A periphyton efficient culture system and water purification method

Abstract

The present application relates to the technical field of microbial culture, and discloses a high-efficiency culture system for periphyton and a water purification method, which comprises the following modules: an environment regulation module for real-time monitoring and accurate regulation of key culture environment factors, a dynamic nutrient supply module for feeding and regulating nutrient balance, a high-attachment biofilm support module for improving biofilm stability and adhesion, a low-energy consumption culture module for improving renewable energy and sewage resource technology, an ecological competition module for introducing functional strains to regulate community structure through biological competition, a self-repairing ecological feedback module for realizing community anomaly identification and automatic repair through sensing feedback, a whole-process monitoring and optimization module for supporting remote sensing and system optimization, and a low-carbon resource recovery and biological product conversion module for capturing and fixing CO2 by using periphyton. Relying on the double-core mechanism of environment regulation and ecological feedback, the system realizes accurate regulation of environmental parameters, stable maintenance of biological communities and minimization of artificial intervention, and constructs an efficient and stable artificial ecological system.

Description

Technical Field

[0001] This invention relates to the field of microbial culture and water treatment technology, specifically to a high-efficiency culture system for periwinkle and a water purification method. Background Technology

[0002] Periphyton is a type of microbial community that grows on the surface of aquatic substrates, including bacteria, attached algae, protozoa, rotifers, small crustaceans, and aquatic insect larvae. These communities typically attach to substrates such as rocks, submerged plants, and artificial carriers, playing a vital role in water purification through biofilm formation and the internal cycling of matter within the ecosystem. Periphyton is not only a key component of aquatic ecosystems but also effectively removes nitrogen, phosphorus, and other pollutants from wastewater, showing broad application prospects in wastewater resource utilization.

[0003] In recent years, significant progress has been made in the application research of algal blooms in wastewater treatment and water environment remediation. This is mainly due to their biofilm attachment and metabolic functions, achieving efficient removal of water pollutants through multiple mechanisms such as adsorption, degradation, complexation, and precipitation. Algal blooms possess advantages such as strong pollution resistance, significant nitrogen and phosphorus removal efficiency, and recyclability, thus finding initial applications in the treatment of livestock and poultry wastewater, domestic sewage, industrial wastewater, and water remediation. Currently, typical algal bloom treatment technologies developed include algal brush systems, biofilm carrier technology, and composite ecosystems, achieving good wastewater purification effects within a certain range. However, existing technologies still face many bottlenecks in practical applications, limiting their promotion and large-scale application.

[0004] First, the cultivation efficiency of periphytes is low. Traditional cultivation methods mainly rely on natural attachment and growth, which is greatly affected by environmental conditions (such as water temperature, light, and flow rate), resulting in a long cultivation cycle and difficulty in forming stable, high-density periphyte communities, thus affecting water purification efficiency. Second, the selection and optimization of attachment substrates for periphytes are insufficient. Current cultivation systems mostly use traditional carriers (such as biofilm carriers and mesh), but the attachment performance, durability, and adaptability to periphyte growth of these substrates have not been fully optimized, limiting the biofilm formation rate and stability. Third, the ecological regulation mechanism of periphytes in wastewater treatment is not yet perfect. Under the influence of environmental factors such as water flow disturbance and changes in pollutant concentration, periphyte communities are prone to dissociation and loss, affecting the stability and long-term operation of the treatment system. Furthermore, the carbon fixation function and mechanism of pericarp are unclear. The carbon fixation efficiency and condition dependence of different types of algae, photosynthetic bacteria, cyanobacteria, etc. in pericarp vary greatly. Moreover, there may be multiple mechanisms superimposed in the pericarp system, such as CO2 re-release, local circulation, or deposition fixation, which leads to unclear net carbon fixation amount and pathway, affecting the application of the carbon capture function of pericarp.

[0005] Therefore, there is an urgent need in this field to develop an efficient periwinkle culture system and water purification method. By optimizing the growth environment of periwinkles, the design of carrier structure and function, and intelligent culture control strategies, the culture efficiency and ecological function expression of periwinkles can be improved, thereby enhancing water purification and carbon sequestration capabilities. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a highly efficient cultivation system for periwinkle and a water purification method. It solves the problems of traditional systems relying primarily on manual monitoring or basic sensors, resulting in delayed data acquisition and difficulty in timely reflecting environmental changes. Furthermore, existing automated monitoring systems suffer from insufficient precision in data analysis and feedback control, failing to meet the dynamic needs of biological communities. This invention promotes the rapid enrichment of periwinkle while ensuring its long-term stability in wastewater treatment, achieving efficient, low-cost, and sustainable water purification, and providing a novel and environmentally friendly solution for wastewater resource utilization.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a high-efficiency culture system for periwinkle, characterized in that it comprises:

[0008] The environmental control module is used to monitor and precisely regulate key factors such as light, temperature, and pH in real time, creating a dynamic and controllable natural-like habitat for the periwinkle.

[0009] The dynamic nutrient supply module is used to intelligently allocate carbon, nitrogen, and phosphorus nutrients to maintain nutritional balance and improve the growth rate and metabolic efficiency of periphytes.

[0010] The high-adhesion biofilm support module enhances the adhesion of surrounding organisms and maintains the structure and stability of the biofilm by optimizing the carrier material and surface structure.

[0011] The low-energy cultivation module integrates renewable energy and wastewater resource utilization technologies to reduce cultivation costs and energy consumption, and improve system operating energy efficiency and resource recycling capabilities.

[0012] The ecological competition module introduces specific competitive or functional microorganisms to regulate community structure through biological competition, especially the natural suppression of harmful bacteria and the optimization of community structure, thereby inhibiting harmful microorganisms and protecting the surrounding biological community.

[0013] The self-healing ecological feedback module is based on sensor data and community response mechanisms to build a closed-loop feedback system that can actively identify community anomalies and trigger automatic repair strategies to maintain the ecological stability of the periphyte community.

