Euonymus antibody dilution solution reagent strip preparation device and preparation method thereof

Abstract

The present application relates to the technical field of europium-labeled antibody preparation, and discloses a europium-labeled antibody dilution solution reagent strip preparation device and a preparation method thereof, which comprises a support frame, a mixing tank is mounted on one side of the top of the support frame, a reagent strip is arranged in the support frame, a discharge pipe is fixed to the bottom of the mixing tank, and a smearing assembly is arranged at the bottom of the discharge pipe, the smearing assembly comprises a guide frame fixed to the bottom of the discharge pipe, sliding grooves are formed in the two ends of the guide frame, sliding blocks are slidably arranged on the inner walls of the sliding grooves, sealing plates are fixed to the inner walls of the two ends of the guide frame and located on one side of the two sliding blocks, and smearing roller pieces are rotatably connected to the bottom of one side of the two sealing plates, the smearing roller pieces have a stirring function during smearing, a certain extrusion effect is formed on the reagent strip and the liquid absorption layer, the surface of the reagent strip is better adapted, the uniformity of smearing is ensured, and the quality of reagent strip preparation is improved.

Description

Technical Field

[0001] This invention relates to the field of europium-labeled antibody preparation technology, and in particular to an apparatus and method for preparing europium-labeled antibody dilution solution reagent strips. Background Technology

[0002] Europium-labeled antibodies are biomolecular probes formed by conjugating the rare earth element europium with an antibody. The principle behind this is the use of europium's unique fluorescence properties. Through a specific chemical reaction, europium ions or their chelates are linked to the antibody molecule without affecting the antibody's immunomodulatory activity and specificity. When exposed to excitation light, europium emits characteristic fluorescence, which can be sensitively detected by fluorescence detection instruments. In immunoassay techniques, europium-labeled antibodies are widely used in time-resolved fluorescence immunoassay and other detection methods due to their advantages such as long fluorescence lifetime, large Stokes shift, and sharp emission spectrum. Europium-labeled antibody dilution solution test strips, as a convenient and rapid detection tool, are of great significance for on-site testing and large-scale screening.

[0003] Currently, traditional europium-labeled antibody dilution solution test strips have many problems in their preparation process: existing preparation devices have low automation levels, and manual operation steps are cumbersome, resulting in low preparation efficiency and easy introduction of human error. More importantly, during the coating process of loading europium-labeled antibody dilution solution onto the test strip, it is difficult to accurately control the coating amount of europium-labeled antibody dilution solution, leading to uneven antibody concentration on the test strip and affecting the accuracy and repeatability of the test results. Therefore, there is an urgent need to propose a europium-labeled antibody dilution solution test strip preparation device and its preparation method to improve the above problems. Summary of the Invention

[0004] The technical problem to be solved by the present invention is that, in the process of loading europium-labeled antibody dilution solution onto the test strip, it is difficult to accurately control the amount of europium-labeled antibody dilution solution applied, resulting in uneven antibody concentration on the test strip and affecting the accuracy and repeatability of the test results.

[0005] To address the aforementioned problems, this invention provides a device for preparing europium-labeled antibody dilution solution reagent strips, comprising a support frame, a mixing tank mounted on one side of the top of the support frame, and reagent strips disposed within the support frame. A discharge pipe is fixed to the bottom of the mixing tank, and an application assembly is disposed at the bottom of the discharge pipe. The application assembly includes a guide frame fixed to the bottom of the discharge pipe, with grooves at both ends of the guide frame. Slider blocks slide along the inner walls of the grooves. Sealing plates, conforming to the inner walls of both ends of the two sliders, are fixed to one end of each slider. Application rollers are rotatably connected to one bottom side of each of the two sealing plates. A lifting plate is fixed to the other end of one of the sliders. One end of the guide frame... An electric push rod for adjusting the height of the lifting plate is installed on the outer wall of the end. The coating roller includes a central shaft, and the outer wall of the central shaft is provided with an anti-permeability layer. The outer wall of the anti-permeability layer is provided with a liquid-absorbing layer. The inner wall of the anti-permeability layer and the outer wall of the central shaft are provided with soft rubber seats that are evenly distributed. Multiple rows of E-shaped tubes that are evenly distributed are fixed to the outer wall of the central shaft, and the positions of the E-shaped tubes and the soft rubber seats are staggered. The E-shaped tubes are filled with inert gas. Movable rods are inserted into the three inner walls of the E-shaped tubes. A support rod is fixed to one end of each movable rod. A piston is fixed to one end of the outer wall of each movable rod. A spring is installed at the middle position between the other end of the movable rod and the inner wall of the E-shaped tube.

