Compound bacterium enzyme synergistic fertilizer composition and preparation method thereof

Abstract

The invention relates to the technical field of biological fertilizers, in particular to a compound bacterium and enzyme synergistic fertilizer composition and a preparation method thereof.The preparation method of the compound bacterium and enzyme synergistic fertilizer composition comprises the steps that 1, livestock manure and mushroom residues are mixed, crushed corn straw is added to adjust the carbon nitrogen ratio, and a fermentation raw material is obtained; step 2, adding laccase into the fermentation raw material, inoculating a complex microbial inoculant, and carrying out aerobic fermentation to obtain a primary composted material; step 3, adding a compound enzyme agent into the primary composted material, inoculating clostridium butyricum, and performing anaerobic fermentation to obtain a final composted material; and step 4, mixing the final composted material with an adsorption carrier, adding the composite stabilizing solution, uniformly stirring, drying at low temperature, and granulating to obtain the composite bacterium-enzyme synergistic fertilizer composition. The prepared fertilizer composition can significantly improve the plant height, stem diameter, disc diameter and other key growth indexes of sunflowers, effectively reduce the empty shell rate, greatly improve the single disc weight and acre yield, and significantly enhance the fertilizer utilization rate.

Description

Technical Field

[0001] The present application relates to the technical field of biological fertilizers, and more specifically, to a composite bacterial enzyme synergistic fertilizer composition and a preparation method thereof. Background Art

[0002] The global soil microbial diversity is facing a severe situation of rapid decline. Long-term and large-scale use of chemical fertilizers has led to a significant reduction in the abundance of beneficial bacterial communities (such as nitrogen-fixing bacteria, phosphorus-solubilizing bacteria, etc.) in the soil, the structure of the microbial community has been damaged, and the activities of soil enzymes (such as urease, sucrase, etc.) have also decreased significantly. Although traditional microbial fertilizers can partially supplement functional bacteria, the colonization rate of single strains in the soil is relatively low, and they are easily inactivated under the stress of factors such as soil environment and heavy metals. In existing enzyme fertilizers, the half-life of enzymes such as cellulase and protease in the soil is relatively short, and nutrients need to be further converted into absorbable nutrients by microorganisms, resulting in a lag in nutrient release.

[0003] However, existing bacterial enzyme compound fertilizers generally have problems of functional antagonism. Organic acids (such as lactic acid) produced by microbial metabolism may reduce the activity of enzymes, while free amino acids released during the enzymatic hydrolysis process will inhibit the proliferation of some functional bacteria. In addition, there are problems such as mismatched action time sequences of bacteria and enzymes, resulting in low synergy efficiency and limited improvement in fertilizer efficiency. Based on the above situation, the present application provides a composite bacterial enzyme synergistic fertilizer composition and a preparation method thereof, which are used to improve soil fertilizer efficiency, promote plant growth, while reducing the use amount of chemical fertilizers and realizing the sustainable development of agriculture. Summary of the Invention

[0004] In order to solve the technical problems mentioned in the background art, the present application provides a composite bacterial enzyme synergistic fertilizer composition and a preparation method thereof.

[0005] A preparation method of a composite bacterial enzyme synergistic fertilizer composition includes the following steps: Step 1: Mix livestock and poultry manure and mushroom residue to obtain a mixture, add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25 - 30:1, control the pH to 6.5 - 8.0, and the water content to 60 - 70% to obtain a fermentation raw material; Step 2: Add laccase and inoculate a composite bacterial agent to the fermentation raw material, and carry out aerobic fermentation at 25 - 37°C, control the ventilation volume per kilogram of fermentation raw material to 0.4 - 0.6 L / min, and continuously ferment for 1 - 2 days to obtain a primary matured material; Step 3: Add the primary matured material to a sealed fermentation tank, add a composite enzyme agent and inoculate Clostridium butyricum, and carry out anaerobic fermentation, control the fermentation temperature to 25 - 32°C, and ferment for 5 - 7 days to obtain a final matured material; Step 4: Mix the final compost and the adsorption carrier, add the composite stabilizing solution, stir evenly, then dry at low temperature and granulate to obtain the composite enzyme and bacteria synergistic fertilizer composition.

