Carassius auratus low oxygen stress experiment device

Abstract

This invention relates to the field of fishery experiments and discloses a hypoxia stress experimental device for Chuzhou crucian carp. The device includes a culture structure for aquaculture and an oxygen control mechanism for oxygen control. The culture structure includes an outermost culture tank with multiple disturbance rods connected to one side. A partition plate is fixedly connected inside the culture tank, and multiple separation pipes are connected to the bottom of the partition plate. A slow-flow pipe connected to the oxygen control mechanism is connected to the bottom of the culture tank. A circulation sleeve connected to the culture tank is provided on the partition plate. This invention can continuously monitor water quality filtration and, in conjunction with aeration and oxygen supply functions, ensure stable oxygen content in the water. It can also control multiple variables to maintain stability, ensuring the accuracy of the final experimental data and providing better data support for subsequent aquaculture.

Description

Technical Field

[0001] This invention relates to the field of fishery experiments, and more particularly to an experimental apparatus for hypoxia stress in Chuzhou crucian carp. Background Technology

[0002] Chuzhou crucian carp is a specialty aquatic product of Chuzhou City, Anhui Province, renowned for its delicious meat and rich nutrition. Located between the Yangtze and Huai Rivers, Chuzhou boasts abundant water resources, ideal for crucian carp growth. Chuzhou crucian carp are typically medium-sized with tender flesh, suitable for various cooking methods such as steaming, braising, and stewing. Hypoxic stress experiments in fish are an important tool for studying the physiological, biochemical, and molecular response mechanisms of fish under hypoxic (anoxic) environments. They are commonly used to assess the hypoxic tolerance of fish, screen hypoxic-tolerant species, or explore their adaptation mechanisms.

[0003] Existing experimental methods generally rely on the oxygen consumption of crucian carp through respiration to reach the required oxygen level in the water before supplementing the water with oxygen to balance the oxygen concentration. However, this method has problems such as complex operation and low control precision. Multiple variables cannot be well controlled, which affects the experimental data and causes deviations in the final experimental results. Summary of the Invention

[0004] To address the technical problems in the experiment, this invention provides an experimental apparatus for hypoxia stress in Chuzhou crucian carp.

[0005] The present invention is achieved by the following technical solution: a hypoxia stress experimental device for Chuzhou crucian carp, including a breeding structure for aquaculture and an oxygen control structure for oxygen control; As a further improvement to the above scheme, the breeding structure includes an outermost breeding box, with multiple disturbance rods connected to one side of the breeding box, a partition plate fixedly connected inside the breeding box, multiple separation pipes connected to the bottom of the partition plate, a slow-flow pipe connected to the oxygen control mechanism at the bottom of the breeding box, and a circulation sleeve connected to the breeding box on the partition plate. As a further improvement to the above scheme, the oxygen control mechanism includes a circulation box connected to a slow-flow pipe. Inside the circulation box, there is a filter box one connected to the slow-flow pipe. A circulation pump is connected to one side of the filter box one. The output end of the circulation pump is connected to a filter box two. A transparent box located outside the circulation box is connected to one side of the filter box two. An aeration box is connected to one side of the transparent box. One side of the aeration box is connected in sequence to pipe one, detector one, pipe two, detector two, pipe three, and a buffer box. Pipe five is connected between detector one and filter box two. A return pipe connected to the circulation sleeve is connected to one side of the buffer box.

[0006] As a further improvement to the above scheme, a sealed box is set inside the transparent box, and a motor is fixedly connected inside the sealed box. The output end of the motor is driven by a gearbox, and the output end of the gearbox is driven by a drain pipe. The fixed point of the drain pipe is connected to a rotating ring located inside the aeration box. Multiple flexible tubes are fixedly connected around the periphery of the rotating ring. An oxygen pipe extending to the outside of the aeration box is fixedly and rotatably connected to the center of the rotating ring. A drain pipe extending into the transparent box is fixedly connected to the bottom of the aeration box.

[0007] As a further improvement to the above scheme, multiple aeration holes are provided on both the rotating ring and the flexible tube, and multiple exhaust pipes are fixedly connected to the aeration box.

[0008] As a further improvement to the above scheme, a replenishment box is fixedly connected inside the circulation box, and a pipe four connected to the filter box is fixedly connected to one side of the replenishment box. A functional pipe extending to the outside of the circulation box is connected to one side of the detector.