[0006] The present invention is further configured such that an inlet hole communicating with the discharge pipe is provided at the top center of the guide frame, and an L-shaped connecting plate is fixed on one side of each of the two sealing plates, and a sealing plate that fits against the bottom of the inlet hole is fixed on the top of each L-shaped connecting plate.

[0007] The invention is further configured such that the mixing tank is fixedly composed of an outer shell and an inner liner, and an agitator is installed in the middle of the mixing tank. The discharge pipe is fixed in the middle of the bottom of the inner liner and extends out of the outer shell. The gap between the outside of the inner liner and the inside of the outer shell is designed as a heat preservation cavity.

[0008] The present invention is further configured such that a drying chamber and a cleaning component are sequentially installed on the top of the support frame near the middle position, and a first side plate is fixed to both ends of one side of the drying chamber, and a second side plate is fixed to both ends of one side of the cleaning component and the top of the support frame. A first rotating shaft is rotatably connected to one end of the two first side plates, and a second rotating shaft is rotatably connected to the bottom of one end of the two second side plates. An installation groove is provided on both sides of the top of the two second side plates, and an unwinding machine and a winding machine are respectively installed on the inner wall of the installation groove. The reagent strip is wound on the unwinding machine, the winding machine, the first rotating shaft, and the second rotating shaft.

[0009] The invention is further configured such that a support platform is fixedly installed on one side of each of the two first side plates, and the support platform is located directly below the guide frame. An arc-shaped groove is provided at the top center of the support platform, and the arc-shaped groove is located directly below the coating roller. A collection box is provided inside the support frame, and the collection box is located below the first side plates and the support platform.

[0010] The present invention is further configured such that the cleaning assembly includes a cleaning box installed on one side of the drying box and the top of the support frame, and a plurality of support rollers are rotatably connected to the inner wall of the cleaning box. The reagent strip is wound around the support rollers. Inlet and outlet are provided on both sides of the cleaning box and on the top and bottom of both sides of the drying box. The reagent strip passes through the inlet and outlet. A drain pipe is fixed on the bottom of one side of the cleaning box, and a drain valve is installed at one end of the drain pipe.

[0011] The invention is further configured such that two mounting holes are provided on one side of the cleaning box near the middle position, and the inner walls of the mounting holes are respectively rotatably connected to the drive shaft of the connecting shaft through the sealed bearing. One end of the drive shaft is fixedly connected to one end of one of the support rollers, and a cleaning roller brush is fixedly installed on one end of the connecting shaft. The cleaning roller brush is attached to the reagent strip. Gears are installed on the other end of the connecting shaft and the other end of the drive shaft, and the two gears mesh with each other.

[0012] The invention is further configured such that a vacuum pump and a low-temperature heater are respectively installed on one side of the drying chamber, and an L-shaped square tube is installed on one side of the top of the drying chamber and one side of the outer shell, and the drying chamber, the L-shaped square tube and the heat preservation cavity are connected.

[0013] The invention is further configured such that a cutting machine is installed on the top side of the two second side plates near the winding machine, and a cutting table is installed on the bottom side of one side of the two second side plates. A cutting opening is provided in the middle of the cutting table, and the cutting table is located directly below the cutting machine.

[0014] A method for preparing europium-labeled antibody dilution solution reagent strips, applied to an apparatus for preparing europium-labeled antibody dilution solution reagent strips, includes the following steps:

[0015] Step 1: Prepare the europium-labeled antibody dilution solution according to the following proportions: Tris-HCl buffer, pH 7.2-7.6, concentration 20-50 mmol / L; bovine serum albumin, mass fraction 0.5%-2%; trehalose, mass fraction 1%-5%; ProClin 300 preservative, volume fraction 0.05%-0.2%; the remainder is deionized water.

[0016] Step 2: Preparation of the carrier: Nitrocellulose membrane is selected as the carrier for the reagent strip;

[0017] Step 3: Dilution solution: Inject all components of the prepared europium-labeled antibody dilution solution into the mixing vessel and mix thoroughly for 20-30 minutes using the stirrer inside the mixing vessel;

[0018] Step 4, even coating: Turn off the stirrer, wrap the reagent strip around the entire preparation device, clean the surface of the reagent strip with the cleaning component, and after drying, pass it from the bottom of the mixing tank and use the coating component to evenly coat the europium-labeled antibody reagent onto the reagent strip.

