A seven-clearing and toxin-eliminating granular preparation for poultry and a preparation method thereof

Abstract

The present application relates to the technical field of pharmaceutical preparation, and in particular to a seven-clearing toxin-eliminating granule preparation for poultry and a preparation method thereof.The raw materials of the seven-clearing toxin-eliminating granule preparation for poultry include, by mass fraction, Huangqi 15-30 parts, Huzhang 15-30 parts, Baishouweng 10-20 parts, Kushen 10-20 parts, Banlangen 15-30 parts, Mianma Guanzong 10-20 parts, Dacaiye 5-15 parts, hydroxypropyl-beta-cyclodextrin 10-15 parts, dispersing agent 0.1-1 part, compound enzyme preparation 1-3 parts, Huangqipolysaccharide 1-3 parts, and sucrose 70-100 parts.The present application has no any toxic side effects and successfully solves the industry problems of slow effect and large dosage of traditional Chinese veterinary drugs, and provides reliable technical support for antibiotic-free breeding.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical preparation technology, and in particular to a Qiqing Baidu Granules preparation for poultry and its preparation method. Background Technology

[0002] Chicken farming has evolved from scattered farming to intensive large-scale farming. Currently, due to factors such as management level, feeding conditions, disease prevention level, and climate, conditions for the spread of certain diseases have been created. Sick chicks are weak, have no appetite, and are prone to sudden death, causing serious economic losses.

[0003] The widespread use of chemically synthesized veterinary drugs in poultry farming has led to increasingly prominent issues related to veterinary drug residues in animal products. Domestic and international research indicates that long-term or excessive use of veterinary drugs not only reduces the quality of livestock products and causes significant economic losses, but also harms human health, even posing a serious threat to life—a greater danger than animal diseases to humans.

[0004] Currently, most traditional Chinese veterinary medicines have become an important class of therapeutic drugs in veterinary clinical practice due to their low toxicity and side effects and the low likelihood of residues after long-term use. They are particularly favored in the production of pollution-free livestock products. However, current traditional Chinese medicine preparations generally suffer from low bioavailability and slow drug release, requiring large quantities to achieve the desired effect, resulting in significant drug waste, which urgently needs to be addressed. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a Qiqing Baidu Granules preparation for poultry and its preparation method.

[0006] A Qiqing Baidu Granules preparation for poultry, comprising the following raw materials by weight: 15-30 parts of Scutellaria baicalensis, 15-30 parts of Polygonum cuspidatum, 10-20 parts of Pulsatilla chinensis, 10-20 parts of Sophora flavescens, 15-30 parts of Isatis indigotica, 10-20 parts of Dryopteris crassirhizoma, 5-15 parts of Isatis tinctoria, 10-15 parts of hydroxypropyl-β-cyclodextrin, 0.1-1 part of dispersant, 1-3 parts of compound enzyme preparation, 1-3 parts of Astragalus polysaccharide, and 70-100 parts of sucrose.

[0007] Scutellaria baicalensis, bitter in taste and cold in nature. It enters the lung, gallbladder, spleen, large intestine, and small intestine meridians. It clears heat and dries dampness, drains fire and detoxifies, stops bleeding, and calms the fetus. It is used for damp-heat syndrome, summer-heat syndrome with chest tightness and nausea, damp-heat fullness, diarrhea, jaundice, lung-heat cough, high fever and thirst, blood-heat vomiting and nosebleeds, carbuncles and boils, and threatened abortion.

[0008] Polygonum cuspidatum has a slightly bitter taste and is slightly cold in nature. It enters the liver, gallbladder, and lung meridians. It clears heat and detoxifies, promotes bile secretion and reduces jaundice, dispels wind and dampness, disperses blood stasis and relieves pain, and stops cough and resolves phlegm. It is used for joint pain, damp-heat jaundice, amenorrhea, abdominal masses, cough with excessive phlegm, burns, injuries from falls, carbuncles, and boils.

[0009] Pulsatilla chinensis, bitter in taste and cold in nature. It enters the stomach and large intestine meridians. It clears heat, cools the blood, and detoxifies. It is used for dysentery due to heat toxicity, malaria with chills and fever, epistaxis, and hemorrhoids with bleeding.