[0006] Preferably, in the step 1, the mass ratio of the livestock and poultry manure to the mushroom residue is 3 - 5:1 - 2.

[0007] Preferably, in the step 2, the addition amount of laccase is 0.5 - 1% of the fermentation raw material.

[0008] Preferably, in the step 2, the inoculation amount of the composite bacterium agent is 3 - 8% of the fermentation raw material; Preferably, the composite bacterium agent in the step 2 is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable count ratio of 8 - 12:1 - 3:2 - 4.

[0009] Preferably, in the step 3, the addition amount of the composite enzyme agent is 0.5 - 2% of the primary compost.

[0010] Preferably, the composite enzyme agent in the step 3 is composed of xylanase, papain and phytase with a mass ratio of 20 - 30:5 - 12:3 - 8.

[0011] Preferably, in the step 3, the inoculation amount of Clostridium butyricum is 3 - 5% of the primary compost.

[0012] Preferably, in the step 4, the mass ratio of the final compost, the adsorption carrier and the composite stabilizing solution is 10 - 12:15 - 35:20 - 35.

[0013] Preferably, the adsorption carrier in the step 4 is one or more of zeolite powder, rice chaff, diatomite, vermiculite.

[0014] Preferably, the composite stabilizing solution in the step 4 is composed of sodium lignosulfonate, pullulan polysaccharide, polyglutamic acid and deionized water with a mass ratio of 1 - 3:1 - 2:2 - 5:80 - 120.

[0015] A composite enzyme and bacteria synergistic fertilizer composition is prepared by the above method.

[0016] In summary, the present application has the following beneficial effects: In the process of preparing the fermentation raw material in the present application, by mixing the livestock and poultry manure and the mushroom residue in a certain proportion, and adjusting the carbon-nitrogen ratio with corn straw fragments, the manure provides a nitrogen source and organic matter, and the mushroom residue contains residual mycelia and can be used as a microbial nutrient source. The addition of the mushroom residue and corn straw fragments can increase the porosity, improve the air permeability, reduce the risk of pile adhesion, and facilitate the colonization of aerobic microorganisms.

[0017] In this application, laccase and a compound microbial agent are added to the fermentation raw materials for aerobic fermentation treatment. First, laccase specifically decomposes lignin, releasing cellulose and hemicellulose, improving the utilization rate of organic matter and shortening the composting time. Laccase can also catalyze the oxidation of phenolic substances to form quinone compounds, which have high reactivity and can inhibit the growth of pathogenic bacteria (such as Escherichia coli and Salmonella) and reduce ammonia volatilization. Adding it in the initial stage of fermentation can not only shorten the composting time but also inhibit the generation of pathogenic bacteria and promote the colonization of compound strains. Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum are selected for compound fermentation. They can not only quickly decompose proteins and starches to produce small molecule peptides and sugars, but the antibacterial substances produced by Bacillus subtilis can inhibit the growth of spoilage bacteria. In addition, Trichoderma harzianum can parasitize pathogenic fungi to induce plant disease resistance, forming an "antibacterial-disease prevention" synergistic system with Bacillus subtilis and Lactobacillus pentosus.

[0018] Through the directional decomposition of the compound enzyme preparation in this application, xylanase degrades hemicellulose in straw into monosaccharides, increasing soluble carbon sources and providing carbon sources for Clostridium butyricum. Papain decomposes uncomposted proteins into amino acids, increasing the quick-acting property of the fertilizer. Phytase activates insoluble phosphates in the soil, mineralizes organic phosphorus into a form available to plants, and increases the phosphorus content. The compound enzyme preparation precisely degrades the complex organic matter remaining in the primary composted material. The butyric acid produced by the metabolism of Clostridium butyricum and the lactic acid produced by the fermentation of Lactobacillus pentosus play a synergistic role, which can regulate the soil microbial community, promote plant root development, improve the quick-acting property of the fertilizer, and enhance the stress resistance of crops. This application first uses the synergy of laccase and the compound microbial agent in an aerobic environment to convert solid organic materials into soluble small molecules (such as reducing sugars and amino acids), providing high-quality carbon and nitrogen sources for anaerobic fermentation. Then, through the combination of an anaerobic environment and anaerobic bacteria, directional conversion of nutrients (such as the conversion of organic phosphorus into inorganic phosphorus) is achieved, and biologically active metabolites (such as butyric acid) are accumulated. This staged synergistic strategy enables the fertilizer to have functions such as supplying quick-acting nutrients, maintaining long-term fertility, and improving soil health. Mixing the ultimate composted material with an adsorption carrier increases the porosity, improves the water retention and air permeability of the fertilizer, avoids hardening, and extends the fertilizer efficiency. The compound stabilizing solution is prepared by compounding sodium lignosulfonate, pullulan, and polyglutamic acid, and the three form a stable network structure to achieve long-term slow release of nutrients and microbial protection. Detailed implementation manners

