Non-contact optical force and temperature measurement integrated system and method

Abstract

This invention discloses a non-contact integrated system and method for photodynamic and temperature measurement, relating to the field of optical measurement and sensing technology. The system includes an excitation optical path module for providing a laser source in the 976.5nm band; a sample chamber module containing a solution of upconversion nanoparticles with highly doped lanthanide ions, the sample chamber being quickly replaceable and fixed on the sample stage; an imaging optical path module including a filter, a beam splitter, and an image acquisition device; a data processing module that calculates the potential well stiffness of the optical tweezers based on the Brownian motion trajectory of the captured particles and calculates the photodynamic force by combining the displacement offset; and obtains ambient temperature information by comparing the fluorescence intensity ratio at 520nm and 550nm bands with a pre-calibrated ratio-temperature relationship curve. This method can simultaneously acquire high-precision photodynamic and temperature information, solving the problem of single measurement in traditional biological cell measurements and achieving integrated measurement.

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