一种超灵敏生物传感器及其制备方法与应用
By combining two-dimensional materials, DNA origami, and quantum dots, a biosensor utilizing CRISPR/Cas technology has solved the problem of low sensitivity in existing biosensors, achieving high-sensitivity detection at the aM level.
CN120210332BActive Publication Date: 2026-07-17SHENZHEN UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHENZHEN UNIV
- Filing Date
- 2025-03-27
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing biosensors are not sensitive enough to detect biomarkers in extremely low concentrations.
Method used
By combining two-dimensional materials, DNA origami, and quantum dots, and using CRISPR/Cas technology, the distance between quantum dots and two-dimensional materials is precisely controlled through the second harmonic effect and DNA origami. The CRISPR/Cas technology is then used to identify target nucleic acids and generate detection signals by releasing quantum dots through shearing.
Benefits of technology
It significantly improves the sensitivity of biosensors to the aM level, enabling the identification of low-abundance nucleic acid targets in complex biological samples without sample preparation or amplification.
✦ Generated by Eureka AI based on patent content.
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Figure CN120210332B_ABST
Abstract
本发明涉及生物传感技术领域，公开了一种超灵敏生物传感器及其制备方法与应用。所述超灵敏生物传感器包括：基底、负载在所述基底上的二维材料、负载在所述二维材料上的DNA折纸和修饰在所述DNA折纸上的量子点；其中，所述DNA折纸中含有暴露的单链DNA片段，所述单链DNA片段可被激活的Cas蛋白切割；所述量子点与所述单链DNA片段的末端相连。该超灵敏生物传感器性能优越，与常规的表面等离子体共振和DNA折纸结合的纯光学生物传感器相比，灵敏度提高了四个数量级，可达aM水平。
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