[0019] The following further elaborates on this application with reference to examples.

[0020] In the examples and comparative examples of this application, Bacillus subtilis (product number: B98052), Trichoderma harzianum (product number: B81740), Lactobacillus pentosus (product number: B81252), and Clostridium butyricum (product number: BMZ109220) were all purchased from Ningbo Mingzhou Biotechnology Co., Ltd.; laccase (enzyme activity: 10,000) was purchased from Jiangsu Enming Bioengineering Technology Co., Ltd.; xylanase (enzyme activity: 100,000) was purchased from Hubei Haijia Biotechnology Co., Ltd.; papain (enzyme activity: 100,000) was purchased from Shandong Fangchang Biotechnology Co., Ltd.; phytase (enzyme activity: 10,000) was purchased from Hebei Jiuyu Biotechnology Co., Ltd.; sodium lignosulfonate was purchased from Shandong Jinrong Chemical Technology Co., Ltd.; pullulan polysaccharide was purchased from Nanjing Baiju Technology Co., Ltd.; polyglutamic acid (product number: GF110) was purchased from Jinan Guofeng Chemical Co., Ltd.

[0021] Examples 1-3 and Comparative Examples 1-7 provide a compound bacterial enzyme synergistic fertilizer composition and a preparation method thereof.

[0022] Example 1 A preparation method of a compound bacterial enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue in a mass ratio of 3:1 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to 6.5, and the water content to 60% to obtain a fermentation raw material. Step 2: Add laccase to the fermentation raw material and inoculate with a compound bacterial agent. Conduct aerobic fermentation at 25°C, control the ventilation volume per kilogram of fermentation raw material to 0.4 L / min, and continuously ferment for 1 day to obtain a primary compost. Among them, the addition amount of laccase is 0.5% of the fermentation raw material, and the inoculation amount of the compound bacterial agent is 3% of the fermentation raw material; the compound bacterial agent is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 8:1:2. Step 3: Add the primary compost to a sealed fermentation tank, add a compound enzyme agent and inoculate with Clostridium butyricum, and conduct anaerobic fermentation. Control the fermentation temperature to 25°C and ferment for 5 days to obtain a final compost. Among them, the addition amount of the compound enzyme agent is 0.5% of the primary compost, the compound enzyme agent is composed of xylanase, papain, and phytase in a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary compost. Step 4: Mix the final compost and rice hulls, add a compound stabilizing solution, stir at a speed of 100 rpm for 20 min. After stirring evenly, dry at a low temperature of 40°C for 12 h and granulate to obtain a compound bacterial enzyme synergistic fertilizer composition. Among them, the mass ratio of the final compost, rice hulls, and the compound stabilizing solution is 10:15:20, and the compound stabilizing solution is composed of sodium lignosulfonate, pullulan polysaccharide, polyglutamic acid, and deionized water in a mass ratio of 1:1:2:80.

