Insecticidal composition and use thereof

Abstract

The application belongs to the technical field of pesticide insecticide, and discloses a kind of insecticide composition and its application, the insecticide composition includes active ingredient A and active ingredient B, the active ingredient A is formula I compound: the active ingredient B is any one of formula II compound: or formula III compound:;The mass ratio of the active ingredient A and the active ingredient B is 1:50~50:1.The insecticide composition of the application can form complementary effect by compounding insecticides with different action mechanisms, improve the control effect, increase the difficulty of pest adaptation, and delay the development of drug resistance.Through compounding, "one drug multi-treatment" is realized, and the number of pesticide application is reduced.

Description

Technical Field

[0001] This invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition and its application. Background Technology

[0002] Compound I is a neonicotinoid insecticide developed by Meiji Seika Pharmaceutical Co., Ltd. of Japan. Its chemical name is [N(E)]-N-[1-[(6-chloro-3-pyridyl)methyl]-2(1H)-pyridinyl]-2,2,2-trifluoroacetamide. This agent acts on the nicotinic acetylcholine receptor (nAChR) and is effective against various pests, including those in the orders Hemiptera, Lepidoptera, Thysanoptera, and Diptera. Its structural formula is as follows:

[0003]

[0004] Compound II is a novel insecticide with an arylalkoxypyrimidine structure developed by Nippon Nippon Agricultural Chemicals Co., Ltd. Its chemical name is 5-(1,3-dioxane-2-yl)-4-{[4-(trifluoromethyl)benzyl]oxy}pyrimidine. It possesses a novel mechanism of action, reducing the pest population by inhibiting larval molting. Its structural formula is shown below:

[0005]

[0006] Compound III is an isoxazoline compound, belonging to the GABA-gated chloride channel allosteric modulators. This compound has a broad insecticidal spectrum, low toxicity, and high efficacy. The structure of compound III is shown below:

[0007]

[0008] Hemiptera pests threaten agricultural production through a triple mechanism of direct feeding, virus transmission, and secondary diseases. As a significant threat to agriculture and forestry, both adults and nymphs of hemiptera possess piercing-sucking mouthparts, directly robbing nutrients from plant sap, leading to stunted growth, deformities, and even death of crops. Typical examples include aphids and planthoppers. Aphids congregate to suck sap from young leaves, causing sooty mold and transmitting cucumber mosaic virus, resulting in yield reductions of over 30%. Planthoppers (such as rice planthoppers) pierce and suck sap from the phloem of rice, transmitting viruses such as rice black-streaked dwarf virus, leading to "whiteheads" and crop failure, with annual losses exceeding tens of billions of yuan. These pests have extremely high reproductive rates (aphids, for example, have a generation time of 10-20 days) and easily develop resistance to pesticides, posing a serious challenge to traditional chemical control. Furthermore, the honeydew secreted by hemiptera pests can induce sooty mold, disrupt photosynthesis, and act as virus vectors, causing indirect losses far exceeding the damage from direct feeding. Breakthroughs are urgently needed in the control technology for this type of pest. This invention studies the rational combination of compounds of formula I with those of formula II or III based on the control research of this type of pest, providing an important direction for the development of new and highly effective control agents. Summary of the Invention

[0009] Based on the above, the purpose of this invention is to provide an insecticidal composition and its application. This insecticidal composition is mainly used to control hemiptera pests, and is particularly effective against planthoppers and aphids. This insecticidal composition combines insecticides with different mechanisms of action to form a complementary effect, improve the control effect, increase the difficulty for pests to adapt, delay the development of drug resistance, and achieve "one drug for multiple treatments" through reasonable compounding, thereby reducing the number of times pesticides are applied.

[0010] To achieve the above objectives, the present invention adopts the following technical solution: an insecticidal composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: The active ingredient B is a compound of formula II: Or compounds of formula III: Any one of them; the mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1.

[0011] Furthermore, the active ingredient B is a compound of formula II, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 30:1;

[0012] The active ingredient B is a compound of formula III, and the mass ratio of active ingredient A to active ingredient B is 1:40 to 30:1.

