A plunger type deep sea biological cultivation device for in-situ environment remodeling

Abstract

The present application relates to a kind of plunger deep-sea biological cultivation device for in-situ environment remodeling, including culture cabin, the upper portion of the culture cabin is opened with big opening, and the lower portion is opened with big opening respectively, the sidewall of culture cabin is opened with small opening, plunger is installed between two big openings, plunger is provided with multiple through ports and water inlet and outlet interface, biological transfer docking valve is installed at small opening;Flange ring is installed at the top of culture cabin, base is installed at the bottom of culture cabin, culture cabin is connected with temperature control system, realizes the zero load sealing of peripheral structure;Integrated design makes temperature adjusting coil pipe and internal medium directly contact, improves temperature adjusting efficiency;Pressure temperature compensation coil pipe eliminates the influence of medium temperature change in the process of pressurization and water exchange on temperature field in culture cabin, and comprehensive thermal management is carried out in combination with culture device work flow.

Description

Technical Field

[0001] This invention relates to the field of deep-sea organism cultivation equipment technology, and in particular to a plunger-type deep-sea organism cultivation device for in-situ environmental remodeling. Background Technology

[0002] With advancements in deep-sea exploration technology, an increasing number of deep-sea organisms are being discovered, constantly expanding our understanding. Because deep-sea organisms have adapted to the extreme deep-sea environment (pressure, temperature, etc.), conventional laboratory methods are insufficient for their cultivation. Currently, live cultivation of deep-sea organisms is achieved primarily through two methods. One is in-situ cultivation and observation in the deep-sea environment. The other is establishing a cultivation system suitable for the growth and survival of deep-sea organisms in a land-based laboratory. The first method is costly and risky; therefore, establishing deep-sea organism cultivation facilities in a land-based laboratory is one of the best solutions.

[0003] In existing technologies, cultivation devices are sealed using flange bolts, threaded connections, or external clamps, relying on the external structure of the container to bear pressure. This requires reinforced support, increases manufacturing costs, and can lead to stress concentration at bolt holes or clamp contact points, easily inducing metal fatigue and shortening service life with long-term use. Furthermore, some existing cultivation devices do not consider temperature control, making it difficult to achieve long-term low (high) temperature cultivation; some devices that do consider temperature use coils or jackets arranged externally on the chamber for temperature control, but under ultra-high pressure conditions (≥100MPa), the device wall thickness is relatively thick, making external temperature control methods inefficient; and existing cultivation devices do not consider the impact of changes in inlet water temperature due to pressurization and water changes on the internal environment of the cultivation chamber. Summary of the Invention

[0004] In response to the shortcomings of the existing production technologies, the applicant provides a plunger-type deep-sea biological cultivation device for in-situ environmental remodeling, thereby effectively solving problems such as reliance on the pressure-bearing and sealing of the outer structure of the container, low temperature regulation efficiency, and the impact of changes in inlet water temperature caused by pressurization and water exchange on the internal environment of the cultivation chamber.

[0005] The technical solution adopted in this invention is as follows:

[0006] A plunger-type deep-sea organism cultivation device for in-situ environmental remodeling includes a cultivation chamber with large openings at the top and bottom, a small opening on one side wall of the cultivation chamber, a plunger installed between the two large openings, and multiple through-holes and water inlet / outlet interfaces on the plunger. A biological transfer docking valve is installed at the small opening.

[0007] A flange ring is installed at the top of the culture chamber, and a base is installed at the bottom of the culture chamber. The culture chamber is connected to a temperature control system.

[0008] The structure of the temperature control system is as follows: It includes a refrigeration unit. The outlet of the refrigeration unit is connected to a coolant buffer tank through a pipeline. The coolant buffer tank is connected to the inlet of the refrigeration unit through a first circulation pump; the coolant buffer tank is also connected to a heat exchanger through a second circulation pump, and the heat exchanger returns to the coolant buffer tank; the coolant buffer tank is connected to the inlet of a temperature control coil through a third circulation pump. The temperature control coil is wound around the plunger, and the outlet of the temperature control coil returns and is connected to the coolant buffer tank; the heat exchanger is also connected to the water injection port of the plunger through a pipeline, and the heat exchanger is connected to the water replacement port of the plunger through a fourth circulation pump; it also includes a water tank, and the water tank is also connected in parallel with the heat exchanger through a fourth circulation pump and a pipeline, and the water tank is also connected to a pressure control system.

