Preparation device, preparation method and application of biogel repair patch

Abstract

The invention discloses a preparation device, a preparation method and application of a biogel repair patch, and aims to overcome the defects that currently common blood products are loose in gel structure, high in degradation speed, insufficient in mechanical strength, high in water content and incapable of meeting various requirements such as filling, suturing and wrapping in clinical operations. The preparation device comprises a preparation device body, a detachable tray is installed on the preparation device body, a water filtering film is loaded on the tray, water filtering holes are formed in the tray, a piston is installed in the preparation device body, a needle head can be inserted into the piston, and a forming cavity is formed between the piston and the water filtering film in the preparation device body. And the piston moves to increase the air pressure in the forming cavity so as to extrude water in the gel formed on the water filtering film out of the water filtering film. The preparation device facilitates preparation of the biogel repair patch, and the prepared biogel repair patch is compact in structure, good in mechanical strength and suitable for various application scenes such as filling supporting, suturing fixing and wound surface application.

Description

Technical Field

[0001] The present invention relates to the technical field of medical products, and more specifically, it relates to a bio-gel repair patch preparation device, a preparation method and an application thereof. Background Art

[0002] In recent years, with the acceleration of population aging, the incidence of geriatric diseases represented by chronic wounds and defective fractures has increased significantly. Autologous platelet plasma therapy has been increasingly used as an adjuvant therapy in various surgeries, which can effectively promote the repair and regeneration of damaged tissues. Currently, this technology is often applied clinically in the form of blood product gels. Blood product gels (taking platelet-rich plasma gel as an example) usually form a fibrin polymerization network into a hydrogel after plasma fibrinogen is catalyzed by thrombin. However, the currently commonly used blood product gels have many defects such as loose structure, fast degradation rate, low mechanical strength, high water content, low content of active factors per unit volume, and inconvenient clamping and use, which cannot meet various requirements such as filling, suturing, and wrapping in clinical surgeries. Summary of the Invention

[0003] In order to overcome the above deficiencies, the present invention provides a bio-gel repair patch preparation device, which not only facilitates the preparation of bio-gel repair patches, but also the prepared bio-gel repair patches have a compact structure and good mechanical strength.

[0004] To solve the above technical problems, the present invention adopts the following technical solutions: A bio-gel repair patch preparation device includes a preparation device body. A detachable tray is installed on the preparation device body. A water filtration membrane is loaded on the tray. Water filtration holes are provided on the tray. A piston that can be pierced by a needle is installed in the preparation device body. A forming cavity is formed between the piston and the water filtration membrane in the preparation device body. When the piston moves, the air pressure in the forming cavity increases, and the water in the gel formed on the water filtration membrane is extruded from the water filtration membrane.

[0005] When the preparation device is in use, a syringe needle is inserted into the piston to the forming cavity, and thrombin and blood are injected into the forming cavity together. After the thrombin and blood are mixed, a layer of gel is formed on the water filtration membrane. Then the piston is moved downward so that the gel formed on the water filtration membrane in the forming cavity is subjected to a unidirectional pressure, and the water in the gel is extruded through the water filtration membrane. During the extrusion process, due to the unidirectional outlet only at the water filtration membrane, the water above the gel will, under pressure, break the wrapping of the lower fibrin network, prompting the gel structure to be reorganized to form an arrangement structure with a certain orientation. This arrangement can facilitate cell migration and adhesion. In addition, the pressure causes the fibrin network to contract, forming a more dense structure, effectively improving the mechanical strength of the patch and facilitating operation.

[0006] The preparer not only facilitates the preparation of the biogel repair patch, but also the prepared biogel repair patch has a compact structure and good mechanical strength.

[0007] Preferably, a number of sunken diversion grooves are provided on the upper surface of the tray.

[0008] The diversion grooves have a diversion function, which is conducive to the rapid discharge of the water extruded by the water filtration membrane.