[0023] Example 2 A preparation method of a compound enzyme synergistic fertilizer composition, comprising the following preparation steps: Step 1: Mix cow dung and mushroom residue according to a mass ratio of 4:1.5 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 28:1, control the pH to 7.2, and the water content to 650% to obtain a fermentation raw material; Step 2: Add laccase to the fermentation raw material and inoculate with a compound bacterial agent. Carry out aerobic fermentation at 32°C, control the ventilation volume per kilogram of fermentation raw material to 0.5 L / min, and continuously ferment for 1.5 days to obtain a primary matured material. Among them, the addition amount of laccase is 0.8% of the fermentation raw material, and the inoculation amount of the compound bacterial agent is 5% of the fermentation raw material; the compound bacterial agent is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 10:2:3; Step 3: Add the primary matured material to a sealed fermentation tank, add a compound enzyme agent and inoculate with Clostridium butyricum, and carry out anaerobic fermentation. Control the fermentation temperature to 28°C and ferment for 6 days to obtain a final matured material. Among them, the addition amount of the compound enzyme agent is 1% of the primary matured material, the compound enzyme agent is composed of xylanase, papain, and phytase with a mass ratio of 25:8:5, and the inoculation amount of Clostridium butyricum is 4% of the primary matured material; Step 4: Mix the final matured material and rice hulls, add a compound stabilizing solution, stir at a rotation speed of 150 rpm for 25 min. After stirring evenly, dry at a low temperature of 45°C for 18 h and granulate to obtain the compound enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice hulls, and the compound stabilizing solution is 11:25:28, and the compound stabilizing solution is composed of sodium lignosulfonate, pullulan polysaccharide, polyglutamic acid, and deionized water with a mass ratio of 2:1.5:4:100.

[0024] Example 3 A preparation method of a compound enzyme synergistic fertilizer composition, comprising the following preparation steps: Step 1: Mix cow dung and mushroom residue according to a mass ratio of 5:2 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 30:1, control the pH to 8.0, and the water content to 70% to obtain a fermentation raw material; Step 2: Add laccase to the fermentation raw material and inoculate with a compound bacterial agent. Carry out aerobic fermentation at 37°C, control the ventilation volume per kilogram of fermentation raw material to 0.6 L / min, and continuously ferment for 2 days to obtain a primary matured material. Among them, the addition amount of laccase is 1% of the fermentation raw material, and the inoculation amount of the compound bacterial agent is 8% of the fermentation raw material; the compound bacterial agent is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 12:3:4; Step 3: Add the primary matured material into a sealed fermentation tank, add a compound enzyme agent and inoculate Clostridium butyricum, and conduct anaerobic fermentation. Control the fermentation temperature at 32 °C and ferment for 7 days to obtain the final matured material. Among them, the addition amount of the compound enzyme agent is 2% of the primary matured material. The compound enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 30:12:8, and the inoculation amount of Clostridium butyricum is 5% of the primary matured material; Step 4: Mix the final matured material and rice bran, add a compound stabilizing solution, stir at a rotation speed of 200 rpm for 30 min. After stirring evenly, conduct low-temperature drying at 50 °C for 24 h and granulate to obtain the compound bacteria-enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice bran and the compound stabilizing solution is 12:35:35. The compound stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 3:2:5:120.