[0009] Its further technical solution lies in:

[0010] The culture chamber adopts an integrated structure. The main body of the culture chamber is a spherical structure, and heat insulation materials are arranged outside the culture chamber.

[0011] Multiple top screw holes and bottom threaded holes are respectively left at the top and bottom of the culture chamber.

[0012] The structure of the plunger is as follows: Upper and lower covers of the plunger are respectively provided with upper cover sealing grooves and lower cover sealing grooves. The upper cover of the plunger is provided with a water injection port for water injection and bait feeding; the upper cover of the plunger is also provided with a pressure measuring port and a temperature measuring port for installing a pressure sensor and a temperature sensor; the upper cover of the plunger is also provided with a camera penetration port and a lighting penetration port for installing a camera and a lighting lamp; screw holes are provided on the upper cover of the plunger for installing a plunger lifting lug; the lower cover of the plunger is provided with a water replacement port for water replacement and sewage discharge.

[0013] An inlet interface and an outlet interface of the temperature control coil are also reserved on the plunger for installing the temperature control coil in the temperature control system; an interface is reserved on the lower cover of the plunger for expanding functions.

[0014] The cross-sectional areas of the upper cover and the lower cover of the plunger are the same.

[0015] A plurality of first mounting holes are evenly distributed in the circumferential direction of the flange ring, and culture chamber lifting lugs are symmetrically installed on the flange ring.

[0016] The cross-section of the base is in a "convex" shape structure, and a maintenance port, a cleaning water sump, a drainage pipeline and a plurality of second mounting holes are provided on the base.

[0017] A pressurization temperature compensation coil is arranged inside the coolant buffer tank. The inlet of the pressurization temperature compensation coil is connected to the outlet of the pressure control system, and the outlet is connected to the water injection port.

[0018] A heater is provided inside the coolant buffer tank for high and low temperature adjustment.

[0019] The beneficial effects of the present invention are as follows:

[0020] This invention features a compact and rational structure, and is easy to operate. Sealing is achieved by arranging plungers at the large openings at the top and bottom of the culture chamber. The upper and lower caps of the plungers have equal cross-sectional areas, so when the chamber is pressurized, the forces on the upper and lower caps are opposite in direction and equal in magnitude. The plungers are in a self-balancing state under all pressures, achieving sealing under zero external load without requiring a supporting container structure. All necessary interfaces and functions are located on the plungers, resulting in a high degree of integration. Simultaneously, the temperature-regulating coil is arranged on the plungers; the integrated design facilitates installation and maintenance. During temperature regulation and insulation, the coil is in direct contact with the internal medium, improving temperature regulation efficiency. Furthermore, a pressure-boosting temperature compensation coil is provided, which eliminates the impact of medium temperature changes caused by pressure control system pressurization during pressurization and water changes, enabling comprehensive thermal management in conjunction with the culture device's workflow.

[0021] This invention utilizes the coordinated operation of components such as a culture chamber, plunger, flange ring, base, temperature control system, biotransfer docking valve, clamp, sealing ring, and pressure control system. The culture chamber, plunger, and sealing ring are assembled to form a bioculture space, while the remaining components facilitate device installation and use. The culture chamber has a large opening at the top and bottom, and a small opening at the equator. The plunger is inserted into the large opening of the culture chamber and has multiple through-holes for functional integration. A temperature-regulating coil is also wound around the plunger for temperature control. The upper and lower caps of the plunger have equal cross-sectional areas, maintaining self-balance under pressure conditions in the culture chamber, achieving zero-load sealing of the external structure. The integrated design allows the temperature-regulating coil to directly contact the internal medium, improving temperature regulation efficiency. The pressurization temperature compensation coil eliminates the influence of medium temperature changes during pressurization and water exchange on the temperature field inside the culture chamber, providing comprehensive thermal management in conjunction with the culture device's workflow. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of the present invention.