[0019] Step 5: Continue drying: Pass the applied reagent strip through the drying oven at a temperature of 45-60℃;

[0020] Step 6, Cutting and Packaging: Cut the dried reagent strips to the required length using a cutting machine. Place the cut reagent strips in a sealed bag, add desiccant, and vacuum pack.

[0021] In summary, by adopting the above structure, the present invention has the following advantages compared with the prior art:

[0022] 1. In this invention, the height of the lifting plate is adjustable by an electric push rod in the coating assembly, thereby controlling the position of the sealing plate and the coating roller. When reagent needs to be applied, the coating roller can be lowered for application; when not needed, the sealing plate can be raised for sealing, preventing reagent dripping and contamination. This improves the controllability and accuracy of the coating process and facilitates operation. Moreover, the liquid-absorbing layer in the coating roller can effectively absorb and apply the reagent, and the anti-permeability layer can prevent the reagent from penetrating to the central shaft, extending the service life of the coating roller. Its internal soft rubber seat, E-shaped tube, movable rod, support rod, and spring structure, combined with the arc-shaped groove, give the coating roller a prying performance during the coating process, forming a certain squeezing effect on the reagent strip and the liquid-absorbing layer, better adapting to the surface of the reagent strip, ensuring the uniformity of the coating, and improving the quality of reagent strip preparation.

[0023] 2. In this invention, the arrangement of components such as the unwinding machine, the winding machine, the first rotating shaft, and the second rotating shaft enables the reagent strips to be smoothly transported in the preparation device. The entire preparation process is continuous and efficient. The reagents dripping during the preparation process are collected by the collection box to keep the inside of the device clean. The prepared reagent strips can be accurately cut using the cutting machine and the cutting table to obtain reagent strip products that meet the specifications.

[0024] 3. In this invention, the cleaning roller brush in the cleaning component adheres to the reagent strip and rotates under the transmission of gears, which can effectively clean impurities on the surface of the reagent strip, ensure the cleanliness of the reagent strip surface, improve the detection accuracy of the reagent strip, and dry and heat the cleaned reagent strip in the drying oven, so that the reagent can better penetrate into the reagent strip when applying the reagent later, improve the performance and stability of the reagent strip, and dry the reagent strip after applying the reagent, so that the moisture in the reagent can be fully evaporated to form a dry reagent strip, so as to ensure that the components in the reagent can firmly adhere to the nitrocellulose membrane.

[0025] 4. In this invention, the L-shaped square tube connects the drying chamber and the insulation chamber, which can make reasonable use of the heat generated during the drying process to heat the inside of the mixing tank, improve the mixing effect and energy utilization efficiency, and at the same time help maintain the temperature stability of the reagents inside the mixing tank.

[0026] 5. In this invention, Tris-HCl buffer, bovine serum albumin, trehalose, ProClin300 preservative, and deionized water are used as the dilution solution components for europium-labeled antibodies. This maintains a stable pH value, providing a suitable acid-base environment for the europium-labeled antibodies. The bovine serum albumin also prevents non-specific adsorption of the europium-labeled antibodies, protecting antibody activity. Trehalose provides excellent moisture retention and protection for biomolecules, enhancing the stability of the europium-labeled antibodies. The ProClin300 preservative effectively inhibits microbial growth, extending the shelf life of the test strips. Furthermore, the nitrocellulose membrane used as the carrier of the test strips has excellent adsorption properties and liquid flow rate, ensuring the accuracy and sensitivity of the test strips during the detection process. Attached Figure Description

[0027] Figure 1 This is a three-dimensional structural schematic diagram of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0028] Figure 2 This is a front view of an apparatus for preparing europium-labeled antibody dilution solution reagent strips according to the present invention;

[0029] Figure 3 This is a schematic diagram of the first and second rotating shafts of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0030] Figure 4 This is a schematic diagram of the cleaning component structure of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0031] Figure 5 This is a schematic diagram of the mounting groove structure of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0032] Figure 6 This is a schematic diagram of the outer shell and inner liner of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0033] Figure 7 This is a schematic diagram of the feed frame and electric push rod structure of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0034] Figure 8 This is a schematic diagram of the coating roller and sealing plate structure of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention;

[0035] Figure 9 This is a schematic diagram of the central axis and the absorbent layer structure of a europium-labeled antibody dilution solution reagent strip preparation device of the present invention;

[0036] Figure 10 This is a front cross-sectional view of the applicator roller of a europium-labeled antibody dilution solution reagent strip preparation device according to the present invention.