[0010] Sophora flavescens has a bitter taste and is cold in nature. It enters the small intestine, liver, kidney, and large intestine meridians. It clears heat and dries dampness, dispels wind and kills parasites. It is used for damp-heat diarrhea, hematochezia, jaundice, dysuria, edema, leukorrhea, vulvar pruritus, scabies, leprosy, itchy skin, and damp-heat sores.

[0011] Isatis root, bitter in taste and cold in nature. It enters the stomach and liver meridians, clearing heat and detoxifying, cooling the blood and relieving sore throat. It is used for febrile diseases with rashes, dark purple tongue, mumps, sore throat, erysipelas, epidemic parotitis, erysipelas, and carbuncles.

[0012] Dryopteris crassirhizoma (Mianma Guanzhong) is bitter in taste and slightly cold in nature. It enters the liver and stomach meridians. It clears heat and detoxifies, and expels parasites. It is used for abdominal pain due to intestinal parasites and sores. Charred Dryopteris crassirhizoma (Mianma Guanzhong) stops bleeding and is used for metrorrhagia.

[0013] Isatis leaf (Daqingye) is bitter and cold in nature. It enters the stomach and heart meridians. It clears heat and detoxifies, cools the blood and eliminates rashes. It is used for febrile diseases invading the nutritive level, high fever with delirium, rashes, jaundice, dysentery, mumps, sore throat, erysipelas, and carbuncles.

[0014] Preferably, the dispersant is polyvinylpyrrolidone.

[0015] Preferably, the compound enzyme preparation includes cellulase and pectinase.

[0016] More preferably, the specific activity of cellulase is 3000-6000 U / g, and the specific activity of pectinase is 2000-4000 U / g.

[0017] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to a mixture of chlorosulfonic acid and dichloroethane, stir in an ice-salt bath for 6-8 hours, neutralize to neutral, dialyze to remove solvent, freeze dry; then add dispersant and water, mix, and cycle homogenize 2-4 times to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly, freeze-crush to a particle size ≤50μm, add compound enzyme preparation, Astragalus polysaccharide, and water, adjust the pH of the system to 4.5-5, enzymatically hydrolyze at 45-50℃ for 1-2 hours, soak in water for 1-2 hours, extract at high temperature for 1-3 hours, and filter; concentrate the filtrate under reduced pressure to obtain a clear extract. S3. Mix the clear extract and pretreated cyclodextrin, stir at 50-60℃ for 1-2 hours, reduce to 33-37℃ and keep warm for 20-40 minutes, stir with sucrose for 20-30 minutes, granulate, and dry.

[0018] Preferably, in S1, the homogenization pressure is 30-40 MPa.

[0019] Preferably, in step S2, the filtrate is concentrated under reduced pressure to a relative density of 1.20-1.25.

[0020] Preferably, in S2, the reduced pressure concentration temperature is 50-60℃ and the vacuum degree is -0.06 to -0.09 MPa.

[0021] Preferably, in step S3, the granules are passed through a 24-mesh sieve.

[0022] Preferably, in step S3, the moisture content is dried to ≤6% and the drying temperature is 60-65℃.

[0023] Beneficial effects: The Qiqing Baidu Granules preparation obtained by this invention achieves a modern upgrade of traditional Chinese veterinary medicine through innovation, and has significant advantages in preventing and treating common poultry diseases such as white diarrhea and damp-heat diarrhea in chicks.

[0024] This invention employs cryogenic pulverization with liquid nitrogen combined with enzymatic hydrolysis technology to create microcracks in the cell walls of medicinal materials, effectively promoting the dissolution of lipid-soluble components such as Polygonum cuspidatum and matrine. Hydroxypropyl-β-cyclodextrin not only significantly improves water solubility, but its internal cavity also provides excellent encapsulation of lipid-soluble drugs. Furthermore, through sulfonation treatment, the introduction of negative charges on the sulfonic acid groups enhances the binding force with alkaline drugs via electrostatic interaction. This not only shortens the time to peak blood concentration but also effectively prolongs the effective concentration, effectively controlling release and reducing drug waste.

[0025] This invention uses only traditional Chinese medicinal materials and contains no antibiotics. It can promote the recovery of feeding and development of chicks after treatment, ensuring their subsequent growth and development, and avoiding problems such as anorexia, slow growth, and weight loss in chicks after treatment. It helps to promote green and antibiotic-free farming and has significant effects compared with existing drugs.

