth 13 is covered on top of the hollow brick layer 12. A sand layer 14 is then laid on top of the silk cloth layer 13. The rotating shaft 4 is rotatably installed in the cultivation tank 1 and is buried in the sand layer 14. The rotating shaft 4 is driven to rotate by a motor 5 installed outside the cultivation tank 1. Several protrusions 41 are provided on the rotating shaft 4.

[0021] The water inlet pipe 2 is provided with an upper water inlet 21 and a lower water inlet 22 that are respectively connected to the upper and lower parts of the cultivation tank 1. The cultivation tank 1 is also provided with an upper water outlet 31 and a lower water outlet 32. Water valves are provided on both the upper water inlet and the lower water outlet.

[0022] like Figure 2 As shown, the sealing block 6 is fitted onto the rotating shaft 4 and fixedly installed on the cultivation tank 1 with bolts. The first sealing strip 61 is disposed between the sealing block 6 and the cultivation tank 1, and the second sealing strip 62 is disposed between the sealing block 6 and the rotating shaft 4. The bearing seat 42 is installed on the cultivation tank 1, and the motor 5 is installed on the bearing seat 42.

[0023] An annular groove is provided on the end face of the sealing block 6 for installing the first sealing strip 61.

[0024] A V-shaped groove is provided in the inner hole of the sealing block 6 for installing the second sealing strip 62.

[0025] The sealing block 6, the first sealing strip 61, and the second sealing strip 62 can achieve a sealing effect and prevent water leakage.

[0026] The other end of the rotating shaft 4 is also equipped with a sealing block, sealing strip, and bearing housing, such as... Figure 1 As shown.

[0027] Amphioxus are typically cultured in sand. The disadvantage of this method is that as the culture time increases, the amount of uneaten food and excrement in the bottom of the pond and the sand layer increases, leading to a continuous increase in the content of bacteria, ammonia nitrogen, and nitrite, which threatens the growth and survival of the amphioxus. On the other hand, the accumulation of uneaten food can also cause algae to form on the surface of the sand layer, which is not conducive to the permeability of the sand layer and affects the growth and survival of the amphioxus.

[0028] This invention removes residual bait and excrement from sand layers via upwelling. Specific methods include... Figure 1As shown, the upper inlet 21 is closed, the lower inlet 22 is open for water supply, the lower outlet 32 ​​is closed, and the upper outlet 31 is open for water discharge. Thus, water flows from bottom to top through the hollow brick layer 12, the silk cloth layer 13, and the sand layer 14, and is discharged through the upper outlet 31. During the infiltration process, the water also discharges residual bait and excrement from the sand layer.

[0029] Uneaten food and excrement on the surface of the sand layer easily form an algal film, which cannot be broken down by water flow alone, resulting in incomplete discharge. Therefore, during the upflow discharge process, the rotating shaft 4 rotates, and the protrusion 41 slightly agitates the sand layer. As the sand layer loosens, the algal film formed on the surface of the sand layer can be effectively broken down, making it easier to discharge.

[0030] This invention removes waste from the bottom of the pool through a downward flow. Specific methods include... Figure 1 As shown, the upper inlet is open, the lower inlet 22 is closed, the upper outlet 31 is closed, and the lower outlet 32 ​​is open. Water flows in from above, passes through the sand layer, the silk cloth layer, and the hollow brick layer in sequence, and finally exits from the lower outlet 32, removing the sludge from the bottom of the pool. Example

[0031] The steps for cultivating amphioxus using an upflow / downflow system are as follows: S1. In the cultivation tank of the riser-faller system of Example 1, a hollow brick layer, a silk screen layer, and a sand layer are laid in sequence; and filtered fresh seawater is injected. The hollow brick layer is 5-10cm high, the silk screen layer has a pore size of 200-300 mesh, the sand layer has a sand particle size of 0.1-0.5mm, and the sand layer is 5-10cm thick.

[0032] S2. Place the hatched amphioxus larvae into the rearing tank of the aforementioned upflow and downflow system for cultivation at a stocking density of 100-160 larvae / L; feed them with golden algae twice a day (6:00 AM and 6:00 PM). Change the water once a day. Specifically, between feeding sessions, open the upper inlet 21 and the lower outlet 32, changing the water at a rate of 1-3 cm / s, with the inflow and outflow rates being the same, and start the rotating shaft 4 to slowly rotate at a speed of 5-10 r / min; perform the water change for 10-15 minutes.