[0014] The end-to-end monitoring and optimization module integrates remote sensing, AI algorithms, and data-driven models to achieve end-to-end parameter control and intelligent system optimization.

[0015] The low-carbon resource recycling and bioproduct conversion module is used to utilize periphytobi to absorb and fix CO2, thereby achieving carbon capture and converting it into bioenergy or environmentally friendly products.

[0016] Preferably, the environmental control module includes a sensor monitoring unit, a data acquisition and transmission unit, and an AI prediction and control unit. The sensor monitoring unit is used to monitor key parameters in the environment in real time to provide data support for subsequent control. The key parameters include pH, dissolved oxygen, and temperature. The data acquisition and transmission unit is used to collect sensor data and transmit it to the central system so that the entire culture environment can be fed back and adjusted in a timely manner. The AI ​​prediction and control unit is used to analyze historical data through AI, predict the optimal environmental conditions, and automatically adjust parameters as needed to maintain the culture environment.

[0017] Preferably, the dynamic nutrient supply module includes a nutrient sensor unit, an intelligent dispensing unit, and a slow-release nutrient unit. The nutrient sensor unit is used to monitor the nutrient demand in the cultivation environment and can provide nutrients according to the actual needs of plants or microorganisms. The intelligent dispensing unit is used to dispense the required nutrients based on sensor data and AI calculations. The slow-release nutrient unit is used to maintain a suitable nutrient concentration and reduce waste over a long period of time by slowly releasing nutrients.

[0018] Preferably, the high-adhesion biofilm support module includes a 3D-printed support unit, an electrochemical stimulation unit, and a biocompatible coating unit. The 3D-printed support unit is used to help periphytobiofilms attach and grow stably. The electrochemical stimulation unit is used to promote biofilm formation through electrochemical stimulation. The biocompatible coating unit is used to coat the support with a biocompatible material, so that the support can effectively promote the attachment and growth of the biofilm.

[0019] Preferably, the low-energy cultivation module includes a renewable energy unit, a wastewater resource utilization unit, and a low-energy cultivation system unit. The renewable energy unit is used to drive the system with renewable green energy, reducing the consumption of traditional energy. The renewable energy includes solar energy, biochemical energy, microbial microcurrent, and wind energy. Solar energy is supplied through photovoltaic conversion; biochemical energy originates from microbial metabolic energy production, including the release of electron donors; microbial microcurrent generates a low-intensity current through electron transfer between the anode and cathode of electroactive bacteria; and wind energy can be used to drive air circulation or a micro-power generation unit. The above energy sources can synergistically... The system provides basic power support for lighting, stirring, aeration, and sensor power. The wastewater resource utilization unit efficiently utilizes residual organic matter and nitrogen and phosphorus nutrients in the influent. By optimizing hydraulic retention time and biofilm attachment structure, it achieves full recovery and conversion of nutrients, reduces wastewater discharge, and simultaneously provides a sustainable nutrient source for the periclump bioculture process, constructing a resource-energy-biological coupled cycle system. The low-energy cultivation system unit combines energy management strategies and process control algorithms to minimize overall system energy consumption by adjusting operating parameters such as lighting cycle, aeration intensity, and fluid dynamics.

[0020] Preferably, the ecological competition module includes a competitive biological regulation unit, a micro-ecological environment regulation and balance unit, a biological inhibitory factor unit, and a growth promoter unit. The competitive biological regulation unit is used to establish a competitive ecological niche structure in the culture system by introducing dominant microbial communities or auxiliary organisms with specific ecological functions, including specific algae and protozoa, thereby inhibiting or replacing the growth of pathogenic or harmful microorganisms. The functional microbial communities include species with abilities such as ammonia oxidation, denitrification, organic matter degradation, and antimicrobial metabolite synthesis, such as *Lactobacillus*, *Bacillus*, *Pseudomonas*, and *Nocardia*. By controlling the introduction time, community ratio, and inoculation method, ecological regulation of the original community structure is achieved, improving system stability and biosafety. The micro-ecological environment regulation and balance unit is used to regulate environmental conditions to promote the preferential growth and continuous homeostasis of the aforementioned beneficial microorganisms. The control includes precise adjustment of parameters such as pH, temperature, dissolved oxygen concentration, nutrient salt concentration, and photoperiod to create an ecological environment conducive to the growth of functional bacteria and unfavorable to the reproduction of harmful bacteria, thus maintaining the dynamic balance of the microbial community. The biological inhibitory factor unit is used to apply naturally derived or artificially synthesized microbial inhibitory factors to inhibit target pathogenic microorganisms or competing unfavorable bacteria. The inhibitory factors include microbial antimicrobial peptides, organic acids, hydrogen peroxide, biomimetic substances, indoleacetic acid, algal active substances, or other eco-friendly biological control factors, which can act on the system through addition or induced expression. The growth promoter unit is used to add exogenous microbial growth promoters to enhance the growth rate, adhesion ability, and metabolic activity of beneficial bacteria. The growth promoters include amino acids, vitamins, indoleacetic acid, organic acids, minerals, and signaling molecules, which can produce synergistic effects with functional bacteria, further improving the efficiency and stability of microecological regulation.