[0026] This invention has no toxic side effects and successfully solves the industry problems of slow onset and large dosage of traditional Chinese veterinary drugs, providing reliable technical support for antibiotic-free farming. Attached Figure Description

[0027] Figure 1 This is a comparison of the inhibition diameters of the daylily preparations obtained in Example 5 and Comparative Examples 1-2 against the test bacteria (Staphylococcus aureus, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, and Proteus).

[0028] Figure 2 The graph shows a comparison of the body weight, duodenal bacterial load, and cecal bacterial load of chickens in the blank group, model group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group, and amoxicillin group. Detailed Implementation

[0029] The present invention will be further explained below with reference to specific embodiments.

[0030] Example 1 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 150g of Scutellaria baicalensis, 150g of Polygonum cuspidatum, 100g of Pulsatilla chinensis, 100g of Sophora flavescens, 150g of Isatis indigotica, 100g of Dryopteris crassirhizoma, 50g of Isatis tinctoria leaf, 100g of hydroxypropyl-β-cyclodextrin, 1g of polyvinylpyrrolidone, 10g of compound enzyme preparation, 10g of Astragalus polysaccharide, and 700g of sucrose.

[0031] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 3000 U / g, and the specific activity of pectinase is 2000 U / g.

[0032] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a mixture of chlorosulfonic acid and dichloroethane, with a weight ratio of 100:25:250. The mixture was stirred in an ice-salt bath for 7 hours, then quenched by slow addition of ice water. The mixture was neutralized to neutrality using sodium bicarbonate. Small molecule impurities were removed by dialysis using a dialysis bag, and the solvent was removed. The mixture was then lyophilized. Polyvinylpyrrolidone and 100g of water were added and mixed. The mixture was then circulated twice in a high-pressure homogenizer at a homogenization pressure of 30MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 2000g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 45℃ for 1 hour. Add the mixture to an extraction tank, cover the herbs with water, and soak for 1 hour. Maintain the temperature at 80℃ for 1 hour. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.20. The concentration temperature is controlled at 50℃ and the vacuum degree is -0.06MPa to obtain a clear extract. S3. Mix the clear paste and pretreated cyclodextrin, stir at 50℃ for 1 hour at a stirring speed of 100 r / min, reduce the temperature to 33℃ and keep warm for 20 minutes, add sucrose to a trough mixer and stir for 20 minutes to form a soft material, add it to a swing granulator, pass it through a 24-mesh sieve to make wet granules, put the wet granules into a drying tray and dry at 60℃ until the moisture content is ≤6%.

[0033] Example 2 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 300g of Scutellaria baicalensis, 300g of Polygonum cuspidatum, 200g of Pulsatilla chinensis, 200g of Sophora flavescens, 300g of Isatis indigotica, 200g of Dryopteris crassirhizoma, 150g of Isatis tinctoria leaf, 150g of hydroxypropyl-β-cyclodextrin, 10g of polyvinylpyrrolidone, 30g of compound enzyme preparation, 30g of Astragalus polysaccharide, and 1000g of sucrose.

[0034] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 6000 U / g, and the specific activity of pectinase is 4000 U / g.

[0035] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a mixture of chlorosulfonic acid and dichloroethane at a weight ratio of 150:20:200. The mixture was stirred in an ice-salt bath for 8 hours, quenched by slow dropwise addition of ice water, and neutralized to neutral with sodium bicarbonate. Small molecule impurities were removed by dialysis using a dialysis bag, the solvent was removed, and the mixture was lyophilized. Polyvinylpyrrolidone and 300g of water were then added and mixed. The mixture was then circulated four times in a high-pressure homogenizer at a homogenization pressure of 40MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 4000g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 50℃ for 2 hours. Add the mixture to an extraction tank, cover the herbs with water, and soak for 2 hours. Maintain the temperature at 90℃ for 3 hours. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.25. The concentration temperature is controlled at 60℃ and the vacuum degree is -0.07MPa to obtain a clear extract. S3. Mix the clear paste and pretreated cyclodextrin, stir at 60℃ for 2 hours at a stirring speed of 300r / min, then reduce the temperature to 37℃ and keep warm for 40 minutes. Add the sucrose to a trough mixer and stir for 30 minutes to form a soft mass. Add the soft mass to a swing granulator and pass it through a 24-mesh sieve to make wet granules. Place the wet granules in a drying tray and dry them at 65℃ until the moisture content is ≤6%.