[0023] Figure 2 for Figure 1 Partial view (I).

[0024] Figure 3 for Figure 1 Partial view (II).

[0025] Figure 4 This is a schematic diagram of the structure of the culture chamber of the present invention.

[0026] Figure 5 This is a schematic diagram of the culture chamber of the present invention from another perspective.

[0027] Figure 6 This is a schematic diagram of the plunger structure of the present invention.

[0028] Figure 7 This is a front view of the plunger of the present invention.

[0029] Figure 8 for Figure 7 Top view.

[0030] Figure 9 for Figure 7 A bottom view.

[0031] Figure 10 This is a schematic diagram of the flange ring of the present invention.

[0032] Figure 11 This is a schematic diagram of the structure of the base of the present invention.

[0033] The components include: 1. Culture chamber; 2. Plunger; 3. Flange ring; 4. Base; 5. Temperature control system; 6. Biotransfer docking valve; 7. Top screw hole; 8. Bottom threaded hole; 9. Clamp; 10. Top cover sealing ring; 11. Bottom cover sealing ring; 12. Pressure control system.

[0034] 201. Upper cover sealing groove; 202. Lower cover sealing groove; 203. Water inlet; 204. Pressure measuring port; 205. Temperature measuring port; 206. Camera hatch; 207. Lighting hatch; 208. Screw hole; 209. Plunger lifting lug; 210. Water exchange port; 211. Temperature regulating coil inlet interface; 212. Temperature regulating coil outlet interface; 213. Reserved interface;

[0035] 301. Culture chamber lifting lug; 302. First mounting hole;

[0036] 401. Inspection port; 402. Cleaning water tank; 403. Drainage pipe; 404. Second mounting hole;

[0037] 501. Refrigeration unit; 502. Refrigerant buffer tank; 503. Heat exchanger; 504. Temperature regulating coil; 505. Insulation material; 506. First circulation pump; 507. Second circulation pump; 508. Third circulation pump; 509. Fourth circulation pump; 510. Pressure boosting temperature compensation coil; 511. Water tank; 512. First high-pressure shut-off valve; 513. Second high-pressure shut-off valve.

[0038] 50201, First inlet of buffer tank; 50202, First outlet of buffer tank; 50203, Second outlet of buffer tank; 50204, Second inlet of buffer tank; 50205, Third outlet of buffer tank; 50206, Third inlet of buffer tank; 50207, Heater. Detailed Implementation

[0039] The specific embodiments of the present invention will now be described with reference to the accompanying drawings.

[0040] like Figures 1-11As shown, the plunger-type deep-sea biological cultivation device for in-situ environmental remodeling in this embodiment includes a cultivation chamber 1. The upper and lower parts of the cultivation chamber 1 have large openings, and a small opening is opened on one side wall of the cultivation chamber 1. A plunger 2 is installed between the two large openings. The plunger 2 is provided with multiple through-holes and water inlet / outlet interfaces. A biological transfer docking valve 6 is installed at the small opening.

[0041] A flange ring 3 is installed at the top of the culture chamber 1, a base 4 is installed at the bottom of the culture chamber 1, and a temperature control system 5 is connected to the culture chamber 1.

[0042] The temperature control system 5 has the following structure: it includes a refrigeration unit 501, the outlet of which is connected to a refrigerant buffer tank 502 via a pipeline; the refrigerant buffer tank 502 is connected to the inlet of the refrigeration unit 501 via a first circulation pump 506; the refrigerant buffer tank 502 is also connected to a heat exchanger 503 via a second circulation pump 507, and the heat exchanger 503 returns the refrigerant to the refrigerant buffer tank 502; the refrigerant buffer tank 502 is connected to a temperature regulating coil 50 via a third circulation pump 508. 4. The inlet connection is provided. The temperature regulating coil 504 is wound around the plunger 2. The outlet of the temperature regulating coil 504 is returned to the refrigerant buffer tank 502. The heat exchanger 503 is also connected to the water inlet 203 of the plunger 2 through a pipe. The heat exchanger 503 is connected to the water exchange port 210 of the plunger 2 through the fourth circulation pump 509. It also includes a water tank 511, which is also connected in parallel with the heat exchanger 503 through the fourth circulation pump 509 and pipes. The water tank 511 is also connected to the pressure control system 12.

