Human rotavirus strain RV009 and methods for isolating, culturing and identifying same

CN120173890BActive Publication Date: 2026-07-03JIANGSU LEVIESTER BIOTECHNOLOGY CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU LEVIESTER BIOTECHNOLOGY CO LTD
Filing Date
2025-02-27
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Currently, it is not possible to directly purchase popular G9 rotavirus strains, and existing technologies make it difficult to effectively isolate and culture G9 rotavirus for vaccine research, thus limiting vaccine development.

Method used

This study provides methods for the isolation, culture, and identification of human G9 rotavirus strain RV009. G9 strains were screened using techniques such as colloidal gold detection, RNA-PAGE, and RT-PCR. The strains were then adapted and purified in MA104 and Vero cells. Culture conditions were optimized to improve viral replication capacity and genetic stability.

Benefits of technology

The G9 rotavirus strain RV009, which exhibits good genetic stability and strong replication ability, was successfully isolated. It is suitable for developing rotavirus vaccines. The isolation and culture conditions were optimized, which improved viral infectivity and yield, and supports vaccine research and clinical application.

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Abstract

This invention discloses a human rotavirus strain and its isolation, culture, and identification methods. The human rotavirus strain described in this invention is rotavirus RV009, which was deposited with the China General Microbiological Culture Collection Center (CGMCC) on July 22, 2024, with accession number CGMCC No. 45999. The isolation, culture, and identification methods for human rotavirus strain RV009 provided by this invention facilitate rapid virus amplification, shorten the production cycle, and obtain high-yield viruses at a lower cost, which is beneficial for vaccine production, virological research, and the development of diagnostic reagents.
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