A fusarium solani and use thereof

Abstract

The present application belongs to the field of microbial technology, and particularly relates to a Fusarium solani and application thereof. The present application obtains a Fusarium solani (Fusarium solani) Jing-YSD-PDA2 by adopting tissue block separation method and streak separation method from fresh Chufa, and the Fusarium solani has good IAA production capacity and nitrogen fixation activity. In addition, the strain has certain inhibitory effect on Rhizomucor pusillus and Fusarium decemcellulare, and has strong inhibitory effect on Fusarium oxysporum, and the inhibition rate is 61.00%, and the biocontrol potential is good, and the Fusarium solani can provide new strain resources for the selection of biocontrol agents for Fusarium oxysporum and stem rot.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a Fusarium solani and its applications. Background Technology

[0002] Tiger nuts (Cyperus esculentus L.), also known as oil nut grass, are perennial herbaceous plants belonging to the genus Cyperus in the family Cyperaceae. They are native to the Mediterranean region, North Africa, and the Nile River basin. In my country, the main cultivation areas are concentrated in southern Xinjiang, Ordos in Inner Mongolia, and Jilin (Sun Rui, Zhong Peng, Liu Zedong, et al. The utilization value of tiger nuts and its industrial development status [J]. Feed Industry, 2023, 44(21):19-24.). Tiger nuts have a protein content similar to that of oats (10%–13%), with an albumin content as high as 80%–95%. The oil in tiger nuts is mainly oleic acid, with an oleic acid content of 65%–75% (Duan Shuai, Zhang Dejian, Yao Yujun, et al. Research progress on nutritional value and processing application of tiger nuts [J]. Food Science and Technology, 2022(007):047.). The fat content of tiger nuts is 18.7%–26.7%, the soluble sugar content is 15%–25%, the dietary fiber content is 6.7%–11.0%, and the starch content is 25%–40%, of which the amylose content is 13.9% and the amylopectin content is 77.4% (Chen Lina, Shi Mao. Study on determination of amylose and amylopectin content of tiger nuts by dual wavelength method [J]. Food Science and Technology, 2010(8):3). It is a multi-purpose crop that integrates grain, oil and feed, and can be widely used in the preparation of food, biodiesel, feed, etc. Tiger nuts grow rapidly, have a well-developed root system, and strong tillering and regeneration ability. They are less susceptible to diseases throughout their growth period and do not require prevention and control. They are drought-resistant, flood-resistant, tolerant of poor soil and saline-alkali soil, and have strong stress resistance and adaptability. They can be planted in low, medium and high fertility soils such as sandy soil, albic soil, saline-alkali soil, black soil and wasteland slopes (Sun Rui, Zhong Peng, Liu Zedong, et al. Utilization value of tiger nuts and its industrial development status [J]. Feed Industry, 2023, 44(21):19-24.). They are very suitable for planting on marginal lands such as desertified areas. They can contribute to the country's edible oil and food security without competing with staple food crops for land. They are a high-quality crop that can be used for saline-alkali land improvement and marginal land utilization, and play an important role in soil and water conservation, windbreak and sand fixation and protection of the natural ecological environment.

[0003] Currently, existing technologies have limited research on endophytic fungi within the resilient tiger nut, especially on their role in plant growth promotion and biological control. Oyedara et al. (Oyedara OO, Rufai AB, Okunlola GO, et al. Nutritious and Endophytic Composition of Edible Tubers of Tiger Nut (Cyperus esculentus L.)[J]. Jordan Journal of Biological Sciences, 2022, 15(4). DOI: 10.54319 / jjbs / 150408.) isolated two endophytic fungi from tiger nuts: Saccharomyces cerevisiae and Candida tropicalis. Wang Saisai et al. (Wang Saisai. Study on the growth-promoting effect of endophytic fungi in tiger nuts and the synthesis mechanism of flavonoids[D]. Jiangnan University, 2022.) studied the community diversity of different varieties of tiger nuts (Cyperus esculentus I and Cyperus esculentus II).

[0004] In conclusion, it is necessary to conduct in-depth research on the role of endophytic fungi in tiger nuts in plant growth promotion and biological control, in order to provide strain resources for the research of microbial agents in biological control and to provide a theoretical basis and scientific guidance for biological control in agricultural production.

