A field-based large-scale culture device suitable for MICP microbial fermentation

Abstract

This invention discloses a large-scale on-site culture device suitable for MICP microbial fermentation, relating to the field of microbial culture technology. It includes a fermentation tank with oxygen pumps uniformly arranged at the bottom and twin-screw pumps symmetrically arranged in the middle. An intelligent control box is installed on the twin-screw pumps. An exhaust pipe is located on the upper surface of the fermentation tank, and a two-way valve is installed on the outer surface of the middle section. This design employs dynamic pumping of the bacterial solution, allowing the twin-screw pumps to pump the solution from different locations at the bottom of the fermentation tank. This avoids the uneven distribution of bacterial activity at the bottom, which is unavoidable with traditional stirring devices. The twin-screw pumps uniformly and dynamically pump the bacterial solution from the bottom of the fermentation tank to the top, creating a vertically circulating mixing mode. This mixing mode breaks down the concentration gradient in the bacterial solution, ensuring a uniform distribution of nutrients and oxygen, and improving the growth rate and metabolic activity of the bacteria.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, specifically to a large-scale on-site culture device suitable for MICP microbial fermentation. Background Technology

[0002] Microbial-induced calcium carbonate precipitation (MICP) technology is widely used in soil reinforcement, concrete repair, and other fields. Current technologies primarily rely on laboratory-based microbial culture equipment, characterized by: fixed laboratory equipment such as fermenters and shakers requiring stable power and temperature control; small-scale cultivation methods with large, complex equipment that is difficult to apply directly to engineering sites; reliance on manual operation requiring frequent monitoring of pH, temperature, and bacterial activity, making it unsuitable for continuous on-site operations; and outdoor microbial culture often employs traditional fermenters for manual cultivation, using top agitators or air pumps for aeration, resulting in sedimentation at the bottom, low mixing efficiency, and limited control over bacterial activity.

[0003] In summary, existing microbial culture equipment has the following disadvantages:

[0004] (1) Unable to be applied on a large scale in the field: Laboratory equipment is large in size, consumes a lot of energy, and relies on fixed infrastructure (such as constant temperature air conditioning), making it difficult to deploy in the field or in environments without power.

[0005] (2) Uneven mixing of bacterial solution: Traditional stirring devices cannot avoid sedimentation, resulting in uneven distribution of bacterial activity at the bottom and unstable quality during large-scale cultivation (structural defect: the stirrer is located at the top of the container, making it difficult to stir the sediment at the bottom).

[0006] (3) Inaccurate temperature control: The external heating method has a slow response and is difficult to control the temperature evenly (the principle is flawed: it relies on an external heat source and does not come into direct contact with the bacterial solution).

[0007] (4) Lack of monitoring of bacterial activity: The lack of real-time monitoring methods such as conductivity means that manual detection of parameters such as pH and activity cannot be fed back in real time, which affects the quality of culture.

[0008] Therefore, a field large-scale culture device suitable for MICP microbial fermentation is proposed to solve the above problems. Summary of the Invention

[0009] In view of this, the technical problem to be solved by the present invention is to provide a field large-scale culture device suitable for MICP microbial fermentation, so as to solve the problems that have occurred in the background art.

[0010] To achieve the above objectives, the present invention provides the following technical solution: a field large-scale culture device suitable for MICP microbial fermentation, comprising a fermentation tank, wherein an oxygen pump is uniformly arranged at the bottom of the fermentation tank, a twin-screw pump is symmetrically arranged in the middle of the fermentation tank, an intelligent control box is installed on the twin-screw pump, an exhaust pipe is opened on the upper surface of the fermentation tank, and a two-way valve is installed on the outer surface of the middle part of the fermentation tank; the field large-scale culture device suitable for MICP microbial fermentation further includes a multi-point monitoring aeration mechanism and a reflux adjustment mechanism;

[0011] The multi-point monitoring aeration mechanism is located at the bottom of the fermentation tank and is used to agitate and aerate the culture medium at the bottom of the fermentation tank.