[0021] Preferably, the self-healing ecological feedback module includes a health monitoring unit, an automatic repair unit, and a long-term stable regulation unit. The health monitoring unit is used to monitor the structure, activity, and growth status of the periclump biological community in real time. It continuously assesses the health status of the microbial community through environmental sensors including dissolved oxygen, conductivity, pH, and redox potential, and biosensing methods including indicator species, attached diatoms, biofluorescence, metagenomics, or qPCR data acquisition, determining whether there is pathogen infection, growth stagnation, or structural imbalance. The automatic repair unit is used to automatically activate the repair mechanism to restore system balance when microecological instability or growth disorders are detected. This repair mechanism is based on "microbial community regulation." The "control + environmental regulation" dual-path feedback strategy involves two aspects: firstly, targeted addition of functional beneficial microorganisms, including antagonistic bacteria, photosynthetic microorganisms, or community stabilizers, to replace or inhibit abnormal bacterial communities; secondly, regulation of key environmental factors to create favorable growth conditions for dominant bacterial communities, thereby promoting the spontaneous recovery of the system to a stable state. The long-term stable regulation unit is used to construct a data-driven regulation model based on historical data collected during system operation. Through AI algorithms or expert rule systems, the culture conditions are dynamically optimized. This unit can predict community response trends and adaptively adjust parameters, maintaining the stability, diversity, and functional expression of the community structure in the long term, reducing the frequency of human intervention, and improving the system's self-adaptation and self-repair capabilities.

[0022] Preferably, the full-process monitoring and optimization module includes a remote monitoring unit, an automatic optimization decision-making unit, and an anomaly detection and alarm unit. The remote monitoring unit is used to enable managers to understand the system status in a timely manner through remote real-time monitoring. The automatic optimization decision-making unit is used to automatically optimize the operation of nutrient supply and environmental regulation based on data analysis. The anomaly detection and alarm unit is used to detect abnormal situations in the system in real time and remind managers to take action through the alarm system.

[0023] Preferably, the low-carbon resource recycling and bioproduct conversion module includes a CO2 capture unit, a bioconversion unit, and a resource recycling unit. The CO2 capture unit is used to collect CO2 from the environment and extract it through physical or chemical methods. The bioconversion unit is used to absorb and convert CO2 through microorganisms or plants, converting it into biofuels or environmentally friendly products. The resource recycling unit is used to recycle and reuse the converted resources, including biofuels.

[0024] A method for efficient cultivation of periwinkle includes the following steps:

[0025] S1: The environmental control module monitors key parameters in real time through the sensor monitoring unit and transmits the data to the central system through the data acquisition and transmission unit. The AI ​​prediction and control unit automatically adjusts the environmental parameters to maintain a stable growth environment.

[0026] S2: Then, the dynamic nutrient supply module uses the nutrient sensor unit to monitor nutrient demand, the intelligent delivery unit delivers nutrients, and the slow-release nutrient unit ensures that nutrients are released slowly to reduce waste;

[0027] S3: Subsequently, a high-adhesion biofilm support module provides customized support through a 3D-printed support unit, an electrochemical stimulation unit promotes biofilm formation, and a biocompatible coating unit enhances adhesion stability.

[0028] S4: At the same time, the low-energy cultivation module provides clean energy through the renewable energy unit, the wastewater resource recovery unit recovers nutrients from the wastewater, and the low-energy cultivation system unit optimizes energy use and reduces consumption;

[0029] S5: Then, through the ecological competition module, beneficial or functional microorganisms are introduced through the competitive biological regulation unit, the microecological balance unit regulates the environment to promote the growth of functional and beneficial bacteria, and the biological inhibition factor unit inhibits pathogenic microorganisms.

[0030] S6: The self-healing ecological feedback module monitors community health in real time through the health monitoring unit, the automatic repair unit activates the repair mechanism, and the long-term stable regulation unit adjusts parameters to maintain community stability.

[0031] S7: The full-process monitoring and optimization module monitors in real time through the remote monitoring unit, the automatic optimization decision unit adjusts the system operation, and the anomaly detection and alarm unit detects and reports anomalies.

[0032] S8: The low-carbon resource recycling and bioproduct conversion module collects CO2 through the CO2 capture unit, converts CO2 into biofuel through the bioconversion unit, and recycles the converted resources for reuse.

[0033] This invention provides a highly efficient culture system for periwinkle and a water purification method. It has the following beneficial effects:

[0034] 1. Precise Environmental Control and Community Stability: This invention achieves real-time monitoring, AI-powered intelligent control, and automatic repair of the culture environment for periphyte organisms through an environmental control module and a self-healing ecological feedback module. This allows for precise control of environmental parameters, ensuring the long-term stability of the biological community. The environmental control module integrates a temperature control device, a light regulation system, a water flow distribution unit, and a dissolved oxygen optimization module. It can automatically adjust key growth factors according to the actual water conditions, promoting rapid enrichment and stable growth of periphyte organisms. The self-healing ecological feedback module collects parameters such as pH, dissolved oxygen, and nutrient concentration through a multi-dimensional sensing system and performs intelligent analysis using AI algorithms to automatically adjust culture conditions to cope with environmental fluctuations. Furthermore, this module can identify adaptive changes within the biological community and optimize interactions between microorganisms, thereby reducing biofilm shedding and loss, ensuring the long-term stability of periphyte organisms. Compared to traditional cultivation methods that rely on manual maintenance, this invention reduces human intervention, improves cultivation efficiency, and significantly enhances the adaptability of periphyte organisms to changes in the external environment.

[0035] 2. Low-Energy Operation and High-Efficiency Carbon Resource Utilization: This invention employs a low-energy cultivation module and a low-carbon resource recovery and bioproduct conversion module, achieving renewable energy supply, wastewater and CO2 recovery and utilization, and improving the system's energy efficiency. The low-energy cultivation module, combined with a photosynthetic bioreactor and intelligent aeration device, optimizes light intensity and gas exchange efficiency to reduce external energy consumption, improve biological photosynthetic efficiency, and achieve self-sufficient cultivation of *Pleurotus ostreatus*. The low-carbon resource recovery and bioproduct conversion module utilizes the powerful bio-adsorption and metabolic capabilities of *Pleurotus ostreatus* to convert pollutants such as nitrogen and phosphorus in wastewater into recyclable biological resources. By optimizing hydraulic retention time and biofilm attachment structure, it achieves full recovery and conversion of nutrients, reducing wastewater discharge and providing a sustainable nutrient source for the cultivation process of *Pleurotus ostreatus*, constructing a resource-energy-biological coupled cycle system. Simultaneously, the CO2 biological carbon fixation unit promotes photosynthetic carbon fixation by microalgae, reducing carbon emissions. Furthermore, the system can efficiently convert the biomass products of *Pleurotus ostreatus*, such as algal protein, biofuel, and high-value-added compounds, through a biomass extraction unit, achieving resource utilization. Compared to traditional high-energy-consuming water treatment systems, this invention significantly reduces operating energy consumption and improves the efficiency of carbon resource recycling, providing effective support for wastewater resource utilization and carbon neutrality goals.

