一种检测金黄色葡萄球菌和大肠杆菌的微流控芯片系统及检测方法
By integrating magnetic separation enrichment and SERS detection on a microfluidic chip, and utilizing the composite structure formed by Fe3O4-Van MNPs with Ag@Au-DTNB-Apt NPs and Ag@Au-MBA-Apt NPs, rapid and sensitive detection of Staphylococcus aureus and Escherichia coli in blood was achieved, solving the problem of high detection limit in existing technologies and making it suitable for clinical applications of complex samples.
Patent Information
- Application Number
- CN202510563623.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-30
- Publication Date
- 2026-07-17
- Estimated Expiration
- 2045-04-30
AI Technical Summary
Existing technologies make it difficult to achieve rapid and sensitive detection of Staphylococcus aureus and Escherichia coli in blood samples on microfluidic chips, especially due to high detection limits in complex backgrounds and limited applicability.
A microfluidic chip system integrating magnetic separation enrichment and SERS detection was constructed. Bacteria were captured by Fe3O4-Van MNPs and combined with Ag@Au-DTNB-Apt NPs and Ag@Au-MBA-Apt NPs to form a composite structure, and in situ SERS detection was performed to achieve specific identification and quantitative analysis of Staphylococcus aureus and Escherichia coli.
It enables rapid and sensitive detection of Staphylococcus aureus and Escherichia coli in blood, with detection limits as low as 2.01 CFU/mL and 3.28 CFU/mL, respectively. This significantly improves detection sensitivity and lowers the detection limit, making it suitable for clinical applications with complex samples.
Smart Images
Figure CN120427898B_ABST