用于获得猪多能干细胞的培养基以及猪多能干细胞的诱导培养方法
By using LACID medium and iCD3 medium combined with mechanical separation, the induction culture process of porcine pluripotent stem cells was optimized, solving the problems of long induction cycle and low efficiency in existing technologies, and achieving efficient acquisition of porcine pluripotent stem cells with pluripotency and developmental potential.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- WESTLAKE UNIV
- Filing Date
- 2025-04-28
- Publication Date
- 2026-07-17
AI Technical Summary
In existing porcine pluripotent stem cell (piPSC) culture processes, the induction period is relatively long, the efficiency of induced cell clone formation in the induction medium is low, and the maintenance of pluripotency of piPSCs needs to be improved.
LACID medium, which is based on Knockout DMEM medium and supplemented with fetal bovine serum, Knockout serum substitute, GlutaMAX, non-essential amino acids, β-mercaptoethanol, human leukemia inhibitory factor, activin A, GSK-3 inhibitor, Wnt signaling pathway inhibitor, and L-ascorbic acid-2-phosphate trisodium salt, was used to induce porcine pluripotent stem cell culture in combination with iCD3 medium and mechanical separation method.
The induction time of porcine pluripotent stem cells was shortened to a minimum of 14 days, improving the induction efficiency. The obtained piPSCs exhibited pluripotency and developmental potential, and were able to differentiate into cardiomyocytes and teratomas. When cultured under feeder-free conditions, they showed rapid proliferation and good developmental ability.
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Figure CN120464577B_ABST