[0009] Preferably, the preparer body includes an upper cover and a lower cover, and the tray is installed between the upper cover and the lower cover.

[0010] Installing the tray between the upper cover and the lower cover facilitates disassembly and assembly.

[0011] Preferably, a limit groove is provided on the inner wall of the upper opening of the lower cover, and a limit protrusion is provided on the tray, and the limit protrusion is installed in the limit groove.

[0012] The limit protrusion on the tray is installed in the limit groove on the lower cover to circumferentially limit the tray and prevent the tray from rotating.

[0013] Preferably, a convex ring extending upward is provided at the edge of the tray, and a positioning groove is provided on the lower end surface of the upper cover, and the convex ring is fitted and installed in the positioning groove.

[0014] The convex ring on the tray is installed in the positioning groove on the upper cover, making the installation of the tray more tightly and reliably, and ensuring the sealing performance of the connection position.

[0015] Preferably, a number of ventilation grooves are provided on the inner wall of the upper cover, and the ventilation grooves extend to the upper end of the upper cover.

[0016] When the syringe needle injects thrombin and blood into the forming cavity, the piston is placed above the upper cover. At this time, the ventilation groove communicates between the forming cavity and the external environment, making the forming cavity communicate with the external environment, and preventing the pressure in the forming cavity from being too large during the process of injecting thrombin and blood by the syringe needle to cause an obstacle to the injection of thrombin and blood.

[0017] Preferably, a core rod is installed on the upper cover, a needle channel is provided on the core rod, and the piston is connected to the core rod.

[0018] The connection between the piston and the core rod facilitates pushing and pulling movement.

[0019] The present invention also provides a method for preparing a biogel repair patch, which uses a biogel repair patch preparer to prepare a biogel repair patch, and includes the following steps: S1, the piston moves upward to make the forming cavity communicate with the external environment; S2, insert the syringe needle into the piston to the forming cavity. The syringe is provided with a thrombin injection cylinder and a blood injection cylinder. The thrombin injection cylinder is filled with thrombin, and the blood injection cylinder is filled with blood; S3. Push the syringe to mix the thrombin and blood and inject them into the molding cavity, where they are deposited on the water filtration membrane to form a layer of gel. S4. Pull out the needle, and the piston moves downward to make the molding cavity form a closed cavity. The air pressure in the molding cavity increases, and the moisture in the gel is extruded through the water filtration membrane. S5. Remove the tray and take off the gel on the water filtration membrane. The taken-off gel is the prepared biological gel repair patch.

[0020] When preparing the biological gel repair patch, the mixed thrombin and blood form a layer of gel on the water filtration membrane. The piston movement makes the gel subject to a unidirectional pressure, and the moisture in the gel is extruded through the water filtration membrane. Under pressure, the moisture above the gel will damage the wrapping of the underlying fibrin network, promoting the restructuring of the gel structure to form an arrangement structure with a certain orientation. This arrangement facilitates cell migration and adhesion. In addition, the pressure causes the fibrin network to contract, forming a denser structure. The dense fibrin network can better encapsulate blood components such as activated platelets, nucleated cells, and red blood cells, preventing them from entering the recipient's body with the flushing of body fluids, reducing the concentration at the affected area, and reducing the inflammatory stimulation to the affected area. At the same time, it can also prolong the absorption time of the gel, continuously release factors to promote repair, and reduce the number of treatments. The biological gel repair patch prepared by this method is suitable for various application scenarios such as stuffing and supporting, suture fixation, and dressing the wound surface. Moreover, the biological gel repair patch effectively reduces moisture, reduces exudation during wound healing, and reduces the probability of wound infection.

[0021] (1) The biological gel repair patch formed after preparation by the biological gel patch preparation device and the naturally formed fibrin glue have the following advantages: a) Reduce the moisture in the gel, reduce exudation during wound healing, and reduce the probability of wound infection; b) Reduce the gel pore size, enhance the encapsulation ability of cells and active factors, and enhance the sustained release ability; c) Increase the gel porosity, promote the reshaping of the gel structure, show a certain arrangement, and be beneficial to cell adhesion and proliferation.

