一种基于CPA-双CISPR / Cas12b检测PVC2病毒的组合物及其检测方法与应用
By optimizing the CPA-dual CISPR/Cas12b system and a specific crRNA concentration ratio, combined with time-resolved immunofluorescence microspheres and lateral flow chromatography strips, the problems of low sensitivity, complex operation, and strong equipment dependence in existing PCV2 virus detection have been solved, achieving rapid, simple, and accurate virus detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGDONG MEDICAL UNIV
- Filing Date
- 2025-05-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing PCV2 virus detection methods suffer from low sensitivity, complex operation, strong equipment dependence, high false positive rate, high cost, and difficulty in meeting the needs of rapid diagnosis, making them unsuitable for widespread application in resource-limited grassroots farms or in the field.
By employing the CPA-dual CISPR/Cas12b system, combining crRNA-2 and crRNA-4 at specific concentration ratios, optimizing the CPA reaction reagents and CRISPR/Cas12b system, and using time-resolved immunofluorescent microspheres and optimized lateral flow chromatography test strips, rapid and convenient PCV2 virus detection can be achieved.
It improves detection sensitivity and signal-to-noise ratio, simplifies operation procedures, reduces equipment dependence, is suitable for field use, reduces costs, and achieves rapid and accurate PCV2 virus detection.
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Figure CN120310964B_ABST