[0025] Comparative Example 1 A preparation method of a compound bacteria-enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH at 6.5, and the water content at 60% to obtain the fermentation raw material; Step 2: Inoculate the compound bacteria agent into the fermentation raw material and conduct aerobic fermentation at 25 °C. Control the ventilation volume per kilogram of the fermentation raw material at 0.4 L / min and continuously ferment for 1 day to obtain the primary matured material. Among them, the inoculation amount of the compound bacteria agent is 3% of the fermentation raw material; the compound bacteria agent is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable count ratio of 8:1:2; Step 3: Add the primary matured material into a sealed fermentation tank, add a compound enzyme agent and inoculate Clostridium butyricum, and conduct anaerobic fermentation. Control the fermentation temperature at 25 °C and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the compound enzyme agent is 0.5% of the primary matured material. The compound enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary matured material; Step 4: Mix the final matured material and rice bran, add a compound stabilizing solution, stir at a rotation speed of 100 rpm for 20 min. After stirring evenly, conduct low-temperature drying at 40 °C for 12 h and granulate to obtain the compound bacteria-enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice bran and the compound stabilizing solution is 10:15:20. The compound stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0026] Comparative Example 2 A preparation method of a compound bacteria-enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue in a mass ratio of 3:1 to obtain a mixture. Add crushed corn straw to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to 6.5, and the water content to 60% to obtain the fermentation raw material. Step 2: Add laccase to the fermentation raw material and inoculate with a composite microbial agent. Conduct aerobic fermentation at 25°C, control the ventilation volume per kilogram of fermentation raw material to be 0.4 L / min, and continuously ferment for 1 day to obtain the primary matured material. Among them, the addition amount of laccase is 0.5% of the fermentation raw material, and the inoculation amount of the composite microbial agent is 3% of the fermentation raw material; the composite microbial agent is composed of Bacillus subtilis and Trichoderma harzianum with an effective viable count ratio of 8:2. Step 3: Add the primary matured material to a sealed fermentation tank, add a composite enzyme agent and inoculate with Clostridium butyricum, conduct anaerobic fermentation, control the fermentation temperature to 25°C, and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the composite enzyme agent is 0.5% of the primary matured material, the composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary matured material. Step 4: Mix the final matured material and rice hulls, add a composite stabilizing solution, stir at a speed of 100 rpm for 20 min. After stirring evenly, dry at a low temperature of 40°C for 12 h and granulate to obtain the composite microbial enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice hulls and the composite stabilizing solution is 10:15:20, and the composite stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0027] Comparative Example 3 A preparation method of a composite microbial enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue in a mass ratio of 3:1 to obtain a mixture. Add crushed corn straw to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to 6.5, and the water content to 60% to obtain the fermentation raw material. Step 2: Add laccase to the fermentation raw material and inoculate with a composite microbial agent. Conduct aerobic fermentation at 25°C, control the ventilation volume per kilogram of fermentation raw material to be 0.4 L / min, and continuously ferment for 1 day to obtain the primary matured material. Among them, the addition amount of laccase is 0.5% of the fermentation raw material, and the inoculation amount of the composite microbial agent is 3% of the fermentation raw material; the composite microbial agent is composed of Bacillus subtilis and Lactobacillus pentosus with an effective viable count ratio of 8:1. Step 3: Add the primary matured material into a sealed fermentation tank, add a composite enzyme agent and inoculate Clostridium butyricum, and carry out anaerobic fermentation. Control the fermentation temperature at 25°C and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the composite enzyme agent is 0.5% of the primary matured material. The composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary matured material; Step 4: Mix the final matured material and rice chaff, add a composite stabilizing liquid, stir at a rotation speed of 100 rpm for 20 min. After stirring evenly, carry out low-temperature drying at 40°C for 12 h and granulate to obtain the composite bacterial enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice chaff and the composite stabilizing liquid is 10:15:20. The composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0028] Comparative Example 4 A preparation method of a composite bacterial enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH at 6.5, and the water content at 60% to obtain a fermentation raw material; Step 2: Add laccase to the fermentation raw material and inoculate a composite bacterial agent, and carry out aerobic fermentation at 25°C. Control the ventilation volume per kilogram of the fermentation raw material at 0.4 L / min and continuously ferment for 1 day to obtain the primary matured material. Among them, the addition amount of laccase is 0.5% of the fermentation raw material, and the inoculation amount of the composite bacterial agent is 3% of the fermentation raw material; the composite bacterial agent is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable count ratio of 8:1:2; Step 3: Add the primary matured material into a sealed fermentation tank, add a composite enzyme agent, and carry out anaerobic fermentation. Control the fermentation temperature at 25°C and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the composite enzyme agent is 0.5% of the primary matured material. The composite enzyme agent is composed of xylanase, papain and phytase with a mass ratio of 20:5:3; Step 4: Mix the final matured material and rice chaff, add a composite stabilizing liquid, stir at a rotation speed of 100 rpm for 20 min. After stirring evenly, carry out low-temperature drying at 40°C for 12 h and granulate to obtain the composite bacterial enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice chaff and the composite stabilizing liquid is 10:15:20. The composite stabilizing liquid is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0029] Comparative Example 5 A preparation method of a composite bacterial enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue in a mass ratio of 3:1 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to 6.5, and the water content to 60% to obtain the fermentation raw material; Step 2: Add laccase to the fermentation raw material and inoculate with a composite microbial agent. Carry out aerobic fermentation at 25°C, control the aeration rate of each kilogram of fermentation raw material to be 0.4 L / min, and continuously ferment for 1 day to obtain the primary matured material. Among them, the addition amount of laccase is 0.5% of the fermentation raw material, and the inoculation amount of the composite microbial agent is 3% of the fermentation raw material; the composite microbial agent is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 8:1:2; Step 3: Add the primary matured material to a sealed fermentation tank, add a composite enzyme agent and inoculate with Clostridium butyricum, and carry out anaerobic fermentation. Control the fermentation temperature to 25°C and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the composite enzyme agent is 0.5% of the primary matured material, the composite enzyme agent is composed of xylanase and phytase with a mass ratio of 20:3, and the inoculation amount of Clostridium butyricum is 3% of the primary matured material; Step 4: Mix the final matured material and rice hulls, add a composite stabilizing solution, stir at a rotation speed of 100 rpm for 20 min. After stirring evenly, dry at a low temperature of 40°C for 12 h and granulate to obtain the composite microbial enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice hulls, and the composite stabilizing solution is 10:15:20, and the composite stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid, and deionized water with a mass ratio of 1:1:2:80.

