Method and device for determining fishy smell substances, test equipment and storage medium

Abstract

The invention relates to the technical field of detection, and discloses a method for measuring fishy smell substances, which comprises the following steps: mixing and stirring a sample and an internal standard substance 2, 4, 6-trimethylpyridine to obtain a mixed sample; carrying out headspace extraction on the mixed sample to generate a to-be-determined substance containing the fishy smell substance; and measuring the fishy smell substance in the substance to be measured by using a gas chromatograph-mass spectrometer. 2, 4, 6-trimethylpyridine is used as an internal standard substance to be mixed with a sample, the chemical property of 2, 4, 6-trimethylpyridine is uniform and stable, and the 2, 4, 6-trimethylpyridine can be better used as a benchmark for characteristic substances in fishy smell substances of fishes. After the mixed sample is subjected to headspace extraction, the gas chromatograph-mass spectrometer is used for determining the fishy smell substances in the to-be-determined substances, so that the test result is close to the real situation, and the accuracy of the test result is improved. The invention further discloses a device for measuring the fishy smell substance, testing equipment and a storage medium.

Description

Technical Field

[0001] This application relates to the field of detection technology, for example, to a method and device for determining fishy smell substances, a testing device, and a storage medium. Background Art

[0002] Currently, aquatic products are one of the main sources of high-quality animal protein in human food. However, the peculiar smell of some farmed aquatic products makes it difficult for consumers to accept, which has been a problem plaguing the aquaculture industry for a long time and directly affects the consumption of aquatic products and the economic benefits of aquaculture. Taking fish as an example, when the concentration of fishy smell substances in fish meat exceeds a certain standard, it will affect the eating experience of consumers. Therefore, it is necessary to detect the content of fishy smell substances in fish meat.

[0003] Combined with Figure 1 As shown, the related technology provides a method for determining fishy smell substances in surplus water, including: sucking the accumulated water at the bottom of the box as a sample, performing chromatographic column separation through an injector under the action of a carrier gas, and finally quantitatively analyzing the sample components in a detector to measure and record the total content of fishy smell substances in the surplus water.

[0004] In the process of implementing the embodiments of the present disclosure, it is found that at least the following problems exist in the related technology:

[0005] Although the related technology can detect the amount of fishy smell substances in fish meat, the data fluctuates greatly among multiple batches of samples, is unstable, and has low accuracy.

[0006] It should be noted that the information disclosed in the above background art section is only used to enhance the understanding of the background of this application, and therefore may include information that does not constitute the prior art known to those of ordinary skill in the art. Summary of the Invention

[0007] To have a basic understanding of some aspects of the disclosed embodiments, a simple summary is given below. This summary is not a general review, nor is it intended to identify key / important constituent elements or delineate the protection scope of these embodiments, but rather serves as a preface to the subsequent detailed description.

[0008] The embodiments of the present disclosure provide a method and device for determining fishy smell substances, a testing device, and a storage medium to improve the accuracy of detecting the content of fishy smell substances.

[0009] In some embodiments, the method for determining fishy smell substances includes: mixing a sample and an internal standard, 2,4,6-trimethylpyridine, and stirring to obtain a mixed sample; performing headspace extraction on the mixed sample to generate a substance to be determined containing fishy smell substances; and using a gas chromatography-mass spectrometry instrument to determine the fishy smell substances in the substance to be determined.

[0010] Optionally, mixing the sample with the internal standard 2,4,6-trimethylpyridine includes: mixing the sample and the 2,4,6-trimethylpyridine solution in a ratio of 3 - 5 mL∶10 μL of the sample to the 2,4,6-trimethylpyridine solution; wherein, the concentration of the 2,4,6-trimethylpyridine solution is 20.00 - 22.00 ppm.

[0011] Optionally, stirring includes: stirring at a temperature of 55 - 60 °C for 8 - 10 min.