[0037] Explanation of the labels in the diagram:

[0038] 1. Mixing tank; 101. Outer shell; 102. Inner liner; 103. Insulation cavity; 2. Support frame; 3. Coating assembly; 301. Guide frame; 302. Electric push rod; 303. Lifting plate; 304. Slide groove; 305. Sealing plate; 306. L-shaped connecting plate; 307. Sealing plate; 308. Coating roller; 3081. Central shaft; 3082. Liquid absorption layer; 3083. Anti-permeability layer; 3084. Soft rubber seat; 3085. Support rod; 3086. E-shaped tube; 3087. Movable rod; 3088. Spring; 3089. Piston; 4. First side plate; 5. 1. Collection box; 6. Drying oven; 7. Cleaning assembly; 701. Cleaning box; 702. Connecting shaft; 703. Gear; 704. Drive shaft; 705. Cleaning roller brush; 8. Unwinder; 9. Second side plate; 10. Winding machine; 11. Cutting machine; 12. L-shaped square tube; 13. Vacuum pump; 14. Low temperature heater; 16. Stirrer; 17. First rotating shaft; 18. Support platform; 19. Cutting table; 20. Cutting opening; 21. Second rotating shaft; 22. Inlet and outlet; 23. Support roller; 24. Drain valve; 25. Mounting groove; 26. Arc groove; 27. Discharge pipe. Detailed Implementation

[0039] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0040] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0041] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection or setting, a detachable connection or setting, or an integral connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0042] See Figures 1-10This invention provides a device for preparing europium-labeled antibody dilution solution reagent strips, including a support frame 2. A mixing tank 1 is installed on one side of the top of the support frame 2, and a reagent strip is disposed inside the support frame 2. A discharge pipe 27 is fixed to the bottom of the mixing tank 1. The mixing tank 1 is composed of an outer shell 101 and an inner liner 102. A stirrer 16 is installed in the middle of the mixing tank 1. The discharge pipe 27 is fixed to the middle of the bottom of the inner liner 102 and extends out of the outer shell 101. The gap between the outer side of the inner liner 102 and the inner side of the outer shell 101 is designed as a heat preservation cavity 103. An application component 3 is provided at the bottom of the discharge pipe 27. The application component 3 includes a guide frame 301 fixed to the bottom of the discharge pipe 27, and both ends of the guide frame 301 are provided with grooves 304. The inner walls of the grooves 304 are all... The guide frame 301 has two sliding blocks, one end of which is fixed with a sealing plate 305 that fits against the inner wall of both ends of the guide frame 301. A coating roller 308 is rotatably connected to one bottom side of each sealing plate 305. A lifting plate 303 is fixed to the other end of one of the sliding blocks. An electric push rod 302 for adjusting the height of the lifting plate 303 is installed on the outer wall of one end of the guide frame 301. The coating roller 308 includes a central shaft 3081, and the outer wall of the central shaft 3081 is provided with an anti-permeability layer 3083. The outer wall of the anti-permeability layer 3083 is provided with a liquid-absorbing layer 3082. Soft rubber seats 3084 are evenly distributed on the inner wall of the anti-permeability layer 3083 and the outer wall of the central shaft 3081. Multiple rows of evenly distributed E-shaped tubes 3086 are fixed to the outer wall of the central shaft 3081. Furthermore, the positions of the E-shaped tube 3086 and the soft rubber seat 3084 are staggered. The E-shaped tube 3086 is filled with inert gas. Movable rods 3087 are inserted into the three inner walls of the E-shaped tube 3086. A support rod 3085 is fixed to one end of each movable rod 3087, and a piston 3089 is fixed to one end of the outer wall of each movable rod 3087. A spring 3088 is installed at the middle position of the other end of the movable rod 3087 and the middle position of the inner wall of the E-shaped tube 3086. A liquid inlet hole communicating with the discharge pipe 27 is opened at the top center of the guide frame 301. An L-shaped connecting plate 306 is fixed to one side of each of the two sealing plates 305. A sealing plate 307 that fits against the bottom of the liquid inlet hole is fixed to the top of each L-shaped connecting plate 306. Using the aforementioned applicator roller 308... The height of the lifting plate 303 is adjusted by the electric push rod 302 in the application assembly 3, lowering the position of the sealing plate 305 and the application roller 308. This allows the application roller 308 to press the reagent strip into the arc-shaped groove 26. As the reagent strip moves, the application roller 308 rotates. At this time, the sealing plate 307 on one side of the sealing plate 305 moves away from the liquid inlet, and the reagent enters the guide frame 301. The liquid absorption layer 3082 of the application roller 308 absorbs the reagent in the guide frame 301. Due to the action of the soft rubber seat 3084, the E-shaped tube 3086, the movable rod 3087, the support rod 3085, etc., the application roller 308 can adhere well to the surface of the reagent strip. Since the E-shaped tube 3086 is filled with inert gas, the gas pressure inside the E-shaped tube 3086 remains stable.When one of the movable rods 3087 is compressed, the other movable rods 3087 will move in the opposite direction under air pressure. This compresses the liquid-absorbing layer 3082, allowing it to better contact the reagent strip and thus enabling the reagent to enter the strip more effectively.