[0036] Example 3 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 200g of Scutellaria baicalensis, 240g of Polygonum cuspidatum, 120g of Pulsatilla chinensis, 160g of Sophora flavescens, 200g of Isatis indigotica, 180g of Dryopteris crassirhizoma, 80g of Isatis tinctoria leaf, 130g of hydroxypropyl-β-cyclodextrin, 7g of polyvinylpyrrolidone, 15g of compound enzyme preparation, 25g of Astragalus polysaccharide, and 800g of sucrose.

[0037] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 5000 U / g, and the specific activity of pectinase is 2500 U / g.

[0038] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a mixture of chlorosulfonic acid and dichloroethane, with a weight ratio of 130:20:200. The mixture was stirred in an ice-salt bath for 6 hours, then quenched by slow dropwise addition of ice water. The mixture was neutralized to neutrality using sodium bicarbonate. Small molecule impurities were removed by dialysis using a dialysis bag, and the solvent was removed. The mixture was then lyophilized. Polyvinylpyrrolidone and 250g of water were added and mixed. The mixture was then circulated three times in a high-pressure homogenizer at a homogenization pressure of 33MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 3400g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 46℃ for 100min. Add the mixture to an extraction tank, cover the herbs with water, and soak for 80min. Maintain the temperature at 85℃ for 2.5h. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.21. The concentration temperature is controlled at 58℃ and the vacuum degree is -0.08MPa to obtain a clear extract. S3. Mix the clear paste and pretreated cyclodextrin, stir at 58℃ for 80 minutes at a stirring speed of 250 r / min, reduce the temperature to 33℃ and keep warm for 35 minutes, add sucrose to a trough mixer and stir for 22 minutes to form a soft material, add it to a swing granulator, pass it through a 24-mesh sieve to make wet granules, put the wet granules into a drying tray and dry at 63℃ until the moisture content is ≤6%.

[0039] Example 4 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 240g of Scutellaria baicalensis, 200g of Polygonum cuspidatum, 180g of Pulsatilla chinensis, 120g of Sophora flavescens, 280g of Isatis indigotica, 140g of Dryopteris crassirhizoma, 120g of Isatis tinctoria leaf, 110g of hydroxypropyl-β-cyclodextrin, 3g of polyvinylpyrrolidone, 25g of compound enzyme preparation, 15g of Astragalus polysaccharide, and 900g of sucrose.

[0040] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 4000 U / g, and the specific activity of pectinase is 3500 U / g.

[0041] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a mixture of chlorosulfonic acid and dichloroethane, with a weight ratio of 110:20:200. The mixture was stirred in an ice-salt bath for 6 hours, then quenched by slow dropwise addition of ice water. The mixture was neutralized to neutrality using sodium bicarbonate. Small molecule impurities were removed by dialysis using a dialysis bag, and the solvent was removed. The mixture was then lyophilized. Polyvinylpyrrolidone and 150g of water were added and mixed. The mixture was then circulated three times in a high-pressure homogenizer at a homogenization pressure of 37MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 2600g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 48℃ for 80min. Add the mixture to an extraction tank, cover the herbs with water, and soak for 100min. Maintain the temperature at 85℃ for 1.5h. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.23. The concentration temperature is controlled at 55℃ and the vacuum degree is -0.085MPa to obtain a clear extract. S3. Mix the clear extract and pretreated cyclodextrin, stir at 52℃ for 100 min at a stirring speed of 150 r / min, then reduce the temperature to 37℃ and keep warm for 25 min. Add the sucrose to a trough mixer and stir for 28 min to form a soft mass. Add the soft mass to a swing granulator and pass it through a 24-mesh sieve to form wet granules. Place the wet granules in a drying tray and dry them at 61℃ until the moisture content is ≤6%.