[0005] Water chestnut (Eleocharis dulcis), also known as horse chestnut, belongs to the same family as tiger pea, both belonging to the Cyperaceae family. Water chestnuts are often susceptible to diseases during their growth, such as wilt and stem rot. Currently, traditional control methods for wilt and stem rot mainly include agricultural and chemical control. In recent years, traditional agricultural control methods have proven ineffective in fundamentally controlling the disease, and their effectiveness is inconsistent. Furthermore, long-term use of chemical pesticides can lead to drug resistance in pathogens, and excessive pesticide use can damage the soil, exacerbate pesticide pollution, and leave excessive toxic and harmful substances in crops. Therefore, biological control methods are receiving increasing attention. Plant endophytic fungi are a natural resource for the biological control of plant diseases. Most plants harbor a large number of endophytic fungi with antibacterial activity, which can be used to defend against pathogen attacks.

[0006] Water chestnuts and tiger nuts both belong to the Cyperaceae family and share certain similarities in plant morphology, physiological characteristics, and growth habits. This means they may have similar susceptibility to certain pathogens or similar defense mechanisms against diseases. Therefore, endophytic fungi isolated from tiger nuts hold promise for the control of water chestnut stem rot. Summary of the Invention

[0007] In order to overcome the shortcomings and disadvantages of the prior art, the primary objective of this invention is to provide a Fusarium solani.

[0008] Another object of the present invention is to provide the application of the above-mentioned Fusarium solani.

[0009] Another object of the present invention is to provide a microbial fertilizer.

[0010] The fourth object of the present invention is to provide an antibacterial agent.

[0011] The objective of this invention is achieved through the following technical solution:

[0012] A Fusarium solani fungus, named Fusarium solani-YSD-PDA2, was deposited on July 11, 2024, at the Guangdong Provincial Center for Microbial Culture Collection, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 64860.

[0013] The aforementioned Fusarium solani was isolated from tiger nuts and is an endophytic fungus;

[0014] The aforementioned Fusarium solani has the following morphological characteristics:

[0015] On PDA medium, the colonies of strain Jing-YSD-PDA2 are white, nearly round, with fluffy hyphae and transverse septa that divide the hyphae into several segments.

[0016] The application of the aforementioned Fusarium solani and / or its fermentation broth in the fields of plant growth promotion or soil remediation;

[0017] The application of Fusarium solani and / or its fermentation broth in the preparation of indoleacetic acid;

[0018] The application of Fusarium solani and / or its fermentation broth in the preparation of microbial fertilizers;

[0019] The application of Fusarium solani and / or its fermentation broth in inhibiting plant pathogens, preventing and controlling plant diseases caused by plant pathogens, or in the preparation of plant pathogen inhibitors or biocontrol agents;

[0020] The plant pathogens mentioned are at least one of Mucor rouxianus, Curvularialunata, and Fusarium commune;

[0021] A plant growth promoter comprising the above-mentioned Fusarium solani and / or its fermentation broth;

[0022] A soil remediation agent comprising the aforementioned Fusarium solani and / or its fermentation broth;

[0023] A microbial fertilizer comprising the aforementioned Fusarium solani and / or its fermentation broth;

[0024] An antibacterial agent comprising the above-mentioned Fusarium solani and / or its fermentation broth;

[0025] The present invention has the following advantages and effects compared with the prior art:

[0026] (1) In this invention, an endophytic fungus, Jing-YSD-PDA2, was isolated and purified from tiger nuts using tissue block separation and streak plating methods. Through morphological and molecular identification, the endophytic fungus of tiger nuts was identified as Fusarium solani.

[0027] (2) The Fusarium solani-YSD-PDA2 provided by the present invention has a good IAA production capacity, and the mass concentration of IAA produced is 0.52 mg / L.

[0028] (2) The Fusarium solani provided by this invention, YSD-PDA2, was inoculated onto Assumption medium and cultured for three generations. It can still grow normally and in good condition, indicating that it has nitrogen-fixing activity.

[0029] (3) The Fusarium solani-YSD-PDA2 provided by this invention has a certain inhibitory effect on Mucor roximately and Curvularia crescentis, the pathogen of fusarium rot in horseshoes; it has strong resistance to Fusarium oxysporum, the pathogen of fusarium wilt in horseshoes, with an inhibition rate of 61.00%. It has good biocontrol potential and can provide new strain resources for the selection of biocontrol agents for fusarium wilt and stem rot in horseshoes.