[0009] As a further improvement to the above scheme, one side of filter box one and filter box two are snapped into the circulation box, and one side of filter box one and filter box two are fixedly connected with a sealing door that fits into the circulation box. The circulation box is provided with a cleaning hole for filter box one and filter box two to pass through.

[0010] As a further improvement to the above solution, a control sleeve is fixedly connected inside the breeding box, and multiple illumination lamps are fixedly connected to the control sleeve. One side of the control sleeve extends to the outside of the breeding box, and multiple air holes are provided on the control sleeve. A pressure control tube is connected to the end of the control sleeve that extends to the outside of the breeding box, and a pressure control valve is connected to the pressure control tube.

[0011] As a further improvement to the above solution, a flexible sleeve is connected to one side of the breeding box, and a gripping hole connected to the flexible sleeve is provided on the breeding box. A transparent plate is provided on one side of the breeding box, and a sunshade curtain connected to the breeding box is provided on one side of the transparent plate.

[0012] As a further improvement to the above solution, a guide plate is installed at the bottom of the breeding box, and a monitoring instrument connected to the breeding box is installed on one side of the guide plate.

[0013] As a further improvement to the above scheme, a support plate is installed at the bottom of the breeding facility and the oxygen control facility. A vertical plate is connected to one side of the support plate, and a horizontal plate that is fixed to the ground is connected to the bottom of the vertical plate. An air supply pipe is connected to one side of the vertical plate, and multiple connecting pipes are connected to one side of the vertical plate. The connecting pipes are connected to the oxygen pipe through pipelines.

[0014] As a further improvement to the above solution, filter cotton is installed inside the vertical plate, and a water supply pipe is connected to one side of the horizontal plate. One end of the water supply pipe is connected to a flexible hose, and the other end of the flexible hose is connected to a supplementary pipe that connects to the breeding box.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. Through the cooperation of multiple components, continuous water quality filtration and monitoring can be carried out. At the same time, with the aeration and oxygen supply function, the oxygen content of the water can be kept stable. It can also control multiple variables to maintain stability, ensuring the accuracy of the final experimental data and providing better data support for subsequent aquaculture.

[0016] 2. By working with multiple oxygen control and aquaculture facilities, a modular testing system can be formed, enabling large-scale hypoxia stress experiments to be conducted simultaneously, reducing experimental time. Different operations can be performed during the experiment to meet the needs of the experiment and facilitate its execution. Attached Figure Description

[0017] Figure 1 This is a front view structural diagram of the present invention; Figure 2 This is a rear view structural diagram of the present invention; Figure 3 This is a schematic diagram of the main sectional view of the oxygen control mechanism; Figure 4 This is a partial rear view of the oxygen control mechanism. Figure 5 This is a partial front view of the oxygen control mechanism. Figure 6 A front view of the aquaculture facility; Figure 7 This is a schematic diagram of the main sectional view of the aquaculture facility; Figure 8 This is a partial front view of the aquaculture facility. Figure 9 This is a schematic diagram of the main sectional view of the aquaculture facility; Figure 10 This is a schematic diagram of the experimental setup for hypoxia stress in Chuzhou crucian carp.

[0018] Explanation of key symbols: 01. Horizontal plate; 02. Vertical plate; 03. Connecting pipe; 04. Oxygen control mechanism; 05. Breeding mechanism; 11. Breeding box; 12. Flexible sleeve; 14. Oxygen pipe; 15. Exhaust pipe; 16. Circulation box; 17. Return pipe; 18. Slow flow pipe; 20. Supplement pipe; 21. Functional pipe; 22. Disturbance rod; 23. Circulation sleeve; 24. Separation pipe; 25. Guide plate; 26. Divider plate; 27. Control sleeve; 28. 30. Irradiation lamp; 31. Filter box one; 32. Filter box two; 33. Transparent box; 34. Pipe one; 35. Aeration box; 36. Detector one; 37. Pipe two; 38. Detector two; 39. Replenishment box; 40. Buffer box; 41. Pipe three; 43. Pipe four; 44. Circulation pump; 45. Pipe five; 50. Rotating ring; 51. Flexible tube; 52. Motor; 53. Sealed box; 54. Gearbox; 55. Drainage tube. Detailed Implementation

[0019] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments.

[0020] Example 1

[0021] Please combine Figures 1-9 , The Chuzhou crucian carp hypoxia stress experimental device includes an aquaculture unit 05 for aquaculture and an oxygen control unit 04 for oxygen control. By separating the oxygen control unit 04 from the aquaculture unit 05, the impact of noise and other factors during the operation of the device on the fish population in the aquaculture unit 05 is reduced.