[0042] Example 5 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 220g of Scutellaria baicalensis, 220g of Polygonum cuspidatum, 150g of Pulsatilla chinensis, 150g of Sophora flavescens, 240g of Isatis indigotica, 150g of Dryopteris crassirhizoma, 100g of Isatis tinctoria leaf, 120g of hydroxypropyl-β-cyclodextrin, 5g of polyvinylpyrrolidone, 20g of compound enzyme preparation, 20g of Astragalus polysaccharide, and 850g of sucrose.

[0043] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 4500 U / g, and the specific activity of pectinase is 3000 U / g.

[0044] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a mixture of chlorosulfonic acid and dichloroethane at a weight ratio of 120:20:200. The mixture was stirred in an ice-salt bath for 7 hours, then quenched by slow addition of ice water. The mixture was neutralized to neutrality using sodium bicarbonate. Small molecule impurities were removed by dialysis using a dialysis bag. The solvent was removed and the mixture was lyophilized. Polyvinylpyrrolidone and 200g of water were then added and mixed. The mixture was then circulated three times in a high-pressure homogenizer at a homogenization pressure of 35MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 3000g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 47℃ for 90min. Add the mixture to an extraction tank, cover the herbs with water, and soak for 90min. Maintain the temperature at 90℃ for 2h. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.22. The concentration temperature is controlled at 55℃ and the vacuum degree is -0.085MPa to obtain a clear extract. S3. Mix the clear paste and pretreated cyclodextrin, stir at 55℃ for 90 minutes at a stirring speed of 200 r / min, then reduce the temperature to 35℃ and keep warm for 30 minutes. Add the sucrose to a trough mixer and stir for 25 minutes to form a soft mass. Add the soft mass to a swing granulator and pass it through a 24-mesh sieve to form wet granules. Place the wet granules in a drying tray and dry them at 62℃ until the moisture content is ≤6%.

[0045] Comparative Example 1 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 220g of Scutellaria baicalensis, 220g of Polygonum cuspidatum, 150g of Pulsatilla chinensis, 150g of Sophora flavescens, 240g of Isatis indigotica, 150g of Dryopteris crassirhizoma, 100g of Isatis tinctoria leaf, 120g of hydroxypropyl-β-cyclodextrin, 5g of polyvinylpyrrolidone, 20g of compound enzyme preparation, 20g of Astragalus polysaccharide, and 850g of sucrose.

[0046] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 4500 U / g, and the specific activity of pectinase is 3000 U / g.

[0047] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Mix hydroxypropyl-β-cyclodextrin, polyvinylpyrrolidone and 200g of water, and send the mixture into a high-pressure homogenizer for three cycles of homogenization at a pressure of 35MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 3000g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 47℃ for 90min. Add the mixture to an extraction tank, cover the herbs with water, and soak for 90min. Maintain the temperature at 90℃ for 2h. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.22. The concentration temperature is controlled at 55℃ and the vacuum degree is -0.085MPa to obtain a clear extract. S3. Mix the clear paste and pretreated cyclodextrin, stir at 55℃ for 90 minutes at a stirring speed of 200 r / min, then reduce the temperature to 35℃ and keep warm for 30 minutes. Add the sucrose to a trough mixer and stir for 25 minutes to form a soft mass. Add the soft mass to a swing granulator and pass it through a 24-mesh sieve to form wet granules. Place the wet granules in a drying tray and dry them at 62℃ until the moisture content is ≤6%.

[0048] Comparative Example 2 A Qiqing Baidu Granules preparation for poultry, the raw materials of which include: 220g of Scutellaria baicalensis, 220g of Lonicera japonica, 150g of Pulsatilla chinensis, 150g of Sophora flavescens, 240g of Isatis indigotica, 150g of Dryopteris crassirhizoma, 100g of Isatis tinctoria leaf, 120g of hydroxypropyl-β-cyclodextrin, 5g of polyvinylpyrrolidone, 20g of compound enzyme preparation, 20g of Astragalus polysaccharide, and 850g of sucrose.

[0049] The compound enzyme preparation consists of cellulase and pectinase; the specific activity of cellulase is 4500 U / g, and the specific activity of pectinase is 3000 U / g.

