Animal limb measuring device with multi-positioning structure

Abstract

The application discloses an animal limb measuring device with a multi-positioning structure, which is convenient to operate and can improve detection accuracy. The animal limb measuring device with the multi-positioning structure comprises a base, a detection platform arranged at a middle position of the base, a first stand column arranged at one side of the detection platform, a second stand column arranged at the other side of the detection platform, a sliding guide rail arranged on the second stand column, a first sliding block and a second sliding block arranged on the sliding guide rail, a detection support arranged below the first sliding block, a distance detection device detachably connected and mounted on the detection support, a telescopic transverse support arranged at an upper end of the first stand column, a detachable overflow device arranged on the transverse support, and a detachable animal fixing device arranged at a lower end of the second telescopic support. The animal limb measuring device with the multi-positioning structure is convenient to operate, can record and trace a detection process, can improve detection effect, and can reduce damage to a detection animal.

Description

Technical Field

[0001] This invention relates to the field of laboratory animal science, and in particular to an animal limb measurement device with a multi-positioning structure. Background Technology

[0002] Animal limb volume measurement has wide applications in medical experiments, primarily for assessing pathological states, treatment effects, physiological changes, or drug responses. It is frequently used in laboratory zoology.

[0003] Animal limb volume measurement devices used in experiments typically include a detection frame, a fluid replenishment device, and a volume detection device. For example, the UGO BASILE limb swelling detection device from Italy, sold by Guangzhou Kezhilan Instrument Co., Ltd.

[0004] The volume detection device includes two connected measuring cups. One measuring cup is used to place the animal limb to be measured, and the other cup is used to detect volume changes via a gradient pressure sensor.

[0005] In the process of measuring the volume of an animal limb, the experimenter first marks the immersion depth on the limb to be tested. Then, liquid is added to the measuring cup using a replenishment device until the liquid level reaches the marked level. The experimenter then holds the animal in place and slowly lowers the limb into the measuring cup until it reaches the marked depth. Since the two measuring cups are connected, the water levels in both cups change as the limb is immersed. Existing techniques typically detect the voltage between the upper and lower electrodes within the measuring cups to indicate the degree of swelling in the animal limb.

[0006] Because existing technologies for detecting limb swelling in animals require manual positioning of the animal, they are not easy to operate. Furthermore, researchers need to observe the test results and the position of the animal's limb immersed in the liquid while positioning the animal, which can easily lead to operational errors and affect the test results. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to provide an animal limb measurement device with a multi-positioning structure that is easy to operate and can improve detection accuracy.

[0008] The technical solution adopted by the present invention to solve its technical problem is: an animal limb measuring device with a multi-positioning structure, including a base; a detection platform is provided in the middle of the base;

[0009] The detection platform has a first column on one side and a second column on the other side; a sliding guide rail is provided on the second column; a first slider and a second slider are provided on the sliding guide rail;

[0010] A first telescopic bracket is provided below the first slider; a second telescopic bracket is provided for the second slider; a detection bracket is provided at the lower end of the first telescopic bracket; a detachable distance detection device is installed on the detection bracket;

[0011] The upper end of the first column is provided with a retractable horizontal support; the horizontal support is provided with a detachable overflow device; the lower end of the second telescopic support is provided with a detachable animal restraint device.

[0012] One end of the detection bracket is rotatably connected to the lower end of the first telescopic bracket; the distance detection device includes an annular mounting frame; a distance measuring sensor evenly distributed along the circumference is mounted on the annular mounting frame; an adjustment device for adjusting the distance measuring angle of the distance measuring sensor is provided on the annular mounting frame; an annular junction box is provided above the annular mounting frame; a horizontal support frame is provided on one side of the annular junction box; a vertical support frame is provided on the horizontal support frame, and a camera is mounted on the vertical support frame;

[0013] The animal restraint device is used to restrain the animal being tested; the overflow device is used to measure the volume of the part of the animal to be tested using the drainage method.

[0014] Furthermore, the annular mounting bracket is provided with a mounting groove that matches the ranging sensor, and the two sides of the ranging sensor are hinged to the side wall of the mounting groove via a rotating shaft; the rotating shaft on one side of the ranging sensor extends out of the annular mounting bracket and is connected to the driving device for transmission.

[0015] Furthermore, the overflow device adopts a segmented overflow device; the overflow device includes a lower overflow pipe and an upper overflow pipe; the lower end of the lower overflow pipe is provided with a liquid inlet pipe; a valve is provided on the liquid inlet pipe; one end of the upper overflow pipe is inserted into the lower overflow pipe and is threadedly engaged with the lower overflow pipe.

[0016] An overflow pipe is provided on one side of the upper overflow pipe; one end of the overflow pipe is connected to the upper overflow pipe, and an overflow liquid volume detection device is provided below the other end;

[0017] The upper overflow pipe is equipped with pressure sensors that are evenly distributed vertically, and the position of the first pressure sensor at the upper end is lower than the position of the overflow pipe.

[0018] Furthermore, an installation groove is provided on the inner wall of the upper overflow pipe; a detachable installation block is installed in the installation groove; the pressure sensor is installed on the installation block and is evenly distributed vertically.

[0019] Furthermore, the overflow liquid volume detection device adopts a graduated vertical collection cylinder; an exhaust pipe is provided at the upper end of the vertical collection cylinder; and an openable and closable drain pipe is provided at the bottom of the vertical collection cylinder.

