Chromatographic purification method of recombinant adenovirus

CN120485134APending Publication Date: 2025-08-15WUHAN BOJIN BIOLOGICAL NEW MATERIALS CO LTD
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Patent Information

Application Number
CN202510663365.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2024-11-01
Filing Date
2025-05-22
Publication Date
2025-08-15

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Abstract

The present invention provides a recombinant adenovirus chromatography purification method, which comprises: taking a recombinant adenovirus-containing cell suspension, and adding a lysis agent to perform cell lysis so as to obtain a to-be-treated liquid; adding polyethylene glycol into the to-be-treated liquid, and carrying out centrifugation, resuspension and filtration to obtain a sample loading liquid; and purifying the loading solution by using a chromatographic column. According to the chromatographic purification method of the recombinant adenovirus, the loss of the adenovirus is small, and the recovery rate of the adenovirus is high.
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Citation Information

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