[0013] Furthermore, the active ingredient B is a compound of formula II, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 10:1;

[0014] The active ingredient B is a compound of formula III, and the mass ratio of active ingredient A to active ingredient B is 1:10 to 20:1.

[0015] Furthermore, based on the total mass of the insecticidal composition (100 wt%), the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 1 to 80 wt%.

[0016] Furthermore, the insecticidal composition includes auxiliary components in addition to the active ingredients, and the auxiliary components are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

[0017] Furthermore, the insecticidal composition can be prepared into any agriculturally permissible formulation, which may be a solid formulation, a liquid formulation, or a seed treatment formulation.

[0018] Furthermore, the dosage form is either a solid dosage form or a liquid dosage form.

[0019] Furthermore, the solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension.

[0020] The present invention also discloses the use of the insecticidal compositions and / or their formulations as described above in the control of pests in agriculture and / or other fields.

[0021] Furthermore, the pests mentioned are hemiptera pests, specifically aphids and planthoppers.

[0022] To achieve the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application location, application method, and formulation used.

[0023] The beneficial effects of this invention are as follows:

[0024] The insecticidal composition of the present invention combines insecticides with different mechanisms of action to form a complementary effect, resulting in a significant synergistic effect, improving the control effect, increasing the difficulty for pests to adapt, delaying the development of drug resistance, and achieving "one drug for multiple treatments" through compounding, thus reducing the number of applications. Detailed Implementation

[0025] To better understand the essence of the present invention, the following embodiments further illustrate the content of the present invention, but these should not be regarded as limitations on the present invention. The content mentioned in the embodiments is not a limitation of the present invention, and the selection of material formulations can be adapted to local conditions without having a substantial impact on the results.

[0026] Formulation preparation method:

[0027] 1. Suspension preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0028] 2. Preparation method of emulsifiable concentrate: According to the formula ratio, add the measured active ingredients, solvent and co-solvent into the mixing tank and stir to dissolve them. Then add the emulsifier, and use the remaining solvent to make up the balance. Stir evenly in the mixing tank, and filter to obtain the emulsifiable concentrate required by the present invention.

[0029] 3. Preparation method of dispersible oil suspension: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0030] 4. Preparation method of water-dispersible granules: According to the formulation ratio of the example, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet pulverization, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0031] 5. Preparation method of wettable powder: According to the formula ratio, the active ingredients, dispersant, wetting agent and filler are mixed and stirred evenly in a mixer. After being pulverized by an air jet mill, they are mixed evenly again to prepare the wettable powder of the composition of the present invention.

[0032] Formulation preparation example:

[0033] Preparation Example 1: 24% Formula I compound · Formula II compound suspension (5:1)

[0034] By weight percentage, 20% of Formula I compound, 4% of Formula II compound, 3% styrene-based phenol polyoxyethylene ether, 5% glycerol fatty acid ester polyoxyethylene ether, 2% succinate sulfonate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.1% isothiazolinone, 0.5% silicone oil, and deionized water to make up the balance.

[0035] Preparation Example 2: 24% Formula I and Formula III compound suspension (1:5)

[0036] By weight percentage, 4% of Formula I compound, 20% of Formula III compound, 2% of Gelbert alcohol polyoxyethylene ether, 2% sodium dodecyl sulfate, 2% glycerol fatty acid ester polyoxyethylene ether, 1% sodium lignosulfonate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.5% silicone oil, 0.5% sodium sorbate, and deionized water to make up the balance.

[0037] Preparation Example 3: 18% Emulsifiable Oil of Formula I and Formula II (1:1)

[0038] By weight percentage, 9% of Formula I compound, 9% of Formula II compound, 8% DMF, 10% ethylene glycol oxyethylene polyoxypropylene ether, 4% sodium dodecyl sulfate, 12% propylene carbonate, and xylene to make up the balance.