[0030] (4) The Fusarium solani-YSD-PDA2 provided by this invention has the ability to produce indoleacetic acid (IAA) and fix nitrogen, thereby helping plants to fully absorb and utilize the nutrients they need, promoting plant growth, and has the potential to be developed into a microbial fertilizer. Attached Figure Description

[0031] Figure 1 This is a colony morphology diagram of strain Jing-YSD-PDA2.

[0032] Figure 2 This is a microscopic image of strain Jing-YSD-PDA2.

[0033] Figure 3This is a gel electrophoresis result of the 18S rDNA PCR amplification product of strain Jing-YSD-PDA2.

[0034] Figure 4 This is a phylogenetic tree diagram constructed based on the 18S rDNA sequence of strain Jing-YSD-PDA2.

[0035] Figure 5 This is a graph showing the qualitative analysis results of the IAA production capacity of strain Jing-YSD-PDA2.

[0036] Figure 6 These are the PC standard curve (low concentration standard curve) and the S2 standard curve (high concentration standard curve) graphs.

[0037] Figure 7 This is a diagram showing the nitrogen fixation ability of strain Jing-YSD-PDA2 in Assumption medium (3rd generation).

[0038] Figure 8 This is a diagram showing the antagonistic effect of strain Jing-YSD-PDA2 on Mucor leucocephala. In the diagram, A is the control; B is the treatment (Mucor leucocephala on the left and Jing-YSD-PDA2 on the right).

[0039] Figure 9 This is a diagram showing the antagonistic effect of strain Jing-YSD-PDA2 against the pathogen of horseshoe stem rot (Curvularia crescentis). In the diagram, A is the control and B is the treatment (strain Jing-YSD-PDA2 is on the left and the pathogen of wilt is on the right).

[0040] Figure 10 This is a diagram showing the antagonistic effect of strain Jing-YSD-PDA2 against the pathogen of fusarium wilt in horseshoes (Fusarium oxysporum). In the diagram, A is the control and B is the treatment (strain Jing-YSD-PDA2 is on the left and the pathogen of fusarium wilt is on the right). Detailed Implementation

[0041] The present invention will be further described in detail below with reference to the embodiments and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0042] 1. Materials and reagents involved in the embodiments

[0043] (1) Test pathogens: Curvularia lunata, strain number: 142975, provided by Beina Chuanglian Biotechnology Co., Ltd.; Mucor rouxianus, strain number: AS3.3447, provided by Shanghai Center for Biological Preservation; Fusarium commune, provided by China Center for Type Culture Collection, strain number: CCTCC AF 2013028.

[0044] (2) Tiger nut tubers: Tiger nut tubers were collected from 20 vigorous, healthy, and disease-free tiger nut plants in Xinshao Town, Shaoguan City, Guangdong Province (N24°46', E113°39', altitude 105m).

[0045] (3) Test culture medium: Potato dextrose agar (PDA) medium: 5.0g potato extract powder, 20.0g glucose, 15.0g agar, 1L distilled water, heated to boiling until completely dissolved, autoclaved at 121℃ for 15min, cooled to about 55℃, added kanamycin base and ampicillin, mixed thoroughly and poured into plates for later use in the culture of plant pathogenic fungi and the determination of antagonistic effects of strains.

[0046] Table 1 Main Reagents List

[0047]

[0048] 2. Data Statistics and Processing in the Examples

[0049] Each experiment was performed in triplicate. Data was collected and analyzed using Excel, and graphs were generated using MEGA 11.0 software.

[0050] Example 1: Isolation and purification of strains

[0051] 1. Experimental Methods

[0052] 1.1 Sample preparation

[0053] (1) Sample pretreatment: The collected tiger nut tuber samples were placed in a labeled resealable bag and stored at 4°C before being brought back to the laboratory. The surface mud and sand were removed by rinsing with tap water for 5 minutes.

[0054] (2) Surface disinfection and detection of the strain: The method of Qu Siqi (Qu Siqi. Study on the effect of endophytic fermentation on saponin content of Northeast ginseng [D]. Yanbian University, 2020.) was followed. Surface disinfection treatment: soaking in 75% ethanol solution for 2 min, rinsing 5 times with sterile water, soaking in 2% NaClO solution for 30 s, rinsing 5 times with sterile water, repeating 3-4 times, and then blotting dry with sterile filter paper. Take 0.1 mL of sterile water from the last rinse and spread it on PDA medium, incubate upside down at 28℃ for 5-7 days, and detect the surface sterilization effect to ensure complete surface disinfection and that the isolated strain is an endophytic fungus rather than an exogenous contaminant.