[0012] The reflux adjustment mechanism is located above the multi-point monitoring aeration mechanism, and is used for dynamic monitoring of the culture medium and prevention of sedimentation.

[0013] Preferably, the multi-point monitoring aeration mechanism includes a sedimentation scraper, the middle of which is rotatably connected to the bottom of the fermentation tank, the oxygen pump is evenly installed on the sedimentation scraper, a drive shaft is fixedly connected to the middle of the sedimentation scraper, a first drive tooth is fixedly connected to the outer surface of the drive shaft, and a second drive tooth meshes with the tooth surface of the first drive tooth.

[0014] Preferably, the bottom of the second transmission tooth is provided with an inner tooth disk, and a meshing groove is provided in the middle of the inner tooth disk. The second transmission tooth is uniformly rotatably connected to the middle of the inner tooth disk. The tooth surface of the second transmission tooth away from the first transmission tooth meshes in the meshing groove provided in the inner tooth disk. Arc-shaped pusher plates are uniformly installed on the outer surface of the inner tooth disk.

[0015] Preferably, the arc-shaped push plate is provided with uniformly spaced guide holes, the upper end of the drive shaft is fixedly connected to a connecting plate, the inner wall of the fermentation tank is rotatably connected to a rotating ring, the two ends of the connecting plate are fixedly connected to the rotating ring, and the twin-screw pump is symmetrically mounted on the connecting plate.

[0016] Preferably, the reflux adjustment mechanism includes a filter screen cover, which is installed at the bottom of a twin-screw pump. A servo drive motor is installed on the twin-screw pump, and a drive tooth is fixedly connected to the drive shaft of the servo drive motor. An inner toothed ring is rotatably connected to the inner surface of the filter screen cover, and a sliding tooth groove is opened on the outer surface of the inner toothed ring. The tooth surface of the drive tooth meshes in the sliding tooth groove of the inner toothed ring.

[0017] Preferably, the inner tooth surface of the inner tooth ring is engaged with a third transmission tooth, the third transmission tooth is circumferentially connected to the filter screen cover, the filter screen cover is provided with a guide groove on the circumference, the filter screen cover is provided with an L-shaped multi-functional plate on the circumference, the L-shaped multi-functional plate is uniformly provided with sliding teeth, and the sliding tooth surfaces of the L-shaped multi-functional plate are engaged with the third transmission tooth.

[0018] Preferably, a fixing block is fixedly connected to the side of the L-shaped multifunctional plate near the sliding teeth, and the fixing block is slidably connected in the guide groove. A cleaning scraper is fixedly connected to the end of the L-shaped multifunctional plate away from the sliding teeth, and the cleaning scraper slides on the upper surface of the filter holes at the bottom of the filter screen.

[0019] Preferably, the twin-screw pump body is equipped with a resistance wire and a temperature sensor to achieve rapid preheating of the bacterial solution.

[0020] Compared with the prior art, the present invention provides a field large-scale culture device suitable for MICP microbial fermentation, which has the following beneficial effects:

[0021] 1. Large-scale on-site cultivation: The modular design supports the parallel connection of multiple devices. The cultivation capacity of a single fermentation tank can reach 500L, and can be expanded to thousands of liters after parallel connection to meet the needs of engineering sites.

[0022] 2. This solution abandons the traditional top-mounted stirring device, which easily causes sedimentation at the bottom of the bacterial solution. The use of a twin-screw pump to dynamically rotate in the bacterial tank can prevent sedimentation of the bacterial solution.

[0023] 3. This solution uses dynamic aeration at the bottom of the fermentation tank, which, compared to traditional fixed aeration, makes the aerobic respiration of the bacterial solution more uniform and reduces the mortality of colonies due to anaerobic conditions.

[0024] 4. Dynamically collect pH-related data of bacterial solutions to reduce errors from sampling in a single area.