[0022] The aquaculture apparatus 05 includes an outermost aquaculture tank 11. Multiple disturbance rods 22 are connected to one side of the aquaculture tank 11. A partition plate 26 is fixedly connected inside the aquaculture tank 11. Multiple separation pipes 24 are connected to the bottom of the partition plate 26. A slow-flow pipe 18 connected to the oxygen control mechanism 04 is connected to the bottom of the aquaculture tank 11. A circulation sleeve 23 connected to the aquaculture tank 11 is installed on the partition plate 26. The aquaculture tank 11 is selected based on the size of the crucian carp, while ensuring the density of the fish population inside and maintaining stable experimental parameters. The disturbance rods 22 can automatically extend to disturb the fish population, forcing them to swim and conduct different experiments. The partition plate 26 separates the fish, and the excrement or impurities above are discharged to the bottom through the separation pipes 24 for collection. Then, the waste is discharged through the slow-flow pipe 18. Under the control of external water pressure, the circulation sleeve 23 allows water to overflow at different flow rates for replenishment.

[0023] The oxygen control mechanism 04 includes a circulation box 16 connected to a slow-flow pipe 18. A filter box 30 connected to the slow-flow pipe 18 is installed inside the circulation box 16. A circulation pump 44 is connected to one side of the filter box 30. A filter box 31 is connected to the output end of the circulation pump 44. A transparent box 32 located outside the circulation box 16 is connected to one side of the filter box 31. An aeration box 34 is connected to one side of the transparent box 32. A pipe 33, a detector 36, a second pipe 37, a second detector 38, a third pipe 41, and a buffer box 40 are sequentially connected to one side of the aeration box 34. A fifth pipe 45 connects the detector 36 and the filter box 31. A return pipe 17 connected to the circulation sleeve 23 is connected to the side. The filter box 30 performs initial filtration, and the circulation pump 44 pumps the water. After passing through the filter box 31 for further filtration, the water enters the transparent box 32 and then the aeration box 34. The aeration box 34 is filled with oxygen to supplement oxygen. After monitoring by the detector 36, if the oxygen content is low, some water returns to the filter box 31 through the pipe 45 for another circulation. Then, the water enters the detector 38 through the pipe 37 for a second detection. Finally, it passes through the buffer box 40 and the return pipe 17 and re-enters the circulation sleeve 23 for circulation.

[0024] A sealed box 53 is installed inside the transparent box 32. A motor 52 is fixedly connected inside the sealed box 53. The output end of the motor 52 is driven by a gearbox 54. The output end of the gearbox 54 is driven by a drain pipe 55. The positioning point of the drain pipe 55 is connected to a rotating ring 50 located inside the aeration box 34. Multiple flexible tubes 51 are fixedly connected around the rotating ring 50. An oxygen pipe 14 extending to the outside of the aeration box 34 is fixedly rotatably connected to the center of the rotating ring 50. The bottom of the aeration box 34 is fixedly connected to the drain pipe 55 extending into the transparent box 32. Through the power output of the motor 52 and the speed change of the gearbox 54, the drain pipe 55 is driven to rotate, which in turn drives the rotating ring 50 and the flexible tubes 51 to rotate. The oxygen pipe 14 connects to the external oxygen supply to aerate the water and increase the oxygen content. The water in the transparent box 32 enters the aeration box 34 through the drain pipe 55 and then is discharged through pipe 33.

[0025] Both the rotating ring 50 and the flexible tube 51 are provided with multiple aeration holes, and multiple exhaust pipes 15 are fixedly connected to the aeration box 34. The aeration holes ensure the passage of gas, and the exhaust pipes 15 discharge excess gas to ensure the water pressure in the aeration box 34.

[0026] A replenishment tank 39 is fixedly connected inside the circulation tank 16. A pipe 43 connected to the filter box 30 is fixedly connected to one side of the replenishment tank 39. A functional pipe 21 extending to the outside of the circulation tank 16 is connected to one side of the detector 36. Flocculant and disinfectant are provided inside the replenishment tank 39 to flocculate and disinfect the water.