[0036] 3. Ecological Competition Optimization and Enhanced Biological System Stability: This invention utilizes an ecological competition module to precisely regulate the microbial community structure, thereby enhancing the stability of the biological system and reducing the impact of invasive pathogenic microorganisms. This module employs a beneficial microbial competitive inhibition unit, introducing dominant microbial communities or auxiliary organisms (such as specific algae or protozoa) with specific ecological functions to establish a competitive ecological niche structure in the culture system. This inhibits or replaces the growth of pathogenic or harmful microorganisms. The functional microbial communities include species with capabilities for ammonia oxidation, denitrification, organic matter degradation, and antimicrobial metabolite synthesis, such as *Lactobacillus*, *Bacillus*, *Pseudomonas*, and *Nocardia*. By controlling the introduction time, community ratio, and inoculation method, ecological regulation of the original community structure is achieved, enhancing... In addition to enhancing system stability and biosafety, the microecological environment regulation and balance unit, combined with an intelligent biofilm construction device, promotes the preferential growth and sustained homeostasis of beneficial microorganisms by regulating environmental conditions. This environmental condition regulation includes precise adjustment of parameters such as pH, temperature, dissolved oxygen concentration, nutrient concentration, and photoperiod, ensuring the stable attachment of beneficial microorganisms and their functional flora on the culture carrier surface and the formation of a highly efficient biofilm. This enhances their competitive advantage against pathogens and creates an ecological environment conducive to the growth of functional flora and unfavorable to the reproduction of harmful bacteria, maintaining the dynamic balance of the microbial community. Furthermore, the precise control module for biological inhibitory factors applies naturally derived or artificially synthesized microbial inhibitory factors to suppress target pathogenic microorganisms or competing unfavorable flora. These inhibitory factors include microbial antimicrobial peptides, organic acids, hydrogen peroxide, biotin-like substances, indoleacetic acid, algal active substances, or other eco-friendly biological control factors. They can be added to the system through dosing or induced expression to further inhibit the proliferation of pathogenic microorganisms and reduce dependence on external antibiotics. Compared to traditional water treatment systems that rely on chemical agents or single microorganisms, this invention achieves microbial population optimization centered on ecological competition, significantly enhancing the long-term stability of the biological system and improving the safety and sustainability of wastewater treatment. This invention also integrates a growth promoter unit for adding exogenous microbial growth promoters to enhance the growth rate, attachment ability, and metabolic activity of beneficial bacteria. These growth promoters include amino acids, vitamins, indoleacetic acid, organic acids, minerals, and signaling molecules, which can produce synergistic effects with functional bacteria, further improving the efficiency and stability of microecological regulation.

[0037] 4. Remote Intelligent Monitoring and End-to-End Optimization: This invention constructs a remote real-time monitoring, intelligent optimization decision-making, and anomaly alarm feedback system through an end-to-end monitoring and optimization module, greatly improving system management efficiency and reducing maintenance costs. This module integrates an intelligent sensor network, which can collect key parameters such as water quality, temperature, and dissolved oxygen in real time and upload them to the intelligent cloud management system via a remote data processing terminal. The cloud management system utilizes big data analysis and AI algorithms to dynamically optimize the culture conditions of periwinkle organisms, precisely adjusting light, nutrient supply, and water flow rate to ensure the system operates in optimal condition. Furthermore, the anomaly alarm feedback mechanism can automatically send alarms and initiate emergency responses when sudden changes in water quality, degradation of the biological community, or equipment failure are detected, preventing system interruption or a decline in purification efficiency. Compared to traditional management methods relying on manual inspections, this invention achieves remote control through intelligent means, greatly reducing operation and maintenance costs and improving overall operational stability and reliability.

[0038] 5. High-Efficiency Water Purification and Wastewater Resource Utilization: This invention leverages the high pollutant removal capabilities of *Phyllostachys edulis* (a type of algae) combined with an optimized cultivation system and intelligent control strategies to achieve deep wastewater purification and promote water resource recycling. The system employs a multi-stage biofilm purification unit, fully utilizing the adsorption, complexation, and metabolic degradation effects of *Phyllostachys edulis* to efficiently remove nitrogen, phosphorus, organic pollutants, and some heavy metal ions from water. Compared to traditional water treatment processes, this invention utilizes a dynamic biofilm optimization strategy to ensure the biofilm maintains stability under high flow rates and complex water quality conditions, thereby improving purification efficiency. Furthermore, the integrated wastewater resource recovery module can extract high-value-added substances, such as bioactive compounds, algal proteins, and biofuels, from the metabolic products of *Phyllostachys edulis*, maximizing the utilization of wastewater resources. Overall, this invention not only improves water purification efficiency but also upgrades traditional wastewater treatment into a resource-recycling system, providing a new technological approach for water pollution control and green industry development. Attached Figure Description

[0039] Figure 1 This is a system architecture diagram of a high-efficiency culture system for periwinkle organisms according to the present invention;

[0040] Figure 2 This is a diagram illustrating the environmental control module architecture of a high-efficiency culture system for periwinkle organisms according to the present invention.