[0022] (2) The biological gel repair patch formed after being pressed by the biological gel repair patch preparation device can greatly enhance the mechanical strength of the gel compared with natural molding, making the gel suitable for various application scenarios such as stuffing and supporting, suture fixation, and dressing the wound surface.

[0023] (3) Compared with the self-formed biological gel, the unidirectionally extruded and formed biological gel has a super-high concentration of active factors and has a sustained release ability, and can continuously and effectively play a therapeutic role at the affected area, reducing the number of artificial interventions and the risk of infection.

[0024] (4)The bioremediation patch for preparation can be applied to the treatment of acute and chronic wounds, promoting rapid wound repair, collagen generation in the wound, and rapid ingrowth of vascularization, which is significantly superior to the non-extruded prepared gel of the same volume.

[0025] Preferably, after S4, leave it standing for 2 - 4 minutes and then perform S5.

[0026] Leave it standing for a while after extrusion and then remove the gel, which can fully drain the moisture.

[0027] Preferably, in S2, two blood injection syringes are provided. One blood injection syringe contains whole blood, and the other blood injection syringe contains platelet-rich plasma; in S3, first inject the platelet-rich plasma and part of the thrombin into the forming cavity simultaneously and deposit them on the water filtration membrane to form a layer of gel, and then inject the whole blood and the remaining thrombin into the forming cavity simultaneously and deposit them on the water filtration membrane to form the second layer of gel, thereby forming a gel with a double-layer structure.

[0028] Through this method, a gel with a double-layer structure can be formed, that is, a double-layer biogel repair patch is formed, which has a good repair effect on wounds and a long action time.

[0029] The present invention also provides an application of a biogel repair patch. A full-thickness skin is excised from the root of the ear of an adult rabbit to construct a wound model; the biogel repair patch is covered on the wound, and a thin film dressing is covered; the results show that the gel patch prepared by the preparation device can significantly promote wound healing, and the healing speed is significantly greater than the natural healing speed.

[0030] The present invention also provides another application of a biogel repair patch. A full-thickness skin is excised from the back of an adult rabbit to construct a wound model; the biogel repair patch is covered on the wound, and a thin film dressing is covered; the platelet-rich gel and whole blood gel prepared without extrusion of the same volume are used as controls; the gel patch prepared by the preparation device can significantly promote wound healing, the healing speed is significantly greater than the natural healing speed, and it is also superior to the repair speed of the wound in the non-extruded preparation control group.

[0031] The biogel repair patch acts on the affected area through suitable methods such as fitting, filling, suturing, and wrapping, so that the gel patch can be long-term adhered to the affected area to play a therapeutic effect. After the gel patch is adhered to the affected area, the blood components wrapped in it continuously release growth factors and inflammatory factors in the gel, recruiting relevant cells of the receptor to repair the affected area. Among them, growth factors such as PDGF, VEGF, and TGF-β derived from platelets can promote the growth of blood vessels and the generation of collagen in the affected area. Nucleated cells and cross-linked red blood cells will recruit inflammatory cell infiltration, accelerate the clearance of infection in the affected area, and promote the rapid repair of the affected area.

