Method for observing the process of fecal bait dissipation

Abstract

The application provides an experimental method for observing the dissipation process of fecal bait, which comprises the following steps: obtaining the required sediments and fecal bait, detecting indexes of the sediments and the fecal bait to obtain first parameters; placing the fecal bait on the sediments laid in an experimental device; configuring a water flow passing through the sediments and the fecal bait in the experimental device, and adjusting environmental characteristics in the experimental device to simulate the environment of a target area; regularly detecting indexes of the water flow in the experimental device, and recording the dissipation of the fecal bait; after the experiment is terminated, detecting indexes of the sediments and the fecal bait in the experimental device to obtain second parameters; comparing the second parameters with the first parameters to obtain the dissipation of the sediments and the fecal bait under the simulated environment in the experimental device; and solving the problems of unrepeatable environmental conditions, inconvenient personnel observation and difficult sampling in the observation of the dissipation experiment of the fecal bait.

Description

Technical Field

[0001] This application relates to the technical field of environmental science and engineering experiments, and in particular to an experimental method for observing the dissipation process of fecal bait. Background Technology

[0002] Experiments observing the dissipation process of residual feed and fish feces play a crucial role in aquaculture management. These experiments not only reveal feed utilization efficiency and fish health but also assess the impact of the aquaculture process on water quality. Specifically, undigested feed residues in the water, along with fish feces, affect water quality, indirectly influencing fish growth and survival rates. Therefore, in-depth research into the dissipation mechanisms of these substances in water helps optimize feeding strategies, improve water quality management, and ultimately enhance aquaculture efficiency.

[0003] Currently, research on the dissipation processes of residual feed and fish feces largely relies on outdoor field experiments. These experiments are typically conducted directly in aquaculture ponds or open water areas to simulate actual conditions under natural conditions. The advantage of this approach is that it provides relatively realistic environmental data, making the research findings more relevant to practical applications. For example, researchers can periodically sample and analyze data to monitor changes in the concentration of feed residues and fecal particles in the water over different time periods, obtaining information such as their dissipation rates and pathways under natural conditions.

[0004] However, existing outdoor experimental methods have significant technical limitations. Because these experiments are conducted in natural environments, it is difficult to precisely control key variables such as temperature, light intensity, and water flow rate. This results in inconsistent environmental conditions for each experiment, severely impacting the repeatability and reliability of the results and limiting in-depth research into the mechanisms of fecal feed dissipation. More importantly, conducting experiments directly in aquaculture ponds or open water areas hinders the safe and efficient sampling process and increases the risk of sample contamination, complicating subsequent testing and analysis. These issues indicate that existing experimental methods urgently need improvement to provide more accurate and reliable data support for scientific research and technological development. Summary of the Invention

[0005] This application provides an experimental method for observing the dissipation process of fecal bait, in order to solve the technical problems of unreplicable environmental conditions, inconvenience for personnel observation, and difficulty in sampling due to site limitations in fecal bait dissipation experiments. The technical solution is as follows:

[0006] This application provides an experimental method for observing the dissipation process of fecal bait, including:

[0007] Obtain the required sediment and fecal bait, and test various indicators of the sediment and fecal bait to obtain the first parameter;

[0008] The fecal bait was placed on the sediment laid in the experimental equipment;

[0009] The experimental setup was configured with water flow containing sediment and fecal bait, and the environmental characteristics of the setup were adjusted to simulate the environment of the target area.

[0010] Regularly test various parameters of the water flow in the experimental equipment and record the dissipation of feces and bait;

[0011] After the experiment was terminated, various indicators of the sediment and fecal bait in the experimental equipment were measured to obtain the second parameter;

[0012] By comparing the second parameter with the first parameter, the dissipation of sediment and fecal bait under the simulated environment in the experimental equipment can be obtained.

[0013] In one embodiment, the indicators to be tested for sediment and fecal bait include: total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), crude protein, ash content, moisture content, carbon isotopes, nitrogen isotopes, and heavy metals.

[0014] In one embodiment, the method of configuring water flow carrying via sediments and fecal bait in an experimental setup, and adjusting environmental characteristics within the experimental setup to simulate the environment of a target area, further includes:

[0015] Adjusting the temperature of the water flow in the experimental equipment;

[0016] Adjust the aeration rate of the water flow in the experimental equipment;

[0017] Adjust the water flow, sediment, and light conditions of the feces bait in the experimental equipment.

[0018] In one embodiment, the method for adjusting the temperature of the water flow in the experimental apparatus further includes:

[0019] Adjust the water temperature in the experimental equipment to 16℃~36℃.

[0020] In one embodiment, the method for adjusting the aeration rate of water flow in the experimental equipment further includes:

[0021] The aeration rate of the water flow in the experimental equipment was adjusted to 0%–100%.

[0022] In one embodiment, the method for adjusting the light conditions of water flow, sediment, and fecal bait in the experimental apparatus further includes:

[0023] The experimental equipment was shielded from light to ensure that the water flow, sediment, and fecal bait within the equipment were kept in a dark environment; or,

[0024] The experimental equipment was made transparent, and the light source was shone on the water flow, sediment, and feces in the experimental equipment.

[0025] In one embodiment, the method for periodically monitoring various parameters of the water flow in the experimental equipment and recording the dissipation of fecal bait further includes:

[0026] The primary water quality indicator in the water flow is tested regularly, with an interval of 24 hours between each test.

[0027] The primary water quality indicators in the water flow are water temperature, water color, pH value, conductivity, and dissolved oxygen content.

[0028] In one embodiment, the method for periodically monitoring various parameters of the water flow in the experimental equipment and recording the dissipation of fecal bait further includes:

[0029] The second water quality indicator in the water flow is tested regularly, with an interval of 24 hours to 600 hours between each test.

[0030] The second water quality indicators in the flow were total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH4-N), nitrite nitrogen (NO2-N), and nitrate nitrogen (NO3-N). - (N), reactive phosphate, chemical oxygen demand and heavy metals.

[0031] In one embodiment, the method for periodically monitoring various parameters of the water flow in the experimental equipment and recording the dissipation of fecal bait further includes:

[0032] The first and second water quality indicators of the water flow are compared with the various indicators of tap water to obtain the water quality differences between the water flow and tap water.