[0025] 5. The bacterial solution is extracted and the temperature is controlled by heating the inner cavity of the twin-screw pump through the resistance wire on the inner wall of the cylinder and the hot water heat exchange.

[0026] 6. Intelligent and environmentally friendly:

[0027] (1) Real-time monitoring data is transmitted wirelessly to mobile terminals to achieve remote monitoring.

[0028] (2) A silent fan combined with an activated carbon filter layer prevents odors from spreading.

[0029] (3) The conductivity needle is linked with the pH sensor to reflect the metabolic state of microorganisms in real time.

[0030] (4) Based on multi-parameter feedback, automated control reduces manual intervention and improves cultivation efficiency. Attached Figure Description

[0031] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0032] Figure 2 This is a schematic diagram of a three-dimensional structure of the present invention in half section.

[0033] Figure 3 For the present invention Figure 2 Enlarged view of point A in the middle;

[0034] Figure 4 This is a schematic diagram of the structural connection relationship of the reflux adjustment mechanism of the present invention;

[0035] Figure 5 For the present invention Figure 4 Enlarged view at point B in the middle;

[0036] Figure 6 This is an auxiliary schematic diagram showing the structural connection relationship of the reflux adjustment mechanism of the present invention;

[0037] Figure 7 This is a diagram showing the connection relationships of the L-shaped multifunctional plate structure of the present invention.

[0038] Figure 8 This is a flowchart of the automatic temperature control process of the present invention.

[0039] In the picture:

[0040] 1. Fermentation tank; 11. Oxygen pump; 12. Twin screw pump; 13. Intelligent control box; 14. Exhaust pipe; 15. Two-way valve;

[0041] 2. Multi-point monitoring aeration mechanism; 21. Sedimentation scraper; 22. Drive shaft; 23. First drive gear; 24. Second drive gear; 25. Internal gear disc; 26. Arc-shaped pusher plate; 27. Connecting plate; 28. Rotary ring;

[0042] 3. Reflux adjustment mechanism; 31. Filter screen cover; 32. Drive gear; 33. Inner gear ring; 34. Third transmission gear; 35. L-shaped multi-functional plate; 36. Scraper; 37. Guide groove; 38. Fixing block. Detailed Implementation

[0043] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0045] For an example, please refer to... Figures 1 to 8 As shown:

[0046] To address the problems mentioned in the technical solutions, this application provides a field large-scale culture device suitable for MICP microbial fermentation, including a fermentation tank 1, an oxygen pump 11 uniformly arranged at the bottom of the fermentation tank 1, a twin-screw pump 12 symmetrically arranged in the middle of the fermentation tank 1, an intelligent control box 13 installed on the twin-screw pump 12, an exhaust pipe 14 opened on the upper surface of the fermentation tank 1, a two-way valve 15 installed on the outer surface of the middle of the fermentation tank 1, and also includes a multi-point monitoring aeration mechanism 2 and a reflux adjustment mechanism 3;

[0047] The multi-point monitoring aeration mechanism 2 is set at the bottom of the fermentation tank 1. The multi-point monitoring aeration mechanism 2 is used to stir and aerate the culture liquid at the bottom of the fermentation tank 1.

[0048] The reflux regulating mechanism 3 is located above the multi-point monitoring aeration mechanism 2. The reflux regulating mechanism 3 is used for dynamic monitoring of the culture medium and to prevent sedimentation.

[0049] Among them, the inner wall of the fermentation tank 1 is embedded with a resistance wire, and the intelligent control box 13 is equipped with a pH sensor, a temperature sensor, an adaptive adjustment algorithm of the control logic unit, and a conductivity sensor. The conductivity sensor is used to quantitatively assess the activity of microorganisms. The twin screw pump 12 is equipped with a resistance wire and a temperature sensor inside the pump body to achieve rapid preheating of the bacterial solution.