[0027] One side of filter box 30 and filter box 31 are snapped into circulation box 16. One side of filter box 30 and filter box 31 is fixedly connected to a sealing door that fits into circulation box 16. Circulation box 16 is provided with a cleaning hole for filter box 30 and filter box 31 to pass through. Filter box 30 and filter box 31 can be easily disassembled and installed through the sealing door, facilitating internal maintenance and cleaning. Filter box 30 and filter box 31 are connected to other devices through flexible hoses.

[0028] A control sleeve 27 is fixedly connected inside the breeding box 11. Multiple illumination lamps 28 are fixedly connected to the control sleeve 27. One side of the control sleeve 27 extends to the outside of the breeding box 11. Multiple air holes are provided on the control sleeve 27. A pressure control tube is connected to the end of the control sleeve 27 that extends to the outside of the breeding box 11. A pressure control valve is connected to the pressure control tube. The air pressure inside the breeding box 11 can be adjusted through the pressure control tube to ensure the stability of the parameters inside the breeding box 11. The illumination lamps 28 provide supplementary lighting. At the same time, the control sleeve 27 is also connected to a camera for real-time monitoring of the status.

[0029] A flexible sleeve 12 is connected to one side of the breeding box 11. The breeding box 11 is provided with a gripping hole that communicates with the flexible sleeve 12. A transparent plate is provided on one side of the breeding box 11. A sunshade curtain connected to the breeding box 11 is provided on one side of the transparent plate. The flexible sleeve 12 is made of flexible material. Experimenters can reach into the breeding box 11 through the flexible sleeve 12 to perform certain operations.

[0030] A guide plate 25 is provided at the bottom of the breeding box 11. A monitoring instrument connected to the breeding box 11 is provided on one side of the guide plate 25. The guide plate 25 guides impurities, and the monitoring instrument detects water parameters.

[0031] The implementation principle of this application embodiment is as follows: At the start of operation, water is injected into the breeding tank 11, and then pumped using the circulation pump 44 in the oxygen control mechanism 04. The water flows through the slow-flow pipe 18, filter box one 30, and filter box two 31 before entering the transparent box 32. Filter box one 30 and transparent box 32 perform preliminary purification of the water. After passing through transparent box 32, drainage pipe 55, aeration box 34, pipe one 33, detector one 36, pipe two 37, detector two 38, and pipe three 41, the water enters the buffer tank 40. Simultaneously, oxygen is introduced through oxygen pipe 14 and... Efficient aeration is achieved through the aeration holes of the rotating ring 50 and flexible pipe 51. Detector 1 36 and Detector 2 38 provide real-time data feedback. If dissolved oxygen is insufficient, some water is returned to filter box 2 31 through pipe 5 45 for secondary oxygenation. Finally, the regulated water is transported to the circulation sleeve 23 through buffer box 40 and return pipe 17 to form a closed loop. When the entire system is stable, the corresponding number of crucian carp are placed in the breeding box 11. The separator 26 and the bottom separation pipe 24 work together to separate the waste, and at the same time, the waste is circulated and cleaned through the slow flow pipe 18.

[0032] The adjustable disturbance rod 22 can apply controllable disturbance to the Chuzhou crucian carp population to simulate different swimming intensities. The control sleeve 27 and the illumination lamp 28 maintain the stability of the cabin environment and ensure the precise control of oxygen delivery and water quality parameters. The overall device reduces external interference through physical separation and automated monitoring, ensuring the efficient collection and repeatability of fish behavior, physiological response and environmental data in the hypoxia stress experiment.

[0033] Example 2

[0034] Combination Figures 1-10 Based on Example 1, this embodiment further improves upon the following: a support plate is installed at the bottom of the aquaculture mechanism 05 and the oxygen control mechanism 04. A vertical plate 02 is connected to one side of the support plate, and a horizontal plate 01 fixedly connected to the ground is connected to the bottom of the vertical plate 02. An air supply pipe is connected to one side of the vertical plate 02, and multiple connecting pipes 03 are connected to one side of the vertical plate 02. The connecting pipes 03 are connected to the oxygen pipe 14 through pipes. With the support of the horizontal plate 01 and the vertical plate 02, multiple oxygen control mechanisms 04 and aquaculture mechanisms 05 can be supported in conjunction with the support plate, thereby enabling multiple experiments to be carried out simultaneously and increasing the overall experimental efficiency.

[0035] The vertical plate 02 is equipped with filter cotton, and a water supply pipe is connected to one side of the horizontal plate 01. One end of the water supply pipe is connected to a flexible hose, and the other end of the flexible hose is connected to a replenishment pipe 20 connected to the breeding box 11. The filter cotton filters out airborne particles, and the water supply pipe supplies water to the breeding box 11 to replenish any water that may be lost in the breeding box 11.