[0033] In one embodiment, the method for obtaining the second parameter by detecting various indicators of sediment and fecal bait in the experimental equipment after the experiment is terminated further includes:

[0034] This experiment will terminate when one of the following conditions is met:

[0035] Visually, the fecal bait in the experimental equipment has completely dissipated;

[0036] The experiment to observe the dissipation process of fecal bait lasted for three months;

[0037] Among the methods for regularly testing various indicators of water flow in experimental equipment and recording the dissipation of feces and bait, the test results of water flow indicators are stable.

[0038] The method of regularly testing various indicators of water flow in experimental equipment and recording the dissipation of feces and bait yields water quality test results that are no different from those of tap water.

[0039] Compared with existing technologies, the experimental method for observing the dissipation process of fecal bait proposed in the above-mentioned technical solution significantly improves the accuracy and reproducibility of research on the dissipation process of residual bait and fish feces by observing the dissipation of fecal bait in an experimental device. Firstly, by laying a sedimentary layer similar to the target area within the experimental device and placing fecal bait on top, and then simulating the water flow conditions of the target area, the experimental environment is made as close to natural conditions as possible while maintaining controllability. The experimental device allows researchers to precisely adjust environmental characteristics, such as key environmental parameters like water flow rate and velocity, aeration rate, water temperature, and light intensity, ensuring consistency of experimental conditions for each experiment, thereby greatly improving the reliability and reproducibility of experimental results. Furthermore, compared with traditional outdoor experimental methods, this application not only overcomes the influence of uncontrollable factors in the natural environment but also provides a more convenient sampling and water quality testing approach. Researchers can safely and efficiently collect samples and conduct subsequent analysis without interfering with the experimental process, which greatly facilitates the study of fecal bait dissipation mechanisms under different conditions. Since all operations are performed inside the experimental equipment, the risk of sample contamination associated with open water experiments is avoided, further ensuring the accuracy of the experimental data.

[0040] In summary, this application enables a refined study of the dissipation process of residual feed and fish feces in water bodies, providing solid data support and technical assurance for optimizing aquaculture management strategies and improving water quality. This improvement not only helps enhance aquaculture efficiency and sustainability but also provides new tools and ideas for scientific research in related fields.

[0041] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of this application will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0042] In the accompanying drawings, unless otherwise specified, the same reference numerals throughout the various drawings denote the same or similar parts or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings depict only some embodiments disclosed in this application and should not be construed as limiting the scope of this application.

[0043] Figure 1 This is a schematic diagram of the water tank in the experimental device for observing the dissipation process of fecal bait as proposed in Embodiment 2 of this application;

[0044] Figure 2 This is a schematic diagram of the structure of the rectifier board in the first embodiment of Embodiment 2 of this application;

[0045] Figure 3 This is a schematic diagram of the structure of the rectifier board in the second embodiment of this application.

[0046] Figure label:

[0047] 1. Water tank;

[0048] 11. Inlet; 12. Outlet; 1a. First chamber; 1b. Second chamber; 1c. Third chamber;

[0049] 2. Rectifier plate; 20. Hole bar; 200. Drain hole;

[0050] 3. Overflow plate;

[0051] 4. Baffle;

[0052] 5. Water supply pipeline; 51. Ball valve; 52. Flow meter;

[0053] 6. Temperature control device;

[0054] 7. Aeration device; 71. Four-hole oxygen pump; 72. Aerator;

[0055] 8. Lighting device;

[0056] 9. Sediment layer. Detailed Implementation

[0057] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this application. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0058] Example 1

[0059] An experimental method for observing the dissipation process of fecal bait is proposed in the embodiments of this application. The experimental method for observing the dissipation process of fecal bait may include the following steps:

[0060] Obtain the required sediment and fecal bait, and test various indicators of the sediment and fecal bait to obtain the first parameter;

[0061] The fecal bait was placed on the sediment laid in the experimental equipment;

[0062] The experimental setup was configured with water flow containing sediment and fecal bait, and the environmental characteristics of the setup were adjusted to simulate the environment of the target area.

[0063] Regularly test various parameters of the water flow in the experimental equipment and record the dissipation of feces and bait;

[0064] After the experiment was terminated, various indicators of the sediment and fecal bait in the experimental equipment were measured to obtain the second parameter;

[0065] By comparing the second parameter with the first parameter, the dissipation of sediment and fecal bait under the simulated environment in the experimental equipment can be obtained.

[0066] Specifically, in the technical solution adopted in this application, sand and gravel can be selected as the sediment for implementing this method. A sediment layer of 30-50 mm thickness is laid in the experimental equipment to simulate various sedimentation environments through different sediment thicknesses. Fecal bait is placed on the sediment, and the flow rate and volume of the water in the experimental equipment are controlled to simulate different water flow conditions. This allows for the adaptation of sediments with different particle sizes by adjusting the water flow conditions in the experimental equipment. It should be noted that the sediment can be sand particles with a particle size of approximately 62-1500 μm, silt particles with a particle size of approximately 4-62 μm, or clay particles with a particle size of approximately 1-4 μm. Different particle sizes of sediment require corresponding starting flow rates (water flow) to be set in the experimental equipment. Specifically, sediments of appropriate particle sizes are selected based on the sediment in the target area, and the water flow rate is set according to the sediment particle size. The range of flow rates is determined based on the final results of preliminary experimental tests. It should be explained that the target area is the actual sedimentation area where the fecal bait needs to be dispersed. Based on experimental data, taking 500 μm sediment particles as an example, when the sediment is in a state of non-consolidation, the sediment will be washed away by the water flow when the flow velocity is greater than 18 cm·s⁻¹. Therefore, when implementing the experimental method of this application, it is recommended to set the water flow velocity to below 18 cm·s⁻¹ for sediment particles of 500 μm. It should be noted that in this experimental method, water should be prevented from freely falling onto the sediment laid in the experimental equipment due to gravity; furthermore, turbulent kinetic energy generated by the water flow through other external forces should be avoided to prevent the sediment from being washed away by the water flow.

[0067] In some embodiments, the dissipation of fecal bait can be recorded by visual inspection or video recording of the dissipation of fecal bait in the experimental equipment, and a written description can be attached after the visual inspection or video recording.

[0068] Furthermore, in some embodiments, the indicators to be tested for sediments and fecal bait include: total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), crude protein, ash content, moisture content, carbon isotopes, nitrogen isotopes, and heavy metals.