[0020] Alternatively, the overflow liquid volume detection device may be a weighing pan; a solenoid valve may be installed on the overflow pipe; an overflow level gauge may be installed in the upper overflow pipe, and the solenoid valve may be controlled to open and close by the level gauge; the liquid outlet of the overflow pipe discharges the liquid into the weighing pan.

[0021] The weighing pan is set on the testing platform, and an electronic weighing device is installed below the weighing pan.

[0022] Furthermore, the animal restraint device may be a bandage or may include a transverse restraint cylinder, a head restraint block, and a clamping plate; two symmetrical hind limb restraint grooves are provided at the lower end of one end of the transverse restraint cylinder; and a transverse sliding groove is provided at the upper end of one end of the transverse restraint cylinder.

[0023] The head fixing block has a central vent hole, and a sliding shaft is provided on the outer surface of the head fixing block; the sliding shaft is slidably installed in the transverse sliding groove and is provided with a locking nut;

[0024] A tightening bolt is provided above one end of the transverse fixing cylinder; the lower end of the tightening bolt is configured to rotate with the pressure plate.

[0025] A fixing device is provided on the transverse fixing cylinder; the fixing device includes a fixing plate; both ends of the fixing plate are provided with fixing rings that match the transverse fixing cylinder;

[0026] The fixed plate is provided with a hinge joint; a connector is hinged to the hinge joint by a locking bolt; a second locking nut is provided at one end of the locking bolt; the connector is detachably connected to the second telescopic bracket.

[0027] Furthermore, a first magnetic connector is rotatably connected to the second connector head;

[0028] The lower end of the second telescopic bracket is provided with a first magnetic connector; the first magnetic connector and the first magnetic joint are detachably connected.

[0029] Furthermore, the detection bracket is rotatably engaged with the lower end of the first telescopic bracket via a rotating sleeve, and a second locking bolt is provided on the rotating sleeve;

[0030] One end of the horizontal support frame is provided with a second magnetic connector, and the second magnetic connector is provided with a protrusion; one end of the detection bracket is provided with a second magnetic connector that matches the second magnetic connector; the second magnetic connector and the second magnetic connector are magnetically connected.

[0031] Furthermore, a third magnetic connector is provided at one end of the transverse support, and a clamp is provided on the lower overflow pipe; a connecting frame is provided on one side of the clamp; a third magnetic connector is provided on the connecting frame; a groove matching the third magnetic connector is provided on the third magnetic connector, and the third magnetic connector is inserted into the third magnetic connector to magnetically connect with the third magnetic connector.

[0032] Furthermore, the sliding guide rail is provided with a first linear drive device for driving the first slider to move and a second linear drive device for driving the second slider to move; both the first linear drive device and the second linear drive device are lead screw drive devices.

[0033] The beneficial effects of this invention are as follows: The animal limb measuring device with multi-positioning structure described in this invention has the following advantages:

[0034] 1. The animal limb measuring device with multi-positioning structure described in this invention, by setting a distance detection device, and setting a distance sensor in a ring array in the distance detection device, can detect animal limbs in multiple dimensions and achieve non-contact detection; it can improve the detection effect and reduce harm to the animal being detected.

[0035] 2. The animal limb measuring device with multi-positioning structure described in this invention is equipped with a segmented overflow device, and pressure sensors that are uniformly distributed longitudinally can be installed in the overflow device. Therefore, the segmented design facilitates the maintenance of the overflow device. At the same time, by detecting the gradient pressure inside the overflow device, the device can capture subtle movements of the measured limb, thereby improving the detection accuracy.

[0036] 3. The animal fixing device of the animal limb measuring device with multi-positioning structure described in this invention provides a horizontal fixing cylinder and a hinge seat above the fixing cylinder, which facilitates fixing the animal to be measured and makes it easy to adjust the position of the animal's limbs after fixing.

[0037] 4. Furthermore, the animal limb measuring device with multi-positioning structure described in this invention has its distance detection device, overflow device, and animal fixing device connected by a magnetic quick-connect device, which facilitates quick installation and replacement and is easy to operate.

[0038] In summary, the animal limb measuring device with a multi-positioning structure described in this application is easy to operate and improves detection accuracy. It enables 3D modeling through a ring array of distance sensors and allows for single, non-contact detection. The overflow device described in this application employs a segmented design and incorporates longitudinally distributed pressure sensors. The segmented design facilitates cleaning, and the uniformly distributed longitudinal pressure sensors enable gradient detection of pressure within the overflow pipe, thereby capturing subtle movements of the animal limbs and improving detection accuracy. The animal fixation device described in this application provides automatic fixation, eliminating the need for manual fixation by laboratory personnel. This ensures stability during animal experiments and enhances the laboratory personnel's focus on the experimental procedure, thus guaranteeing accurate detection. It also facilitates animal positioning and installation / removal. Attached Figure Description

[0039] Figure 1 This is a perspective view of an animal limb measuring device with a multi-positioning structure in an embodiment of the present invention;

[0040] Figure 2 This is a rear perspective view of the animal limb measuring device with a multi-positioning structure in an embodiment of the present invention;

[0041] Figure 3 This is a front view of the animal limb measuring device with a multi-positioning structure in an embodiment of the present invention;