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The preparation device not only facilitates the preparation of the biological gel repair patch, but also the prepared biological gel repair patch has a compact structure and good mechanical strength, and is suitable for various application scenarios such as stuffing and supporting, suture fixation, and dressing the wound surface. Moreover, the biological gel repair patch effectively reduces moisture, reduces exudate during wound healing, and reduces the probability of wound infection; (2) The biological gel after unidirectional extrusion molding has a super-high concentration of active factors and has a slow-release ability, which can continuously and effectively play a therapeutic role at the affected area, reduce the number of times of manual intervention, and reduce the risk of infection; (3) The biological gel repair patch can be applied to the treatment of acute and chronic wounds, promote the rapid repair of the wound surface, promote the generation of collagen in the wound surface, and the rapid ingrowth of vascularization, which is significantly better than the non-extrusion-prepared gel of the same volume. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is a schematic structural diagram of the preparation device of the present invention; Figure 2 is a sectional view of the preparation device of the present invention; Figure 3 is an exploded view of the preparation device of the present invention; Figure 4 is the biological gel repair patch prepared in Example 1 of the present invention; Figure 5 is the biological gel repair patch prepared in Example 2 of the present invention; Figure 6 is the biological gel repair patch prepared in Example 3 of the present invention; Figure 7 is the biological gel repair patch prepared in Example 4 of the present invention; Figure 8 is an analysis diagram of the mechanical strength of the biological gel repair patch of the present invention; Figure 9 is an analysis diagram of the microscopic structure of the biological gel repair patch of the present invention; Figure 10 is an evaluation diagram of the encapsulation capacity of the biological gel repair patch of the present invention; Figure 11 is an evaluation diagram of the growth factor release performance of the biological gel repair patch of the present invention; Figure 12 is an in-vivo degradation diagram of the biological gel repair patch of the present invention; Figure 13 is a diagram of the effect of the biological gel repair patch of the present invention in promoting the repair of the ear skin wound surface; Figure 14 is a diagram of the effect of the biological gel repair patch of the present invention in promoting the repair of the back skin wound surface; In the figure: 1. Preparation device body; 2. Tray; 3. Water filtration membrane; 4. Water filtration holes; 5. Flow guide grooves; 6. Piston; 7. Molding cavity; 8. Upper cover; 9. Lower cover; 10. Flange; 11. Limit groove; 12. Limit protrusion; 13. Support rib; 14. Convex ring; 15. Positioning groove; 16. Ventilation groove; 17. Core rod; 18. Needle channel; 19. Chuck; 20. Card slot; 21. Annular depression; 22. Push-pull handle; 23. Water storage cavity; 24. Ventilation hole. Detailed implementation mode

[0034] The following is a further specific description of the technical solution of the present invention through specific embodiments and in conjunction with the accompanying drawings: Embodiment 1: A preparation device for a biological gel repair patch (see attached Figure 1 to attached Figure 3 ), including a preparation device body 1, a detachable tray 2 is installed on the preparation device body, a water filtration membrane 3 is loaded on the tray, a number of water filtration holes 4 are provided on the tray, a number of sunken flow guide grooves 5 are provided on the upper surface of the tray, a piston 6 that can be pierced by a needle is installed in the preparation device body, a molding cavity 7 is formed between the piston and the water filtration membrane in the preparation device body, and the movement of the piston increases the air pressure in the molding cavity to squeeze the water in the gel formed on the water filtration membrane out of the water filtration membrane.

[0035] The preparation device body includes an upper cover 8 and a lower cover 9, and the tray is installed between the upper cover and the lower cover. The upper cover and the lower cover are connected by threads, a flange 10 is provided on the outer wall of the lower cover, and the flange fits with the lower end of the upper cover. A limit groove 11 is provided on the inner wall of the upper end opening of the lower cover, a limit protrusion 12 is provided on the tray, and the limit protrusion is installed in the limit groove. A number of support ribs 13 are provided on the inner wall of the lower cover, and the tray is supported on the support ribs. An upward-extending convex ring 14 is provided at the edge of the tray, a positioning groove 15 is provided on the lower end surface of the upper cover, and the convex ring is adaptively installed in the positioning groove. The water filtration membrane is clamped between the tray and the lower end of the upper cover.