[0069] Specifically, in the technical solution adopted in this application, various indicators of sediment and fecal bait are tested according to water quality and sediment testing regulations and related requirements. A first indicator test is performed before the sediment and fecal bait are placed in the experimental equipment to obtain the first parameter. A second indicator test is performed after the experiment is completed to obtain the second parameter. It should be noted that after the experiment is completed, there may be situations where sediment and fecal bait cannot be distinguished. In such cases, appropriate measures can be taken in the second indicator test. For example, the outermost sediment can be tested for fecal bait, and the obtained indicator parameters can be used as the fecal bait indicator parameters; the bottom sediment can be tested for sediment, and the obtained indicator parameters can be used as the sediment indicator parameters. Thus, this method allows for successful second indicator testing to obtain the second parameter after the experiment is completed. In this embodiment, the heavy metals in the sediment and fecal bait indicator tests include, but are not limited to, copper, lead, zinc, cadmium, arsenic, total mercury, total chromium, nickel, and total vanadium.

[0070] In this application, the various indicators of sediment and fecal bait can be tested in the following manner:

[0071] Total organic carbon (TOC) can be detected by Shimadzu (TOC-LCPHSSM-5000A), and the analytical method after detection can refer to GB / T30740-2014 "Determination of Total Organic Carbon in Marine Sediments by Non-dispersive Infrared Absorption Method", where the method detection limit is 0.03×10-2.

[0072] Total nitrogen (TN) can be detected using a fully automated Kjeldahl nitrogen analyzer, and the analytical method after detection can refer to the Kjeldahl titration method in "Marine Monitoring Specifications Part 5: Sediment Analysis" (GB17378.5-2007 Appendix D).

[0073] Total phosphorus (TP) can be detected by a UV-Vis spectrophotometer (Shanghai Yuanxi X-8), and the analytical method after detection can refer to the spectrophotometric method "Marine Monitoring Specification Part 5: Sediment Analysis" (GB17378.5-2007 Appendix C), where the method detection limit is 0.005 mg / g;

[0074] Carbon isotopes can be detected using isotope mass spectrometry, stable isotope mass spectrometry, and nuclear magnetic resonance spectrometry. The analytical methods after detection can refer to GB / T18340.2-2010, "Methods for Organic Geochemical Analysis of Geological Samples Part 2: Determination of Stable Carbon Isotopes in Organic Matter by Isotope Mass Spectrometry".

[0075] Nitrogen isotopes can also be detected by isotope mass spectrometry, and the analytical methods after detection can refer to the isotope mass spectrometry method described above.

[0076] Heavy metals can be detected using an inductively coupled plasma mass spectrometer (ICP-MS) 2050. The analytical methods after detection can refer to the "Determination of Total Amount of 19 Metallic Elements in Soil and Sediments by Inductively Coupled Plasma Mass Spectrometry" (HJ1315-2023).

[0077] The method detection limits (LODs) for the analysis of copper are 0.5 × 10⁻⁶, for lead are 1.0 × 10⁻⁶, for zinc are 6.0 × 10⁻⁶, for cadmium are 0.04 × 10⁻⁶, for arsenic are 0.06 × 10⁻⁶, for total mercury are 0.002 × 10⁻⁶, and for total chromium are 2.0 × 10⁻⁶.

[0078] It should be noted that if sulfides are also included in the detection indicators for sediments and feces, sulfides can also be detected by a UV-Vis spectrophotometer (Shanghai Yuanxi X-8). The analytical method after detection can refer to "Determination of Sulfides in Soil and Sediments by Methylene Blue Spectrophotometric Method" HJ833-2017, where the method detection limit is 0.3×10-6.

[0079] Furthermore, in some embodiments, the method of configuring water flow through the experimental apparatus to incorporate sediment and fecal bait, and adjusting environmental characteristics within the experimental apparatus to simulate the environment of the target area, further includes:

[0080] Adjusting the temperature of the water flow in the experimental equipment;

[0081] Adjust the aeration rate of the water flow in the experimental equipment;

[0082] Adjust the water flow, sediment, and light conditions of the feces bait in the experimental equipment.

[0083] Specifically, in the technical solution adopted in this application, the temperature and aeration rate of the water flow, as well as the light conditions of the water flow, sediment, and feces in the experimental equipment can be adjusted to simulate the dissipation efficiency and degree of feces under different environmental characteristics in the target area.

[0084] Furthermore, in some embodiments, the method for adjusting the temperature of the water flow in the experimental apparatus further includes:

[0085] Adjust the water temperature in the experimental equipment to 16℃~36℃.

[0086] Specifically, in the technical solution adopted in this application, the water flow temperature can be adjusted by the temperature control device 6. In one embodiment, the water can first be adjusted to the target temperature by the temperature control device 6, and then the water at the target temperature can be discharged into the water tank 1 of the experimental equipment, thereby forming a water flow in the water tank 1. The water flow temperature in the water tank 1 is detected to reach the expected temperature, for example, the water flow temperature in the water tank 1 is detected to be stable at 16℃, 24℃, or 36℃. In this experimental method, the water flow temperature can be adjusted to 16℃ under fixed aeration rate and constant light conditions in the experimental equipment, thereby simulating a water temperature environment of 16℃ in the target area in the experimental equipment. In one embodiment, the water flow temperature can also be adjusted to 24℃ under fixed aeration rate and constant light conditions in the experimental equipment, thereby simulating a water temperature environment of 24℃ in the target area in the experimental equipment. In one embodiment, under fixed aeration rates and constant illumination conditions in the experimental equipment, the water temperature can be adjusted to 36°C to simulate a 36°C environment in the target area. Therefore, this application proposes at least three gradient water temperatures, with a temperature difference of 8°C between each gradient, to simulate the water temperature of the target area in different seasons within the experimental equipment, thus enabling the experimental method to cover different seasonal temperature ranges during fecal bait dissipation.

[0087] Furthermore, in some embodiments, the method for adjusting the aeration rate of the water flow in the experimental apparatus further includes:

[0088] The aeration rate of the water flow in the experimental equipment was adjusted to 0%–100%.