[0055] 1.2 Isolation and purification of endophytic fungi

[0056] The method was followed according to Zang Sanli (Zang Sanli. Isolation, Screening and Characterization of Endophytic Bacteria from Limonene-Producing Plants [D]. Taiyuan: Shanxi University, 2019.). After surface sterilization, the samples were aseptically cut into 1cm × 1cm pieces with a sterile blade and inoculated onto PDA solid medium plates. Three replicates were made for each sample, and the plates were incubated upside down at 28℃. After bacterial growth around the sample, a small amount of hyphae was picked from the edge using an inoculation loop and transferred to a fresh PDA medium. After the hyphae matured, the outer edge hyphae were picked using the inoculation loop, and this process was repeated until a single strain was obtained. The strain was then transferred to an agar slant and stored at 4℃. 20 μg / mL kanamycin and 20 μg / mL ampicillin were added to the PDA medium to inhibit bacterial growth.

[0057] 1.3 Identification of Endophytic Fungi

[0058] (1) Morphological identification of endophytic fungi:

[0059] The strain isolated and purified from tiger nut tubers was inoculated onto PDA plates and cultured at 28°C. Colony characteristics were observed. Hyphae (including sporophytes) of the purified strain were selected, prepared into slides, and their morphology, sporulation structures, and spore morphology were observed under a microscope. The strain was preliminarily identified using a fungal taxonomy manual. Based on the culture characteristics, the strain was identified to a genus, and then species identification was performed according to the classification criteria of different genera.

[0060] (2) Identification of the ITS gene sequence of the strain:

[0061] Genomic DNA was extracted from the isolated strain according to the Ezup column-based fungal genomic DNA extraction kit instructions. Using this DNA as a template, PCR amplification was performed using ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4-R (5'-TCCTCCGCTTATTGATATGC-3') as primers. The PCR amplification system consisted of 25.0 μL, including 1.0 μL template DNA, 12.5 μL 50 mM MgSO4, 1 μL each of 10 μmol / L primers ITS1 and ITS4, and 9.5 μL ddH2O. The PCR reaction conditions were: pre-denaturation at 95℃ for 5 min; denaturation at 94℃ for 30 s, annealing at 57℃ for 30 s, extension at 72℃ for 90 s; repeated 30 cycles from denaturation to extension; final extension at 72℃ for 10 min; and finally, storage at 4℃. After detecting the PCR products by agarose gel electrophoresis, the samples were sent to Shanghai Sangon Biotech Co., Ltd. for sequencing. The obtained sequences were submitted to the NCBI GenBank database for BLAST homology comparison analysis. A phylogenetic tree was constructed using MEGA11.0 with the neighbor-joining method to determine the taxonomic position of the strains.

[0062] Molecular weight comparison: The electrophoretic bands of the sample are compared with the electrophoretic bands of the marker to determine the molecular weight of the DNA of the sample strain.

[0063] 2. Results Analysis

[0064] 2.1 Isolation and Identification of Endophytic Fungi

[0065] One-year-old tiger nut tubers were isolated and purified using potato dextrose agar (PDA) medium to obtain an endophytic fungus, which was designated as Jing-YSD-PDA2.

[0066] 2.2 Morphological identification

[0067] The colony morphology of strain Jing-YSD-PDA2 was observed, and the cell and spore morphology were observed using an optical microscope. The results are shown in the figure. Figure 1 and Figure 2 On PDA medium, the colonies of strain Jing-YSD-PDA2 are white, nearly round, with fluffy hyphae and transverse septa that divide the hyphae into several segments.

[0068] 2.3 Molecular biological identification

[0069] The strain Jing-YSD-PDA2 was amplified using universal fungal primers, and the PCR product was detected by agarose gel electrophoresis, yielding a 1323bp fragment. Figure 3 The PCR products were sequenced, and the sequencing results are shown below:

[0070] Strain Jing-YSD-PDA2 (1323bp):

[0071]

[0072] The above sequences were submitted to the NCBI GenBank database for BLAST homology alignment analysis. After downloading similar sequences, a phylogenetic tree was constructed using the neighbor-joining method with MEGA 11.0 software. The results are shown in [Figure number missing]. Figure 4 The strain Jing-YSD-PDA2 clustered on one branch with Fusarium solani (ON950402.1) and Fusarium solani (ON026100.1), and the similarity rate between strain Jing-YSD-PDA2 and Fusarium solani was 99.62%, indicating that they were closely related.