[0043] In this invention, a drying chamber 6 and a cleaning component 7 are sequentially installed on the top of the support frame 2 near the middle position. First side plates 4 are fixed to both ends of one side of the drying chamber 6, and second side plates 9 are fixed to both ends of one side of the cleaning component 7 and the top of the support frame 2. A first rotating shaft 17 is rotatably connected to one end of each of the two first side plates 4, and a second rotating shaft 21 is rotatably connected to the bottom of one end of each of the two second side plates 9. Mounting grooves 25 are provided on both sides of the top of each of the two second side plates 9. An unwinding machine 8 and a winding machine 10 are respectively installed on the inner walls of the mounting grooves 25. The reagent strip is wound around the unwinding machine 8, the winding machine 10, the first rotating shaft 17, and the second rotating shaft 21. Figure 1 , Figure 2 and Figure 3 As shown, the arrangement of components such as the unwinding machine 8, the winding machine 10, the first rotating shaft 17, and the second rotating shaft 21 enables the reagent strips to be smoothly transported within the preparation device, making the entire preparation process continuous and efficient.

[0044] In this invention, a support platform 18 is fixedly installed on one side of each of the two first side plates 4, and the support platform 18 is located directly below the guide frame 301. An arc-shaped groove 26 is formed at the center of the top of the support platform 18, and the arc-shaped groove 26 is located directly below the coating roller 308. A collection box 5 is provided inside the support frame 2, and the collection box 5 is located below the first side plates 4 and the support platform 18. Figure 1 and Figure 5 As shown, the aforementioned arc-shaped groove 26 is used so that when the position of the applicator roller 308 is lowered, the reagent strip can be driven to synchronously adhere to the arc-shaped groove 26, increasing the contact area between the reagent strip and the applicator roller 308. This improves the applicator area and efficiency on the one hand, and increases the friction between the reagent strip and the applicator roller 308 on the other hand, making it easier to drive the applicator roller 308 to rotate. The reagent dripping during the preparation process is collected by the collection box 5, keeping the inside of the device clean.

[0045] In this invention, the cleaning assembly 7 includes a cleaning box 701 installed on one side of the drying oven 6 and the top of the support frame 2. Multiple support rollers 23 are rotatably connected to the inner wall of the cleaning box 701. A reagent strip is wound around the support rollers 23. Inlet and outlet ports 22 are provided on both sides of the cleaning box 701 and on the top and bottom of both sides of the drying oven 6. The reagent strip passes through the inlet and outlet ports 22. A drain pipe is fixed to the bottom of one side of the cleaning box 701, and a drain valve 24 is installed at one end of the drain pipe. Two mounting holes are provided on the side of the cleaning box 701 near the center. The inner walls of the mounting holes are rotatably connected to a drive shaft 704 of a connecting shaft 702 via sealed bearings. One end of the drive shaft 704 is fixedly connected to one end of one of the support rollers 23. A cleaning roller brush 705 is fixedly installed on one end of the connecting shaft 702, and the cleaning roller brush 705 adheres to the reagent strip. Gears 703 are installed on the other ends of the connecting shaft 702 and the drive shaft 704. The two gears 703 mesh with each other. Figure 2 and Figure 4 As shown, when the reagent strip enters the cleaning box 701 using the cleaning component 7 described above, multiple support rollers 23 inside the cleaning box 701 rotate in coordination with the reagent strip. At this time, through the meshing action of the gears 703 on the connecting shaft 702 and the transmission shaft 704, the cleaning roller brush 705 rotates as the reagent strip moves, thereby cleaning the impurities on the surface of the reagent strip and preventing impurities from affecting the application effect and subsequent test results.