[0089] Specifically, in the technical solution adopted in this application, an aeration device 7 can be configured in the experimental equipment. The aeration device 7 is submerged in the water flow of the experimental equipment, so as to adjust the dissolved oxygen content of the water in the experimental equipment by adjusting the power of the aeration device 7. When implementing the experimental method proposed in this embodiment, if the power of the aeration device 7 is adjusted to 0%, that is, the aeration device 7 is not activated, the aeration rate of the water flow in the experimental equipment can be considered to be 0%; if the power of the aeration device 7 is adjusted to 50%, that is, the aeration device 7 performs half-power aeration, the aeration rate of the water flow in the experimental equipment can be considered to be 50%; and if the power of the aeration device 7 is adjusted to 100%, that is, the aeration device 7 performs full-power aeration, the aeration rate of the water flow in the experimental equipment can be considered to be 100%. In this embodiment, the aeration device 7 can be a 12W four-hole oxygenation pump 71 with an air pressure of 0.018 MPa configured in the water of the experimental equipment, and an aerator 72 connected to the four-hole oxygenation pump 71. The four-hole oxygenation pump 71 is connected to an air source device for supplying oxygen, while the aerator 72 is placed at the bottom of the water tank 1, located in the first chamber 1a, so that oxygen from the air source device is discharged into the water flow in the first chamber 1a through the four-hole oxygenation pump 71 and the aerator 72. The air intake of the oxygenation pump is 0 L / min-16 L / min, where L represents liters (unit of volume) and min represents minutes (unit of time), thus indicating that the minimum air intake of the oxygenation pump is 0 liters per minute, and the maximum air intake is 16 liters per minute.

[0090] Furthermore, in some embodiments, the method for adjusting the water flow, sediment, and light conditions of the fecal bait in the experimental apparatus further includes:

[0091] The experimental equipment can be shielded from light so that the water, sediment, and feces within it are kept in a dark environment; alternatively, the experimental equipment can be made transparent so that the light source shines on the water, sediment, and feces within it.

[0092] Specifically, in the technical solution adopted in this application, the water tank 1 in the experimental equipment can be made of transparent acrylic material. This water tank 1 is used to contain sediment and fecal bait, and to supply water flow to dissipate the fecal bait. The experimental equipment can be light-shielded by covering it with a light-shielding cloth or placing it in a dark indoor environment, ensuring a light-protected environment, specifically in the water tank 1. This allows the fecal bait in the experimental equipment to dissipate under a pre-set water flow rate, selecting sediments of a specific particle size, adjusting a fixed aeration rate, and in a light-protected environment. In this embodiment, the dissipation of fecal bait under light-transmitting conditions is also included. Specifically, the water tank 1 is not light-shielded; light is transmitted into the water tank 1 through the transparent acrylic material, allowing the fecal bait in the experimental equipment to dissipate under a pre-set water flow rate, selecting sediments of a specific particle size, adjusting a fixed aeration rate, and in a light-transmitting environment. In the experimental method of this application, LED tubes can be used as light sources to illuminate the water flow, sediment, and fecal bait in the experimental equipment. Three or more LED tubes can be selected, and the electrical connection between the LED tubes and the power supply is in parallel, meaning that each LED tube can be independently controlled to turn on and off. For example, a 220V, 16W, 1600Lm LED tube, where 16W represents the LED tube power; 220 volts represents the LED tube potential difference; and 1600Lm represents the LED tube luminous flux. Therefore, in the experimental method of this application, various levels of illumination environments can be simulated by adjusting the number of LED tubes turned on.

[0093] Furthermore, in some embodiments, the method for periodically detecting various indicators of water flow in the experimental equipment and recording the dissipation of fecal bait also includes:

[0094] The primary water quality indicator in the water flow is tested regularly, with an interval of 24 hours between each test.

[0095] The primary water quality indicators in the water flow are water temperature, water color, pH value, conductivity, and dissolved oxygen content.

[0096] Specifically, in the technical solution adopted in this application, some indicators of the water flow in the experimental equipment are tested daily. This can be understood as testing every 24 hours during the experiment, and in this application, these can be defined as the first water quality indicators for subsequent reference. The first water quality indicators are water temperature, water color, pH value, conductivity, and dissolved oxygen content. It is important to note that the conductivity test is based on an assessment of the salt concentration in the water. During the experiment, most of the salt in the water comes from fish feces and uneaten food. It should be explained that during the dissipation of feces and food, some salt, such as nutrients, can be released, causing changes in the water's conductivity. Therefore, by testing the salt concentration in the water, firstly, we can understand the changes in water quality during the dissipation of feces and food, and secondly, we can determine the degree of dissipation of feces and food by detecting the salt concentration.

[0097] Furthermore, in some embodiments, the method for periodically detecting various indicators of water flow in the experimental equipment and recording the dissipation of fecal bait also includes:

[0098] The second water quality indicator in the water flow is tested regularly, with an interval of 24 hours to 600 hours between each test.

[0099] The second water quality indicators in the flow were total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH4-N), nitrite nitrogen (NO2-N), and nitrate nitrogen (NO3-N). - (N), reactive phosphate, chemical oxygen demand and heavy metals.

[0100] Specifically, in the technical solution adopted in this application, the various water flow indicators in the experimental equipment also include some indicators with gradually extended detection intervals. This can be understood as the detection time for these indicators being gradually extended during the experiment. In this application, these can be defined as second water quality indicators for subsequent reference. The second water quality indicators are: Total Organic Carbon (TOC), Total Nitrogen (TN), Total Phosphorus (TP), Ammonia Nitrogen (NH4-N), Nitrite Nitrogen (NO2-N), and Nitrate Nitrogen (NO3-N). - The second water quality indicator includes (N), reactive phosphate, chemical oxygen demand, and heavy metals. Heavy metals in the second water quality indicator include, but are not limited to, copper, lead, zinc, cadmium, arsenic, total mercury, total chromium, nickel, and total vanadium. In this embodiment, the interval between each test of the second water quality indicator can be gradually extended from 24 hours to 48 hours to 600 hours. For example, the interval can be changed from 24 hours to 48 hours, 72 hours, 120 hours, 240 hours, 360 hours, 480 hours, and 600 hours.

[0101] In this application, the various water quality indicators can be tested in the following manner:

[0102] Water temperature can be measured using a water thermometer, and the analysis method after measurement can refer to the "Water Quality and Water Temperature Measurement Method" (GB13195-91).