[0039] Preparation Example 4: 11% emulsifiable concentrate of Formula I and Formula III (1:10)

[0040] By weight percentage, 1% of Formula I compound, 10% of Formula III compound, 20% of N-methylpyrrolidone, 5% phenylethylphenol polyoxyethylene polyoxypropylene ether, 2% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 2% sodium alkyl polyoxyethylene ether sulfonate, 10% DMF, and methyl oleate to make up the balance.

[0041] Preparation Example 5: 24% Formula I / Formula II Dispersible Oil Suspension (1:5)

[0042] By weight percentage, 4% of Formula I compound, 20% of Formula II compound, 5% sodium polycarboxylate, 10% phenylethylphenol polyoxyethylene polyoxypropylene ether, 2% calcium dodecylbenzenesulfonate, 1% sodium lignosulfonate, and soybean oil to make up the balance.

[0043] Preparation Example 6: 22% Formula I / Formula III Dispersible Oil Suspension (10:1)

[0044] By weight percentage, 20% of Formula I compound, 2% of Formula III compound, 5% of dehydrated sorbitan oleate polyoxyethylene ether, 5% tristyrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 1% sodium polycarboxylate, 1% styrylphenol polyoxyethylene ether sulfate, and soybean oil to make up the balance.

[0045] Preparation Example 7: 22% aqueous dispersible granules of Formula I and Formula II compounds (1:10)

[0046] By weight percentage, 2% of Formula I compound, 20% of Formula II compound, 3% of naphthalene sulfonate formaldehyde condensate, 8% of alkyl polyoxyethylene ether sulfonate sodium, 4% of dodecylbenzene sulfonate sodium, 3% silica, 8% ammonium sulfate, and kaolin to make up the balance.

[0047] Preparation Example 8: 36% aqueous dispersible granules of Formula I and Formula III (5:1)

[0048] By weight percentage, 30% of Formula I compound, 6% of Formula III compound, 10% sodium dodecyl sulfate, 3% styrene-phenol polyoxyethylene ether sulfate, 5% pull-out powder BX, 5% ammonium sulfate, 2% sodium dodecyl sulfate, and kaolin to make up the balance.

[0049] Preparation Example 9: 48% wettable powder of Formula I and Formula II compounds (15:1)

[0050] By weight percentage, 45% of Formula I compound, 3% of Formula II compound, 8% styrene-phenol polyoxyethylene ether sulfate, 6% alkylphenol-formaldehyde resin polyoxyethylene ether sulfate, 3% pull-out powder BX, 10% ammonium sulfate, and kaolin to make up the balance.

[0051] Preparation Example 10: 21% wettable powder of Formula I and Formula III compounds (20:1)

[0052] By weight percentage, 20% of Formula I compound, 1% of Formula III compound, 3% naphthalene sulfonate formaldehyde condensate, 2% sodium polycarboxylate, 10% dispersant NNO, 5% silica, 12% starch, and kaolin to make up the balance.

[0053] Example 1: Indoor bioactivity test of compound I and compound II against alfalfa aphids

[0054] Test reagents: Formula I and Formula II technical materials. Based on the preliminary test results, five series concentrations were set according to the proportional method during the formal test. The technical materials were dissolved and diluted with a suitable solvent to prepare a stock solution of a certain concentration. Before the test, the stock solution was further diluted proportionally with a 0.1% (v / v) Tween-80 aqueous solution.

[0055] The insect source used for testing was alfalfa aphids (nymphs).

[0056] Experimental Method: Leaf-disc spraying method. Filter paper was placed in a 9cm diameter petri dish, and an appropriate amount of distilled water was added to moisten the filter paper, but without standing water. Air between the filter paper and the petri dish was removed, ensuring the filter paper adhered tightly to the bottom of the dish. A moistened cotton ball was then placed in the dish to maintain humidity. Alfalfa leaves were punched into leaf discs, with two leaf discs placed in each dish. Selected alfalfa aphids of uniform growth, raised indoors, were introduced onto the leaf discs, 30 aphids per dish, with each dish constituting one replicate. Each concentration treatment was replicated four times. During treatment, the petri dishes were placed in the bottom tray of a Potter spray tower for spraying, with a spray volume of 1mL. After the solution settled for 1 minute, it was removed. The control was treated with the same amount of water. The petri dishes were incubated at (25±1)℃, relative humidity of 70%±5%, and a light-dark cycle of 16h:8h. After 48h, the mortality rate was checked. The insects were gently touched with the tip of a brush; those that did not move or were stiff were considered dead.