[0046] In this invention, a vacuum pump 13 and a low-temperature heater 14 are respectively installed on one side of the drying oven 6, and an L-shaped square tube 12 is installed on the top side of the drying oven 6 and one side of the outer shell 101. The drying oven 6, the L-shaped square tube 12, and the heat preservation cavity 103 are connected. Figure 1 , Figure 2 , Figure 3 and Figure 6 As shown, the vacuum pump 13 and the low-temperature heater 14 described above are used. The vacuum pump 13 extracts the air from the drying chamber 6 to form a vacuum environment, which lowers the boiling point of water and accelerates the drying speed. At the same time, the low-temperature heater 14 provides an appropriate temperature to further promote the drying of the reagent strips. Under the action of the L-shaped square tube 12, the drying chamber 6 and the heat preservation chamber 103 are connected, making reasonable use of the heat generated during the drying process to heat the inside of the mixing tank 1, improving the mixing effect and energy utilization efficiency, and helping to maintain the temperature stability of the reagents in the mixing tank 1.

[0047] In this invention, a cutting machine 11 is installed on the top side of the two second side plates 9 near the winding machine 10, and a cutting table 19 is installed on the bottom side of one side of the two second side plates 9. A cutting opening 20 is provided in the middle of the cutting table 19, and the cutting table 19 is located directly below the cutting machine 11. Figure 1 , Figure 2 ,and Figure 5As shown, by using the above-mentioned cutting machine 11 and cutting table 19, the dried reagent strips can be transferred to the cutting machine 11, and the reagent strips can be cut according to the specifications set by the cutting machine 11 to obtain europium-labeled antibody dilution solution reagent strip products that meet the requirements.

[0048] A method for preparing europium-labeled antibody dilution solution reagent strips, applied to an apparatus for preparing europium-labeled antibody dilution solution reagent strips, includes the following steps:

[0049] Step 1: Prepare the europium-labeled antibody dilution solution according to the following proportions: Tris-HCl buffer, pH 7.2-7.6, concentration 20-50 mmol / L; bovine serum albumin, mass fraction 0.5%-2%; trehalose, mass fraction 1%-5%; ProClin 300 preservative, volume fraction 0.05%-0.2%; the remainder is deionized water.

[0050] Step 2: Preparation of the carrier: Nitrocellulose membrane is selected as the carrier for the reagent strip;

[0051] Step 3: Dilution solution: Inject all components of the prepared europium-labeled antibody dilution solution into mixing vessel 1, and mix thoroughly for 20-30 minutes using stirrer 16 in mixing vessel 1;

[0052] Step 4, even coating: Turn off the stirrer 16, wrap the reagent strip around the entire preparation device, clean the surface of the reagent strip with the cleaning component 7, and after drying, pass it from the bottom of the mixing tank 1 and evenly coat the europium-labeled antibody reagent onto the reagent strip with the coating component 3.

[0053] Step 5: Continue drying: Pass the applied reagent strip through the drying oven 6 and dry at a temperature of 45-60℃.

[0054] Step 6, Cutting and Packaging: Cut the dried reagent strips to the required length using the cutting machine 11. Place the cut reagent strips into a sealed bag, add desiccant, and vacuum pack.

[0055] In summary, the working principle of this invention is as follows: each component is injected into the mixing tank 1, and the europium-labeled antibody dilution solution reagent in the mixing tank 1 is stirred by the stirrer 16 to ensure that the reagent is fully and evenly mixed. The evenly mixed reagent flows to the coating component 3 through the discharge pipe 27.