[0033] S3. When it is observed that the larvae of the amphioxus begin to burrow into the sand, change the water once a day. The specific operation is as follows: between two feedings, open the upper water inlet 21 and the lower water outlet 32, change the water at a speed of 1-3 cm / s, with the water inlet speed being the same as the water outlet speed, and perform the water change operation for 10-20 minutes without starting the rotating shaft 4.

[0034] S4. After the amphioxus have completed their burrowing in the sand, feed them a mixture of golden algae and chamomile twice a day (6:00 AM and 6:00 PM). Change the water twice a day. Specifically: the first water change is between feedings (12:00 PM), opening the lower inlet 22 and upper outlet 31 at a rate of 2-5 cm / s, with the inflow and outflow rates matching. Simultaneously, start the rotating shaft 4 and slowly rotate it at 5-15 rpm for 10-30 minutes. The second water change is 6 hours after the second feeding (10:00 PM), opening the upper inlet 21 and lower outlet 32 ​​at a rate of 1-3 cm / s, with the inflow and outflow rates matching, for 10-30 minutes. Once the amphioxus have reached a suitable size, release them back into the stock. Example

[0035] The steps for cultivating amphioxus using an upflow / downflow system are as follows: S1. In the cultivation tank of the riser-fall system of Example 1, a hollow brick layer, a silk screen layer, and a sand layer are laid in sequence; and filtered fresh seawater is injected. The hollow brick layer is 8cm high, the silk screen layer has a pore size of 250 mesh, the sand layer has a sand particle size of 0.1-0.5mm, and the sand layer is 8cm thick.

[0036] S2. The hatched amphioxus larvae are placed into the rearing tank of the aforementioned upflow and downflow system at a stocking density of 140 larvae / L. They are fed with golden algae twice a day (6:00 AM and 6:00 PM). The water is changed once a day. Specifically, between feeding sessions, the upper inlet 21 and the lower outlet 32 ​​are opened, and the water exchange rate is 2 cm / s, with the inflow and outflow rates being the same. The rotating shaft 4 is then started and slowly rotated at a speed of 5 r / min. The water exchange operation lasts for 10 minutes.

[0037] S3. When it is observed that the larvae of the amphioxus begin to burrow into the sand, change the water once a day. The specific operation is as follows: between two feedings, open the upper water inlet 21 and the lower water outlet 32, change the water at a speed of 2cm / s, with the water inlet speed being the same as the water outlet speed, and perform the water change operation for 15 minutes without starting the rotating shaft 4.

[0038] S4. After the amphioxus have completed their burrowing in the sand, feed them a mixture of golden algae and chamomile twice a day (6:00 AM and 6:00 PM). Change the water twice a day. Specifically: the first water change is between feedings (12:00 PM), opening the lower inlet 22 and upper outlet 31 at a rate of 4 cm / s, with the inflow and outflow rates matching. Simultaneously, start the rotating shaft 4 and slowly rotate it at 10 rpm for 15 minutes. The second water change is 6 hours after the second feeding (10:00 PM), opening the upper inlet 21 and lower outlet 32 ​​at a rate of 2 cm / s, with the inflow and outflow rates matching. This water change lasts for 30 minutes. Once the amphioxus have reached a suitable size, release them back into the stock.

[0039] Based on Example 3, the stocking density in step S2 was set to 100 fish / L, 120 fish / L, 140 fish / L, and 160 fish / L, respectively; all other conditions remained unchanged. The ammonia nitrogen (NH3-N) and dissolved oxygen (DO) content in the culture water before daily feeding of the juveniles before they began burrowing into the sand were determined using Nessler's reagent spectrophotometry and the Winkler iodometric method. Each group was tested three times over three consecutive days, and the average value was calculated. The results are shown in Table 1.

[0040] Table 1. The impact of stocking density of amphioxus fry on water quality.

[0041] Table 1 shows that the stocking density of amphioxus fry has a certain impact on the ammonia nitrogen and dissolved oxygen content of the aquaculture water. As the stocking density increases, the ammonia nitrogen content gradually increases, while the dissolved oxygen content gradually decreases. Amphioxus have high requirements for water quality, especially juveniles. Therefore, in order to ensure the quality of the aquaculture water, the stocking density of amphioxus should not exceed 140 fish / L.