[0041] Figure 3 This is a diagram illustrating the relationship between the environmental control modules in a high-efficiency culture system for periwinkle organisms according to the present invention.

[0042] Figure 4 This is a diagram illustrating the dynamic nutrient supply module architecture of a high-efficiency culture system for periwinkle organisms according to the present invention.

[0043] Figure 5 This is a schematic diagram of the high-attachment biofilm support module of a periflora high-efficiency culture system of the present invention;

[0044] Figure 6 This is a diagram of the low-energy-consumption cultivation module architecture of a high-efficiency culture system for periwinkle organisms according to the present invention;

[0045] Figure 7 This is a diagram showing the low-energy-consumption cultivation module relationship architecture of a high-efficiency culture system for periwinkle organisms according to the present invention.

[0046] Figure 8 This is a diagram illustrating the ecological competition module architecture of a high-efficiency culture system for periwinkle organisms according to the present invention.

[0047] Figure 9 This is a diagram illustrating the self-healing ecological feedback module architecture of a high-efficiency culture system for periwinkle organisms according to the present invention.

[0048] Figure 10 This is a diagram of the full-process monitoring and optimization module architecture of a high-efficiency culture system for periwinkle of the present invention;

[0049] Figure 11 This is a schematic diagram of the low-carbon resource recovery and bioproduct conversion module of a high-efficiency culture system for periwinkle of the present invention.

[0050] Figure 12 This is a diagram illustrating the relationship between the self-healing ecological feedback modules of a high-efficiency culture system for periwinkle organisms according to the present invention.

[0051] Figure 13 This is a flowchart of a method for efficient cultivation of periwinkle organisms according to the present invention. Detailed Implementation

[0052] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0053] Please see the appendix Figure 1 - Appendix Figure 12 This invention provides a high-efficiency culture system for periflora, comprising:

[0054] The environmental control module is used to monitor and precisely regulate key factors such as light, temperature, and pH in real time, creating a dynamic and controllable natural-like habitat for the periwinkle.

[0055] The dynamic nutrient supply module is used to intelligently allocate carbon, nitrogen, and phosphorus nutrients to maintain nutritional balance and improve the growth rate and metabolic efficiency of periphytes.

[0056] The high-adhesion biofilm support module enhances the adhesion of surrounding organisms and maintains the structure and stability of the biofilm by optimizing the carrier material and surface structure.

[0057] The low-energy cultivation module integrates renewable energy and wastewater resource utilization technologies to reduce cultivation costs and energy consumption, and improve system operating energy efficiency and resource recycling capabilities.

[0058] The ecological competition module introduces specific competitive or functional microorganisms to regulate community structure through biological competition, especially the natural suppression of harmful bacteria and the optimization of community structure, thereby inhibiting harmful microorganisms and protecting the surrounding biological community.

[0059] The self-healing ecological feedback module is based on sensor data and community response mechanisms to build a closed-loop feedback system that can actively identify community anomalies and trigger automatic repair strategies to maintain the ecological stability of the periphyte community.

[0060] The end-to-end monitoring and optimization module integrates remote sensing, AI algorithms, and data-driven models to achieve end-to-end parameter control and intelligent system optimization.

[0061] The low-carbon resource recycling and bioproduct conversion module is used to utilize periphytobi to absorb and fix CO2, thereby achieving carbon capture and converting it into bioenergy or environmentally friendly products.

[0062] The environmental control module includes a sensor monitoring unit, a data acquisition and transmission unit, and an AI prediction and control unit. The sensor monitoring unit is used to monitor key parameters in the environment in real time, providing data support for subsequent control. Key parameters include pH, dissolved oxygen, and temperature. The data acquisition and transmission unit is used to collect sensor data and transmit it to the central system, so that the entire culture environment can be fed back and adjusted in a timely manner. The AI ​​prediction and control unit is used to analyze historical data through AI, predict the optimal environmental conditions, and automatically adjust parameters as needed to maintain the culture environment.

[0063] Specifically, the sensor monitoring unit is used to monitor key parameters in the environment in real time, such as pH, dissolved oxygen, and temperature. The pH sensor ensures a suitable acidity and alkalinity for microbial growth, the dissolved oxygen sensor maintains a sufficient oxygen supply in the wastewater, and the temperature sensor ensures stable ambient temperature. The sensors feature high precision and high response speed to adapt to the complex changes in aquaculture wastewater.

[0064] The data acquisition and transmission unit is responsible for collecting sensor data and transmitting it to the central control system. It uses wireless (Wi-Fi, LoRa) or wired (Modbus, CAN) communication methods to ensure stable and low-latency data transmission, while also having anti-interference capabilities to ensure data integrity.

[0065] The AI ​​prediction and control unit analyzes real-time and historical data to predict optimal environmental parameters and automatically adjusts variables such as pH, dissolved oxygen, and temperature. The AI ​​system employs machine learning algorithms to continuously optimize control strategies, reducing human intervention. When dissolved oxygen decreases and affects microbial metabolism, the system automatically increases oxygen supply; when the temperature deviates from the optimal range, it adjusts heating or cooling equipment to ensure stable biological growth. This effectively improves the automation level of aquaculture wastewater treatment systems, optimizes environmental parameters, increases wastewater treatment efficiency, reduces human intervention costs, and ensures long-term stable operation of the biological cultivation environment.

[0066] The dynamic nutrient supply module includes a nutrient sensor unit, an intelligent dispensing unit, and a slow-release nutrient unit. The nutrient sensor unit monitors the nutrient requirements in the cultivation environment and can provide nutrients according to the actual needs of plants or microorganisms. The intelligent dispensing unit dispenses the required nutrients based on sensor data and AI calculations. The slow-release nutrient unit maintains an appropriate nutrient concentration over a long period of time by slowly releasing nutrients, thus reducing waste.