[0056] The unwinding machine 8 releases the reagent strip without reagent coating. The reagent strip passes through the cleaning component 7, the drying chamber 6, and the first rotating shaft 17 in sequence, then returns to the drying chamber 6. After passing through the second rotating shaft 21, it is finally wound up by the winding machine 10. When the reagent strip enters the cleaning chamber 701, multiple support rollers 23 in the cleaning chamber 701 rotate in coordination with the reagent strip. At this time, through the meshing of the gears 703 on the connecting shaft 702 and the transmission shaft 704, the cleaning roller brush 705 rotates as the reagent strip moves, thereby cleaning the impurities on the surface of the reagent strip. The wastewater generated during cleaning is discharged through the drain pipe and drain valve 24. The cleaned reagent strip enters the drying chamber 6. The vacuum pump 13 extracts the air in the drying chamber 6 to form a vacuum environment, which lowers the boiling point of water and accelerates the drying speed. At the same time, the low temperature heater 14 provides an appropriate temperature to further promote the drying of the reagent strip. Moreover, the drying chamber 6, the L-shaped square tube 12, and the heat preservation chamber 103 are connected, so the heat generated during the drying process can be used reasonably to maintain the temperature stability of the reagent in the mixing tank 1 and accelerate the mixing efficiency.

[0057] During application, the electric push rod 302 pushes the lifting plate 303 down, causing the connected slider, sealing plate 305, and application roller 308 to descend. This allows the application roller 308 to press the reagent strip into the arc groove 26. As the reagent strip moves, the application roller 308 rotates. At this time, the sealing plate 307 on one side of the sealing plate 305 leaves the liquid inlet, and the reagent enters the guide frame 301. The liquid absorption layer 3082 of the application roller 308 absorbs the reagent in the guide frame 301. Due to the action of the soft rubber seat 3084, E-shaped tube 3086, movable rod 3087, support rod 3085, etc., the application roller 308 can fit well against the surface of the reagent strip. As the reagent strip moves, the liquid absorption layer 3082 evenly applies the reagent to the reagent strip. After application, the electric push rod 302 drives the lifting plate 303 to rise, and the sealing plate 307 re-blocks the liquid inlet to prevent the reagent from continuing to flow out.

[0058] After application, the reagent strip returns to the drying chamber 6. The dripping reagent falls into the collection box 5 inside the support frame 2 and is dried again in the drying chamber 6, allowing the reagent to penetrate the reagent strip better. Then, the dried reagent strip is transferred to the cutting machine 11, which cuts the reagent strip according to the set specifications, finally obtaining the europium-labeled antibody dilution solution reagent strip product that meets the requirements.

[0059] In light of current practical needs, the above-described embodiments adopted in this application are not limited to these. Any changes made within the scope of knowledge possessed by those skilled in the art without departing from the concept of this application still fall within the protection scope of this invention.

Claims

1. A device for preparing europium-labeled antibody dilution solution reagent strips, characterized in that: The device includes a support frame, a mixing tank mounted on the top side of the support frame, and a reagent strip disposed within the support frame. A discharge pipe is fixed to the bottom of the mixing tank, and an application assembly is disposed at the bottom of the discharge pipe. The application assembly includes a guide frame fixed to the bottom of the discharge pipe, with grooves at both ends of the guide frame. Slider blocks slide along the inner walls of the grooves. Sealing plates are fixed to one end of each slider and adhere to the inner walls of both ends of the guide frame. Application rollers are rotatably connected to the bottom of one side of each sealing plate. A lifting plate is fixed to the other end of one of the sliders. An electric push rod for adjusting the height of the lifting plate is mounted on the outer wall of one end of the guide frame. The application roller includes a central shaft, with an anti-permeability layer on its outer wall and a liquid-absorbing layer on its outer wall. Soft rubber seats are evenly distributed on the inner wall of the anti-permeability layer and the outer wall of the central shaft. Multiple rows of evenly distributed E-shaped tubes are fixed to the outer wall of the central shaft, and the positions of the E-shaped tubes and the soft rubber... The seats are staggered. The E-shaped tube is filled with inert gas. Movable rods are inserted into the three inner walls of the E-shaped tube. A support rod is fixed to one end of each movable rod. A piston is fixed to one end of each movable rod on its outer wall. A spring is installed at the middle of the other end of the movable rod and the inner wall of the E-shaped tube. A liquid inlet is opened at the top center of the guide frame, which communicates with the discharge pipe. An L-shaped connecting plate is fixed to one side of each of the two sealing plates. A sealing plate that fits against the bottom of the liquid inlet is fixed to the top of each L-shaped connecting plate. A drying chamber and a cleaning component are installed sequentially on the top of the support frame near the middle position. A first side plate is fixed to both ends of one side of the drying chamber. A support platform is fixed to one side of each of the two first side plates. The support platform is located directly below the guide frame. An arc-shaped groove is opened at the top center of the support platform. The arc-shaped groove is located directly below the coating roller. A collection box is installed inside the support frame. The collection box is located below the first side plate and the support platform.