[0012] Optionally, performing headspace extraction on the mixed sample includes: performing headspace extraction at a temperature of 55 - 60 °C for 30 - 40 min.

[0013] Optionally, using a gas chromatography - mass spectrometry (GC - MS) instrument to determine the fishy odor substances in the substance to be determined includes: separating each component in the substance to be determined using a gas chromatograph; detecting each component separately using a mass spectrometer; processing the detection results to obtain the content of the fishy odor substances in the substance to be determined.

[0014] Optionally, separating each component in the substance to be determined using a gas chromatograph includes: under a temperature programming, using the gas chromatograph to extract each component in the substance to be determined at a flow rate of 0.5 - 1.5 mL / min; wherein, the chromatographic column of the gas chromatograph is a 30 m × 0.25 mm, 0.25 μm elastic capillary column; the carrier gas of the gas chromatograph is helium.

[0015] Optionally, the temperature programming includes: the first stage, the chromatographic column is kept at an initial temperature of 40 - 50 °C for 2 - 3 min; the second stage, the temperature of the chromatographic column is increased from the initial temperature to 230 - 240 °C at a heating rate of 8 - 10 °C / min; the third stage, the temperature of the chromatographic column is kept at 230 - 240 °C for 5 - 6 min.

[0016] Optionally, detecting each component separately using a mass spectrometer includes: setting the mass spectrometer according to the following parameters: the temperatures of the injection port and the interface are the same, both being 240 - 250 °C; using an electron impact ionization source, the electron energy is 60 - 70 eV, the emission current is 1 - 1.5 mA, the temperature is 200 - 210 °C; the detector voltage is 1700 - 1800 V; the scanning range is 33 - 400 amu.

[0017] Optionally, processing the detection results to obtain the content of the fishy odor substances in the substance to be determined includes: automatically retrieving the detection results in a database; selecting the detection results with a purity and a matching degree both greater than 800; performing normalization processing and analysis on the selected detection results to obtain the content of the fishy odor substances in the substance to be determined.

[0018] In some embodiments, the device for measuring fishy smell substances includes a processor and a memory storing program instructions. The processor is configured to execute the method for measuring fishy smell substances as described above when running the program instructions.

[0019] In some embodiments, the test device includes a gas chromatography-mass spectrometry (GC-MS) instrument and also includes the device for measuring fishy smell substances as described above, which is installed in the test device.

[0020] In some embodiments, the storage medium includes a computer-readable storage medium storing program instructions. When the program instructions are running, they are used to cause a computer to execute the method for measuring fishy smell substances as described above.

[0021] The method, device, test equipment, and storage medium for measuring fishy smell substances provided by the embodiments of the present disclosure can achieve the following technical effects:

[0022] In the embodiments of the present disclosure, 2,4,6-trimethylpyridine is used as an internal standard and mixed with the sample. 2,4,6-trimethylpyridine has uniform and stable chemical properties, and for the characteristic substances in fishy smell substances, 2,4,6-trimethylpyridine can be used as a better reference. After the mixed sample is subjected to headspace extraction, a gas chromatography-mass spectrometry (GC-MS) instrument is used to measure the fishy smell substances in the substance to be measured, making the test results closer to the real situation, thereby improving the accuracy of the test results.

[0023] The above general description and the following description are only exemplary and explanatory, and are not used to limit this application. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] One or more embodiments are exemplarily illustrated by corresponding drawings. These exemplary illustrations and the drawings do not constitute a limitation on the embodiments. Elements with the same reference numerals in the drawings are shown as similar elements. The drawings do not constitute a scale limitation, and:

[0025] Figure 1 is a schematic diagram of a method for measuring fishy smell substances in surplus water provided by the related art;

[0026] Figure 2 is a schematic diagram of a method for measuring fishy smell substances provided by the embodiments of the present disclosure;

[0027] Figure 3 is a schematic diagram of the system structure of a gas chromatography-mass spectrometry (GC-MS) instrument;

[0028] Figure 4 is an application schematic diagram of an embodiment of the present disclosure;