[0042] Figure 4 This is a top view of the animal limb measuring device with a multi-positioning structure in an embodiment of the present invention;

[0043] Figure 5 yes Figure 4 AA section view;

[0044] Figure 6 This is an exploded view of an animal limb measuring device with a multi-positioning structure in an embodiment of the present invention;

[0045] Figure 7 This is a perspective view of the distance detection device in an embodiment of the present invention;

[0046] Figure 8 This is a top view of the distance detection device in an embodiment of the present invention;

[0047] Figure 9 yes Figure 8 BB cross-sectional view;

[0048] Figure 10 This is a perspective view of the distance detection device from the bottom upwards in an embodiment of the present invention;

[0049] Figure 11This is a perspective view of the overflow device in an embodiment of the present invention;

[0050] Figure 12 This is an exploded schematic diagram of the overflow device in an embodiment of the present invention;

[0051] Figure 13 This is a schematic diagram of the overflow device in an embodiment of the present invention;

[0052] Figure 14 This is an explosion diagram of the animal restraint device in an embodiment of the present invention;

[0053] Figure 15 This is a perspective view of the animal restraint device in an embodiment of the present invention;

[0054] Figure 16 This is a top view of the animal restraint device in an embodiment of the present invention;

[0055] Figure 17 yes Figure 16 CC section view;

[0056] Figure 18 This is a schematic diagram illustrating the principle of volume change through gradient pressure technology in an embodiment of the present invention;

[0057] Figure 19 This is a schematic diagram illustrating the principle of detecting animal limb volume in an embodiment of the present invention;

[0058] The diagram shows: 100-base, 200-detection platform, 300-first slider, 400-second slider, 500-distance detection device, 600-animal restraint device, 700-overflow device, 800-first linear drive device, and 900-second linear drive device. Detailed Implementation

[0059] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0060] like Figures 1 to 17 As shown, the animal limb measuring device with a multi-positioning structure of the present invention includes a base 100; a detection platform 200 is provided in the middle of the base 100.

[0061] The primary function of the testing platform 200 is to house the testing data processing module. Specifically, the testing platform 200 is made of lightweight aluminum alloy with a thickness of 2mm, and its dimensions are 30cm (length) × 20cm (width) × 40cm (height). It integrates control circuitry and a power supply module. Furthermore, a display screen for showing testing results is located on one side of the testing platform 200. The data processing module within the testing platform 200 uses an STM32H743 main control chip; specifically, the STM32H743 main control chip runs the FreeRTOS real-time operating system. The display screen is an IPS touchscreen with a resolution of 480×272, displaying volume curves and measurement parameters in real time. Additionally, the testing platform 200 incorporates a Bluetooth 5.0 chip and a CC2640R2F chip, supporting data transmission to a mobile app or PC.

[0062] The detection platform 200 has a first column 110 on one side and a second column 120 on the other side; a sliding guide rail 130 is provided on the second column 120; a first slider 300 and a second slider 400 are provided on the sliding guide rail 130.

[0063] Both the first column 110 and the second column 120 serve a supporting function, facilitating the installation of corresponding components on the first column 110 and the second column 120.

[0064] Specifically, the first column 110 and the second column 120 can both be made of stainless steel. Preferably, the first column 110 and the second column 120 are telescopic columns. Specifically, they can be electric telescopic rods, hydraulic cylinders, or pneumatic cylinders.

[0065] A first telescopic bracket 310 is provided below the first slider 300; a second telescopic bracket 410 is provided on the second slider 400; a detection bracket is provided at the lower end of the first telescopic bracket 310; a detachably connected distance detection device 500 is installed on the detection bracket;

[0066] The main function of the first slider 300 and the second slider 400 is to achieve linear movement in the horizontal direction; specifically, the first slider 300 and the second slider 400 can be moved manually or automatically by setting a corresponding driving device.

[0067] Specifically, when setting up the drive device to achieve its automatic movement, a vertical mounting plate is welded to one end of the sliding guide rail 130. A first linear drive device 800 for driving the first slider 300 to move and a second linear drive device 900 for driving the second slider 400 to move are mounted on the vertical mounting plate. Both the first linear drive device 800 and the second linear drive device 900 are lead screw drive devices.

[0068] The first linear drive device 800 includes a first drive motor and a first drive screw 810; the first drive screw 810 passes through the first slider 300 and the second slider 400, and the first drive screw 810 is threadedly engaged with the first slider 300 and slidably engaged with the second slider 400.

[0069] The second linear drive device 900 includes a second drive motor and a second drive screw 910; the second drive screw 910 passes through the first slider 300 and the second slider 400, and the second drive screw 910 is slidably engaged with the first slider 300 and threadedly engaged with the second slider 400. Both the first drive motor and the second drive motor are bolted to the vertical mounting plate.

[0070] The first column 110 has a retractable horizontal support 111 at its upper end; a detachable overflow device 700 is mounted on the horizontal support 111; the second telescopic support 410 has a detachable animal restraint device 600 at its lower end; the main function of the horizontal support 111 is to support the overflow device 700; and the horizontal position of the overflow device 700 can be adjusted by extending and retracting the horizontal support 111. The horizontal support 111 can be a telescopic column or an electrically operated telescopic rod.