[0036] A number of ventilation grooves 16 are provided on the inner wall of the upper cover, the upper end of the ventilation groove extends to the upper end of the upper cover, and the lower end of the ventilation groove is located in the middle of the upper cover. A core rod 17 is installed on the upper cover, the lower end of the core rod is placed in the upper cover, a needle channel 18 is provided on the core rod, the needle channel penetrates through the upper and lower ends of the core rod, the aperture of the needle channel gradually decreases from top to bottom, the piston is connected to the core rod, the piston is made of rubber material, a chuck 19 is provided at the lower end of the core rod, an inverted T-shaped card slot 20 adapted to the chuck is provided on the upper part of the piston, and the card slot is clamped and connected to the chuck. The outer wall of the piston is in close fit with the inner wall of the molding cavity, and a number of annular depressions 21 are provided on the outer wall of the piston. A push-pull handle 22 is provided at the upper end of the core rod. A water storage cavity 23 is provided in the lower cover, the water storage cavity is located below the tray, and a ventilation hole 24 communicating with the water outlet cavity is provided at the upper part of the lower cover.

[0037] A method for preparing a biological gel repair patch, using the preparation device for a biological gel repair patch to prepare a biological gel repair patch, including the following steps: S1. Pull the piston upward through the core rod to connect the forming cavity with the external environment through the ventilation groove; S2. Centrifuge the whole blood directly, aspirate 10 mL of the platelet-rich plasma obtained from the upper layer with a blood syringe, load 1 mL of medicinal thrombin into the thrombin syringe, set the thrombin syringe and the blood syringe on the syringe, and insert the syringe needle into the needle track and penetrate the piston into the forming cavity; S3. Push the syringe to mix and inject the thrombin and blood into the forming cavity, and deposit them on the water filtration membrane to form a layer of gel; S4. Pull out the needle, press the piston downward through the core rod. The piston moves downward to make the forming cavity form a closed cavity. The air pressure in the forming cavity increases to extrude the water in the gel through the water filtration membrane. Let it stand for 2 - 4 minutes, preferably 3 minutes; S5. Unscrew the upper cover and the lower cover, remove the tray, take off the gel on the water filtration membrane. The taken gel is the prepared biological gel repair patch (see attachment Figure 4 ).

[0038] Example 2: A method for preparing a biological gel repair patch, which uses a biological gel repair patch preparation device to prepare the biological gel repair patch. The steps are similar to those in Example 1. The main difference is that in S2 of this example, a commercially available platelet-rich plasma preparation set is used to prepare the platelet-rich plasma, and the other steps are the same as those in Example 1. The prepared biological gel repair patch is as shown in the attachment Figure 5 as shown.

[0039] Example 3: A method for preparing a biological gel repair patch, which uses a biological gel repair patch preparation device to prepare the biological gel repair patch. The steps are similar to those in Example 1. The main difference is that in S2 of this example, two blood syringes are set. One blood syringe contains whole blood, and the other blood syringe contains platelet-rich plasma; in S3, first inject the platelet-rich plasma and part of the thrombin into the forming cavity at the same time, and deposit them on the water filtration membrane to form a layer of gel, then inject the whole blood and the remaining thrombin into the forming cavity at the same time, and deposit them on the water filtration membrane to form a second layer of gel, so as to form a gel with a double-layer structure. The other steps are the same as those in Example 1. The prepared biological gel repair patch is as shown in the attachment Figure 6 as shown.

[0040] Example 4: A method for preparing a bio-gel repair patch, which uses a bio-gel repair patch preparation device to prepare the bio-gel repair patch. The steps are similar to those in Example 1. The main difference is that in this example, whole blood is used to prepare the bio-gel repair patch in S2. The blood syringe aspirates 10 mL of whole blood, and 1 mL of medicinal thrombin is loaded into the thrombin syringe. The thrombin syringe and the blood syringe are set on the syringe. The syringe needle is inserted into the needle channel and penetrates through the piston into the molding cavity; other steps are the same as those in Example 1. The prepared bio-gel repair patch is as shown in Figure 7 the following figure.

[0041] Mechanical strength analysis of the bio-gel repair patch in Examples 1-4: The maximum tensile force that the bio-gel repair patches prepared in Examples 1-4 can withstand is tested by a universal tensile machine. The results are as shown in Figure 8 the following figure. The maximum tensile force that the platelet gel patch can withstand after being pressed and formed by the preparation device is much greater than the tensile force before pressing. The pressure of the double-layer patch also increases, but the pressure of the whole blood patch remains basically unchanged.