[0050] like Figure 4 As shown, this design features a heat exchange chamber on the inner wall of the 12, equipped with a temperature sensor and a resistance wire. The temperature sensor controls the heating of the resistance wire. A threaded, rotatable sealing cap is located on the top of the 12 near the drive motor, allowing hot water to be added to the heat exchange chamber. The heating of the hot water by the resistance wire enables heat exchange between the heated water and the bacterial solution via the inner wall of the twin-screw pump 12. This allows for more precise temperature control of the bacterial solution. The temperature of the culture medium can be continuously controlled by the twin-screw pump 12 and the temperature control device inside the fermentation tank 1. The bacterial solution can be kept at a constant temperature of ±1℃ in an environment ranging from -10℃ to 40℃, adapting to extreme outdoor temperatures.

[0051] The specific temperature control process is as follows: Figure 8 As shown.

[0052] In this design, pulleys are evenly installed on the lower surface of fermentation tank 1, allowing for rapid movement of fermentation tank 1. Two-way valves 15 are installed on both sides of fermentation tank 1. When facing the need for large-scale mixed culture of bacterial solutions, only fermentation tank 1 needs to be added. Through the quick connection of the two-way valves 15, the modular design supports parallel connection of multiple fermentation tanks 1. A single fermentation tank 1 can have a culture capacity of up to 500L, which can be expanded to several thousand liters when connected in parallel. It is detachable and combinable, adaptable to complex field conditions, and meets the needs of engineering sites. Simultaneously, the temperature of the culture medium can be constantly controlled by the twin-screw pump 12 and the internal temperature control device of fermentation tank 1, maintaining a constant temperature of ±1℃ for the bacterial solution in an environment ranging from -10℃ to 40℃, adapting to extreme outdoor temperatures.

[0053] Specifically, such as Figure 3 As shown, the sedimentation scraper 21 is rotatably connected to the bottom of the fermentation tank 1 in the middle. The oxygen pump 11 is evenly installed on the sedimentation scraper 21. A drive shaft 22 is fixedly connected to the middle of the sedimentation scraper 21. A first drive tooth 23 is fixedly connected to the outer surface of the drive shaft 22. The tooth surface of the first drive tooth 23 meshes with a second drive tooth 24. An inner toothed disc 25 is provided at the bottom of the second drive tooth 24. A meshing groove is opened in the middle of the inner toothed disc 25. The second drive tooth 24 is evenly rotatably connected to the middle of the inner toothed disc 25. The tooth surface of the second drive tooth 24 away from the first drive tooth 23 meshes in the meshing groove opened in the inner toothed disc 25. An arc-shaped pusher plate 26 is evenly installed on the outer surface of the inner toothed disc 25. Guide holes are evenly opened on the arc-shaped pusher plate 26.

[0054] A drive motor is installed at the bottom of the fermentation tank 1. The drive motor drives the middle part of the sedimentation scraper 21 to rotate. The rotation of the sedimentation scraper 21 drives the first transmission gear 23 to drive the second transmission gear 24 to rotate in the opposite direction. The rotation of the second transmission gear 24 drives the inner toothed disk 25 to rotate synchronously. This solution can clean the sediment at the bottom of the fermentation tank 1 through the scraper at the bottom of the sedimentation scraper 21, preventing uniform sediment from adhering to the bottom of the fermentation tank 1. Through the rotation of the sedimentation scraper 21 and the inner toothed disk 25 rotating in the opposite direction to the sedimentation scraper 21, the bottom of the fermentation tank 1 can be stirred by the arc-shaped push plate 26 uniformly welded on the inner toothed disk 25. The flow guide hole on the arc-shaped push plate 26 can accelerate the flow of bacterial liquid at the bottom of the fermentation tank 1, further preventing the bacterial liquid from settling.