[0071] The main function of the animal restraint device 600 is to secure the animal; the animal restraint device 600 can be a bandage. To facilitate automatic adjustment of the animal's position, in this embodiment, the animal restraint device 600 includes a transverse restraint cylinder 610, a head restraint block 620, and a pressure plate 690; two symmetrical hind limb restraint grooves 612 are provided at the lower end of one end of the transverse restraint cylinder 610; a transverse sliding groove 611 is provided at the upper end of one end of the transverse restraint cylinder 610.

[0072] The head fixing block 620 has a central vent hole, and a sliding shaft 621 is provided on the outer surface of the head fixing block 620; the sliding shaft 621 is slidably installed in the transverse sliding groove 611 and is provided with a locking nut 622.

[0073] A tightening bolt 630 is provided above one end of the transverse fixing cylinder 610; the lower end of the tightening bolt 630 is configured to rotate with the pressure plate 690.

[0074] A fixing device 640 is provided on the transverse fixing cylinder 610; the fixing device 640 includes a fixing plate 641; both ends of the fixing plate 641 are provided with fixing rings 642 that match the transverse fixing cylinder 610; specifically, the fixing rings 642 can be rubber rings or clamps.

[0075] A hinge joint 643 is provided on the fixed plate 641; a connector 650 is hinged to the hinge joint 643 via a locking bolt 670; a second locking nut 680 is provided at one end of the locking bolt 670; the connector 650 is detachably connected to the second telescopic bracket 410. Furthermore, a rotating connector is provided on the second connector 650; the rotating connector is detachably connected to the second telescopic bracket 410.

[0076] In this specific application, the mouse's head is placed inside the horizontal fixing cylinder 610, allowing the mouse's two hind legs to pass through the hind limb fixing groove 612. The head is fixed in position by the head fixing block 620, and the tail is pressed against the mouse's tail by tightening the top bolt 630, causing the clamping plate 690 to press against the mouse's tail. This achieves the fixation of the mouse.

[0077] When angle adjustment is required, it can be achieved by rotating the hinge joint 643 and then tightening the second locking nut 680. Simultaneously, angle adjustment can be achieved by rotating the connector head, and left / right position adjustment can be achieved via the second slider 400.

[0078] Furthermore, a forelimb fixing groove is provided at the other end of the transverse fixing cylinder 610, where a hindlimb fixing groove 612 is provided. The forelimb fixing groove is used to position the forelimbs of the mouse.

[0079] In the above embodiments, the main function of the overflow device 700 is to detect the volume of the animal's limb being tested through Archimedes' principle.

[0080] Specifically, the overflow device 700 can be an integral overflow cup with an overflow pipe on one side. The overflow pipe is introduced into a graduated collection tank, and the volume of overflowing water is read through the collection tank to detect the volume of the limb being measured. To facilitate cleaning of the overflow device 700, this embodiment uses a segmented overflow device. The overflow device 700 includes a lower overflow pipe 710 and an upper overflow pipe 720. The lower end of the lower overflow pipe 710 is provided with an inlet pipe, and a valve 711 is provided on the inlet pipe. One end of the upper overflow pipe 720 is inserted into the lower overflow pipe 710 and threadedly engaged with it. An overflow pipe 740 is provided on one side of the upper overflow pipe 720. One end of the overflow pipe 740 is connected to the upper overflow pipe 720, and an overflow liquid volume detection device is provided below the other end.

[0081] The overflow liquid volume detection device can be implemented by weighing the overflow liquid, that is, the overflow liquid volume detection device uses a weighing pan 220; the weighing pan 220 is set above the detection platform 200; an electronic weighing device 210 is set on the detection platform 200, and the weighing pan 220 is placed on the weighing device 210. A solenoid valve is installed on the overflow pipe 740; an overflow level gauge is installed in the upper overflow pipe 720, and the solenoid valve is controlled to open and close by the level gauge; the liquid outlet of the overflow pipe 740 discharges the liquid into the weighing pan 220. Specifically, during operation, the water level at which the overflow level gauge controls the solenoid valve to open is first set as the initial water level. This can be achieved by directly raising the water level to the detection position of the overflow level gauge, that is, when the overflow level gauge just touches the water level; the water level detected at this time is the original water level. Then, the animal limb to be tested is immersed in the upper overflow pipe 720. The liquid level in the upper overflow pipe 720 rises, making the water level higher than the position detected by the overflow level gauge. Then, the overflow level gauge controls the solenoid valve to open, and the overflow pipe 740 begins to drain the liquid. The drained liquid flows into the weighing pan 220. When the water level drops back to the original level, the overflow level gauge controls the solenoid valve to close. At this time, all the drained liquid enters the weighing pan 220. The volume of the drained liquid is calculated by weighing, thus obtaining the volume of the animal limb.

[0082] To simplify operation, in this embodiment, the overflow liquid volume detection device adopts a graduated vertical collection cylinder 750; the upper end of the vertical collection cylinder 750 is provided with an exhaust pipe 751; and the bottom of the vertical collection cylinder 750 is provided with an openable and closable drain pipe 752.