[0067] Specifically, the nutrient sensor unit is used to monitor key nutrient parameters in the environment in real time, including the concentrations of major elements such as nitrogen, phosphorus, and potassium, as well as trace elements, to ensure that nutrient supply meets the needs of organisms. The sensor has high precision and fast response capabilities, can detect nutrient consumption in real time, and analyze biological growth trends by combining historical data to optimize nutrient supply strategies;

[0068] The intelligent nutrient delivery unit precisely controls the amount of nutrients delivered based on sensor data and AI calculation results, avoiding over- or under-delivery. Combined with machine learning algorithms, it analyzes the nutrient requirements of organisms at different growth stages, automatically adjusts the delivery concentration and frequency, achieves dynamic supply, supports automated control, and links with the environmental control module to ensure that nutrients are coordinated and matched with environmental parameters such as pH and dissolved oxygen, thereby improving growth efficiency.

[0069] The slow-release nutrient unit controls the slow release of nutrients, keeping nutrient concentration stable over a longer period of time, reducing waste and fluctuations caused by frequent additions. It can employ slow-release technologies such as smart coatings, microcapsules, or ion exchange resins to release nutrients gradually according to biological needs, improving utilization and reducing environmental pollution risks. The dynamic nutrient supply module can effectively improve the automation level of aquaculture wastewater treatment systems, accurately match nutrient requirements, optimize the cultivation environment, reduce nutrient waste, and at the same time ensure the healthy growth of plants or microorganisms, improving the overall efficiency and sustainability of the system.

[0070] The high-adhesion biofilm support module includes a 3D-printed support unit, an electrochemical stimulation unit, and a biocompatible coating unit. The 3D-printed support unit helps periphytobiota attach and grow stably, the electrochemical stimulation unit promotes biofilm formation through electrochemical stimulation, and the biocompatible coating unit coats the support with biocompatible materials, enabling the support to effectively promote biofilm attachment and growth.

[0071] Specifically, 3D-printed support units are used to construct supports with high specific surface area and porous structures to provide a suitable biofilm attachment surface. Biocompatible materials (such as polylactic acid, bioceramics or conductive polymers) are used to manufacture multi-scale structures using 3D printing technology, which improves the mechanical stability and nutrient exchange efficiency of biofilms. The geometric design of the support can optimize the growth space of microbial communities, reduce the influence of shear forces, and improve biodegradation efficiency.

[0072] Electrochemical stimulation units promote the initial attachment and thickening of biofilms by applying low-intensity electric fields or microcurrents. They utilize electrode materials (such as carbon-based or metal oxides) to form microelectric fields on the support surface, thereby regulating the charge distribution and pH value in the local environment, enhancing the adhesion ability of microorganisms, accelerating the secretion of extracellular polymers (EPS), and improving the mechanical strength and degradation activity of biofilms. Electrochemical stimulation can also be used to regulate electron donors and acceptors, optimize the degradation pathway of organic matter in wastewater, and improve pollutant removal efficiency.

[0073] The biocompatible coating unit coats the support surface with a biocompatible material to further enhance the initial adhesion and long-term stability of the biofilm. The coating material can be a natural polymer (such as chitosan or sodium alginate) or a synthetic polymer (such as modified polydopamine). Its function is to provide a hydrophilic and bioactive surface, making it easier for microorganisms to adhere and grow. The coating has anti-biofouling properties, prevents competitive attachment of non-target microorganisms, and improves the enrichment efficiency of the target biological community. The high-adhesion biofilm support module, through a combination of structural optimization, electrochemical stimulation, and biocompatible modification, significantly improves the adhesion and growth rate of the biofilm, enhances the biodegradability of the aquaculture wastewater treatment system, reduces operating and maintenance costs, and enhances the long-term stability and treatment efficiency of the system.

[0074] The low-energy cultivation module comprises a renewable energy unit, a wastewater resource recovery unit, and a low-energy cultivation system unit. The renewable energy unit drives the system with renewable green energy, reducing the consumption of traditional energy sources. Renewable energy sources include solar energy, biochemical energy, microbial microcurrents, and wind energy. Solar energy is supplied through photovoltaic conversion, biochemical energy originates from microbial metabolic energy production, including the release of electron donors, microbial microcurrents generate low-intensity currents through electron transfer between the anode and cathode of electroactive bacteria, and wind energy can be used to drive air circulation or micro-power generation units. These energy sources can synergistically provide basic power support for the system's lighting, stirring, aeration, or sensor power supply. The wastewater resource recovery unit efficiently utilizes residual organic matter and nitrogen and phosphorus nutrients in the influent. By optimizing hydraulic retention time and biofilm attachment structure, it achieves full recovery and conversion of nutrients, reduces wastewater discharge, and simultaneously provides a sustainable nutrient source for the periclump bioculture process, constructing a resource-energy-biological coupled cycle system. The low-energy cultivation system unit combines energy management strategies and process control algorithms to minimize the overall energy consumption of the system by adjusting operating parameters such as lighting cycle, aeration intensity, and fluid dynamic conditions.

[0075] Specifically, renewable energy units utilize renewable energy sources such as solar and wind power to drive systems, reducing reliance on traditional energy sources. They employ solar photovoltaic modules, wind power generation devices, and energy storage systems, combined with maximum power point tracking (MPPT) to optimize power generation efficiency, and ensure stable power supply through energy storage devices.

[0076] The wastewater resource recovery unit recycles water resources and nutrients from aquaculture wastewater, reducing wastewater discharge and providing resources for cultivation. Through technologies such as membrane filtration, biodegradation, and nutrient recovery, nutrients such as nitrogen, phosphorus, and potassium are extracted and reused in cultivation. At the same time, anaerobic fermentation is used to convert organic matter into bioenergy (such as methane or hydrogen), further reducing external energy demand.