[0042] Based on Example 3, the water exchange rate during the amphioxus burrowing process in step S3 was set to 1 cm / s, 2 cm / s, and 3 cm / s, respectively; the water exchange duration for each rate was set to 10 min, 15 min, and 20 min; and a control group was set with a water exchange rate of 2 cm / s, a duration of 15 min, and the rotating shaft 4 was started to rotate (5 r / min). The survival rate of the amphioxus larvae was statistically analyzed, and the results are shown in Table 2.

[0043] Table 2. Effects of water change method on juvenile amphioxus survival rate during burrowing in the sand.

[0044] Table 2 shows that when the water exchange rate is 1 cm / s, the survival rate of burrowing larvae increases with the increase of water exchange time. At this speed, the water exchange rate is relatively slow, and the stress on the larvae is not significant. With the increase of water exchange time, the ammonia nitrogen content in the water decreases significantly, which is beneficial to the survival of the larvae. When the water exchange rate is 2 cm / s, the survival rate of burrowing larvae is relatively high. At this speed, the water exchange rate is relatively gentle, and short-term water exchanges have little impact on the survival rate of the larvae. When the water exchange time is 20 minutes, the survival rate of the larvae decreases slightly. When the water exchange rate is 3 cm / s, the water flow velocity is relatively high, which also causes greater stress on the burrowing larvae of the larvae. The survival rate of the larvae decreases significantly, and this decrease continues with the increase of water exchange time. When the water change rate is 2 cm / s and the water change time is 15 min, and the rotating shaft 4 is started to rotate during the water change, the survival rate of the amphioxus drops significantly to only 31.72%. This is because the rotation of the shaft disturbs the amphioxus's burrowing in the sand, causing greater stress to the amphioxus and thus significantly reducing the survival rate.

[0045] Based on Example 3, the water change in step S4 is carried out in the following manner: ① Change the water once a day. The specific operation is as follows: Change the water between two feedings (12:00 noon), open the lower water inlet 22 and the upper water outlet 31, change the water at a speed of 4cm / s, the water inlet speed is the same as the water outlet speed, and start the rotating shaft 4 to rotate slowly at a speed of 10r / min; change the water for 15 minutes.

[0046] ② Change the water once a day. The specific operation is as follows: Change the water 6 hours after the second feeding (at 22:00 in the evening), open the upper water inlet 21 and the lower water outlet 32, change the water at a speed of 2cm / s, with the water inlet speed being the same as the water outlet speed, and perform the water change operation for 30 minutes.

[0047] ③ During the first water change, the rotation speed of the shaft 4 is 5 r / min; the rest is the same as in Example 3.

[0048] ④ During the first water change, the rotation speed of the shaft 4 is 15 r / min; the rest is the same as in Example 3.

[0049] Nessler's reagent spectrophotometry and Winkler's iodometric titration method were used to detect the ammonia nitrogen (NH3-N) and dissolved oxygen (DO) content in the culture water of amphioxus that had burrowed into the sand, cultured using the water exchange methods described in ①-④ and Example 3, before daily feeding. Each group was tested three times over three consecutive days, and the average value was calculated. The survival rate of adult amphioxus was also statistically analyzed, and the results are shown in Table 3.

[0050] Table 3. The impact of water exchange methods on the water quality of amphioxus farming after sand burrowing.

[0051] Table 3 shows that different water exchange methods have a certain impact on the ammonia nitrogen and dissolved oxygen content in the aquaculture water for amphioxus. When the water is changed only once a day, the ammonia nitrogen content is significantly higher than that of water changed twice a day, while the dissolved oxygen content is significantly lower. During water exchange, the rotation speed of the axle has little impact on water quality, but it does have a certain impact on the survival rate of adult amphioxus. When the axle speed is low (5 r / min), pollutants in the sand layer are not completely removed, which has a certain impact on the survival rate of adult amphioxus. When the axle speed is high (15 r / min), pollutants in the sand layer are removed more thoroughly, but excessively high speed causes a greater stress response in amphioxus, which also reduces the survival rate to some extent. Therefore, the water exchange method described in Example 3 is the most suitable.