[0083] In practical application, the liquid level in the upper overflow pipe 720 is first brought to the same level as the overflow port of the overflow pipe 740. Then, the liquid in the vertical collection cylinder 750 is emptied through the drain pipe 752. Specifically, this can be achieved by opening the valve 711 on the lower inlet pipe of the lower overflow pipe 710 and continuously replenishing liquid into the lower overflow pipe 710 through the replenishing device until the liquid level reaches the overflow port of the overflow pipe 740. Then, replenishment is stopped, and the excess liquid in the upper overflow pipe 720 is discharged through the overflow pipe 740 into the vertical collection cylinder 750, and then emptied through the drain pipe 752 at the lower end of the vertical collection cylinder 750.

[0084] Then, close the drain pipe 752, immerse the animal limb to be tested into the liquid in the upper overflow pipe 720, and after immersing it to the marked area, wait for the liquid to overflow from the overflow pipe 740, and then read the volume data of the overflow liquid through the scale on the vertical collection cylinder 750 to obtain the volume of the animal limb to be tested.

[0085] To correct for minor movements of animal limbs during the detection process, which could cause the water level in the upper overflow pipe to rise and overflow excess liquid, this embodiment further includes pressure sensors 760 evenly distributed vertically within the upper overflow pipe 720, with the first pressure sensor 760 positioned lower than the overflow pipe 740. Specifically, an installation groove 721 is provided on the inner wall of the upper overflow pipe 720; a detachable installation block 730 is installed within the installation groove 721; and the pressure sensors 760 are mounted on the installation block 730 and evenly distributed vertically.

[0086] By setting uniformly distributed pressure sensors 760, the volume of liquid in the upper overflow pipe 720 is determined by detecting the liquid gradient pressure within the pipe. The height h is calculated using the formula P=ρgh. Since the cross-sectional area of ​​the upper overflow pipe 720 is uniformly S, the pressure detected by the uppermost pressure sensor 760 is F1=P1S. The pressures detected by the pressure sensors from top to bottom are designated as F1, F2, F3, F4…Fn. Due to the uniform distribution of pressure sensors, the distance between adjacent pressure sensors is a fixed value d. The following formula applies: ;

[0087] ;

[0088] In the formula, Fn is the pressure of the nth pressure sensor, Pn is the pressure detected by the nth sensor, and hn is the height of the water level above the nth sensor; The density of the liquid; It is the acceleration due to gravity;

[0089] like Figure 18 As shown, the x-values ​​before and after detection can be calculated:

[0090] ;

[0091] Where n is the total number of pressure sensors, and d is the vertical distance between two adjacent sensor supports.

[0092] By comparing the value of x before detection (xbefore) and the value of x after detection (xafter), the change in x, Δx, can be obtained.

[0093] The volume change is ; Where S is the cross-sectional area of ​​the upper overflow pipe 720. Therefore, the corrected volume can be obtained, i.e. .

[0094] The pressure sensor with a gradient setting detects the original volume of the upper overflow pipe 720 before detection, and the volume difference within the upper overflow pipe 720 after the animal limb is placed in the pipe and the animal is stationary. Then, the volume detected by the overflow device is subtracted... The corrected measurement volume is obtained. This allows for the revision of the detected animal limb volume, thereby eliminating detection errors caused by subtle movements of the animal limb and improving detection accuracy.

[0095] One end of the detection bracket is rotatably connected to the lower end of the first telescopic bracket 310; the distance detection device 500. The main function of the distance detection device 500 is to achieve non-contact three-dimensional detection of the limbs of the animal being tested.

[0096] To improve detection accuracy, in this embodiment, the distance detection device 500 includes a ring-shaped mounting frame 520; a range sensor 560 evenly distributed along the circumference is mounted on the ring-shaped mounting frame 520; an adjustment device 570 for adjusting the range measuring angle of the range sensor 560 is provided on the ring-shaped mounting frame 520; a ring-shaped terminal block 510 is provided above the ring-shaped mounting frame 520; a horizontal support frame 530 is provided on one side of the ring-shaped terminal block 510; a vertical support frame is provided on the horizontal support frame 530, and a camera 550 is mounted on the vertical support frame 540.

[0097] The camera 550 can observe the state of the animal's limbs in real time; the position of the animal's limbs can be adjusted according to the state of the animal's limbs so that the animal's limbs are coaxial with the array circle of the uniformly arrayed range sensors 560 along the circumference; thus, the animal's limbs are located at the center of the array range sensors 560.

[0098] During the inspection, the animal's limb moves downward under the action of the second telescopic support 410. At this time, the positional change is detected by the ranging sensor 560 of the ring array, and the data is transmitted to the data processing module within the inspection platform to achieve 3D modeling and volume detection of the animal's limb. The camera 550 enables real-time recording of the animal limb inspection process and can store the image data for easy traceability.

[0099] Specifically, the ranging sensor 560 uses a TOF (Time-of-Flight) laser sensor (VL53L0X) or a laser triangulation ranging sensor (Keyence IL-300) with an accuracy of ±0.01mm.

[0100] To facilitate the installation of the ranging sensor 560 and the adjustment of its measuring angle, in this embodiment, the annular mounting bracket 520 is further provided with a mounting groove 521 that matches the ranging sensor 560. Both sides of the ranging sensor 560 are hinged to the sidewalls of the mounting groove 521 via rotating shafts 561. The rotating shaft 561 on one side of the ranging sensor 560 extends out of the annular mounting bracket 520 and is connected to the driving device 570. Specifically, the driving device 570 is a drive motor.

[0101] When it is necessary to adjust the measuring angle, the rotating shaft 561 is driven to rotate by the drive device 570, thereby realizing the adjustment of the measuring angle.