[0103] Water color can be detected and the results recorded visually, while the analysis methods after detection can refer to "Determination of Water Color" (GB11903-89);

[0104] pH value can be measured using a portable pH meter, and the analysis method after measurement can refer to the pH meter method "Electrode Method for Determination of pH Value in Water" (HJ1147-2020).

[0105] Dissolved oxygen content can be detected using a portable DO meter, and the analytical methods after detection can refer to "Electrochemical Probe Method for Determination of Dissolved Oxygen in Water" (HJ506-2009);

[0106] The detection and analysis methods for total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) can be applied to sediments and feces, so they will not be described in detail here.

[0107] Ammonia nitrogen (NH4-N) can be detected by a UV-Vis spectrophotometer (X8 Shanghai Yuanxi), and the analytical method after detection can refer to "Determination of Ammonia Nitrogen in Water by Salicylic Acid Spectrophotometric Method" (HJ536-2009), where the method detection limit is 0.005 mg / L;

[0108] Nitrite nitrogen (NO2-N) can be detected by a UV-Vis spectrophotometer (X8 Shanghai Yuanxi), and the analytical method after detection can refer to the "Determination of Nitrite Nitrogen in Water - Spectrophotometric Method" (GB7493-87), where the method detection limit is 0.0009 mg / L;

[0109] Nitrate nitrogen (NO3) - -N) can be detected by a UV-Vis spectrophotometer (X8 Shanghai Yuanxi), and the analytical method after detection can refer to "Determination of Nitrate Nitrogen in Water by UV Spectrophotometry (Trial)" (HJ / T346─2007), where the method detection limit is 0.003 mg / L;

[0110] Reactive phosphate can be detected by a UV-Vis spectrophotometer (X8 Shanghai Yuanxi), and the analytical method after detection can refer to "Determination of Phosphate and Total Phosphorus in Water - Continuous Flow-Ammonium Molybdate Spectrophotometric Method" (HJ670-2013), where the method detection limit is 0.001 mg / L;

[0111] Chemical oxygen demand (COD) can be detected by a UV-Vis spectrophotometer (X8 Shanghai Yuanxi). The analytical method after detection can refer to the "Determination of Chemical Oxygen Demand in Water - Rapid Digestion Spectrophotometric Method" (HJ / T399-2007), where the method detection limit is 0.15 mg / L.

[0112] Heavy metals can be detected using an inductively coupled plasma mass spectrometer (ICP-MS) 2050. The analytical methods after detection can be found in the "Determination of 65 Elements in Water by Inductively Coupled Plasma Mass Spectrometry" (HJ700-2014).

[0113] The method detection limits for copper analysis are 0.2 μg / L, for lead analysis are 0.03 μg / L, for zinc analysis are 3.1 μg / L, for cadmium analysis are 0.01 μg / L, for mercury analysis are 0.007 μg / L, for arsenic analysis are 0.5 μg / L, for total chromium analysis are 0.4 μg / L, and for nickel analysis are 0.4 μg / L.

[0114] Five-day biochemical oxygen demand (BOD5) can be detected by a flow injection BOD online monitor. The analytical method after detection can refer to the "Determination of Five-Day Biochemical Oxygen Demand (BOD5) in Water - Dilution and Inoculation Method" (HJ505-2009), where the method detection limit is 1 mg / L.

[0115] Suspended solids can be detected using an SQP electronic balance (225D-1CN), and the analytical method after detection can be found in "Determination of Suspended Solids in Water by Gravimetric Method" (GB11901-89), where the method detection limit is 2 mg / L.

[0116] Chlorophyll a can be detected by a UV-Vis spectrophotometer (X8 Shanghai Yuanxi), and the analytical method after detection can refer to the "Spectrophotometric Method for Determination of Chlorophyll a in Water Quality" (HJ897-2017)(8.2);

[0117] Sulfides can be detected using a UV-Vis spectrophotometer (X8 Shanghai Yuanxi), and the analytical method after detection can refer to "Determination of Sulfides in Water by Methylene Blue Spectrophotometric Method" (HJ1226—2021), where the method detection limit is 0.2 μg / L.

[0118] In the experimental method of this application, the measured water quality indicators can be compared with the indicators of tap water to obtain the impact of each time period during the fecal bait dissipation process on water quality under the experimentally set environmental conditions.

[0119] Furthermore, in some embodiments, the method for periodically detecting various indicators of water flow in the experimental equipment and recording the dissipation of fecal bait also includes:

[0120] The first and second water quality indicators of the water flow are compared with the various indicators of tap water to obtain the water quality differences between the water flow and tap water.

[0121] Furthermore, in some embodiments, the method for obtaining the second parameter by detecting various indicators of sediment and fecal bait in the experimental equipment after the experiment is terminated further includes:

[0122] This experiment will terminate when one of the following conditions is met:

[0123] Visually, the fecal bait in the experimental equipment has completely dissipated;

[0124] The experiment to observe the dissipation process of fecal bait lasted for three months;

[0125] Among the methods for regularly testing various indicators of water flow in experimental equipment and recording the dissipation of feces and bait, the test results of water flow indicators are stable.

[0126] In the method of regularly testing various indicators of water flow in experimental equipment and recording the dissipation of feces and bait, the test results of water flow indicators are no different from those of tap water.

[0127] Example 2

[0128] Reference Figures 1 to 3 As shown, an experimental device for observing the dissipation process of fecal bait is proposed in an embodiment of this application. This experimental device may include: a water tank 1, equipped with an inlet 11 and an outlet 12, so that water flows through the inlet 11 and outlet 12 within the water tank 1; the water tank 1 has a first chamber 1a, a second chamber 1b, and a third chamber 1c, the first chamber 1a being connected to the second chamber 1b, the second chamber 1b being located between the first chamber 1a and the third chamber 1c, the first chamber 1a being connected to the inlet 11, and the third chamber 1c being connected to the outlet 12. Chamber 1c is connected to outlet 12. A sedimentation layer 9 is laid at the bottom of the second chamber 1b for placing manure. A flow straightener 2 is disposed between the first chamber 1a and the second chamber 1b to regulate the water flow rate from the first chamber 1a into the second chamber 1b. An overflow plate 3 is disposed between the second chamber 1b and the third chamber 1c. An overflow gap is provided above the overflow plate 3. The second chamber 1b and the third chamber 1c are connected through the overflow gap so that the overflow plate 3 can limit the upper limit of the water level in the second chamber 1b.