[0036] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. An experimental apparatus for hypoxia stress in Chuzhou crucian carp, characterized in that, This includes aquaculture facilities and oxygen control facilities. The breeding mechanism includes an outermost breeding box, with multiple disturbance rods connected to one side of the breeding box. A partition plate is fixedly connected inside the breeding box, with multiple separation pipes connected to the bottom of the partition plate. A slow-flow pipe connected to the oxygen control mechanism is connected to the bottom of the breeding box, and a circulation sleeve connected to the breeding box is provided on the partition plate. The oxygen control mechanism includes a circulation box connected to a slow-flow pipe. Inside the circulation box is a filter box one connected to the slow-flow pipe. A circulation pump is connected to one side of the filter box one. The output end of the circulation pump is connected to a filter box two. A transparent box located outside the circulation box is connected to one side of the filter box two. An aeration box is connected to one side of the transparent box. A pipe one, a detector one, a pipe two, a detector two, a pipe three, and a buffer box are connected in sequence to one side of the aeration box. A pipe five is connected between the detector one and the filter box two. A return pipe connected to the circulation sleeve is connected to one side of the buffer box. The transparent box contains a sealed box, and a motor is fixedly connected inside the sealed box. The output end of the motor is driven by a gearbox, and the output end of the gearbox is driven by a drain pipe. One end of the drain pipe is fixedly connected to a rotating ring located inside the aeration box. Multiple flexible tubes are fixedly connected around the periphery of the rotating ring, and an oxygen tube extending to the outside of the aeration box is rotatably connected to the center of the rotating ring. The bottom of the breeding facility and the oxygen control facility is equipped with a support plate. A vertical plate is connected to one side of the support plate, and a horizontal plate is fixedly connected to the ground at the bottom of the vertical plate. An air supply pipe is connected to one side of the vertical plate, and multiple connecting pipes are connected to one side of the vertical plate. The connecting pipes are connected to the oxygen pipe through pipelines. Both the rotating ring and the flexible tube are provided with multiple aeration holes, and multiple exhaust pipes are fixedly connected to the aeration box; the rotation of the diversion pipe is driven by the power output of the motor and the speed change of the gearbox; the water in the transparent box enters the aeration box through the diversion pipe, and then is discharged through pipe one.

2. The hypoxia stress experimental apparatus for Chuzhou crucian carp as described in claim 1, characterized in that, A replenishment box is fixedly connected inside the circulation box. A pipe four connected to the filter box is fixedly connected to one side of the replenishment box. A functional pipe extending to the outside of the circulation box is connected to one side of the detector.

3. The hypoxia stress experimental apparatus for Chuzhou crucian carp as described in claim 1, characterized in that, One side of each of the filter boxes is snapped into the circulation box. One side of each of the filter boxes is fixedly connected to a sealing door that fits into the circulation box. The circulation box is provided with a cleaning hole for the filter boxes to pass through.

4. The hypoxia stress experimental apparatus for Chuzhou crucian carp as described in claim 1, characterized in that, A control sleeve is fixedly connected inside the breeding box, and multiple illumination lamps are fixedly connected to the control sleeve. One side of the control sleeve extends to the outside of the breeding box, and multiple air holes are provided on the control sleeve. A pressure control tube is connected to the end of the control sleeve that extends to the outside of the breeding box, and a pressure control valve is connected to the pressure control tube.

5. The hypoxia stress experimental apparatus for Chuzhou crucian carp as described in claim 1, characterized in that, A flexible sleeve is connected to one side of the breeding box, and a gripping hole communicating with the flexible sleeve is provided on the breeding box. A transparent plate is provided on one side of the breeding box, and a sunshade curtain connected to the breeding box is provided on one side of the transparent plate.

6. The hypoxia stress experimental apparatus for Chuzhou crucian carp as described in claim 1, characterized in that, The bottom of the breeding box is equipped with a guide plate, and a monitoring instrument connected to the breeding box is installed on one side of the guide plate.

7. The hypoxia stress experimental apparatus for Chuzhou crucian carp as described in claim 1, characterized in that, The vertical plate is equipped with filter cotton, and a water supply pipe is connected to one side of the horizontal plate. One end of the water supply pipe is connected to a flexible hose, and the other end of the flexible hose is connected to a supplementary pipe that connects to the breeding box.

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