[0102] Specifically, in the application process, firstly, the ranging sensor 560 uses a laser ranging sensor to calibrate the ranging sensor 560 in the distance detection device 500; the radius R of its circular array is determined, that is, the distance from the mounting point of the ranging sensor 560 to the center of the array circle. Then, through the zero-point calibration function, the mounting point of the ranging sensor 560 is set as a virtual reference point; at this time, the distance detected by the ranging sensor 560 is the distance relative to the mounting point of the ranging sensor 560.

[0103] Then, the animal to be tested is fixed in place using the animal restraint device 600; in this embodiment, the hind limbs of a mouse are tested. Rotating the hinge joint 643 and tightening the second locking nut 680 adjusts and fixes the animal's tilt angle. Rotating the connector on the animal restraint device 600 allows the animal to rotate around its vertical axis, adjusting the angle of the tested limb. Moving the second slider 400 adjusts the left-right position of the animal's limb, ensuring the tested limb is in a vertical position.

[0104] By rotating the detection bracket and moving the first slider 300, the animal's limbs are made coaxial with the array circle of the uniformly arrayed range sensors 560 along the circumference; thus, the animal's limbs are located at the center of the array range sensors 560.

[0105] Then, the second telescopic bracket 410 is activated to slowly extend and retract downwards at a speed v, allowing the animal's limb to slowly pass through the distance detection device 500. The distance detection device 500 is then activated for real-time detection, monitoring the entire process of the animal's limb passing through the array distance sensor 560.

[0106] During the detection process, when the real-time detection distance of at least three adjacent range sensors 560 in the array range sensor 560 is greater than 0, it is determined that the animal's limbs have begun to penetrate the array range sensor 560.

[0107] Then, data acquisition began at a fixed frequency of 100Hz, collecting distance values ​​detected in real time by six distance sensors. The data is processed by the data module set up within the detection platform 200. As shown in the figure, the radius detected by each ranging sensor 560 is calculated based on the collected real-time data. Specifically:

[0108] ;

[0109] In the formula, This represents the radius detected by the ranging sensor 560 at the corresponding time t, which is the distance from a point on the surface of the animal's limb to the center of the array. This represents the value detected by the ranging sensor 560 at the corresponding time t, where R is the radius of the circular array of the ranging sensor 560, and n is the number of the ranging sensor 560, n=1, 2, 3, 4, 5 or 6.

[0110] Calculate the cross-sectional area of ​​the animal's limbs at each moment:

[0111] In this embodiment, the circular array has six sensors, which are evenly distributed in a ring; therefore, the cross-section detected by the sensors in the array is approximately hexagonal; based on the detection radius of the sensors... The hexagon can be divided into 6 triangles with vertex angles of 60°; the area of ​​each triangle is:

[0112]

[0113] In the formula, a and b are the lengths of the two included sides of the vertex angle.

[0114] Therefore, the cross-sectional area S(t) of the hexagon is calculated using the following formula:

[0115]

[0116] In the formula, This represents the radius detected by the first ranging sensor, 560. This indicates the radius detected by the second ranging sensor 560, and so on. This indicates the radius detected by the sixth ranging sensor 560.

[0117] The detection stops when the animal's limbs have passed through the detection device at a distance of 500 meters, reaching the marked value.

[0118] Then, the volume of the animal's limbs is calculated using the data processing module. The volume V is obtained by integrating the cross-sectional area and displacement at each time point.

[0119]

[0120] In the formula, k is the number of samples. The sampling time interval is given by v, where v is the speed at which the animal's limb moves downwards. The volume of the limb cross-section is obtained during the kth data acquisition.

[0121] Furthermore, the animal's limbs can be reconstructed in three dimensions using SketchUp based on the data collected by the laser rangefinder.

[0122] After the test is completed, the distance detection device 500 is turned off, and then the second telescopic bracket 410 is retracted to remove the animal's tested limb from the distance detection device 500. Then the detection bracket is rotated so that the distance detection device 500 is moved away from under the animal fixation device 600.

[0123] Then, by adjusting the length of the transverse support 111, the position of the movable overflow device 700 is adjusted so that the upper section of the overflow pipe 720 of the overflow device 700 is basically coaxial with the limb of the animal being tested.

[0124] Then, the second telescopic bracket 410 is activated and slowly extends downward, so that the limb of the animal being tested is immersed in the upper overflow pipe 720; the volume of the animal limb is detected in the upper overflow pipe 720 by overflow method, and the specific method is as described in the working principle of the overflow device above.

[0125] Furthermore, the gradient pressure is detected by vertically and uniformly distributed pressure sensors in the upper overflow pipe 720 of the overflow device, thereby correcting the detection error. The specific working principle is described above.

[0126] In summary, the technical solution described in this application enables non-contact detection through the distance detection device 500, volume detection via overflow device 700, and correction of the detection results through gradient pressure. This improves detection accuracy. When processing the results, the volume detected by the distance detection device 500 and the volume detected by the overflow method can be weighted and fused to obtain the final result, further improving detection accuracy. The specific weighted fusion is as follows:

[0127] V = 0.7Vlaser + 0.3Voverflow; where Vlaser represents the volume detected by the ranging device; Voverflow represents the volume detected by the overflow method.