[0057] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0058]

[0059] In the formula:

[0060] P – Mortality rate, expressed as a percentage (%);

[0061] K represents the number of dead insects, in heads;

[0062] N represents the total number of insects treated, in units of heads.

[0063]

[0064] In the formula:

[0065] P1—Adjusted mortality rate, in percentage (%);

[0066] Pt —The mortality rate is expressed as a percentage (%).

[0067] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0068] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0069] The toxicity regression equation, correlation coefficient, and LC were obtained using a statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0070] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0071]

[0072] In the formula:

[0073] ATI – Actual Measured Toxicity Index of Mixtures;

[0074] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0075] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0076] TTI = TI A ×P A +TI B ×P B

[0077] In the formula:

[0078] TTI – Theoretical Toxicity Index of Mixtures;

[0079] TI A —A. Toxicity index of drug A;

[0080] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0081] TI B —Toxicity index of drug B;

[0082] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0083]

[0084] In the formula:

[0085] CTC – Cotoxicity Coefficient;

[0086] ATI – Actual Measured Toxicity Index of Mixtures;

[0087] TTI – Theoretical Toxicity Index of Mixtures.

[0088] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0089] Table 1. Results of indoor bioactivity tests on alfalfa aphids using compounds of formula I and formula II.

[0090]

[0091]

[0092] Indoor experiments showed that the combination of compound I and compound II in an appropriate mass ratio significantly enhanced the efficacy against aphids. A mass ratio of 1:30 to 30:1 between compound I and compound II resulted in a co-toxicity coefficient greater than 120, indicating a synergistic effect. A mass ratio of 1:20 to 10:1 between compound I and compound II resulted in a co-toxicity coefficient greater than 130, demonstrating a significant synergistic effect. A mass ratio of 1:5 to 10:1 between compound I and compound II resulted in a co-toxicity coefficient greater than 140, indicating a remarkable synergistic effect.

[0093] Example 2: Indoor bioactivity test of compound I and compound III on rice planthopper

[0094] Experimental target: Brown planthopper (Nilaparvata lugens), 3rd instar nymph.

[0095] Test reagents: Formula I compound technical grade, Formula III compound technical grade; dissolve the test reagents in a suitable solvent to prepare a stock solution of a certain concentration, and then dilute the stock solution with 0.1% Tween-80 aqueous solution to 5 series.

[0096] Experimental method: The pressure of the Potter spray tower was stabilized at 1.47 × 10⁻⁶. 5Pa, the spray nozzle was first cleaned twice with acetone, then twice with distilled water. Forty test insects of uniform physiological condition were selected using a brush and placed in a culture dish. The dish was then placed on the bottom of the Potter spray tower for quantitative spraying, with a spray volume of 1 mL. After the solution settled for 1 minute, it was removed. Each treatment was repeated four times, with a 0.1% Tween-80 aqueous solution used as a blank control. The treated insects were then placed in an intelligent artificial climate chamber with a temperature of (25±1)℃, a relative humidity of 60%–80%, and a photoperiod of 16L:8D. Mortality was checked after 48 hours; insects that did not move at all when gently touched with a No. 0 brush were considered dead.

[0097] Calculation method:

[0098] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0099]

[0100] In the formula:

[0101] P – Mortality rate, expressed as a percentage (%);

[0102] K represents the number of dead insects, in heads;

[0103] N represents the total number of insects treated, in units of heads.

[0104]

[0105] In the formula:

[0106] P1 – Corrected mortality rate, in percentage (%);

[0107] P t —The mortality rate is expressed as a percentage (%).