[0029] Figure 5It is a schematic diagram of a device for measuring fishy substances provided by an embodiment of the present disclosure;

[0030] Figure 6 It is a schematic diagram of a test device provided by an embodiment of the present disclosure. Specific embodiments

[0031] In order to be able to understand the features and technical content of the embodiments of the present disclosure in more detail, the implementation of the embodiments of the present disclosure will be described in detail below with reference to the accompanying drawings. The accompanying drawings are only for reference and explanation, and are not used to limit the embodiments of the present disclosure. In the following technical description, for the sake of explanation, numerous details are provided to give a full understanding of the disclosed embodiments. However, one or more embodiments may still be implemented without these details. In other cases, well-known structures and devices may be shown in a simplified manner to simplify the drawings.

[0032] In the specification and claims of the embodiments of the present disclosure and the above-mentioned drawings, terms such as "first" and "second" are used to distinguish similar objects, and do not necessarily have to be used to describe a specific order or sequence. It should be understood that such data may be interchanged under appropriate circumstances so as to implement the embodiments of the present disclosure described herein. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion.

[0033] Unless otherwise specified, the term "plurality" means two or more.

[0034] In the embodiments of the present disclosure, the character " / " indicates that the objects before and after are in an "or" relationship. For example, A / B means: A or B.

[0035] The term "and / or" is a description of the association relationship of an object, indicating that there can be three relationships. For example, A and / or B means: A or B, or, the three relationships of A and B.

[0036] The term "corresponding" may refer to an association relationship or a binding relationship. A corresponding to B means that there is an association relationship or a binding relationship between A and B.

[0037] When steaming fish in a steam box, due to the vaporization and liquefaction of gas, a large amount of residual water will remain in the inner liner of the steam box. The fishy substances in the fish are easily soluble in water. Therefore, the residual water in the inner liner of the steam box can be used as a sample, and the content of the fishy substances in the residual water can be measured by a testing device to characterize the content of the fishy substances in the original fish. In the residual water, the fishy substances mainly include: hexanal, octanal, nonanal, (E,E)-2,6-nonadienal, 2,4-decadienal, 2,3-octanedione, and 1-octen-3-ol. Among them, the peak area content of octanal and nonanal in the mass spectrometry analysis results is relatively high, and the data such as the peak emergence time are relatively stable and representative. Therefore, in the embodiments of the present disclosure, octanal and nonanal are used as characteristic substances of the fishy substances, and the content of the fishy substances in the residual water is characterized by measuring the content of octanal and nonanal in the residual water. In addition, in order to simulate the normal fish steaming process and improve the accuracy of the measurement result of the fishy substances, during the process of obtaining the sample, the fish also needs to be pretreated. After the fish is washed, its internal organs and fish bones are removed, and the fish meat is stirred into a paste. A certain weight of the fish meat is weighed, spread flat in a steaming tray, and then placed in the steam box to start steaming. After steaming for a preset duration, the residual water in the inner liner of the steam box is collected as a sample.

[0038] Combined Figure 2 As shown, the embodiments of the present disclosure provide a method for measuring fishy substances, including:

[0039] S001, the testing device mixes the sample and the internal standard 2,4,6-trimethylpyridine, and stirs to obtain a mixed sample.

[0040] S002, the testing device performs headspace extraction on the mixed sample to generate a substance to be measured containing fishy substances.

[0041] S003, the testing device uses a gas chromatography-mass spectrometry (GC-MS) instrument to measure the fishy substances in the substance to be measured.