[0128] In one feasible embodiment, to facilitate quick installation and replacement, a first magnetic connector 660 is rotatably connected to the second connector 650;

[0129] The lower end of the second telescopic bracket 410 is provided with a first magnetic connector 411; the first magnetic connector 411 is detachably connected to the first magnetic connector 660.

[0130] The detection bracket is rotatably engaged with the lower end of the first telescopic bracket 310 via a rotating sleeve 320, and a second locking bolt is provided on the rotating sleeve 320. A second magnetic connector is provided at one end of the horizontal support frame 530, and the second magnetic connector has a protrusion. A second magnetic connector matching the second magnetic connector is provided at one end of the detection bracket. The second magnetic connector and the second magnetic connector are magnetically connected. A third magnetic connector 112 is provided at one end of the transverse bracket 111, and a clamp 771 is provided on the lower overflow pipe 710. A connecting frame 770 is provided on one side of the clamp 771. A third magnetic connector is provided on the connecting frame 770. A groove matching the third magnetic connector is provided on the third magnetic connector 112, and the third magnetic connector is inserted into the third magnetic connector 112, magnetically connected to the third magnetic connector 112.

[0131] That is, the animal restraint device 600, the overflow device 700, and the distance detection device 500 are all connected to the bracket by magnetic connection, which facilitates quick installation and disassembly and easy maintenance.

[0132] The animal limb measurement device with multi-positioning structure described in this application can be applied to the following fields:

[0133] 1. Research on edema and inflammation

[0134] Application: To assess limb swelling caused by trauma, infection, or drug side effects.

[0135] Experimental model:

[0136] Acute inflammation model: Carrageenan injection induces hind limb edema in rodents.

[0137] Chronic edema model: long-term swelling caused by damage to the lymphatic system or venous obstruction.

[0138] The hind limb volume of experimental mice was detected using the animal limb measurement device with a multi-positioning structure disclosed in this invention.

[0139] 2. Orthopedics and Trauma Repair

[0140] Applications: Monitoring fracture healing, bone defect repair, or tissue response around implants.

[0141] Experimental model:

[0142] Fracture model: Tibial fractures were surgically induced in mice / rat, and limb volume was measured periodically to assess callus formation and soft tissue swelling.

[0143] Bone regeneration experiments: When using biomaterials or stem cells to repair bone defects, volume changes reflect regeneration efficiency.

[0144] The volume of limbs in experimental mice was measured using the animal limb measurement device with a multi-positioning structure disclosed in this invention.

[0145] 3. Muscle diseases and sports medicine

[0146] Applications: To assess muscle atrophy (e.g., disuse atrophy), hypertrophy (e.g., exercise training), or muscular dystrophy.

[0147] Experimental model:

[0148] Muscle atrophy model: Muscle atrophy in the hind limbs of mice was induced by cast immobilization.

[0149] Editing model: Limb volume monitoring in an animal model of Duchenne muscular dystrophy (DMD).

[0150] The hind limb volume of experimental mice was detected using the animal limb measurement device with a multi-positioning structure disclosed in this invention.

[0151] 4. Oncology research

[0152] Application: To assess changes in the volume of subcutaneous xenografts (such as tumors in the limbs).

[0153] Experimental model:

[0154] Xenograft model: Human tumor cells are implanted into the hind limbs of mice, and tumor volume is measured to assess drug efficacy.

[0155] The hind limb volume of experimental mice was detected using the animal limb measurement device with a multi-positioning structure disclosed in this invention.