[0073] Based on morphological characteristics, strain Jing-YSD-PDA2 was identified as Fusarium solani and named Fusarium solani Jing-YSD-PDA2. It was deposited on July 11, 2024, at the Guangdong Provincial Center for Microbial Culture Collection, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCCNo: 64860.

[0074] Example 2: Determination of IAA production and nitrogen fixation capacity of the strain

[0075] 1. Experimental Methods

[0076] 1.1 IAA Production Capacity Determination

[0077] (1) Preparation of PC colorimetric solution: Weigh 12g of FeCl3 and dissolve it in 300mL of distilled water, then slowly add 429.7mL of 98% H2SO4. After cooling, make up to 1L. The determination range is 0.3~20mg / L.

[0078] Preparation of S2 colorimetric solution: Dissolve 4.5g FeCl3 in 300mL distilled water, then slowly add 587.4mL of 98% H2SO4. After cooling, bring the volume to 1L. The IAA range is 5-200mg / L.

[0079] (2) Qualitative analysis: The Salkowski method was used (Arun K. Screening and identification of abiotic stress-responsive efficient antifungal Pseudomonas spp. from ricerhizospheric soil.[J.]).

[0080] Biotechnologia, 2021, 102(1):15-19.), inoculated into King's liquid medium, 3 replicates, cultured at 28℃ and 120 r / min for 5 days with shaking, centrifuged at 8000 r / min for 10 min, and the supernatant was collected into test tubes. 2 mL of PC colorimetric solution and S2 colorimetric solution were added respectively, and the tubes were incubated in the dark at room temperature for 30 min to observe the color change. If the color turns red, it indicates the production of IAA, and the darker the color, the greater the amount of IAA produced. If the color does not change, no IAA is produced.

[0081] (3) Quantitative analysis: Two sets of IAA standard curves were plotted using standard samples for quantitative testing. The concentration gradient of the first set was 2.5, 5.0, 7.5, 10.0, 12.5, and 15.0 mg / L, and the concentration gradient of the second set was 25, 50, 75, 100, 125, and 150 mg / L. Following the above colorimetric method, the absorbance was measured at 530 nm using a UV spectrophotometer. A standard curve was plotted, and the equation of the standard curve was calculated. The OD value of the chromogenic bacterial solution from (2) was measured at 530 nm using a UV spectrophotometer. PC colorimetric solution and S2 colorimetric solution were added to distilled water as controls. The absorbance OD value of each bacterial solution was measured at 530 nm. 530 The amount of IAA in a unit volume of culture medium was calculated according to the standard curve.

[0082] 1.2 Nitrogen fixation capacity determination

[0083] Nitrogen fixation capacity was tested using nitrogen-free medium (Cao Ziqi, Zhao Xiaoqing, Zhang Xiangqian, et al. Effects of nitrogen application level on nitrogen, phosphorus and potassium accumulation, distribution and yield of tiger pea in sandy soil [J / OL]. Acta Agronomica Sinica, 1-14 [2024-04-22].). The activated test strain was inoculated on Ashby medium and cultured at 28℃ for 7 days. The culture was repeated three times. If the strain grew normally on Ashby medium, it indicated that the strain had nitrogen fixation activity.

[0084] 2. Results Analysis

[0085] 2.1 Results of IAA Production Capacity Measurement

[0086] The IAA production capacity of the endophytic fungal strain Jing-YSD-PDA2 isolated from tiger nuts was determined by Salkowski method. The test tubes containing Jing-YSD-PDA2 turned light pink, indicating that this endophytic fungus has a good IAA production capacity. Figure 5 ).

[0087] After testing the IAA standard, the absorbance was measured at a wavelength of 530 nm using a microplate reader. The obtained data were then used to plot standard curve equations. The PC standard curve equation is y = 0.0312x + 0.0145, and the S2 standard curve equation is y = 0.0094x + 0.2171. Figure 6 ), R 2 The values ​​were 0.9931 and 0.9424, respectively, showing a good linear relationship. This was determined by measuring OD... 530 The absorbance of the test sample was measured at 530 nm using an ELISA reader, and the amount of IAA produced by the strain was calculated based on the standard curve equation. The concentration of IAA produced by strain Jing-YSD-PDA2 was calculated to be 0.52 mg / L.