[0030] Comparative Example 6 A preparation method of a composite microbial enzyme synergistic fertilizer composition, comprising the following preparation steps: Step 1: Mix cow dung and mushroom residue in a mass ratio of 3:1 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH to 6.5, and the water content to 60% to obtain the fermentation raw material; Step 2: Add laccase to the fermentation raw material and inoculate with a composite microbial agent. Carry out aerobic fermentation at 25°C, control the aeration rate of each kilogram of fermentation raw material to be 0.4 L / min, and continuously ferment for 1 day to obtain the primary matured material. Among them, the addition amount of laccase is 0.5% of the fermentation raw material, and the inoculation amount of the composite microbial agent is 3% of the fermentation raw material; the composite microbial agent is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 8:1:2; Step 3: Add the primary matured material into a sealed fermentation tank, add a compound enzyme agent and inoculate Clostridium butyricum, and carry out anaerobic fermentation. Control the fermentation temperature at 25°C and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the compound enzyme agent is 0.5% of the primary matured material. The compound enzyme agent is composed of xylanase and papain with a mass ratio of 20:5, and the inoculation amount of Clostridium butyricum is 3% of the primary matured material; Step 4: Mix the final matured material and rice chaff, add a compound stabilizing solution, stir at a rotation speed of 100 rpm for 20 min. After stirring evenly, carry out low-temperature drying at 40°C for 12 h and granulate to obtain the compound bacteria and enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice chaff and the compound stabilizing solution is 10:15:20. The compound stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0031] Control Example 7 A preparation method of a compound bacteria and enzyme synergistic fertilizer composition includes the following preparation steps: Step 1: Mix cow dung and mushroom residue according to a mass ratio of 3:1 to obtain a mixture. Add corn straw fragments to the mixture to adjust the carbon-nitrogen ratio to 25:1, control the pH at 6.5, and the water content at 60% to obtain the fermentation raw material; Step 2: Inoculate the compound bacteria agent into the fermentation raw material and carry out aerobic fermentation at 25°C. Control the ventilation volume per kilogram of the fermentation raw material at 0.4 L / min and continuously ferment for 1 day to obtain the primary matured material. Among them, the inoculation amount of the compound bacteria agent is 3% of the fermentation raw material; the compound bacteria agent is composed of Bacillus subtilis, Lactobacillus pentosus and Trichoderma harzianum with an effective viable count ratio of 8:1:2; Step 3: Add the primary matured material into a sealed fermentation tank, add a compound enzyme agent and inoculate Clostridium butyricum, and carry out anaerobic fermentation. Control the fermentation temperature at 25°C and ferment for 5 days to obtain the final matured material. Among them, the addition amount of the compound enzyme agent is 1% of the primary matured material. The compound enzyme agent is composed of laccase, xylanase, papain and phytase with a mass ratio of 28:20:5:3, and the inoculation amount of Clostridium butyricum is 3% of the primary matured material; Step 4: Mix the final matured material and rice chaff, add a compound stabilizing solution, stir at a rotation speed of 100 rpm for 20 min. After stirring evenly, carry out low-temperature drying at 40°C for 12 h and granulate to obtain the compound bacteria and enzyme synergistic fertilizer composition. Among them, the mass ratio of the final matured material, rice chaff and the compound stabilizing solution is 10:15:20. The compound stabilizing solution is composed of sodium lignosulfonate, pullulan, polyglutamic acid and deionized water with a mass ratio of 1:1:2:80.