[0055] Furthermore, by evenly installing an oxygen pump 11 on the sedimentation scraper 21, aeration is achieved at the bottom of the fermentation tank 1. Compared to traditional technology where aeration is fixed at the bottom of the fermentation tank 1, resulting in localized aeration of the culture medium, this solution uses an oxygen pump 11 that rotates synchronously with the sedimentation scraper 21. While the sedimentation scraper 21 cleans and stirs the bottom of the fermentation tank 1, the oxygen pump 11 simultaneously aerates the bottom of the fermentation tank 1. The rotation of the oxygen pump 11 ensures uniform aeration coverage at the bottom of the fermentation tank 1, thereby reducing localized aeration and allowing the bacterial solution to undergo a more uniform aerobic reaction, increasing the uniformity of the culture.

[0056] Furthermore, a connecting plate 27 is fixedly connected to the upper end of the drive shaft 22, and a rotating ring 28 is rotatably connected to the inner wall of the fermentation tank 1. Both ends of the connecting plate 27 are fixedly connected to the rotating ring 28, and the twin screw pump 12 is symmetrically installed on the connecting plate 27.

[0057] This design uses a drive shaft 22 to synchronously drive the rotation of the internal gear disc 25 and the connecting plate 27, causing the connecting plate 27 to rotate in the opposite direction to the internal gear disc 25. The connecting plate 27 drives the twin-screw pump 12 to rotate evenly in the fermentation tank 1. Compared to a fixed twin-screw pump 12 installation, this design uses dynamic pumping of the bacterial solution, allowing the twin-screw pump 12 to pump the bacterial solution from different positions at the bottom of the fermentation tank 1. This avoids the sedimentation that traditional stirring devices cannot prevent, which leads to uneven distribution of bacterial activity at the bottom. By using the twin-screw pump 12 to uniformly and dynamically pump the bacterial solution from the bottom of the fermentation tank 1 to the top, the twin-screw pump 12 draws the bacterial solution from the bottom of the culture vessel to the top, forming an up-and-down circulating mixing mode. This mixing mode can break the concentration gradient in the bacterial solution, making nutrients and oxygen evenly distributed, and improving the growth rate and metabolic activity of the bacteria.

[0058] Specifically, such as Figure 6 As shown, a servo drive motor is installed on the twin screw pump 12, and a drive tooth 32 is fixedly connected to the drive shaft of the servo drive motor. An inner tooth ring 33 is rotatably connected to the inner surface of the filter screen 31. A sliding tooth groove is opened on the outer surface of the inner tooth ring 33, and the tooth surface of the drive tooth 32 meshes in the sliding tooth groove of the inner tooth ring 33.

[0059] The rotation of the servo motor can drive the drive gear 32 to drive the internal gear ring 33 to rotate and adjust within the inner wall of the filter screen 31.

[0060] The inner toothed ring 33 has a third transmission tooth 34 meshing on its inner tooth surface. The third transmission tooth 34 is circumferentially connected to the filter screen cover 31. The filter screen cover 31 has a guide groove 37 on its circumference. An L-shaped multi-functional plate 35 is arranged on the circumference of the filter screen cover 31. Sliding teeth are evenly arranged on the L-shaped multi-functional plate 35. The tooth surfaces of the sliding teeth of the L-shaped multi-functional plate 35 mesh with the third transmission tooth 34. A fixing block 38 is fixedly connected to the side of the L-shaped multi-functional plate 35 near the sliding teeth. The fixing block 38 is slidably connected in the guide groove 37. A cleaning scraper 36 is fixedly connected to the end of the L-shaped multi-functional plate 35 away from the sliding teeth. The cleaning scraper 36 slides on the upper surface of the filter holes at the bottom of the filter screen cover 31.