[0108] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0109] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0110] The toxicity regression equation, correlation coefficient, and LC were obtained using a statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0111] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0112]

[0113] In the formula:

[0114] ATI – Actual Measured Toxicity Index of Mixtures;

[0115] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0116] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0117] TTI = TI A ×P A +TI B ×P B

[0118] In the formula:

[0119] TTI – Theoretical Toxicity Index of Mixtures;

[0120] TI A —A. Toxicity index of drug A;

[0121] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0122] TI B —Toxicity index of drug B;

[0123] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0124]

[0125] In the formula:

[0126] CTC – Cotoxicity Coefficient;

[0127] ATI – Actual Measured Toxicity Index of Mixtures;

[0128] TTI – Theoretical Toxicity Index of Mixtures.

[0129] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0130] The results of the indoor tests are shown in the table below:

[0131] Table 2. Results of indoor bioactivity tests on planthoppers using compounds of Formula I and Formula III.

[0132]

[0133] Indoor experiments showed that the combination of compound I and compound III in an appropriate mass ratio significantly enhanced the efficacy against brown planthopper in rice. A mass ratio of 1:40 to 30:1 resulted in a co-toxicity coefficient greater than 120, indicating a synergistic effect. A mass ratio of 1:10 to 20:1 resulted in a co-toxicity coefficient greater than 140, demonstrating a significant synergistic effect. A mass ratio of 1:10 to 10:1 resulted in a co-toxicity coefficient greater than 150, indicating a remarkable synergistic effect.

[0134] Example 3: Field efficacy test for controlling peach aphids

[0135] Experimental site: Peach orchard in Xima Village, Danianchen Town, Huimin County, Binzhou City, Shandong Province. The soil is sandy loam, the peach trees are 15 years old, the soil is moderately fertile, and irrigation and drainage are convenient.

[0136] Experimental target: Peach aphid.

[0137] Experimental design: The experiment set up 6 treatments, including 5 drug treatments and 1 water control. Each treatment was repeated 4 times, and each plot was arranged in a randomized block design.

[0138] Experimental methods: The experiment was conducted in May 2024 using a MATABI Super Green 16 backpack sprayer with a nozzle diameter of 0.8 mm and a working pressure of 2.0 Bar. The entire plant was sprayed evenly, and the amount of pesticide applied was such that the pesticide was evenly applied to both sides of the leaves with a little dripping. No other insecticides were applied during the experiment.

[0139] Survey Methods: Random sampling was conducted, with 3 trees surveyed per treatment area. From each tree, 10 leaves were selected from 5 short shoots, and the total number of aphids on 150 leaves for each treatment was recorded. The initial aphid population was assessed before application, and the residual aphid population was assessed at 1, 3, 7, 10, and 14 days after application to calculate the control effect. Simultaneously, the efficacy was visually assessed to determine if any phytotoxicity occurred in the crops at the experimental dosage for each treatment group.

[0140] Methods for calculating drug efficacy:

[0141]

[0142] The experimental results are shown in the table below:

[0143] Table 3 Results of field efficacy trials for controlling peach aphids

[0144]

[0145] As shown in Table 3, the rational combination of Compound I and Compound II of this invention exhibits significant control effects against peach aphids: the control efficacy of the three compound treatment groups was 83.17%–88.85% 3 days after application, 88.35%–96.55% 10 days after application, and 86.50%–90.50% 14 days after application, all of which are superior to the control agents 20% Compound I suspension concentrate and 24% Compound II wettable powder. The insecticidal composition of this invention also has good rapid action, long-lasting effect, and no phytotoxicity to the target crop.

[0146] Example 4: Field efficacy test for controlling rice planthoppers

[0147] Experimental Site: The experimental site was located in Lijiatang Village, Zhongluotan Town, Baiyun District, Guangzhou City, Hubei Province. The soil texture was loam with moderate fertility and good irrigation conditions. Fertilizer and water management, crop growth, and other conditions were consistent across all experimental plots and conformed to local Good Agricultural Practices (GAP).

