一种鸡CKAP4蛋白多克隆抗体及其应用和试剂盒

By preparing a polyclonal antibody against chicken CKAP4 protein, the shortcomings in detecting and studying CKAP4 in IBDV infection were overcome, achieving highly sensitive detection of viral infection and study of pathological mechanisms, and reducing economic losses.

CN120535624BActive Publication Date: 2026-07-17JIANGXI AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGXI AGRICULTURAL UNIVERSITY
Filing Date
2025-05-29
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The lack of effective means in the current technology to detect and study the role of CKAP4 protein in infectious bursal virus (IBDV) infection has led to insufficient research on the pathological mechanism of the disease, affecting the immune system of chicken flocks and causing economic losses.

Method used

A polyclonal antibody against chicken CKAP4 protein was prepared. High-quality nucleotide coding sequences were screened using bioinformatics. The antibody was then used to immunize New Zealand white rabbits and the serum was purified to obtain a highly sensitive and specific antibody for detecting CKAP4 protein expression and viral infection in chicken tissues.

Benefits of technology

It provides a highly sensitive and widely applicable CKAP4 protein detection method, which helps to study the pathological mechanisms of viral infections, supports IBDV detection and immune system research, and reduces economic losses.

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Abstract

本发明涉及生物技术领域,特别是涉及一种鸡CKAP4蛋白多克隆抗体及其应用和试剂盒。将CKAP4重组蛋白免疫动物,从免疫的动物中纯化血清,得到鸡CKAP4蛋白多克隆抗体,所述CKAP4重组蛋白的氨基酸序列如SEQ ID No.1所示。经过对鸡CKAP4蛋白多克隆抗体的灵敏性和特异性检测试验发现,CKAP4的血清效价为1:102400,CKAP4蛋白的表达主要集中于动物各种器官的细胞质部分,并且该多克隆抗体能特异性结合动物组织中的CKAP4蛋白。说明该多克隆抗体具有灵敏度高、特异性好、应用范围广等优势,这为后期CKAP4蛋白的检测和病毒感染类疾病病理机制的研究奠定基础。
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