[0061] Among them, such as Figure 6 As shown, the L-shaped multifunctional plates 35 are arranged in an L-shape. The sliding between the L-shaped multifunctional plates 35 allows control of the size of the inlet of the twin-screw pump 12. Simultaneously, by controlling the inlet size of the L-shaped multifunctional plates 35, the sliding of the scraper 36 on the surface of the filter screen 31 prevents blockage caused by bacterial solution adhesion. This design allows for precise adjustment of the bacterial solution delivery rate by controlling the size of the inlet of the twin-screw pump, thus adapting to the needs of different culture stages. In the early stages of culture, the inlet size can be reduced to avoid excessive shear force on the bacteria; in the later stages of culture, the inlet size can be increased to accelerate the mixing speed. Mixing via the twin-screw pump 12 prevents bacterial deposition at the bottom of the culture vessel, reducing bacterial death and degradation.

[0062] The specific implementation steps in the above embodiments are as follows:

[0063] Step 1: On-site assembly and startup of fermentation tank 1; transport the fermentation tank 1 culture unit to the project site, connect the pipeline, power supply and control module through the quick interface of the two-way valve 15; inject preheated hot water into the twin screw pump 12, the initial temperature is set according to the environment, and start the internal circulation.

[0064] The specific steps are as follows:

[0065] First, start the drive motor installed at the bottom of fermentation tank 1. Starting the drive motor will drive the following... Figure 3The sedimentation scraper 21 shown starts to rotate synchronously. The rotation of the sedimentation scraper 21 cleans the bottom of the fermentation tank 1, preventing bacterial liquid from settling and adhering to the bottom. Simultaneously, driven by the drive shaft 22, the inner gear disc 25 drives the arc-shaped pusher plate 26 to rotate in the opposite direction to the sedimentation scraper 21 through the meshing of the second drive gear 24. The rotation of the arc-shaped pusher plate 26 accelerates the stirring of the bacterial liquid at the bottom of the fermentation tank 1. The evenly spaced guide holes on the arc-shaped pusher plate 26 also accelerate the flow of the bacterial liquid at the bottom of the fermentation tank 1. Simultaneously, the drive shaft 22 synchronously drives the connecting plate 27 to drive the twin-screw pump 12 to rotate within the fermentation tank 1. The twin-screw pump 12 rotates uniformly within the fermentation tank 1. Compared to a fixed twin-screw pump 12 installation, this design uses dynamic pumping to draw out the bacterial solution, allowing the twin-screw pump 12 to draw out the bacterial solution at different locations at the bottom of the fermentation tank 1. This avoids the sedimentation that traditional stirring devices cannot prevent, which leads to uneven distribution of bacterial activity at the bottom. By uniformly and dynamically drawing the bacterial solution from the bottom of the fermentation tank 1 to the top of the fermentation tank 1, the twin-screw pump 12 draws the bacterial solution from the bottom of the culture vessel to the upper part, forming an up-and-down circulating mixing mode. This mixing mode can break the concentration gradient in the bacterial solution, making nutrients and oxygen evenly distributed, and improving the growth rate and metabolic activity of the bacteria.

[0066] Step 2: Loading and spreading of bacterial solution.

[0067] Inject bacterial solution and culture medium into the first fermentation tank 1. Connect the new fermentation tank 1 to the new fermentation tank 1 step-by-step via the two-way valve 15. The system automatically balances the flow rate of each unit. Pour culture medium into the new fermentation tank 1. Open the two-way valve 15 between the first fermentation tank 1 and the new fermentation tank 1 to transfer the cultured bacteria from the first fermentation tank 1 to the new fermentation tank 1. Circulate this process until fermentation tank 1 is full of bacteria. At this point, bacteria with different activities can be obtained, and bacteria with the same activity can be obtained at different time periods.

[0068] Step 3: Example of intelligent control.

[0069] The resistance wire embedded in the inner wall of fermentation tank 1 is activated to heat the ambient temperature. Once the specified temperature is reached, the control unit increases the power of the resistance wire and simultaneously accelerates the flow rate of the twin screw pump 12 to balance the heat.