[0043] The culture chamber 1 adopts an integrated structure. The main body of the culture chamber 1 is a spherical structure, and the outside of the culture chamber 1 is covered with thermal insulation material 505.

[0044] The top and bottom of the culture chamber 1 have multiple top screw holes 7 and bottom threaded holes 8, respectively.

[0045] The structure of plunger 2 is as follows: the upper and lower covers of plunger 2 are respectively provided with an upper cover sealing groove 201 and a lower cover sealing groove 202. The upper cover of plunger 2 is provided with a water inlet 203 for water injection and baiting. The upper cover of plunger 2 is also provided with a pressure measuring port 204 and a temperature measuring port 205 for installing pressure sensors and temperature sensors. The upper cover of plunger 2 is also provided with a camera hatch 206 and a lighting hatch 207 for installing cameras and lighting lights. The upper cover of plunger 2 is provided with screw holes 208 for installing plunger lifting lugs 209. The lower cover of plunger 2 is provided with a water exchange port 210 for water exchange and sewage discharge.

[0046] The plunger 2 also has a reserved inlet interface 211 and an outlet interface 212 for the temperature control coil 504 in the temperature control system 5; the lower cover of the plunger 2 has a reserved interface 213 for expanding functions.

[0047] The upper and lower covers of plunger 2 have the same cross-sectional area.

[0048] The flange ring 3 has multiple first mounting holes 302 evenly distributed in the circumferential direction, and the culture chamber lifting lugs 301 are symmetrically installed on the flange ring 3.

[0049] The base 4 has a "convex" shaped cross-section and is equipped with an inspection port 401, a cleaning water tank 402, a drainage pipe 403, and multiple second mounting holes 404.

[0050] The refrigerant buffer tank 502 is equipped with a pressurization temperature compensation coil 510. The inlet of the pressurization temperature compensation coil 510 is connected to the outlet of the pressure control system 12, and the outlet is connected to the water inlet 203.

[0051] The refrigerant buffer tank 502 is equipped with a heater 50207 for high and low temperature regulation.

[0052] like Figures 1-3 As shown in the figure, the specific structure and function of a plunger-type deep-sea organism cultivation device for in-situ environment remodeling in this embodiment are as follows:

[0053] It includes a culture chamber 1, the main body of which is a spherical structure. A large opening is provided at the top and bottom of the culture chamber 1, and a small opening is provided at the equator of the spherical shell of the culture chamber 1.

[0054] The culture chamber 1 has plungers 2 installed at the large openings at the top and bottom. The plungers 2 are equipped with multiple through-holes and water inlet / outlet interfaces.

[0055] A flange ring 3 is installed on the top of the culture chamber 1. The flange ring 3 is symmetrically provided with culture chamber lifting lugs 301 and first mounting holes 302.

[0056] The bottom of the culture chamber 1 is equipped with a base 4, which has an inspection port 401, a cleaning water tank 402, a drainage pipe 403 and multiple second mounting holes 404.

[0057] The culture chamber 1 is connected to a temperature control system 5, which includes a refrigeration unit 501, a refrigerant buffer tank 502, a heat exchanger 503, a temperature regulating coil 504, insulation material 505, a first circulation pump 506, a second circulation pump 507, a third circulation pump 508, a fourth circulation pump 509, a pressurization temperature compensation coil 510, and a water tank 511.

[0058] The small opening at the equator of culture chamber 1 is connected to a biotransfer docking valve 6.

[0059] The top and bottom of the culture chamber 1 have multiple top screw holes 7 and bottom threaded holes 8, respectively.