[0050] The preparation method of the above-mentioned Qiqing Baidu Granules for poultry includes the following steps: S1. Hydroxypropyl-β-cyclodextrin was added to a mixture of chlorosulfonic acid and dichloroethane at a weight ratio of 120:20:200. The mixture was stirred in an ice-salt bath for 7 hours, then quenched by slow addition of ice water. The mixture was neutralized to neutrality using sodium bicarbonate. Small molecule impurities were removed by dialysis using a dialysis bag. The solvent was removed and the mixture was lyophilized. Polyvinylpyrrolidone and 200g of water were then added and mixed. The mixture was then circulated three times in a high-pressure homogenizer at a homogenization pressure of 35MPa to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Lonicera japonica, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly. Cryogenically pulverize the mixture with liquid nitrogen until the particle size is ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and 3000g of water. Adjust the pH of the system to 4.5-5. Enzymatically hydrolyze the mixture at 47℃ for 90min. Add the mixture to an extraction tank, cover the herbs with water, and soak for 90min. Maintain the temperature at 90℃ for 2h. Filter the decocted liquid through a 200-mesh filter. Concentrate the filtrate under reduced pressure to a relative density of 1.22. The concentration temperature is controlled at 55℃ and the vacuum degree is -0.085MPa to obtain a clear extract. S3. Mix the clear paste and pretreated cyclodextrin, stir at 55℃ for 90 minutes at a stirring speed of 200 r / min, then reduce the temperature to 35℃ and keep warm for 30 minutes. Add the sucrose to a trough mixer and stir for 25 minutes to form a soft mass. Add the soft mass to a swing granulator and pass it through a 24-mesh sieve to form wet granules. Place the wet granules in a drying tray and dry them at 62℃ until the moisture content is ≤6%.

[0051] The granule preparations obtained in Example 5 and Comparative Examples 1-2 were used for in vitro antibacterial tests, as follows: Staphylococcus aureus, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, and Proteus were inoculated separately, and 5 mm wells were punched in each agar plate. The Qiqing Baidu granule preparations obtained in Example 5 and Comparative Examples 1-2 were diluted 100 times and added to the wells of each agar plate. The plates were incubated at 37°C for 24 h, and the diameter of the drug inhibition on each agar plate in each group was counted.

[0052] like Figure 1 As shown, the Qiqing Baidu Granules preparations obtained in Example 5 and Comparative Examples 1-2 all exhibited varying degrees of antibacterial activity against Staphylococcus aureus, Shigella dysenteriae, Pseudomonas aeruginosa, Escherichia coli, and Proteus vulgaris; while the Qiqing Baidu Granules preparation obtained in Example 5 showed the strongest antibacterial effect against each strain, which was superior to that of Comparative Examples 1-2 (P < 0.05).

[0053] Animal experiments were conducted using the chrysanthemum preparations obtained in Example 5 and Comparative Examples 1-2. Hy-Line Brown roosters were used as experimental subjects. 180 one-day-old Hy-Line Brown roosters were raised to seven days of age and, when they were in good growth condition, were randomly divided into six groups: a control group, a model group, the Example 5 group, Comparative Example 1 group, Comparative Example 2 group, and an amoxicillin group. Each rooster in the control group was administered 0.2 mL of physiological saline by gavage, while each rooster in the other groups was administered 0.2 mL of bacterial suspension (concentration 2.5 × 10⁻⁶) by gavage. 8 Modeling was performed using CFU / 0.2mL. Subsequently, groups 5 (Example 5), 1 (Comparative Example 1), and 2 (Comparative Example 2) were administered the drug via gavage (5g of product added per 1L of water). The amoxicillin group was administered amoxicillin soluble powder via gavage (dosage dose of 0.4g / kg), while the model group was administered an equal volume of distilled water via gavage. All groups were administered the drug once a day for a total of 5 days. During the experiment, each group had free access to food and water. After drug withdrawal, the group was observed for another 5 days. The chickens in each group were weighed, then euthanized and dissected. The contents of the duodenum and cecum were collected, and the bacterial load was detected (the bacterial load in the blank group was set to 0).

[0054] like Figure 2 As shown, the blank group had the highest body weight, with no significant difference from the Example 5 group, while the body weight of both the blank group and the Example 5 group was better than that of the other groups (P < 0.05); the amoxicillin group had the lowest duodenal bacterial load, with no significant difference from the Example 5 group, while the duodenal bacterial load of both the amoxicillin group and the Example 5 group was better than that of the other groups (P < 0.05); the Example 5 group had the lowest cecal bacterial load, which was better than that of the other groups (P < 0.05).