[0042] In the embodiments of the present disclosure, when using a gas chromatography-mass spectrometry (GC-MS) instrument to measure the fishy substances in the substance to be measured, the sample and the internal standard need to be mixed before performing chromatographic analysis and mass spectrometry analysis to improve the accuracy and reliability of the analysis. During the implementation of the embodiments of the present disclosure, the measurement of the fishy substances in the substance to be measured is mainly the measurement of the content of octanal and nonanal in the substance to be measured. Therefore, 2,4,6-trimethylpyridine with uniform and stable chemical properties is selected as the internal standard to be mixed with the sample, which can further improve the accuracy of the measurement result. Then, headspace extraction is performed on the mixed sample to separate each component in the mixed sample from the original mixed sample and generate a substance to be measured containing fishy substances. Finally, chromatographic analysis and mass spectrometry analysis are performed on the substance to be measured, and the content of octanal and nonanal in the substance to be measured, that is, the content of the fishy substances, is obtained according to the analysis results.

[0043] Optionally, mixing the sample with the internal standard 2, 4, 6-trimethylpyridine includes: mixing the sample and the 2, 4, 6-trimethylpyridine solution in a ratio of 3 - 5 mL∶10 μL of the sample to the 2, 4, 6-trimethylpyridine solution; wherein, the concentration of the 2, 4, 6-trimethylpyridine solution is 20.00 - 22.00 ppm.

[0044] In this embodiment, the remaining water in the inner tank of the steaming box is used as the sample. As shown in Table 1, the total amount of remaining water generated by different steaming boxes during each steaming process is different, and multiple parallel samples are required for sampling the sample during the measurement for comparison. Therefore, taking three parallel samples as an example, 3 - 5 mL of the remaining water can be used as a parallel sample for one measurement. After mixing 3 - 5 mL of the sample with 10 μL of the 2, 4, 6-trimethylpyridine solution with a concentration of 20.00 - 22.00 ppm, the content of 2, 4, 6-trimethylpyridine in the mixed sample is equivalent to the content of octanal and nonanal, which can better perform the benchmarking and make the test results more accurate.

[0045] Table 1

[0046]

[0047] Optionally, stirring includes: stirring at a temperature of 55 - 60 °C for 8 - 10 min.

[0048] In the embodiment of the present disclosure, the mixed sample is stirred using a heating magnetic stirrer at a temperature of 55 - 60 °C. The heating magnetic stirrer adopts the principle of magnetic drive and utilizes the eddy current formed on the metal surface by the magnetic force lines in the magnetic field cutting the metal guide bar or guide belt, which can quickly mix the sample and the internal standard. Taking a 3 mL sample as an example, after mixing the sample with the 2, 4, 6-trimethylpyridine solution, it is stirred at a temperature of 55 - 60 °C for 8 min using a heating magnetic stirrer. In the case where the sampling volume of the sample is greater than 3 mL, the stirring time can be appropriately extended to 10 min to fully mix the sample and the internal standard. In addition, when increasing the sampling volume, the deviation of the measurement result will also increase. Therefore, the sampling volume of the sample generally does not exceed 5 mL.

[0049] Optionally, performing headspace extraction on the mixed sample includes: performing headspace extraction at a temperature of 55 - 60 °C for 30 - 40 min.

[0050] Headspace extraction can separate and enrich the compounds in a mixed sample. Other compounds such as 2,4,6-trimethylpyridine, octanal, and nonanal in the mixed sample are injected into a solid-phase adsorption column or a liquid carrier through a sampling needle. For a 3 mL sample mixed solution, headspace extraction is carried out for 30 min to make the mixed solution volatilize to saturation. In the case where the sampling volume of the sample is greater than 3 mL, the headspace extraction time can be appropriately extended to 40 min.

[0051] Optionally, the model of the sampling needle is 57329-U Supelco 50 / 30μm DVB / CAR / PDMS automatic solid-phase microextraction head.

[0052] In the sampling needle selected in this embodiment, 30μm of DVB (divinylbenzene) and CAR (carboxyl) are coated on 50μm of PDMS (polydimethylsiloxane). Using this model of sampling needle, more efficient extraction of the substances to be measured in the mixed sample can be carried out.