[0129] Specifically, in the technical solution adopted in this application, the water tank 1 includes a first chamber 1a with an inlet 11 for receiving experimental water supplied by a water source, a second chamber 1b located between the first chamber 1a and the third chamber 1c, the second chamber 1b serving as a dissipation area for feces bait, thus requiring a sedimentation layer 9 to be laid at the bottom of the second chamber 1b before the experiment, and feces bait required for the experiment to be placed on the sedimentation layer 9; the third chamber 1c is connected to an outlet 12, so that water flowing from the second chamber 1b into the third chamber 1c can be discharged from the water tank 1 through the outlet 12. The rectifier plate 2 is fixedly installed on the side wall of the water tank 1 to form a first chamber 1a and a second chamber 1b in the water tank 1 through the separation of the rectifier plate 2. The flow rate of water flowing from the first chamber 1a into the second chamber 1b can be adjusted by the rectifier plate 2. The overflow plate 3 is located between the second chamber 1b and the third chamber 1c. An overflow gap is reserved above the overflow plate 3 to connect the second chamber 1b and the third chamber 1c. When the water level in the second chamber 1b is higher than the overflow plate 3, the excess water flows into the third chamber 1c through the overflow gap. The water in the third chamber 1c can be discharged from the experimental device through the outlet 12 so that the experimental device of this application can be continuously flowed by water to simulate the underwater flowing water environment of the target area in the outside world.

[0130] Furthermore, refer to Figure 1 As shown, the water outlet direction is towards the rectifier plate; the experimental device for observing the dissipation process of feces bait also includes: a baffle plate 4, which is set in the first chamber 1a. The baffle plate 4 is located between the water inlet 11 and the rectifier plate 2, so that one side surface of the baffle plate 4 faces the water inlet 11.

[0131] Specifically, in the technical solution adopted in this application, when the water outlet of the water tank 1 is directed toward the rectifier plate 2, the baffle plate 4 in the first chamber 1a can block the water outlet direction of the inlet 11, so that the water entering the first chamber 1a from the inlet 11 can be sprayed onto the baffle plate 4, effectively preventing the water entering through the inlet 11 from passing through the rectifier plate 2 and directly flushing the sediment layer 9 and feces in the second chamber 1b.

[0132] Furthermore, refer to Figure 1 As shown, in some embodiments, the rectifier plate 2 is provided with a plurality of holes 20 consisting of drainage holes 200, and each hole 20 is arranged along the first direction of the rectifier plate 2 to form a rectangular array; the first chamber 1a and the second chamber 1b are connected through each hole 20.

[0133] Specifically, in the technical solution adopted in this application, the rectifier plate 2 is vertically installed between the first chamber 11 and the second chamber 12. Several sets of holes 20 arranged along a first direction of the rectifier plate 2 are formed on the rectifier plate 2. The first direction can be either the length or width of the rectifier plate 2. Specifically, it depends on whether the width of the water tank 1 is greater than its height. If the width of the water tank 1 is greater than its height, the first direction is the length of the rectifier plate 2; conversely, if the width of the water tank 1 is less than its height, the first direction is the width of the rectifier plate 2. The hole array 20 consists of drain holes 200 arranged in a line along the width of the rectifier plate 2, thus forming a rectangular array of drain holes 200 on the rectifier plate 2. The first chamber 1a and the second chamber 1b are connected through the hole array 20, allowing water in the first chamber 1a to drain into the second chamber 1b through the drain holes 200. Because the water in the first chamber 1a is mitigated by the baffle plate 4, the water in the first chamber 1a can flow smoothly into the second chamber 1b through the corresponding drain hole 200, so as to avoid the water flow impact being too strong and washing away the sediment layer 9 in the second chamber 1b, thus disrupting the natural dissipation process of the feces.

[0134] Reference Figure 2 As shown, in some embodiments, each drain hole 200 can be of the same size. During the experiment, the number of activated drain holes 200 can be increased by gradually raising the water level in the first chamber 1a to regulate the flow rate of water into the second chamber 1b. It can be understood that the higher the water level in the first chamber 1a, the more drain holes 200 are activated, and thus the greater the flow rate of water into the second chamber 1b.

[0135] Furthermore, refer to Figure 3 As shown, in some embodiments, in each group of holes 20, the size of each drain hole 200 gradually decreases along a second direction perpendicular to the first direction on the rectifier plate 2.

[0136] Specifically, in the technical solution adopted in this application, when the first direction is the length direction of the rectifier plate 2, the size of the drain holes 200 in each hole row 20 can be set to gradually decrease along the width direction of the rectifier plate 2, which is the second direction; conversely, when the first direction is the width direction of the rectifier plate 2, the size of the drain holes 200 in each hole row 20 can be set to gradually decrease along the length direction of the rectifier plate 2, which is the second direction. When the rectifier plate 2 is located between the first chamber 1a and the second chamber 1b, the size of the drain holes 200 in the hole row 20 can gradually decrease from top to bottom or from bottom to top. This allows the water flowing into the second chamber 1b through the rectifier plate 2 to have more selectable flow conditions when the water level in the first chamber 1a is raised by increasing the drainage volume, so that the experimental equipment in this application can simulate more external water flow environments.

[0137] Furthermore, refer to Figure 1 As shown, in some embodiments, it further includes: a water supply pipeline 5 for connecting to a water source, and a water inlet 11 for connecting to a water source through the water supply pipeline 5; and a temperature control device 6, configured on the water supply pipeline 5, for adjusting the water to the target temperature and then discharging it into the first chamber 1a through the water supply pipeline 5.