[0077] The low-energy culture system optimizes energy use and reduces unnecessary consumption. The AI-powered intelligent control system precisely manages processes such as aeration, lighting, and temperature control. For example, it only aerates when dissolved oxygen is below a set value, uses high-efficiency LED lighting or fiber optic light guides, and combines intelligent temperature control with waste heat recovery to improve energy efficiency. Through renewable energy supply, wastewater resource recovery, and intelligent management, the biological culture system achieves low-energy operation, reduces operating costs, and improves sustainability.

[0078] The ecological competition module includes a competitive biological regulation unit, a micro-ecological environment regulation and balance unit, a biological inhibitor unit, and a growth promoter unit. The competitive biological regulation unit is used to establish a competitive ecological niche structure in the culture system by introducing dominant microbial communities or auxiliary organisms with specific ecological functions, including specific algae and protozoa, thereby inhibiting or replacing the growth of pathogenic or harmful microorganisms. Functional microbial communities include species with abilities such as ammonia oxidation, denitrification, organic matter degradation, and antimicrobial metabolite synthesis, such as *Lactobacillus*, *Bacillus*, *Pseudomonas*, and *Nocardia*. By controlling the introduction time, community ratio, and inoculation method, ecological regulation of the original community structure is achieved, improving system stability and biosafety. The micro-ecological environment regulation and balance unit is used to regulate environmental conditions to promote the preferential growth and continuous homeostasis of the aforementioned beneficial microorganisms. Environmental condition regulation includes... Precise regulation of parameters such as pH, temperature, dissolved oxygen concentration, nutrient concentration, and photoperiod creates an ecological environment conducive to the growth of functional microbial communities and unfavorable to the reproduction of harmful bacteria, maintaining the dynamic balance of the microbial community. The bioinhibition factor unit is used to apply naturally derived or artificially synthesized microbial inhibitors to inhibit target pathogenic microorganisms or competing unfavorable microbial communities. Inhibition factors include microbial antimicrobial peptides, organic acids, hydrogen peroxide, biotin, indoleacetic acid, algal active substances, or other eco-friendly biocontrol factors, which can act on the system through addition or induced expression. The growth promoter unit is used to add exogenous microbial growth promoters to enhance the growth rate, attachment ability, and metabolic activity of beneficial microbial communities. Growth promoters include amino acids, vitamins, indoleacetic acid, organic acids, minerals, and signaling molecules, which can produce synergistic effects with functional microbial communities, further improving the efficiency and stability of microecological regulation.

[0079] Specifically, the competitive biological regulation unit introduces beneficial microorganisms or other organisms to inhibit the growth of pathogenic microorganisms through competitive mechanisms. This unit selects organisms with occupancy advantage, metabolic advantage, or antagonistic effects, such as lactic acid bacteria, actinomycetes, or specific probiotic groups, to compete for nutrients, attachment sites, or produce antagonistic substances, effectively inhibiting the spread of harmful microorganisms and improving the stability of the culture system;

[0080] The microecological balance unit promotes the growth of beneficial microorganisms and enhances their competitiveness by regulating environmental conditions. Key regulatory parameters include pH, temperature, and oxygen concentration. By adjusting the pH to a suitable range for beneficial bacteria or optimizing the temperature to enhance their metabolic activity, and by combining this with an environmental control system, culture conditions can be dynamically optimized, giving beneficial microorganisms a competitive advantage.

[0081] The biological inhibitor unit selectively inhibits pathogenic microorganisms using natural or synthetic inhibitors, avoiding impact on beneficial flora. Inhibitors include bacteriocins, organic acids, and antimicrobial peptides, which can interfere with the metabolism of pathogenic microorganisms, damage their cell structure, or inhibit their growth and reproduction. Simultaneously, this unit can be combined with an intelligent delivery system to achieve precise release, improve inhibition efficiency, reduce the risk of drug resistance, and reduce the impact of pathogenic microorganisms through microbial competition, environmental optimization, and the synergistic effect of inhibitors, thereby enhancing system stability, reducing the need for external intervention, and achieving a healthy and sustainable culture environment.

[0082] The self-healing ecological feedback module includes a health monitoring unit, an automatic repair unit, and a long-term stability regulation unit. The health monitoring unit monitors the structure, activity, and growth status of the periclinal microbial community in real time. It continuously assesses the health status of the microbial community through environmental sensors (dissolved oxygen, conductivity, pH, redox potential) and biosensing methods (indicator species, attached diatoms, biofluorescence, metagenomics, or qPCR data acquisition) to determine the presence of pathogen infection, growth stagnation, or structural imbalances. The automatic repair unit automatically activates the repair mechanism to restore system balance when microecological instability or growth disorders are detected. This repair mechanism is based on "microbial community regulation + environmental regulation." The "dual-path feedback" regulation strategy involves two aspects: firstly, targeted addition of functional beneficial microorganisms, including antagonistic bacteria, photosynthetic microorganisms, or community stabilizers, to replace or suppress abnormal bacterial communities; secondly, regulation of key environmental factors to create favorable growth conditions for dominant bacterial communities, thereby promoting the spontaneous recovery of the system to a stable state. The long-term stable regulation unit is used to construct a data-driven regulation model based on historical data collected during system operation. Through AI algorithms or expert rule systems, the culture conditions are dynamically optimized. This unit can predict community response trends and adaptively adjust parameters, maintaining the stability, diversity, and functional expression of the community structure in the long term, reducing the frequency of human intervention, and improving the system's self-adaptation and self-repair capabilities.

[0083] Specifically, the health monitoring unit is used to monitor the health status of the periclump biological community in real time, detect diseases, abnormal growth or metabolic imbalances, and combine optical imaging, biosensors and AI analysis technology to dynamically monitor key parameters such as microbial community density, metabolites, pH, dissolved oxygen, etc., and identify early abnormal signals so as to activate the repair mechanism in a timely manner.