[0088] Table 2. Qualitative and quantitative screening results of IAA secretion in strain Jing-YSD-PDA2

[0089]

[0090] 2.2 Results of nitrogen fixation capacity determination of strain Jing-YSD-PDA2

[0091] The strain YSD-PDA2 was inoculated onto Assumption medium and cultured. For the first generation, mycelia were picked using an inoculation loop and cultured on fresh plates. The same method was repeated for the second generation, and the culture was continued for three generations. Figure 7 Afterwards, it was found that Jing-YSD-PDA2 could grow normally and in good condition, indicating that it has nitrogen fixation activity.

[0092] Example 3

[0093] 1. Experimental Methods

[0094] The endophytic fungus Jing-YSD-PDA2 isolated and purified in Example 1 was screened using the plate confrontation method (Cui Y, Zhu Y, Dong G, et al. Evaluation of the control efficacy of antagonistic bacteria from V-Ti magnetite mine tailings on kiwifruit brown spots in pot and field experiments[J]. Frontiers in Microbiology, 2024, 151280333-1280333.). First, the endophytic fungus Jing-YSD-PDA2 and the pathogens (Curvularia lunata, strain number: 142975, Mucor rouxianus, strain number: AS3.3447; Fusarium commune, strain preservation number: CCTCC AF 2013028) of tiger nuts were inoculated on two PDA media and cultured. After suitable growth, a fungal cake was taken from each medium using a punch. Two mycelial discs were inoculated onto the same PDA medium, approximately 4 cm apart. Each treatment was repeated three times, with a single pathogenic mycelial disc used as a blank control. The media were incubated at 28°C for 3–5 days. The colony diameters of endophytic fungi, pathogenic bacteria, and the blank control pathogen were measured, and the inhibition rate was calculated.

[0095]

[0096] 2. Results Analysis

[0097] The antagonistic effects of strain Jing-YSD-PDA2 on *Mucor leucovora*, *Curvularia crescentis* (the pathogen causing rot of horseshoe stems), and *Fusarium oxysporum* (the pathogen causing wilt of horseshoe stems) were observed using the plate confrontation method. After 5 days of confrontation culture, the colony diameters of the control pathogen and the fungus targeted by the treated pathogen were measured, and the inhibition rate was calculated. Strain Jing-YSD-PDA2 showed certain inhibitory effects on *Mucor leucovora* and *Curvularia crescentis* (the pathogen causing rot of horseshoe stems) (Table 3). Figure 8-9 The strain exhibited good inhibitory effects against the pathogen causing fusarium wilt of horseshoe crabs. Specifically, strain Jing-YSD-PDA2 showed an inhibition rate of 61.00% against Fusarium oxysporum (Table 3). Figure 10 ).

[0098] Table 3. Inhibition rates of strain Jing-YSD-PDA2 against different pathogens.

[0099]

[0100] Note: "++" indicates a strong effect, "++" indicates a medium effect, "+" indicates a weak effect, and "-" indicates no antagonistic effect.

[0101] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A type of Fusarium solani ( Fusarium solani ) Respect-YSD-PDA2, characterized in that This strain was deposited on July 11, 2024, at the Guangdong Provincial Center for Microbial Culture Collection, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, with accession number GDMCC No: 64860.

2. The use of Fusarium solanie and / or its fermentation broth as described in claim 1 in the preparation of indoleacetic acid.

3. The application of Fusarium solani and / or its fermentation broth as described in claim 1 in the preparation of microbial fertilizers.

4. The use of Fusarium solani and / or its fermentation broth as described in claim 1 in inhibiting plant pathogens, preventing and controlling plant diseases caused by plant pathogens, or in the preparation of plant pathogen inhibitors or biocontrol agents; The plant pathogen mentioned is Mucor rumeni ( Mucor rouxianus ) and Curvularia crescentis ( Curvularia lunata At least one of the following.

5. A microbial fertilizer, characterized in that... It contains Fusarium solani as described in claim 1 and / or its fermentation broth.

6. An antibacterial agent, characterized in that... It contains Fusarium solani as described in claim 1 and / or its fermentation broth.

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