[0042] Microstructure analysis of the bio-gel repair patch in Examples 1-4: The microstructure of the bio-gel repair patches prepared in Examples 1-4 is observed by a cryo-scanning electron microscope. The results are as shown in Figure 9 the following figure. The pore size of the prepared bio-gel repair patch is significantly reduced, the fibrin network becomes denser, and a longitudinally arranged structure appears. The structural difference at the junction of the double-layer bio-gel repair patch in Example 3 is obvious. The platelet gel shows longitudinal arrangement, and the fibrin network of the whole blood layer is dense, providing different mechanical properties.

[0043] Evaluation of the encapsulation ability of the bio-gel repair patch in Examples 1-4: The blood cell content in the liquid discharged during the preparation process of Examples 1-4 is detected. The results are as shown in Figure 10 the following figure. The bio-gel repair patches in Examples 1-3 have good encapsulation performance for various blood cells in the gel. The blood cell encapsulation ability of Example 4 decreases compared with Examples 1-3.

[0044] Evaluation of the growth factor release performance of the bio-gel repair patch: The bio-gel repair patch prepared in Example 1 is extracted with serum-free medium for 72 hours, and the same volume of unprepared platelet-rich gel is used as a control. The results are as shown in Figure 11 the following figure. Within 72 hours, the growth factors PDGF-BB, bFGF, VEGF, TGF-β, and PF4 released by the extruded bio-gel repair patch are significantly greater than those of the unprepared platelet-rich gel. It is suggested that the gel patch has a higher growth factor loading and release ability after being pressed by the preparation device.

[0045] In vivo degradation of the biogel repair patch: The biogel repair patch prepared in Example 1 was implanted subcutaneously in adult New Zealand rabbits, and platelet-rich gel prepared without extrusion of the same volume was used as a control. The results are as Figure 12 shown. The biogel repair patch was not degraded on the 15th day after implantation, while the control was completely degraded on the 7th day. It is suggested that the gel patch delays the degradation rate after being pressed by the preparation device.

[0046] Effect of the biogel repair patch on promoting the repair of ear skin wounds: A full-thickness skin with a diameter of 1.5 cm was excised from the root of the ear of adult New Zealand rabbits to construct a wound model. The biogel repair patch of Example 1 was covered on the wound, and a thin film dressing was used to cover it. The results showed that the gel patch prepared by the preparation device could significantly promote wound healing, and the healing rate was significantly higher than the natural healing rate (see Appendix Figure 13 ). By analyzing and comparing the HE staining of skin sections, the number of newly formed blood vessels and the number of newly formed hair follicles in the biogel repair patch repair group on the 12th day were significantly better than those in the blank group with natural growth repair. Masson staining showed that the biogel repair patch could promote wound collagen production more effectively (see Appendix Figure 13 ). Combining the above experimental results, it is suggested that the gel patch after being pressed by the preparation device can more effectively promote the healing of ear skin wounds.

[0047] Effect of the biogel repair patch on promoting the repair of back skin wounds: A full-thickness skin with a diameter of 2 cm was excised from the back of adult New Zealand rabbits to construct a wound model. The biogel repair patches prepared in Example 1 and Example 4 were covered on the wound, and a thin film dressing was used to cover it. Platelet-rich gel and whole blood gel prepared without extrusion of the same volume were used as controls. As Figure 14 shown, the gel patch prepared by the preparation device could significantly promote wound healing, and the healing rate was significantly higher than the natural healing rate and also better than the repair rate of the non-extruded control group. By analyzing and comparing the HE staining of skin sections of each group, the number of newly formed blood vessels and the number of newly formed hair follicles in the biogel repair patch repair group were significantly better than those in the blank group with natural growth repair and the non-extruded gel group. Combining the above experimental results, it is suggested that the gel patch after being pressed by the patch preparation device can more effectively promote wound healing.