[0032] Performance Test The comprehensive properties of the compound enzyme synergistic fertilizer compositions (hereinafter referred to as enzyme compound fertilizers) prepared in Examples 1-3 and Comparative Examples 1-7 of the present application were detected respectively, as follows: 1. Detection of basic indexes of fertilizers: The determination was carried out with reference to the industry standard NY / T 525-2021 "Organic Fertilizer" of the Ministry of Agriculture and Rural Affairs, and the test results are shown in Table 1; Table 1 Comprehensive properties of the enzyme compound fertilizers prepared in Examples 1-3 and Comparative Examples 1-7 Test performance Organic matter content (%) Total nutrients (%) Seed germination rate (%) pH Odor level Example 1 56.76 9.84 96.4 5.83 Level 1 Example 2 53.36 9.43 94.5 6.28 Level 1 Example 3 51.54 8.94 95.1 6.54 Level 1 Control Example 1 41.46 5.37 80.4 6.35 Level 3 Control Example 2 48.35 6.32 88.5 7.46 Level 3 Control Example 3 47.29 6.13 87.2 6.34 Level 2 Control Example 4 45.89 5.98 85.4 7.58 Level 3 Control Example 5 40.63 5.22 82.1 6.74 Level 2 Control Example 6 38.68 4.63 73.7 6.53 Level 2 Control Example 7 43.75 5.75 84.8 6.62 Level 3 2. Yield increase test Test plot: Yaqian Village, Laishan District, Yantai City, Shandong Province. The test plot was set up into 12 test points, and the area of each test point was 10 mu, which were respectively recorded as Example Group 1-3, Comparative Example Group 1-7, Test Control Group and Blank Control Group; Test variety: Sunflower SH361; Test time: April 15, 2023 - September 25, 2023; Test design: The enzyme compound fertilizers prepared in Examples 1-3 were respectively applied as base fertilizers in Example Groups 1-3 at 30 kg / mu; the enzyme compound fertilizers prepared in Comparative Examples 1-7 were respectively applied as base fertilizers in Comparative Example Groups 1-7 at 30 kg / mu, the test control group was applied with conventional base fertilizers (conventional base fertilizers: 30 kg / mu of monoammonium phosphate), and the blank control group was not applied with base fertilizers. The topdressing for each test point was: 15 kg / mu of monoammonium phosphate, 12 kg / mu of urea, and 5 kg / mu of potassium chloride. The management of each test point reached "five unifications", that is, unified sunflower variety, unified sowing time, unified fertilization amount, unified sowing density, and unified field management; Test method: On April 15, the test plot was deeply fertilized and covered with soil in the sowing row, and the base fertilizer was covered with plastic film. On April 20, water was applied. On May 1, sunflower seeds SH361 were dibbled beside the fertilization ditch, with a large row spacing of 90 cm, a small row spacing of 40 cm, a plant spacing of 60 cm, and 1700 plants per mu. Topdressing was carried out once on June 28, and on September 25, 10 plants were randomly selected to measure the single-plant sample performance and yield per mu; The test results are shown in Table 2.

