一种提高猪体细胞核移植胚胎发育能力的方法及应用
By treating donor cells with small molecule inhibitors in porcine PEF somatic cell culture medium to reduce H3K9me3 levels, the problem of low embryonic development efficiency in porcine somatic cell nuclear transfer was solved, significantly improving blastocyst rate and reprogramming efficiency, thus overcoming the limitations of existing technologies.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NORTHEAST AGRICULTURAL UNIVERSITY
- Filing Date
- 2025-05-28
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, the developmental efficiency of porcine somatic cell nuclear transfer embryos is low, especially during the activation of the maternal zygote genome, where developmental obstacles exist. Furthermore, existing technologies such as siRNA and mRNA synthesis are costly, complex, and prone to degradation. Injection procedures cause significant damage to the embryo, thus limiting the industrial application of cloning technology.
Using porcine PEF somatic cell culture medium containing DMEM medium, fetal bovine serum, OTS186935, F5446, and SETDB1-TTD-IN-1 small molecule inhibitors, the H3K9me3 level of donor cells was reduced through culture, which improved the abnormal early developmental stage of somatic cell nuclear transfer embryos and increased reprogramming efficiency.
It significantly improved the in vitro development efficiency of porcine somatic cell nuclear transfer embryos, increased the blastocyst rate, improved reprogramming efficiency, and reduced damage to the embryos.
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Figure CN120485108B_ABST