[0055] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A Qiqing Baidu Granules preparation for poultry, characterized in that, The raw materials, by weight, include: 15-30 parts of Scutellaria baicalensis, 15-30 parts of Polygonum cuspidatum, 10-20 parts of Pulsatilla chinensis, 10-20 parts of Sophora flavescens, 15-30 parts of Isatis indigotica, 10-20 parts of Dryopteris crassirhizoma, 5-15 parts of Isatis tinctoria, 10-15 parts of hydroxypropyl-β-cyclodextrin, 0.1-1 parts of dispersant, 1-3 parts of compound enzyme preparation, 1-3 parts of Astragalus polysaccharide, and 70-100 parts of sucrose. The compound enzyme preparation includes: cellulase and pectinase; the specific activity of cellulase is 3000-6000 U / g, and the specific activity of pectinase is 2000-4000 U / g; The following steps are used to prepare it: S1. Add hydroxypropyl-β-cyclodextrin to a mixture of chlorosulfonic acid and dichloroethane, stir in an ice-salt bath for 6-8 hours, neutralize to neutral, dialyze to remove solvent, freeze dry; then add dispersant and water, mix, and cycle homogenize 2-4 times to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly, and use liquid nitrogen to freeze-crush the mixture to a particle size ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and water, adjust the pH of the system to 4.5-5, enzymatically hydrolyze at 45-50℃ for 1-2 hours, soak in water for 1-2 hours, extract for 1-3 hours, and filter. Concentrate the filtrate under reduced pressure to obtain a clear extract. S3. Mix the clear extract and pretreated cyclodextrin, stir at 50-60℃ for 1-2 hours, reduce to 33-37℃ and keep warm for 20-40 minutes, stir with sucrose for 20-30 minutes, granulate, and dry.

2. The Qiqing Baidu Granules preparation for poultry according to claim 1, characterized in that, The dispersant is polyvinylpyrrolidone.

3. A method for preparing a Qiqing Baidu Granules formulation for poultry as described in any one of claims 1-2, characterized in that, Includes the following steps: S1. Add hydroxypropyl-β-cyclodextrin to a mixture of chlorosulfonic acid and dichloroethane, stir in an ice-salt bath for 6-8 hours, neutralize to neutral, dialyze to remove solvent, freeze dry; then add dispersant and water, mix, and cycle homogenize 2-4 times to obtain pretreated cyclodextrin. S2. Mix Scutellaria baicalensis, Polygonum cuspidatum, Pulsatilla chinensis, Sophora flavescens, Isatis indigotica, Dryopteris crassirhizoma, and Isatis tinctoria leaves evenly, and use liquid nitrogen to freeze-crush the mixture to a particle size ≤50μm. Add compound enzyme preparation, Astragalus polysaccharide, and water, adjust the pH of the system to 4.5-5, enzymatically hydrolyze at 45-50℃ for 1-2 hours, soak in water for 1-2 hours, extract for 1-3 hours, and filter. Concentrate the filtrate under reduced pressure to obtain a clear extract. S3. Mix the clear extract and pretreated cyclodextrin, stir at 50-60℃ for 1-2 hours, reduce to 33-37℃ and keep warm for 20-40 minutes, stir with sucrose for 20-30 minutes, granulate, and dry.

4. The method for preparing the Qiqing Baidu Granules for poultry according to claim 3, characterized in that, In S1, the homogenization pressure is 30-40 MPa.

5. The method for preparing the Qiqing Baidu Granules for poultry according to claim 3, characterized in that, In S2, the filtrate is concentrated under reduced pressure to a relative density of 1.20-1.

25.

6. The method for preparing the Qiqing Baidu Granules for poultry according to claim 3, characterized in that, In S2, the reduced pressure concentration temperature is 50-60℃, and the vacuum degree is -0.06 to -0.09 MPa.

7. The method for preparing the Qiqing Baidu Granules for poultry according to claim 3, characterized in that, In S3, the pellets are passed through a 24-mesh sieve after granulation.

8. The method for preparing the Qiqing Baidu Granules for poultry according to claim 3, characterized in that, In S3, the moisture content is dried to ≤6% at a temperature of 60-65℃.

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