[0070] The specific operation process of controlling the size of the liquid inlet at the bottom of the twin-screw pump 12 is as follows:

[0071] First, the servo motor installed on the twin-screw pump 12 is started by the control system in the intelligent control box 13. At this time, the rotation of the servo motor drives the drive gear 32 to drive the inner gear ring 33 to rotate. When the inner gear ring 33 rotates, the inner tooth surface of the inner gear ring 33 meshes with the L-shaped multifunctional plate 35. The rotation of the inner gear ring 33 drives the third transmission gear 34 to drive the L-shaped multifunctional plate 35 to slide on the filter screen cover 31. By controlling the sliding between the L-shaped multifunctional plates 35, the size of the liquid inlet between the L-shaped multifunctional plate 35 and the twin-screw pump 12 can be controlled. This solution can precisely adjust the delivery volume of bacterial solution by controlling the size of the liquid inlet of the twin-screw pump, thereby adapting to the needs of different culture stages. In the early stage of culture, the size of the liquid inlet can be reduced to avoid excessive shearing force on the bacteria. In the later stage of culture, the size of the liquid inlet can be increased to accelerate the mixing speed. Mixing by the twin-screw pump 12 can prevent bacteria from depositing at the bottom of the culture vessel, reducing bacterial death and degradation.

[0072] Furthermore, buffer solution is automatically injected when the pH exceeds the limit; the oxygen meter is activated at the bottom of the fermentation tank at regular intervals to enhance bacterial activity. When the conductivity needle detects a decrease in activity, the oxygen pump 11 is automatically triggered to increase pressure and aerate. Since the oxygen pump 11 is installed on the sedimentation scraper 21, the rotation of the sedimentation scraper 21 can synchronously drive the oxygen pump 11 to start rotating. This scheme uses the oxygen pump 11 to rotate synchronously with the sedimentation scraper 21. When the sedimentation scraper 21 cleans and stirs the bottom of the fermentation tank 1, the oxygen pump 11 can simultaneously aerate the bottom of the fermentation tank 1. The rotation of the oxygen pump 11 can achieve uniform aeration coverage of the bottom of the fermentation tank 1, thereby reducing the occurrence of local aeration phenomena, allowing the bacterial solution to carry out aerobic reactions more evenly, and increasing the uniformity of the culture.

[0073] like Figure 8 As shown, when the sensor installed in the heat exchange chamber 12 detects that the bacterial solution has not reached a suitable temperature, the system starts reading the real-time temperature of the sensor under the automatic start-up control. At this time, the control circuit in the system compares the received electrical signal with the preset temperature value, which is set according to the needs of bacterial culture and is usually a constant value. When the control circuit detects that the actual temperature of the bacterial solution is lower than the preset temperature, it sends a control signal. This control signal activates the resistance wire to heat the solution. The heat generated by the resistance wire is transferred to the water in the heat exchange chamber. Through heat exchange between the water and the inner wall of 12, the inner wall of 12 can control the temperature of the bacterial solution, thereby gradually increasing the temperature of the bacterial solution.

[0074] During the heating process, the temperature sensor continuously monitors the temperature changes of the bacterial solution. When the temperature of the bacterial solution approaches the preset temperature, the temperature sensor feeds this information back to the control circuit. The control circuit then adjusts the heating power of the resistance wire based on the feedback signal to ensure that the temperature of the bacterial solution remains stable near the preset value. Through continuous monitoring by the temperature sensor and adjustment by the control circuit, the temperature of the bacterial solution eventually stabilizes near the preset value, forming a stable temperature-controlled environment.

[0075] Step 4: Exhaust treatment.

[0076] A silent fan is installed in the exhaust pipe 14. The silent fan adjusts its speed according to the gas sensor data. The exhaust port is connected to an extension pipe to an uninhabited area 30 meters away.

[0077] Please refer to the above work process. Figures 1 to 8 .