[0048] The biogel repair patches obtained in Examples 2-4 were respectively tested for growth factor release performance, in vivo degradation, effect on promoting ear skin wound repair, and effect on promoting back skin wound repair. The results were similar to those of the platelet-rich gel patch in Example 1, indicating that similar biogel repair patches can be prepared by adjusting the addition ratios of the above-mentioned plasma and whole blood.

[0049] The above-described embodiments are only preferred solutions of the present invention, and do not impose any form of limitation on the present invention. There are other variations and modifications without exceeding the technical solutions described in the claims.

Claims

1. A biological gel repair patch preparation device, characterized in that It includes a preparation device body, a detachable tray is installed on the preparation device body, a water filtration membrane is loaded on the tray, water filtration holes are provided on the tray, a piston that can be pierced by a needle is installed in the preparation device body, a forming cavity is formed between the piston and the water filtration membrane in the preparation device body, and the movement of the piston increases the air pressure in the forming cavity to squeeze the water in the gel formed on the water filtration membrane out of the water filtration membrane.

2. The preparation device of the biological gel repair patch according to claim 1, characterized in that, A number of sunken diversion grooves are provided on the upper surface of the tray.

3. The bio-gel repair patch preparation device according to claim 1, characterized in that, The preparation device body includes an upper cover and a lower cover, and the tray is installed between the upper cover and the lower cover.

4. The biogel repair patch preparation device according to claim 3, characterized in that, A limiting groove is provided on the inner wall of the upper end opening of the lower cover, a limiting protrusion is provided on the tray, and the limiting protrusion is installed in the limiting groove.

5. A biogel repair patch preparation device according to claim 3, characterized in that, A convex ring extending upward is provided at the edge of the tray, a positioning groove is provided on the lower end surface of the upper cover, and the convex ring is fitted and installed in the positioning groove.

6. The biogel repair patch preparation device according to claim 3, characterized in that A number of ventilation grooves are provided on the inner wall of the upper cover, and the ventilation grooves extend to the upper end of the upper cover.

7. A bio-gel repair patch preparation device according to any one of claims 1 to 6, characterized in that, A core rod is installed on the upper cover, a needle channel is provided on the core rod, and the piston is connected to the core rod.

8. A preparation method of a biological gel repair patch, characterized in that Using the biogel repair patch preparation device according to any one of claims 1 to 7 to prepare a biogel repair patch, including the following steps: S1, the piston moves upward to connect the forming cavity with the external environment; S2, insert the syringe needle into the piston to the forming cavity, the syringe is provided with a thrombin injection cylinder and a blood injection cylinder, the thrombin injection cylinder is filled with thrombin, and the blood injection cylinder is filled with blood; S3, push the syringe to mix and inject the thrombin and blood into the forming cavity, and deposit on the water filtration membrane to form a layer of gel; S4, pull out the needle, the piston moves downward to make the forming cavity form a closed cavity, and the air pressure in the forming cavity increases to squeeze the water in the gel out through the water filtration membrane; S5, remove the tray, take off the gel on the water filtration membrane, and the taken-off gel is the completed biogel repair patch.

9. The preparation method of the biogel repair patch according to claim 8, characterized in that, After S4, wait for 2 - 4 minutes and then perform S5.

10. According to the biogel repair patch preparation method described in claim 8, it is characterized in that in S2, two blood injection cylinders are provided, one blood injection cylinder is filled with whole blood, and the other blood injection cylinder is filled with platelet-rich plasma; in S3, first inject the platelet-rich plasma and part of the thrombin into the forming cavity at the same time, and deposit on the water filtration membrane to form a layer of gel, then inject the whole blood and the remaining thrombin into the forming cavity at the same time, and deposit on the water filtration membrane to form a second layer of gel, so as to form a gel with a double-layer structure.

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