[0053] Optionally, a gas chromatography-mass spectrometry (GC-MS) instrument is used to measure the fishy-smelling substances in the substances to be measured, including: separating the various components in the substances to be measured using a gas chromatograph; detecting each component separately using a mass spectrometer; processing the detection results to obtain the content of the fishy-smelling substances in the substances to be measured.

[0054] The substances to be measured are introduced into the gas chromatography-mass spectrometry instrument. First, the various components in the substances to be measured are separated using a gas chromatograph, and then the various components flowing out of the gas chromatograph are sent into the mass spectrometer for detection. The mass spectrometer analyzes each component introduced at the interface to obtain an analysis result. Finally, retrieval processing is carried out according to the analysis result to obtain the content of octanal and nonanal in the substances to be measured.

[0055] Combined Figure 3 As shown, in the gas chromatography-mass spectrometry instrument 400, it includes a chromatographic system 401, a mass spectrometry system 402, and a data system 403. The chromatographic system 401 is used to vaporize the liquid in the vaporization chamber at a high temperature and separate the vaporized compounds through a carrier gas system. The mass spectrometry system 402 obtains the gaseous compounds flowing out of the chromatographic system through an interface device, separates them through a separation system, and then sends them into a detection system for detection. The data system 403 is used to collect data on the detection results and perform retrieval analysis to achieve a qualitative and quantitative analysis effect.

[0056] Optionally, separating the various components in the substances to be measured using a gas chromatograph includes: under a temperature programming, using the gas chromatograph to extract the various components in the substances to be measured at a flow rate of 0.5 - 1.5 mL / min; wherein, the chromatographic column of the gas chromatograph is a 30m × 0.25mm, 0.25μm elastic capillary column; and the carrier gas of the gas chromatograph is helium.

[0057] In the embodiments of the present disclosure, the gas chromatograph extracts each component in the substance to be measured at a flow rate of 0.5-1.5 mL / min, which can improve the extraction efficiency of the substance to be measured. The chromatographic column of the gas chromatograph is selected as a DB-WAX elastic capillary column, with a column length of 30 m, an inner diameter of 0.25 mm, and a fixed-phase coating liquid film thickness of 0.25 μm. The carrier gas of the gas chromatograph is an inert gas, including high-purity helium, etc.

[0058] Optionally, the temperature programming includes: the first stage, the chromatographic column is kept at an initial temperature of 40-50 °C for 2-3 min; the second stage, the temperature of the chromatographic column is raised from the initial temperature to 230-240 °C at a heating rate of 8-10 °C / min; the third stage, the temperature of the chromatographic column is kept at 230-240 °C for 5-6 min.

[0059] The gas chromatograph needs to vaporize the liquid in a high-temperature environment, so the temperature of the chromatographic column needs to be gradually increased to reduce the influence on the chemical properties of each component in the substance to be measured.

[0060] Optionally, the mass spectrometer is used to detect each component separately, including: setting the mass spectrometer according to the following parameters: the temperatures of the injection port and the interface are the same, both are 240-250 °C; an electron impact ionization source is used, the electron energy is 60-70 eV, the emission current is 1-1.5 mA, and the temperature is 200-210 °C; the detector voltage is 1700-1800 V; the scanning range is 33-400 amu.

[0061] Optionally, the detection results are processed to obtain the content of the fishy smell substances in the substance to be measured, including: automatically retrieving the detection results in the database; selecting the detection results with a purity and a matching degree both greater than 800 in the retrieval results; performing normalization processing and analysis on the selected detection results to obtain the content of the fishy smell substances in the substance to be measured.

[0062] After obtaining the test results, Xcalibur software is selected to perform data analysis on the test results, and the fishy substances in the substances to be measured are automatically retrieved from two databases, NIST2017 and willey9. The test results with both purity and matching degree greater than 800 are selected, and then the test results are analyzed using the normalization method, which can further improve the accuracy of the test results. Among them, Xcalibur software is a control and data processing software based on the Windows platform, supporting multiple query methods, and can perform data retrieval and screening according to different attributes, and conduct data search and analysis. The NIST2017 chemical database contains a large amount of standard infrared spectrum data of chemical substances, and the Willey9 academic literature database contains a large number of academic literatures in the fields of chemistry, biomedicine, materials science, engineering, etc. Retrieving and analyzing in these two databases can more accurately determine the fishy substances.

