Separation method and application of exopolysaccharides in fermented grains
CN120248154APending Publication Date: 2025-07-04KWEICHOW MOUTAI COMPANY
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Patent Information
- Application Number
- CN202510333460.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-20
- Publication Date
- 2025-07-04
AI Technical Summary
Technical Problem
The prior art is difficult to efficiently separate mixed symbiotic microbial extracellular polysaccharides in sauce-flavored liquor mash, and chemical methods may destroy cells, physical methods are inefficient and impurities are severely disturbed.
Method used
Ultrasonic combined with differential centrifugation is used to optimize parameters to separate the extracellular polysaccharides in sauce-flavored liquor mash, shorten the extraction time to 1 hour, and avoid the use of chemical reagents.
Benefits of technology
The separation efficiency and concentration of extracellular polysaccharides are significantly improved, the extraction time is reduced, the separation efficiency is improved, and the polysaccharide purity is maintained.
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Abstract
The invention belongs to the technical field of wine brewing, and relates to a separation method and application of exopolysaccharides in fermented grains, and the separation method comprises the following steps: weighing a fermented grain sample, adding the fermented grain sample into pure water, and carrying out oscillation, ultrasonic treatment and centrifugal treatment to obtain a fermented grain leach liquor containing a mixed symbiotic microbial population; carrying out activation culture, centrifugation and washing on the mixed symbiotic microorganisms in the fermented grain leach liquor to obtain a test bacterium solution; performing ultrasonic treatment on the to-be-tested bacterial liquid, and then performing differential centrifugation and filtration to obtain a solution containing exopolysaccharides; the ultrasonic treatment time is 3.5-4.5 min, and the differential centrifugation mode is as follows: centrifuging for 5-15 min at the rotating speed of 400-600 g, collecting the supernatant, centrifuging for 5-15 min at the rotating speed of 4000-5000 g, collecting the supernatant, centrifuging for 5-15 min at the rotating speed of 9000-11000 g, and centrifuging for 5-15 min at the rotating speed of 9000-11000 g. The extracellular polysaccharide in the fermented grains is separated by combining ultrasound with differential centrifugation, and meanwhile, the parameters of ultrasound and differential centrifugation are optimized, so that the separation efficiency and concentration of the extracellular polysaccharide in the fermented grains are improved.
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