[0148] Experimental crop: rice.

[0149] Experimental target: rice planthopper.

[0150] Experimental design: The experiment was repeated 4 times, with a total of 24 plots, each plot having an area of ​​30m². 2 Each subdistrict is randomly grouped. Mud embankments separate the subdistricts, and protective barriers of at least 1 meter are set up around them.

[0151] Experimental method: When applying the pesticide, the Gongnong-16 backpack electric sprayer was used to spray evenly. A water layer of 2-5 cm was maintained in the field during the application and kept for 1 week after the application. During the experiment, the rice was in the late tillering stage and the rice planthopper was in the early nymphal stage.

[0152] Experimental survey: The initial insect population was investigated before pesticide application, and the number of live insects was investigated 1, 3, and 7 days after pesticide application. Ten points were investigated in each plot using the parallel line skip sampling method, with two clumps at each point. During the investigation, an enamel plate was placed under the rice plant and the planthoppers were tapped three times to make them fall into the enamel plate. The number of live insects was then counted.

[0153] Throughout the experiment, the growth, plant height, number of leaves, and leaf color of each pesticide treatment area were checked periodically and found to be no different from those of the control area. No obvious pesticide damage was found, indicating that the pesticide was safe for rice.

[0154] Methods for calculating drug efficacy:

[0155]

[0156] The field efficacy results of the pesticide are shown below:

[0157] Table 4 Results of field efficacy trials for controlling rice planthoppers

[0158]

[0159] The experimental results showed that the rational combination of Formula I and Formula III compounds had a good control effect on rice planthoppers, with outstanding rapid and sustained effects. One day after application, the control effects of the following preparations on rice planthoppers were 83.54%, 86.64%, and 92.22%, respectively: Preparation 10: 21% Formula I·Formula III wettable powder (20:1), Preparation 8: 36% Formula I·Formula III water-dispersible granules (5:1), and Preparation 2: 24% Formula I·Formula III suspension concentrate (1:5). In contrast, the control effects of the control agents, 20% Formula I suspension concentrate and 15% Formula III emulsifiable concentrate, on rice planthoppers were only 77.62% and 73.39%, respectively. Three days after application, the control efficacy of the following formulations against rice planthoppers was 88.67%, 92.50%, and 96.62%, respectively: Example 10: 21% wettable powder of Formula I and Formula III (20:1); Example 8: 36% water-dispersible granules of Formula I and Formula III (5:1); and Example 2: 24% suspension concentrate of Formula I and Formula III (1:5). In contrast, the control efficacy of the 20% suspension concentrate of Formula I and the 15% emulsifiable concentrate of Formula III against rice planthoppers was only 81.81% and 76.76%, respectively. Seven days after application, the highest control efficacy against rice planthoppers was observed in Example 2: 24% suspension concentrate of Formula I and Formula III (1:5), at 98.76%.

[0160] In summary, through indoor toxicity testing and field efficacy trials, it can be seen that the insecticidal composition of the present invention has a good control effect on aphids, rice planthoppers, etc., and is safe for the target crops. It has significant control effect and is superior to single agents in delaying the development of resistance and prolonging the duration of effect.

[0161] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. The use of an insecticidal composition for controlling pests, characterized in that, The insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is a compound of Formula II: (Formula II), wherein the mass ratio of active ingredient A to active ingredient B is 1:30 to 30:1, and the pest is alfalfa aphid.

2. The use according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:20 to 10:

1.

3. The use according to claim 1, characterized in that, Based on the total mass of the insecticidal composition, which is 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the insecticidal composition is 1 to 80 wt%.

4. The use according to claim 1, characterized in that, In addition to the active ingredient, the insecticidal composition also includes auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

5. The use according to claim 1, characterized in that, The insecticidal composition is prepared into any agriculturally permissible formulation, which may be a solid formulation or a liquid formulation.

6. The use according to claim 5, characterized in that, The solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a suspension concentrate, emulsifiable concentrate, water emulsion, microemulsion, or dispersible oil suspension.

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