[0052] The above embodiments merely illustrate the basic principles and characteristics of the present invention. The present invention is not limited to the above embodiments. Various changes and modifications can be made to the present invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for cultivating amphioxus, characterized in that, The process is carried out using an upflow system for artificial breeding of amphioxus, which includes a breeding pond (1) and an inlet pipe (2). The support (11) is located at the bottom of the cultivation tank (1). A hollow brick layer (12) is set on the support (11). A layer of silk cloth (13) is covered on the hollow brick layer (12). A sand layer (14) is laid on top of the silk cloth layer (13). The water inlet pipe (2) is equipped with an upper water inlet (21) and a lower water inlet (22) that are connected to the upper and lower parts of the cultivation tank (1) respectively. The cultivation tank (1) is also equipped with an upper water outlet (31) and a lower water outlet (32). It also includes a rotating shaft (4), which is rotatably installed in the cultivation tank (1). The rotating shaft (4) is buried in the sand layer (14). The rotating shaft (4) is driven to rotate by a motor (5) installed outside the cultivation tank (1). Several protrusions (41) are provided on the rotating shaft (4). The specific steps for cultivating amphioxus using an upflow / downflow system for artificial breeding are as follows: S1. In the cultivation tank of the riser-faller system, a layer of hollow bricks, a layer of silk cloth, and a layer of sand are laid in sequence; and filtered fresh seawater is injected. S2. The hatched amphioxus larvae are placed in the cultivation pond of the riser system for cultivation at a stocking density of 100-160 fish / L; golden algae are fed twice a day; the water is changed once a day. The specific operation is as follows: between the two feedings, the upper inlet (21) and the lower outlet (32) are opened, the water exchange speed is 1-3 cm / s, the inlet speed is the same as the outlet speed, and the rotating shaft (4) is started to rotate at a speed of 5-10 r / min; the water exchange operation is carried out for 10-15 minutes. S3. When it is observed that the larvae of the amphioxus begin to burrow into the sand, change the water once a day. The specific operation is as follows: between two feedings, open the upper water inlet (21) and the lower water outlet (32), change the water at a speed of 1-3 cm / s, with the water inlet speed being the same as the water outlet speed, and perform the water change operation for 10-20 minutes without starting the rotating shaft (4). S4. After the amphioxus has finished burrowing in the sand, feed it a mixture of golden algae and hornwort twice a day; change the water twice a day. The specific operation is as follows: the first water change is between the two feedings, open the lower inlet (22) and the upper outlet (31), the water change speed is 2-5cm / s, the inlet speed is the same as the outlet speed, and start the rotating shaft (4) to rotate, the rotation speed of the rotating shaft (4) is 5-15r / min; perform the water change operation for 10-30min; the second water change is 6 hours after the second feeding, open the upper inlet (21) and the lower outlet (32), the water change speed is 1-3cm / s, the inlet speed is the same as the outlet speed, and perform the water change operation for 10-30min.

2. The method for cultivating amphioxus according to claim 1, characterized in that, In step S1, the stocking density of Chinese chanyu larvae is 140 fish / L.

3. The method for cultivating amphioxus according to claim 1, characterized in that, The water exchange rate in step S3 is 2 cm / s, and the water exchange time is 15 min.

4. The method for cultivating amphioxus according to claim 1, characterized in that, In step S4, the first water change rate is 4 cm / s and the water change time is 15 min; the second water change rate is 2 cm / s and the water change time is 30 min.

5. The method for cultivating amphioxus according to any one of claims 1-4, characterized in that, In step S4, the rotational speed of the shaft is 10 r / min.

6. The method for cultivating amphioxus according to claim 1, characterized in that, The sealing block (6) is fitted onto the rotating shaft (4) and fixed to the cultivation tank (1) by bolts. The first sealing strip (61) is set between the sealing block (6) and the cultivation tank (1), and the second sealing strip (62) is set between the sealing block (6) and the rotating shaft (4). The bearing seat (42) is installed on the cultivation tank (1), and the motor (5) is installed on the bearing seat (42).

7. The method for cultivating amphioxus according to claim 6, characterized in that, An annular groove is provided on the end face of the sealing block (6) for installing the first sealing strip (61).

8. The method for cultivating amphioxus according to claim 6, characterized in that, A V-shaped groove is provided in the inner hole of the sealing block (6) for installing the second sealing strip (62).

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