Composition, kit and detection method for detecting aspirin resistance related SNP (Single Nucleotide Polymorphism)

Abstract

The invention belongs to the technical field of aspirin-related gene polymorphism detection, and particularly relates to a composition, a kit and a detection method for detecting aspirin resistance-related SNP (Single Nucleotide Polymorphism), which comprises a primer probe combination for amplifying rs5918, rs12041331, rs6065 and rs730012 gene loci, and a primer probe combination for amplifying rs5918, rs12041331, rs6065 and rs730012 gene loci. The primer probe combinations of the four gene loci respectively comprise a wild type upstream primer, a wild type downstream primer, a mutant type upstream primer, a mutant type downstream primer, a wild type probe and a mutant type probe, and the detection method comprises the following steps: mixing the primer probe combinations for amplifying the rs5918 gene locus, the rs12041331 gene locus, the rs6065 gene locus and the rs730012 gene locus, carrying out multiple amplification, and purifying the product after the amplification is finished, so as to obtain the rs5918 gene locus, the rs12041331 gene locus, the rs6065 gene locus and the rs730012 gene locus. According to the present invention, the specific primers and the probe are designed to combine with the microarray chip, and the genotype threshold is constructed, such that the high-sensitivity and high-specificity detection of the aspirin-related SNP site is achieved so as to guide the aspirin individualized medication.

Images