[0033] Table 2 Traits and yield per mu of sunflowers treated with different fertilizers Test performance Plant height (cm) Stem diameter (cm) Disk diameter (cm) Empty shell rate (%) Single disk weight (kg) Yield per mu (kg) Example Group 1 217.4 4.13 25.36 13.62 1.256 256.32 Example Group 2 215.7 4.15 25.67 14.28 1.198 248.53 Example Group 3 220.5 4.29 25.94 12.47 1.389 267.75 Control Group 1 203.5 3.47 22.25 18.35 1.039 228.17 Control Group 2 208.5 3.75 23.16 17.79 1.093 232.36 Control Group 3 210.3 3.88 23.65 16.82 1.177 238.10 Control Group 4 208.1 3.69 22.94 17.73 1.114 230.86 Control Group 5 204.8 3.52 22.71 18.13 1.057 229.34 Control Group 6 198.7 3.26 21.93 19.36 1.043 217.89 Control Group 7 207.4 3.58 22.68 17.67 1.103 229.24 Test control group 195.5 3.11 21.35 17.12 0.993 215.64 Blank control group 189.3 2.67 19.37 21.25 0.678 179.43 As can be seen from Table 1 and Table 2, the compound enzyme synergistic fertilizer composition prepared in the present application is not only significantly better than the industry standard of organic fertilizers of the Ministry of Agriculture and Rural Affairs, but also in the application of sunflower planting, this enzyme compound fertilizer can significantly improve the key growth indexes of sunflowers such as plant height, stem diameter, and disk diameter, effectively reduce the empty shell rate, while greatly increasing the single-disk weight and yield per mu, and significantly enhancing the fertilizer utilization rate, bringing significant yield increase and efficiency improvement results for sunflower planting.

[0034] This specific embodiment is only an interpretation of the present application and does not limit the present application. After reading this specification, those skilled in the art can make modifications to this embodiment without creative contributions as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A preparation method of a composite enzyme synergistic fertilizer composition, characterized in that, It includes the following preparation steps: Step 1: Mix livestock and poultry manure and mushroom residue to obtain a mixture. Add crushed corn straw to the mixture to adjust the carbon-nitrogen ratio to 25-30:1, control the pH to 6.5-8.0, and the water content to 60-70% to obtain a fermentation raw material. Step 2: Add laccase to the fermentation raw material and inoculate with a composite microbial agent. Carry out aerobic fermentation at 25-37 °C, control the aeration rate per kilogram of fermentation raw material to 0.4-0.6 L / min, and continuously ferment for 1-2 days to obtain a primary matured material. Step 3: Add the primary matured material to a sealed fermentation tank, add a composite enzyme agent and inoculate with Clostridium butyricum, carry out anaerobic fermentation, control the fermentation temperature to 25-32 °C, and ferment for 5-7 days to obtain a final matured material. Step 4: Mix the final matured material and an adsorption carrier, add a composite stabilizing liquid, stir evenly, then dry at low temperature and granulate to obtain a composite microbial enzyme synergistic fertilizer composition.

2. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that In the said Step 1, the mass ratio of livestock and poultry manure to mushroom residue is 3-5:1-2.

3. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that, In the said Step 2, the addition amount of laccase is 0.5-1% of the fermentation raw material.

4. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that, In the said Step 2, the inoculation amount of the composite microbial agent is 3-8% of the fermentation raw material; the composite microbial agent is composed of Bacillus subtilis, Lactobacillus pentosus, and Trichoderma harzianum with an effective viable count ratio of 8-12:1-3:2-4.

5. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that In the said Step 3, the addition amount of the composite enzyme agent is 0.5-2% of the primary matured material; the composite enzyme agent is composed of xylanase, papain, and phytase with a mass ratio of 20-30:5-12:3-8.

6. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that In the said Step 3, the inoculation amount of Clostridium butyricum is 3-5% of the primary matured material.

7. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that In the said Step 4, the mass ratio of the final matured material, the adsorption carrier, and the composite stabilizing liquid is 10-12:15-35:20-35.

8. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that In the said Step 4, the adsorption carrier is one or more of zeolite powder, rice hulls, diatomaceous earth, and vermiculite.

9. The preparation method of the compound enzyme synergistic fertilizer composition according to claim 1, characterized in that, In the said Step 4, the composite stabilizing liquid is composed of sodium lignosulfonate, pullulan polysaccharide, polyglutamic acid, and deionized water with a mass ratio of 1-3:1-2:2-5:80-120.

10. A composite microbial enzyme synergistic fertilizer composition prepared by the preparation method as described in claims 1-9.