[0063] By using the method provided in the embodiment of the present disclosure to detect the remaining water in the inner tank of the steaming oven, the content of the fishy substances in the remaining water can be more accurately determined. The following takes the test results measured in the process of implementing the embodiment of the present disclosure as an example for illustration.

[0064] Perform the steaming process on the steaming oven 1 three times, collect the remaining water each time, and obtain three different portions of remaining water. Take three samples from each portion of the remaining water, 3 mL each time, and a total of nine samples can be obtained. The three samples obtained from the same portion of the remaining water are mutually parallel control groups. Perform a determination on each sample respectively. As shown in Figure 4 , mix 3 mL of the sample with 10 μL of 2,4,6-trimethylpyridine solution with a concentration of 21.65 ppm, and then after stirring, headspace extraction, chromatographic analysis, mass spectrometry analysis and data processing, calculate the contents of octanal and nonanal respectively, and take the sum of the contents of octanal and nonanal as the content of the fishy substances to obtain the test results of the fishy substances, as shown in Table 2.

[0065] Table 2

[0066]

[0067]

[0068] Repeat the above determination process for the steaming oven 2 and the steaming oven 3, and the test results of the fishy substances in the remaining water of the steaming oven 2 and the steaming oven 3 can be obtained, as shown in Table 3.

[0069] Table 3

[0070]

[0071] Using the method for measuring fishy smell substances provided by the embodiments of the present disclosure, nine different samples in the remaining water of the same steaming box are respectively measured, and three different measurement results are obtained. The obtained measurement result data has small fluctuations and high repeatability, and the measurement results are more accurate.

[0072] Combined with Figure 5 As shown, the embodiments of the present disclosure provide a device 300 for measuring fishy smell substances, including a processor 100 and a memory 101. Optionally, the device may further include a communication interface 102 and a bus 103. Among them, the processor 100, the communication interface 102, and the memory 101 can complete mutual communication through the bus 103. The communication interface 102 can be used for information transmission. The processor 100 can call the logical instructions in the memory 101 to execute the method for measuring fishy smell substances in the above embodiments.

[0073] In addition, when the logical instructions in the above-mentioned memory 101 are implemented in the form of software function units and sold or used as an independent product, they can be stored in a computer-readable storage medium.

[0074] The memory 101, as a computer-readable storage medium, can be used to store software programs and computer-executable programs, such as the program instructions / modules corresponding to the methods in the embodiments of the present disclosure. The processor 100 executes functional applications and data processing by running the program instructions / modules stored in the memory 101, that is, implements the method for measuring fishy smell substances in the above embodiments.

[0075] The memory 101 may include a program storage area and a data storage area. Among them, the program storage area can store an operating system and application programs required for at least one function; the data storage area can store data created according to the use of the terminal device, etc. In addition, the memory 101 may include a high-speed random access memory and may also include a non-volatile memory.

[0076] Combined with Figure 6 As shown, the embodiments of the present disclosure provide a test device 200, including: a gas chromatography-mass spectrometry instrument 400 and the above-mentioned device 300 for measuring fishy smell substances. The device 300 for measuring fishy smell substances is installed in the test device 200. The installation relationship described here not only includes being placed inside the test device 200, but also includes installation connections with other components of the test device 200, including but not limited to physical connections, electrical connections, or signal transmission connections, etc. Those skilled in the art can understand that the device 300 for measuring fishy smell substances can be adapted to a feasible test device 200, and further implement other feasible embodiments.

[0077] An embodiment of the present disclosure provides a storage medium, including a computer-readable storage medium storing computer-executable instructions, and the computer-executable instructions are configured to execute the above method for measuring fishy smell substances.