[0060] like Figure 6 and Figure 7As shown, plunger 2 has a cylindrical I-shaped structure. The upper and lower covers of plunger 2 are respectively provided with an upper cover sealing groove 201 and a lower cover sealing groove 202. The upper cover of plunger 2 has a water inlet 203 for water injection and baiting. The upper cover of plunger 2 also has a pressure measuring port 204 and a temperature measuring port 205 for installing pressure and temperature sensors. The upper cover of plunger 2 also has a camera hatch 206 and a lighting hatch 207 for installing cameras and lights. The upper cover of plunger 2 has screw holes 208 for installing plunger lifting lugs 209. The lower cover of plunger 2 has a water exchange port 210 for water exchange and sludge discharge. The plunger 2 also has a reserved inlet interface 211 and an outlet interface 212 for a temperature regulating coil for installing the temperature regulating coil 504 in the temperature control system 5. The lower cover of plunger 2 has a reserved interface 213 for expanding functionality.

[0061] like Figure 4 As shown, two culture chamber lifting lugs 301 are arranged at 180° intervals on the flange ring 3, and six first mounting holes 302 are arranged at 60° intervals. The flange ring 3 is connected to the culture chamber 1 by mounting screws in the first mounting holes 302.

[0062] like Figure 1 As shown, the biotransfer docking valve 6 is connected to the culture chamber 1 via clamp 9. Clamp 9 consists of two halves and is assembled and disassembled by bolts.

[0063] like Figure 1 , Figure 3 As shown, in the temperature control system 5, the outlet of the refrigeration unit 501 is connected to the first inlet 50201 of the refrigerant buffer tank 502 via a pipeline. The first outlet 50202 of the refrigerant buffer tank 502 is connected to the inlet of the refrigeration unit 501 via a first circulating pump 506. The second outlet 50203 of the refrigerant buffer tank 502 is connected to the primary side inlet of the heat exchanger 503 via a second circulating pump 507. The outlet 50204 is connected to the primary side outlet of the heat exchanger 503 via a pipeline; the third outlet 50205 of the refrigerant buffer tank 502 is connected to the inlet of the temperature regulating coil 504 via the third circulation pump 508, and the third inlet 50206 of the refrigerant buffer tank 502 is connected to the outlet of the temperature regulating coil 504 via a pipeline; the secondary side outlet of the heat exchanger 503 is connected to the water inlet 203 via a pipeline, and the secondary side inlet is connected to the water exchange outlet 210 via the fourth circulation pump 509.

[0064] like Figure 6 As shown, the cross-sectional areas of the upper and lower covers of plunger 2 are equal.

[0065] The upper cover sealing groove 201 and the lower cover sealing groove 202 on the plunger 2 are sealed with upper cover sealing ring 10 and lower cover sealing ring 11. The inner wall of the culture chamber 1, the plunger 2, the upper cover sealing ring 10 and the lower cover sealing ring 11 are assembled to form a sealed space.

[0066] like Figure 1 As shown, the temperature control system 5 is also equipped with a water tank 511. The water tank 511 is also connected in parallel with the heat exchanger 503 through the fourth circulation pump 509 and pipelines. The fourth circulation pump 509 is used to heat up and cool the water in the water tank 511. The water tank 511 is also connected to the pressure control system 12 to supply hot and cold water for pressurization and water exchange.

[0067] The temperature control system 5 is also equipped with a pressurization temperature compensation coil 510 placed in the refrigerant buffer tank 502. The inlet of the pressurization temperature compensation coil 510 is connected to the outlet of the pressure control system 12, and the outlet is connected to the water inlet 203.

[0068] like Figure 1 , Figure 3 As shown, the coolant buffer tank 502 is equipped with a heater 50207 for high and low temperature regulation; the temperature regulating coil 504 is spirally wound on the plunger 2 and is a pressure-resistant coil; the outside of the culture chamber 1 is covered with heat insulation material 505.

[0069] The working principle and actual implementation process of this embodiment are as follows:

[0070] This embodiment achieves sealing by installing a plunger 2 inside the culture chamber 1, with the upper and lower covers of the plunger 2 having the same cross-sectional area. When the culture chamber 1 is pressurized, the upper and lower covers of the plunger 2 are subjected to equal and opposite forces, ensuring that the plunger 2 is in a self-balancing state under various pressures. This eliminates the need for external loads from the external structure to achieve a seal between the culture chamber 1 and the plunger 2. The plunger 2 is inserted into the culture chamber 1, and various interfaces can be integrated on it, along with a temperature-regulating coil 504. This allows direct contact between the medium inside the culture chamber 1 and the temperature-regulating coil 504, improving heat transfer and insulation efficiency. The water tank 511 provides hot and cold water for pressurization and water exchange. However, the pressurization process causes temperature changes in the medium. Before entering the culture chamber 1, the pressurized medium undergoes temperature regulation through heat exchange with the refrigerant via a pressurization temperature compensation coil 510, ensuring the medium enters the culture chamber 1 at the target temperature. This eliminates the impact of pressurization and water exchange on the temperature field inside the culture chamber 1.