Claims

1. An animal limb measuring apparatus with multi-positioning structure, characterized in that: Includes a base (100); a detection platform (200) is provided in the middle of the base (100); The detection platform (200) has a first column (110) on one side and a second column (120) on the other side; a sliding guide rail (130) is provided on the second column (120); a first slider (300) and a second slider (400) are provided on the sliding guide rail (130). A first telescopic bracket (310) is provided below the first slider (300); a second telescopic bracket (410) is provided on the second slider (400); a detection bracket is provided at the lower end of the first telescopic bracket (310); a detachable distance detection device (500) is installed on the detection bracket. The first column (110) is provided with a telescopic horizontal support (111) at its upper end; the horizontal support (111) is provided with a detachable overflow device (700); the second telescopic support (410) is provided with a detachable animal fixing device (600) at its lower end. One end of the detection bracket is rotatably connected to the lower end of the first telescopic bracket (310); the distance detection device (500) includes an annular mounting bracket (520); a distance measuring sensor (560) evenly distributed along the circumference is mounted on the annular mounting bracket (520); an adjustment device (570) for adjusting the distance measuring angle of the distance measuring sensor (560) is provided on the annular mounting bracket (520); an annular junction box (510) is provided above the annular mounting bracket (520); a horizontal support frame (530) is provided on one side of the annular junction box (510); a vertical support frame is provided on the horizontal support frame (530), and a camera (550) is mounted on the vertical support frame (540); The animal restraint device (600) is used to restrain the animal being tested; the overflow device (700) is used to measure the volume of the part of the animal to be tested using the drainage method; The animal restraint device (600) includes a transverse restraint cylinder (610), a head restraint block (620), and a clamping plate (690); two symmetrical hind limb restraint grooves (612) are provided at the lower end of one end of the transverse restraint cylinder (610); a transverse sliding groove (611) is provided at the upper end of one end of the transverse restraint cylinder (610). The head fixing block (620) has a central vent hole, and a sliding shaft (621) is provided on the outer surface of the head fixing block (620); the sliding shaft (621) is slidably installed in the transverse sliding groove (611) and is provided with a locking nut (622). A tightening bolt (630) is provided above one end of the transverse fixing cylinder (610); the lower end of the tightening bolt (630) is configured to rotate with the pressure plate (690); A fixing device (640) is provided on the transverse fixing cylinder (610); the fixing device (640) includes a fixing plate (641); both ends of the fixing plate (641) are provided with fixing rings (642) that match the transverse fixing cylinder (610). The fixed plate (641) is provided with a hinge joint (643); a second connector (650) is hinged to the hinge joint (643) by a locking bolt (670); a second locking nut (680) is provided at one end of the locking bolt (670); the connector (650) is detachably connected to the second telescopic bracket (410); The overflow device (700) is a segmented overflow device; the overflow device (700) includes a lower overflow pipe (710) and an upper overflow pipe (720); the lower end of the lower overflow pipe (710) is provided with an inlet pipe; a valve (711) is provided on the inlet pipe; one end of the upper overflow pipe (720) is inserted into the lower overflow pipe (710) and is threadedly engaged with the lower overflow pipe (710); An overflow pipe (740) is provided on one side of the upper overflow pipe (720); one end of the overflow pipe (740) is connected to the upper overflow pipe (720), and an overflow liquid volume detection device is provided below the other end; The upper overflow pipe (720) is provided with pressure sensors (760) evenly distributed vertically, and the position of the first pressure sensor (760) at the upper end is lower than the position of the overflow pipe (740); An installation groove (721) is provided on the inner wall of the upper overflow pipe (720); a detachable installation block (730) is installed in the installation groove (721); the pressure sensor (760) is installed on the installation block (730) and is evenly distributed vertically. By setting up uniformly distributed pressure sensors (760), the volume of liquid in the upper overflow pipe (720) is determined by detecting the liquid gradient pressure in the upper overflow pipe (720); Non-contact detection is achieved through a distance detection device (500); the overflow device (700) is used to detect the volume by overflow method, and the results of overflow method detection are corrected by detecting the liquid gradient pressure in the upper overflow pipe (720) through uniformly distributed pressure sensors (760); the final result is obtained by weighted fusion of the volume detected by the distance detection device (500) and the volume detected by the overflow device (700) using the overflow method.

2. The animal limb measuring device with a multi-positioning structure as described in claim 1, characterized in that: The annular mounting bracket (520) is provided with a mounting groove (521) that matches the ranging sensor (560). The two sides of the ranging sensor (560) are hinged to the side wall of the mounting groove (521) through a rotating shaft (561). The rotating shaft (561) on one side of the ranging sensor (560) extends out of the annular mounting bracket (520) and is connected to the adjustment device (570) in a transmission manner.

3. The animal limb measuring device with a multi-positioning structure as described in claim 1, characterized in that: The overflow liquid volume detection device adopts a graduated vertical collection cylinder (750); an exhaust pipe (751) is provided at the upper end of the vertical collection cylinder (750); and an openable and closable drain pipe (752) is provided at the bottom of the vertical collection cylinder (750). Alternatively, the overflow liquid volume detection device may be a weighing pan (220); an electromagnetic valve may be installed on the overflow pipe (740); an overflow level gauge may be installed in the upper overflow pipe (720), and the electromagnetic valve may be controlled to open and close by the level gauge; the liquid outlet of the overflow pipe (740) may discharge the liquid into the weighing pan (220); The weighing pan (220) is set on the detection platform (200), and an electronic weighing device is set below the weighing pan (220).

4. The animal limb measuring device with a multi-positioning structure as described in claim 1, characterized in that: The second connector (650) is rotatably connected to the first magnetic connector (660); The lower end of the second telescopic bracket (410) is provided with a first magnetic connector (411); the first magnetic connector (411) is detachably connected to the first magnetic connector (660).

5. The animal limb measuring device with a multi-positioning structure as described in claim 1, characterized in that: The detection bracket is rotatably engaged with the lower end of the first telescopic bracket (310) via a rotating sleeve (320), and a second locking bolt is provided on the rotating sleeve (320); The horizontal support frame (530) is provided with a second magnetic connector at one end, and the second magnetic connector is provided with a protrusion; the detection bracket is provided with a second magnetic connector that matches the second magnetic connector at one end; the second magnetic connector and the second magnetic connector are magnetically connected.

6. The animal limb measuring device with a multi-positioning structure as described in claim 5, characterized in that: The transverse support (111) is provided with a third magnetic connector (112) at one end, and a clamp (771) is provided on the lower overflow pipe (710); a connecting frame (770) is provided on one side of the clamp (771); a third magnetic connector is provided on the connecting frame (770); a groove matching the third magnetic connector is provided on the third magnetic connector (112), and the third magnetic connector is inserted into the third magnetic connector (112) and magnetically connected with the third magnetic connector (112).

7. The animal limb measuring device with a multi-positioning structure as described in claim 1, characterized in that: The sliding guide rail (130) is provided with a first linear drive device (800) for driving the first slider (300) to move, and a second linear drive device (900) for driving the second slider to move; both the first linear drive device (800) and the second linear drive device (900) are lead screw drive devices.

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