[0138] Specifically, in the technical solution adopted in this application, the experimental water can be tap water, and the water supply pipeline 5 can be connected to a tap water source to meet the water requirements of the experimental equipment. Before the tap water enters the first chamber 1a, the water temperature needs to be set to meet the requirements of simulating the external environment. Therefore, the tap water needs to be temperature-controlled by a temperature control device 6 before entering the first chamber 1a. In this embodiment, the temperature control device 6 is configured on the water supply pipeline 5 so that the tap water, after being temperature-controlled, passes sequentially through the water supply pipeline 5 and the inlet 11 until it is discharged into the first chamber 1a. The temperature control device 6 has both cooling and heating functions, allowing adjustment of the water temperature according to experimental needs. For example, the water temperature can be adjusted to 16℃, 24℃, or 36℃. Normally, tap water will not exceed 24℃. If the experiment requires water at 24℃ or 36℃, the heating function of the temperature control device can be activated to heat the tap water. The water temperature in the first chamber 1a can then be checked to ensure it reaches the desired temperature. However, in some cases, the temperature of room-temperature tap water may exceed 16℃. In such cases, the cooling function of the temperature control device can be activated to cool the tap water. The water temperature in the first cavity can then be checked to ensure it reaches the desired temperature. Of course, different water temperatures can also be set using the cooling and heating functions of the temperature control device during the experiment, depending on actual needs.

[0139] Furthermore, refer to Figure 1 As shown, in some embodiments, a ball valve 51 and a flow meter 52 are provided on the water supply pipeline 5. The ball valve 51 can close the water supply pipeline 5, and the flow meter 52 is used to record the flow rate of water in the water supply pipeline 5.

[0140] Specifically, in the technical solution adopted in this application, the ball valve 51 can control the opening of the water supply pipeline 5 to adjust the water supply flow rate of the water supply pipeline 5, so that the water supply of the experimental equipment can be adjusted through the ball valve 51, while the flow meter 52 can detect the current flow parameters of the water supply pipeline 5, so that the flow rate of the water supply pipeline 5 can be converted into a visual parameter through the flow meter 52, so that the operator can observe the parameters of the flow meter 52 and adjust the water supply pipeline 5 to the target flow rate through the ball valve 51.

[0141] Reference Figure 1As shown, in one embodiment, the water supply pipeline 5 may include a first pipeline section and a second pipeline section. The first pipeline section connects the water source and the inlet 61 of the temperature control device 6, and the second pipeline section connects the outlet and inlet 11 of the temperature control device 6. A ball valve 51 may be configured on the first pipeline section to control the flow rate of tap water entering the temperature control device 6 according to actual needs by adjusting the opening of the ball valve 51. A flow meter 52 may be configured on the second pipeline section to record the flow rate parameters discharged into the first chamber 1a through the temperature control device 6.

[0142] In one embodiment, both the ball valve 51 and the flow meter 52 can be configured on the second pipeline section. It should be noted that in this embodiment, the ball valve 51 is set between the outlet 62 and the flow meter 52 so as to control the drainage volume through the temperature control device 6, that is, the flow rate of water at the target temperature. The flow meter 52 is used to record the flow parameters of the temperature control device 6. Since the second pipeline section connects the outlet 62 and the inlet 11, the flow parameters are also the flow parameters of the water discharged into the first chamber 1a.

[0143] Furthermore, refer to Figure 1 As shown, in some embodiments, it further includes: an aeration device 7 disposed in the first chamber 1a, the aeration device 7 having at least two air intake settings to adjust the dissolved oxygen content of the water in the first chamber 1a.

[0144] Specifically, in the technical solution adopted in this application, an aeration device 7 is also provided in the first chamber 1a. The aeration device 7 can be placed on the bottom wall of the water tank 1. The aeration device 7 can deliver oxygen to the water in the water tank 1 to regulate the dissolved oxygen content in the water flow. In this embodiment, the aeration device 7 has at least two air intake levels, which can be understood as the output power of the aeration device 7 having two adjustable levels, so that the aeration device 7 has three modes when supplying oxygen: the aeration device 7 is turned off and no oxygen is supplied to the water, and two different output powers for oxygen supply. For example, if the two output powers of the aeration device 7 are half-power aeration and full-power aeration, then the aeration rate in the water is 0% when the aeration device 7 is turned off, 50% when the aeration device 7 is turned on at half-power aeration, and 100% when the aeration device 7 is turned on at full-power aeration.

[0145] In one embodiment, the aeration device 7 may retain a 12W power, 0.018MPa four-hole aerator 71 and an aerator 72 connected to the four-hole aerator 71. The four-hole aerator 71 is connected to an air source device for supplying oxygen, while the aerator 72 is placed at the bottom of the water tank 1, located in the first chamber 1a, so that oxygen from the air source device is discharged into the water flow in the first chamber 1a through the four-hole aerator 71 and the aerator 72. The adjustable air intake of the aerator is 0L / min-16L / min, where L represents liters (unit of volume) and min represents minutes (unit of time); thus, the air intake of the aeration device 7 at half power is 8L / min, and the air intake of the aeration device 7 at full power is 16L / min.

[0146] Furthermore, refer to Figure 1 As shown, in some embodiments, the sidewall of the water tank 1 corresponding to the second chamber 1b is made of transparent acrylic sheet.

[0147] Furthermore, refer to Figure 1 As shown, in some embodiments, it further includes: a lighting device 8; the lighting direction of the lighting device 8 corresponds to that of the second chamber 1b, and the lighting device 8 is used to simulate a lighting environment in the second chamber 1b.

[0148] Specifically, in the technical solution adopted in this application, the water tank 1 can be a rectangular box made of transparent acrylic material. Acrylic material is not only sturdy and durable but also has good light transmittance, facilitating the simulation of the lighting environment of the target area within the water tank 1. In one embodiment, only the side wall of the water tank 1 corresponding to the second chamber 1b can be made of transparent acrylic sheet. In this embodiment, a lighting device 8 can be arranged above the water tank 1. The light from this lighting device 8 can cover the entire water tank 1, especially the second chamber 1b within the water tank 1, thereby simulating the lighting environment of the target area during the experiment.

[0149] Furthermore, refer to Figure 1 As shown, in some embodiments, the illumination device 8 includes at least two light source components, which are electrically connected to a power source in parallel to adjust the illumination intensity in the second chamber 1b by independently turning the two light source components on and off.

[0150] Specifically, in the technical solution adopted in this application, the light source component can be an LED tube, and the electrical connection between the LED tube and the power supply is in parallel, meaning that each LED tube can be independently controlled to turn on and off. For example, a 220V, 16W, 1600Lm LED tube, where 16W represents the LED tube power; 220V represents the LED tube potential difference; and 1600Lm represents the LED tube luminous flux. Therefore, in the experimental method of this application, various levels of illumination environments can be simulated by adjusting the number of LED tubes turned on.