[0071] In actual work process:

[0072] First, install and fix the culture chamber 1 to the base 4 and install the insulation material 505. Install the upper cover sealing ring 10 and the lower cover sealing ring 11 into the upper cover sealing groove 201 and the lower cover sealing groove 202, respectively. Then, install the temperature regulating coil 504 onto the plunger 2, connecting the coil inlet and outlet to the temperature regulating coil inlet interface 211 and the temperature regulating coil outlet interface 212 on the plunger 2, respectively. Install the pressure sensor, temperature sensor, camera, and lighting at the pressure measuring port 204, temperature measuring port 205, camera penetration port 206, and lighting penetration port 207 on the plunger 2, respectively. Then, lower the plunger 2 into the culture chamber 1 using the plunger lifting lug 209, install and fix the flange ring 3 to the culture chamber 1, and connect the temperature control system 5 to the culture chamber 1 according to the pipeline. Next, inject water into the culture chamber 1 through the water inlet 203, turn on the temperature control system 5 and set the target temperature to rapidly cool or heat the refrigerant. After the culture chamber 1 is filled with water, the second circulation pump 507, the third circulation pump 508, and the fourth circulation pump 509 are turned on. The medium in the culture chamber 1 exchanges heat with the refrigerant through the heat exchanger 503 and the temperature regulating coil 504. After the temperature is adjusted to the target temperature, the external atmospheric pressure pipeline is cut off by the first high-pressure shut-off valve 512 and the second high-pressure shut-off valve 513, stopping the internal circulation of the culture chamber 1. The fourth circulation pump 509 is then connected to the water tank 511, and the water in the water tank 511 is heated and cooled to supply pressurization and water replacement. The third circulation pump 508 is kept open, and the temperature regulating coil 504 is used to neutralize the loss of heat to the environment. Then, water is drawn from the water tank 511 and pumped into the culture chamber 1 through the pressure control system 12 for pressurization. Before entering the culture chamber 1, the pressurized medium passes through the pressurization temperature compensation coil 510 to eliminate the temperature change of the medium during the pressurization process. After the target environment is reached, the organisms to be cultured are transferred to the culture chamber 1 for long-term cultivation through the biotransfer docking valve 6. During the cultivation period, feeding and water exchange are carried out through water inlet 203 and water exchange outlet 210, and the temperature is maintained stable through temperature control coil 504.

[0073] In this embodiment, plungers 2 are arranged at the large openings at the top and bottom of the culture chamber to achieve sealing. The upper and lower covers of the plunger 2 have equal cross-sectional areas. When the chamber is pressurized, the upper and lower covers of the plunger 2 experience forces in opposite directions and of equal magnitude. The plunger 2 is in a self-balancing state under all pressures. The plunger 2 achieves sealing under zero external load conditions, without the need for a container structure to support it. All necessary interfaces and functions inside the device are located on the plunger 2, resulting in a high degree of device integration. At the same time, the temperature regulating coil 504 is arranged on the plunger 2. The integrated design facilitates installation and maintenance. During temperature regulation and heat preservation, the coil is in direct contact with the internal medium, improving temperature regulation efficiency. In addition, a pressure boosting temperature compensation coil 510 is provided. During pressurization and water change, the influence of medium temperature changes caused by the pressure control system 12 pressurization can be eliminated, and comprehensive thermal management is carried out in conjunction with the workflow of the culture device.

[0074] The above description is an explanation of the present invention and not a limitation thereof. The scope of the present invention is defined by the claims. Within the scope of protection of the present invention, any form of modification may be made.