[0078] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element. Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A field-based large-scale culture device suitable for MICP microbial fermentation, characterized in that, The device includes a fermentation tank (1), an oxygen pump (11) evenly arranged at the bottom of the fermentation tank (1), a twin screw pump (12) symmetrically arranged in the middle of the fermentation tank (1), an intelligent control box (13) installed on the twin screw pump (12), an exhaust pipe (14) opened on the upper surface of the fermentation tank (1), and a two-way valve (15) installed on the outer surface of the middle of the fermentation tank (1). The large-scale on-site cultivation device suitable for MICP microbial fermentation also includes a multi-point monitoring aeration mechanism (2) and a reflux adjustment mechanism (3). The multi-point monitoring aeration mechanism (2) is set at the bottom of the fermentation tank (1). The multi-point monitoring aeration mechanism (2) is used to stir and aerate the culture liquid at the bottom of the fermentation tank (1). The reflux adjustment mechanism (3) is located above the multi-point monitoring aeration mechanism (2), and the reflux adjustment mechanism (3) is used for dynamic detection of the culture medium and prevention of sedimentation. The reflux adjustment mechanism (3) includes a filter screen cover (31), which is installed at the bottom of a twin-screw pump (12). A servo drive motor is installed on the twin-screw pump (12), and a drive tooth (32) is fixedly connected to the drive shaft of the servo drive motor. An inner toothed ring (33) is rotatably connected to the inner surface of the filter screen cover (31). A sliding tooth groove is opened on the outer surface of the inner toothed ring (33), and the tooth surface of the drive tooth (32) meshes in the sliding tooth groove of the inner toothed ring (33). The inner toothed ring (33) has a third transmission tooth (34) meshing on its inner tooth surface. The third transmission tooth (34) is circumferentially connected to the filter screen cover (31). The filter screen cover (31) has a guide groove (37) circumferentially provided. The filter screen cover (31) has an L-shaped multi-functional plate (35) circumferentially provided. The L-shaped multi-functional plate (35) has evenly provided sliding teeth. The sliding tooth surfaces of the L-shaped multi-functional plate (35) mesh with the third transmission tooth (34). The L-shaped multifunctional plate (35) is fixedly connected to a fixing block (38) on the side near the sliding teeth. The fixing block (38) is slidably connected in the guide groove (37). The L-shaped multifunctional plate (35) is fixedly connected to a cleaning scraper (36) at the end away from the sliding teeth. The cleaning scraper (36) slides on the upper surface of the filter hole at the bottom of the filter screen cover (31). The twin-screw pump (12) is equipped with a resistance wire and a temperature sensor inside the pump body to achieve rapid preheating of the bacterial solution.

2. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 1, characterized in that: The multi-point monitoring aeration mechanism (2) includes a sedimentation scraper (21), the middle part of which is rotatably connected to the bottom of the fermentation tank (1), the oxygen pump (11) is evenly installed on the sedimentation scraper (21), the middle part of which is fixedly connected to a drive shaft (22), the outer surface of which is fixedly connected to a first drive tooth (23), and the tooth surface of the first drive tooth (23) is engaged with a second drive tooth (24).

3. The on-site large-scale culture device suitable for MICP microbial fermentation according to claim 2, characterized in that: The bottom of the second transmission tooth (24) is provided with an inner tooth disk (25), and a meshing groove is provided in the middle of the inner tooth disk (25). The second transmission tooth (24) is uniformly rotated and connected to the middle of the inner tooth disk (25). The tooth surface of the second transmission tooth (24) away from the first transmission tooth (23) meshes in the meshing groove provided in the inner tooth disk (25). Arc-shaped pusher plates (26) are uniformly installed on the outer surface of the inner tooth disk (25).

4. The on-site large-scale culture device for MICP microbial fermentation according to claim 3, characterized in that: The arc-shaped push plate (26) is provided with uniformly distributed guide holes. The upper end of the drive shaft (22) is fixedly connected to a connecting plate (27). The inner wall of the fermentation tank (1) is rotatably connected to a rotating ring (28). The two ends of the connecting plate (27) are fixedly connected to the rotating ring (28). The twin screw pump (12) is symmetrically installed on the connecting plate (27).

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