[0078] The technical solution of the embodiment of the present disclosure can be embodied in the form of a software product. The computer software product is stored in a storage medium and includes one or more instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the method described in the embodiment of the present disclosure. The foregoing storage medium may be a non-transitory storage medium, including: a USB flash drive, a mobile hard disk, a read-only memory (ROM, Read-Only Memory), a random access memory (RAM, Random Access Memory), a magnetic disk, or an optical disc, etc., which are various media that can store program codes.

[0079] The above description and drawings fully illustrate the embodiments of the present disclosure so that those skilled in the art can practice them. Other embodiments may include structural, logical, electrical, process, and other changes. Embodiments merely represent possible variations. Unless explicitly required, separate components and functions are optional, and the order of operations may vary. Parts and features of some embodiments may be included in or substituted for parts and features of other embodiments. Moreover, the terms used in this application are only for describing embodiments and are not used to limit the claims. As used in the description of the embodiments and the claims, unless the context clearly indicates otherwise, the singular forms "a", "an", and "the" are intended to also include the plural forms. Similarly, as used in this application, the term "and / or" refers to any and all possible combinations including one or more of the associated listed items. Additionally, when used in this application, the term "comprise" and its variants "comprises" and / or "comprising" etc. mean the presence of the stated features, wholes, steps, operations, elements, and / or components, but do not exclude the presence or addition of one or more other features, wholes, steps, operations, elements, components, and / or groupings thereof. Without further limitation, an element defined by the statement "comprising an..." does not exclude the presence of additional identical elements in the process, method, or device including the element. In this document, what each embodiment focuses on may be the differences from other embodiments, and the same or similar parts among the various embodiments may be referred to each other. For the methods, products, etc. disclosed in the embodiments, if they correspond to the method part disclosed in the embodiments, the relevant parts may refer to the description of the method part.

[0080] Those skilled in the art can realize that the units and algorithm steps of each example described in combination with the embodiments disclosed herein can be implemented by electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are executed in a hardware or software manner may depend on the specific application and design constraints of the technical solution. The skilled person can use different methods for each specific application to implement the described functions, but such implementation should not be considered to exceed the scope of the embodiments of the present disclosure. The skilled person can clearly understand that for the convenience and conciseness of description, the specific working processes of the systems, devices, and units described above can refer to the corresponding processes in the foregoing method embodiments, and will not be elaborated herein.

[0081] In the embodiments disclosed herein, the disclosed methods, products (including but not limited to devices, equipment, etc.) can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the division of the units can be merely a logical function division, and there can be other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the couplings, direct couplings, or communication connections shown or discussed with each other can be through some interfaces, and the indirect couplings or communication connections of the devices or units can be in electrical, mechanical, or other forms. The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they can be located in one place or distributed to multiple network units. Some or all of the units can be selected according to actual needs to implement this embodiment. In addition, the functional units in the embodiments of the present disclosure can be integrated into one processing unit, or each unit can exist physically alone, or two or more units can be integrated into one unit.

[0082] The flowcharts and block diagrams in the accompanying drawings illustrate the architecture, functionality, and operation of possible implementations of systems, methods, and computer program products according to embodiments of the present disclosure. In this regard, each block in the flowchart or block diagram may represent a module, a segment of a program, or a part of code, which contains one or more executable instructions for implementing a specified logical function. In some alternative implementations, the functions noted in the blocks may occur in a different order from that noted in the accompanying drawings. For example, two consecutive blocks may in fact be executed substantially in parallel, or they may sometimes be executed in the reverse order, depending on the functions involved. In the descriptions corresponding to the flowcharts and block diagrams in the accompanying drawings, the operations or steps corresponding to different blocks may also occur in a different order from that disclosed in the description, and sometimes there is no specific order between different operations or steps. For example, two consecutive operations or steps may in fact be executed substantially in parallel, or they may sometimes be executed in the reverse order, depending on the functions involved. Each block in the block diagram and / or flowchart, and combinations of blocks in the block diagram and / or flowchart, may be implemented by a dedicated hardware-based system that performs the specified functions or actions, or may be implemented by a combination of dedicated hardware and computer instructions.