Claims

1. A plunger-type deep-sea organism cultivation device for in-situ environment remodeling, characterized in that: The culture chamber (1) includes a culture chamber (1), with large openings in the upper and lower parts, and a small opening in one side wall of the culture chamber (1). A plunger (2) is installed between the two large openings. The plunger (2) is provided with multiple through-holes and water inlet / outlet interfaces. A biological transfer docking valve (6) is installed at the small opening. A flange ring (3) is installed at the top of the culture chamber (1), a base (4) is installed at the bottom of the culture chamber (1), and a temperature control system (5) is connected to the culture chamber (1). The temperature control system (5) has the following structure: it includes a refrigeration unit (501), the outlet of which is connected to a refrigerant buffer tank (502) via a pipeline, and the refrigerant buffer tank (502) is connected to the inlet of the refrigeration unit (501) via a first circulation pump (506); the refrigerant buffer tank (502) is also connected to a heat exchanger (503) via a second circulation pump (507), and the heat exchanger (503) returns to the refrigerant buffer tank (502); the refrigerant buffer tank (502) is connected to the inlet of a temperature regulating coil (504) via a third circulation pump (508), and the temperature regulating coil (504) is wound around the plunger (2) to regulate the temperature. The outlet of the coil (504) is returned to the refrigerant buffer tank (502); the heat exchanger (503) is also connected to the water inlet (203) of the plunger (2) through a pipe, and the heat exchanger (503) is connected to the water exchange inlet (210) of the plunger (2) through the fourth circulation pump (509); it also includes a water tank (511), which is also connected in parallel with the heat exchanger (503) through the fourth circulation pump (509) and pipes, and the water tank (511) is also connected to the pressure control system (12); the culture chamber (1) adopts an integrated structure, the main body of the culture chamber (1) is a spherical structure, and the outside of the culture chamber (1) is covered with heat insulation material (505). The structure of the plunger (2) is as follows: the upper and lower covers of the plunger (2) are respectively provided with an upper cover sealing groove (201) and a lower cover sealing groove (202); the upper cover of the plunger (2) is provided with a water inlet (203) for water injection and feeding; the upper cover of the plunger (2) is also provided with a pressure measuring port (204) and a temperature measuring port (205) for installing pressure sensors and temperature sensors; the upper cover of the plunger (2) is also provided with a camera hatch (206) and a lighting hatch (207) for installing cameras and lighting lamps; the upper cover of the plunger (2) is provided with screw holes (208) for installing plunger lifting lugs (209); the lower cover of the plunger (2) is provided with a water exchange port (210) for water exchange and sewage discharge; The plunger (2) also has a reserved inlet interface (211) and an outlet interface (212) for the temperature control coil (504) in the temperature control system (5); the lower cover of the plunger (2) has a reserved interface (213) for expanding the function; The upper and lower covers of the plunger (2) have the same cross-sectional area; The refrigerant buffer tank (502) is equipped with a pressure-boosting temperature compensation coil (510). The inlet of the pressure-boosting temperature compensation coil (510) is connected to the outlet of the pressure control system (12), and the outlet is connected to the water inlet (203).

2. The plunger-type deep-sea organism cultivation device for in-situ environment remodeling as described in claim 1, characterized in that: The culture chamber (1) has multiple top screw holes (7) and bottom threaded holes (8) at the top and bottom, respectively.

3. The plunger-type deep-sea organism cultivation device for in-situ environment remodeling as described in claim 1, characterized in that: The flange ring (3) has multiple first mounting holes (302) evenly distributed in the circumferential direction, and culture chamber lifting lugs (301) are symmetrically installed on the flange ring (3).

4. The plunger-type deep-sea organism cultivation device for in-situ environment remodeling as described in claim 1, characterized in that: The base (4) has a "convex" shaped cross-section and is provided with an inspection port (401), a cleaning water tank (402), a drainage pipe (403) and multiple second mounting holes (404).

5. The plunger-type deep-sea organism cultivation device for in-situ environment remodeling as described in claim 1, characterized in that: The refrigerant buffer tank (502) is equipped with a heater (50207) for high and low temperature regulation.

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