[0151] Furthermore, in some embodiments, it also includes: a light intensity measuring instrument, disposed in the second chamber 1b, for monitoring the light intensity above and below the water in the second chamber 1b.

[0152] Specifically, in the technical solution adopted in this application, two light intensity measuring instruments can be installed in the second chamber 1b. The two light intensity measuring instruments can be fixed on the side wall of the water tank 1. One of the light intensity measuring instruments is installed at a height higher than the overflow plate 3 to monitor the light intensity on the water surface in the second chamber 1b, while the other light intensity measuring instrument is installed at a height lower than the overflow plate 3 to monitor the light intensity underwater in the second chamber 1b.

[0153] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of those different embodiments or examples.

[0154] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "a plurality of" means two or more, unless otherwise explicitly specified.

[0155] Any process or method description in the flowchart or otherwise herein can be understood as representing a module, segment, or portion of code comprising one or more executable instructions for implementing a particular logical function or process. Furthermore, the scope of the preferred embodiments of this application includes additional implementations in which functions may be performed not in the order shown or discussed, including substantially simultaneously or in reverse order depending on the functionality involved.

[0156] The logic and / or steps represented in the flowchart or otherwise described herein, for example, can be considered as a sequenced list of executable instructions for implementing logical functions, and can be embodied in any computer-readable medium for use by, or in conjunction with, an instruction execution system, apparatus or device (such as a computer-based system, a processor-included system or other system that can fetch and execute instructions from, an instruction execution system, apparatus or device).

[0157] It should be understood that various parts of this application can be implemented using hardware, software, firmware, or a combination thereof. In the above embodiments, multiple steps or methods can be implemented using software or firmware stored in memory and executed by a suitable instruction execution system. All or part of the steps of the methods in the above embodiments can be implemented by a program instructing related hardware, the program being stored in a computer-readable storage medium, which, when executed, includes one or a combination of the steps of the method embodiments.

[0158] Furthermore, the functional units in the various embodiments of this application can be integrated into a processing module, or each unit can exist physically separately, or two or more units can be integrated into a module. The integrated module can be implemented in hardware or as a software functional module. If the integrated module is implemented as a software functional module and sold or used as an independent product, it can also be stored in a computer-readable storage medium. This storage medium can be a read-only memory, a disk, or an optical disk, etc.

[0159] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this application, and these should all be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. An experimental method for observing the dissipation process of fecal bait, characterized in that, include: Obtain the required sediment and fecal bait, and test various indicators of the sediment and fecal bait to obtain the first parameter; The fecal bait was placed on the sediment laid in the experimental equipment; A water flow passing through the sediment and the fecal bait is configured in the experimental device, and the environmental characteristics in the experimental device are adjusted to simulate the environment of the target area; Regularly test various indicators of the water flow in the experimental equipment and record the dissipation of the feces bait; After the experiment was terminated, various indicators of the sediment and the fecal bait in the experimental equipment were tested to obtain the second parameter; The second parameter is compared with the first parameter to obtain the dissipation of the sediment and the fecal bait under the simulated environment in the experimental equipment; The indicators to be tested for the sediment and the fecal bait include: total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), crude protein, ash content, moisture content, carbon isotopes, nitrogen isotopes, and heavy metals. The method of configuring water flow through the sediments and fecal bait in the experimental apparatus and adjusting the environmental characteristics of the experimental apparatus to simulate the environment of the target area further includes: Adjust the temperature of the water flow in the experimental apparatus; Adjust the aeration rate of the water flow in the experimental equipment; Adjust the water flow, sediment, and light conditions of the feces in the experimental equipment; The method for adjusting the water flow, sediment, and light conditions of the feces in the experimental equipment further includes: The experimental equipment is shielded from light so that the water flow, sediment, and fecal bait within the equipment are kept in a dark environment; or, The experimental equipment is made transparent, and a light source is irradiated onto the water flow, the sediment, and the feces in the experimental equipment. The method for periodically detecting various indicators of the water flow in the experimental equipment and recording the dissipation of the fecal bait further includes: The first and second water quality indicators of the water flow are compared with the various indicators of tap water to obtain the water quality difference between the water flow and the tap water.

2. The experimental method for observing the dissipation process of fecal bait according to claim 1, characterized in that, The method for adjusting the temperature of the water flow in the experimental apparatus further includes: The water temperature in the experimental equipment was adjusted to 16℃~36℃.

3. The experimental method for observing the dissipation process of fecal bait according to claim 1, characterized in that, The method for adjusting the aeration rate of the water flow in the experimental equipment further includes: The aeration rate of the water flow in the experimental equipment was adjusted to 0%~100%.

4. The experimental method for observing the dissipation process of fecal bait according to claim 1, characterized in that, The method for periodically detecting various indicators of the water flow in the experimental equipment and recording the dissipation of the fecal bait further includes: The first water quality index in the water flow is tested regularly, with an interval of 24 hours between each test. The primary water quality indicators in the water flow are water temperature, water color, pH value, conductivity, and dissolved oxygen content.

5. The experimental method for observing the dissipation process of fecal bait according to claim 4, characterized in that, The method for periodically detecting various indicators of the water flow in the experimental equipment and recording the dissipation of the fecal bait further includes: The second water quality index in the water flow is tested regularly, with an interval of 24 hours to 600 hours between each test. The second water quality indicators in the water flow are total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH4-N), nitrite nitrogen (NO2-N), and nitrate nitrogen (NO3). - -N, reactive phosphate, chemical oxygen demand, and heavy metals.

6. The experimental method for observing the dissipation process of fecal bait according to claim 1, characterized in that, The method for obtaining the second parameter by detecting various indicators of the sediment and the fecal bait in the experimental equipment after the experiment is terminated further includes: This experiment will terminate when one of the following conditions is met: Visually, the fecal bait in the experimental equipment has completely dissipated; The experiment to observe the dissipation process of fecal bait lasted for three months; In the method of periodically detecting various indicators of the water flow in the experimental equipment and recording the dissipation of the feces, the detection results of the water flow indicators are stable. In the method of periodically testing various indicators of the water flow in the experimental equipment and recording the dissipation of the fecal bait, the water quality test results are no different from those of tap water.

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