Claims

1. A method for determining fishy smell substances, characterized in that, Comprising: Mix the sample and the internal standard 2,4,6-trimethylpyridine, and stir to obtain a mixed sample; Perform headspace extraction on the mixed sample to generate a substance to be measured containing fishy smell substances; Use a gas chromatography-mass spectrometry (GC-MS) instrument to measure the fishy smell substances in the substance to be measured.

2. The method according to claim 1, wherein Mix the sample and the internal standard 2,4,6-trimethylpyridine, including: Mix the sample and the 2,4,6-trimethylpyridine solution in a ratio of 3 - 5 mL:10 μL of the sample to the 2,4,6-trimethylpyridine solution; wherein, the concentration of the 2,4,6-trimethylpyridine solution is 20.00 - 22.00 ppm.

3. The method according to claim 1, characterized in that, Stir, including: Stir at a temperature of 55 - 60 °C for 8 - 10 min.

4. The method according to claim 1, wherein Perform headspace extraction on the mixed sample, including: Perform headspace extraction at a temperature of 55 - 60 °C for 30 - 40 min.

5. The method according to claim 1, wherein Use a gas chromatography-mass spectrometry (GC-MS) instrument to measure the fishy smell substances in the substance to be measured, including: Separate each component in the substance to be measured using a gas chromatograph; Detect each component separately using a mass spectrometer; Process the detection results to obtain the content of the fishy smell substances in the substance to be measured.

6. The method according to claim 5, characterized in that Separate each component in the substance to be measured using a gas chromatograph, including: Under a temperature programming, use a gas chromatograph to extract each component in the substance to be measured at a flow rate of 0.5 - 1.5 mL / min; Wherein, the chromatographic column of the gas chromatograph is a 30 m × 0.25 mm, 0.25 μm elastic capillary column; the carrier gas of the gas chromatograph is helium.

7. The method according to claim 6, wherein The temperature programming, including: The first stage, the chromatographic column is kept at an initial temperature of 40 - 50 °C for 2 - 3 min; The second stage, the temperature of the chromatographic column is increased from the initial temperature to 230 - 240 °C at a heating rate of 8 - 10 °C / min; The third stage, the temperature of the chromatographic column is kept at 230 - 240 °C for 5 - 6 min.

8. The method according to claim 5, characterized in that, Detect each component separately using a mass spectrometer, including: Set the mass spectrometer according to the following parameters: the temperatures of the injection port and the interface are the same, both being 240 - 250 °C; use an electron impact ionization source, the electron energy is 60 - 70 eV, the emission current is 1 - 1.5 mA, the temperature is 200 - 210 °C; the detector voltage is 1700 - 1800 V; the scanning range is 33 - 400 amu.

9. The method according to claim 5, characterized in that Process the detection results to obtain the content of the fishy smell substances in the substance to be measured, including: Automatically retrieve the detection results in the database; Select the measurement results with a purity and a matching degree both greater than 800 in the retrieval results; Perform normalization processing and analysis on the selected measurement results to obtain the content of the fishy smell substances in the substance to be measured.

10. An apparatus for measuring fishy smell substances, comprising a processor and a memory storing program instructions, characterized in that, The processor is configured to execute the method for measuring fishy smell substances according to any one of claims 1 to 9 when running the program instructions.

11. A testing device, comprising a gas chromatography-mass spectrometry instrument, characterized in that, Further comprising: The device for measuring fishy smell substances according to claim 10 is installed on the test equipment.

12. A storage medium stores program instructions, characterized in that, When the program instructions are running, they are used to cause the computer to execute the method for measuring fishy smell substances according to any one of claims 1 to 9.

Images