2. The apparatus for preparing europium-labeled antibody dilution solution reagent strips according to claim 1, characterized in that: The mixing tank is composed of an outer shell and an inner liner. An agitator is installed in the middle of the mixing tank. The discharge pipe is fixed at the bottom middle of the inner liner and extends out of the outer shell. The gap between the outer side of the inner liner and the inner side of the outer shell is designed as an insulation cavity.

3. The apparatus for preparing europium-labeled antibody dilution solution reagent strips according to claim 2, characterized in that: The cleaning component has two ends on one side and the top of the support frame with a second side plate. One end of the two first side plates is rotatably connected to a first rotating shaft, and the bottom of one end of the two second side plates is rotatably connected to a second rotating shaft. The top two sides of the two second side plates are provided with mounting grooves. The inner walls of the mounting grooves are respectively installed with an unwinding machine and a winding machine. The reagent strip is wound on the unwinding machine, the winding machine, the first rotating shaft and the second rotating shaft.

4. The apparatus for preparing europium-labeled antibody dilution solution reagent strips according to claim 3, characterized in that: The cleaning assembly includes a cleaning box installed on one side of the drying oven and the top of the support frame. Multiple support rollers are rotatably connected to the inner wall of the cleaning box. The reagent strip is wound around the support rollers. Inlet and outlet are provided on both sides of the cleaning box and on the top and bottom of both sides of the drying oven. The reagent strip passes through the inlet and outlet. A drain pipe is fixed to the bottom of one side of the cleaning box, and a drain valve is installed at one end of the drain pipe.

5. The apparatus for preparing europium-labeled antibody dilution solution reagent strips according to claim 4, characterized in that: The cleaning box has two mounting holes on one side near the center. The inner walls of the mounting holes are rotatably connected to the drive shafts of the connecting shafts via sealed bearings. One end of the drive shaft is fixedly connected to one end of one of the support rollers. A cleaning roller brush is fixedly installed on one end of the connecting shaft and is attached to the reagent strip. Gears are installed on the other ends of the connecting shaft and the drive shaft, and the two gears mesh with each other.

6. The apparatus for preparing europium-labeled antibody dilution solution reagent strips according to claim 5, characterized in that: A vacuum pump and a low-temperature heater are installed on one side of the drying chamber, and an L-shaped square tube is installed on the top side of the drying chamber and the outer shell side. The drying chamber, the L-shaped square tube and the heat preservation cavity are connected.

7. The apparatus for preparing europium-labeled antibody dilution solution reagent strips according to claim 6, characterized in that: A cutting machine is installed on the top side of the two second side plates near the winding machine, and a cutting table is installed on the bottom side of one side of the two second side plates. A cutting opening is provided in the middle of the cutting table, and the cutting table is located directly below the cutting machine.

8. A method for preparing europium-labeled antibody dilution solution reagent strips, applied to the europium-labeled antibody dilution solution reagent strip preparation apparatus as described in claim 7, characterized in that: Includes the following steps: Step 1: Prepare the europium-labeled antibody dilution solution according to the following proportions: Tris-HCl buffer, pH 7.2-7.6, concentration 20-50 mmol / L; bovine serum albumin, mass fraction 0.5%-2%; trehalose, mass fraction 1%-5%; ProClin300 preservative, volume fraction 0.05%-0.2%; the remainder is deionized water. Step 2: Preparation of the carrier: Nitrocellulose membrane is selected as the carrier for the reagent strip; Step 3: Dilution solution: Inject all components of the prepared europium-labeled antibody dilution solution into the mixing vessel and mix thoroughly for 20-30 minutes using the stirrer inside the mixing vessel; Step 4: Evenly apply the reagent strip: Turn off the stirrer, wrap the reagent strip around the entire preparation device, clean the surface of the reagent strip using the cleaning component, and after drying, pass it from the bottom of the mixing tank and use the application component to evenly apply the europium-labeled antibody reagent onto the reagent strip. Step 5: Continue drying: Pass the applied reagent strip through the drying oven at a temperature of 45-60℃; Step 6, Cutting and Packaging: Cut the dried reagent strips to the required length using a cutting machine. Place the cut reagent strips in a sealed bag, add desiccant, and vacuum pack.

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