[0084] When the automatic repair unit detects community imbalance or disease, it automatically activates the repair mechanism to restore ecological balance. The repair methods include introducing beneficial microorganisms to competitively eliminate harmful microorganisms, or adjusting environmental conditions, such as optimizing pH, dissolved oxygen, and temperature, to promote the growth of beneficial bacteria. Combined with the AI ​​intelligent control system, it automatically selects the best repair plan according to different abnormal situations, improves repair efficiency, and reduces human intervention.

[0085] The long-term stable regulation unit dynamically adjusts system parameters through long-term data feedback to ensure the long-term stability of the periwinkle biological community. It uses machine learning algorithms to analyze historical data, predict environmental change trends, and automatically optimize culture parameters to avoid potential problems. It can be combined with ecological competition and low-energy culture modules to achieve multi-dimensional regulation, improve the system's long-term adaptability and sustainability. The self-healing ecological feedback module combines health monitoring, automatic repair, and long-term regulation to enhance the adaptive ability of the biological culture system, reduce the need for abnormal intervention, and ensure the stability and sustainable operation of the periwinkle biological community.

[0086] The end-to-end monitoring and optimization module includes a remote monitoring unit, an automatic optimization decision-making unit, and an anomaly detection and alarm unit. The remote monitoring unit is used to allow managers to understand the system status in a timely manner through remote real-time monitoring. The automatic optimization decision-making unit is used to automatically optimize the operation of nutrient supply and environmental regulation based on data analysis. The anomaly detection and alarm unit is used to detect abnormal situations in the system in real time and remind managers to take action through the alarm system.

[0087] Specifically, the remote monitoring unit, through a real-time remote monitoring system, helps managers understand the system's operational status at any time. Combining sensor data acquisition and cloud platform technology, it enables remote monitoring of key parameters such as nutrient concentration, environmental conditions, and energy consumption. Managers can view system operating data via PC or mobile devices and make remote adjustments or interventions as needed to ensure stable system operation.

[0088] The automatic optimization decision-making unit, based on real-time data analysis, automatically optimizes operations such as nutrient supply and environmental regulation to improve biological growth efficiency and resource utilization. Employing artificial intelligence algorithms, it analyzes the dynamic relationship between the environment and biological growth, adjusting parameters such as nutrient delivery, temperature control, and dissolved oxygen in real time to ensure the system operates at its optimal state. Through automatic optimization, it reduces human intervention, improving decision-making efficiency and system stability. The anomaly detection and alarm unit monitors abnormal situations in the system in real time, promptly identifying potential problems and issuing alarms via SMS, email, or app push notifications, ensuring that managers can take swift action to prevent widespread system failures. The end-to-end monitoring and optimization module combines real-time monitoring, intelligent optimization, and anomaly response to enhance the stability, efficiency, and safety of the biological culture system, while also improving management convenience and automation.

[0089] The low-carbon resource recycling and bioproduct conversion module includes a CO2 capture unit, a bioconversion unit, and a resource recycling unit. The CO2 capture unit is used to collect CO2 from the environment and extract it through physical or chemical methods. The bioconversion unit is used to absorb and convert CO2 through microorganisms or plants, turning it into biofuels or environmentally friendly products. The resource recycling unit is used to recycle and reuse the converted resources, including biofuels.

[0090] Specifically, the low-carbon resource recycling and bioproduct conversion module reduces carbon emissions and achieves resource reuse through CO2 recovery and bioconversion. The CO2 capture unit collects CO2 from the environment using physical or chemical methods, extracts CO2 from the gas using adsorbents or solvents, and concentrates and stores it for further conversion and utilization. The bioconversion unit absorbs CO2 through microorganisms or plants and converts it into biofuels or other environmentally friendly products. Microorganisms convert CO2 into useful biofuels (such as methane and ethanol) or bio-based chemicals through photosynthesis or anaerobic fermentation. The resource recovery unit recovers and reuses the converted biofuels and other products, reducing energy consumption and improving resource utilization efficiency. The recovered biofuels can be used for energy, and the other converted products can be used in industrial or agricultural fields, achieving recycling. Through CO2 capture, microbial conversion, and resource recovery, carbon emissions are reduced and resource utilization efficiency is improved, promoting a low-carbon economy and sustainable development.

[0091] Please see the appendix Figure 13 A highly efficient method for culturing periwinkle includes the following steps:

[0092] S1: The environmental control module monitors key parameters in real time through the sensor monitoring unit and transmits the data to the central system through the data acquisition and transmission unit. The AI ​​prediction and control unit automatically adjusts the environmental parameters to maintain a stable growth environment.

[0093] S2: Then, the dynamic nutrient supply module uses the nutrient sensor unit to monitor nutrient demand, the intelligent delivery unit delivers nutrients, and the slow-release nutrient unit ensures that nutrients are released slowly to reduce waste;

[0094] S3: Subsequently, a high-adhesion biofilm support module provides customized support through a 3D-printed support unit, an electrochemical stimulation unit promotes biofilm formation, and a biocompatible coating unit enhances adhesion stability.

[0095] S4: At the same time, the low-energy cultivation module provides clean energy through the renewable energy unit, the wastewater resource recovery unit recovers nutrients from the wastewater, and the low-energy cultivation system unit optimizes energy use and reduces consumption;

[0096] S5: Then, through the ecological competition module, beneficial microorganisms are introduced through the competitive biological regulation unit, the micro-ecological balance unit regulates the environment to promote the growth of beneficial bacteria, and the biological inhibition factor unit inhibits pathogenic microorganisms;

[0097] S6: The self-healing ecological feedback module monitors community health in real time through the health monitoring unit, the automatic repair unit activates the repair mechanism, and the long-term stable regulation unit adjusts parameters to maintain community stability.

[0098] S7: The full-process monitoring and optimization module monitors in real time through the remote monitoring unit, the automatic optimization decision unit adjusts the system operation, and the anomaly detection and alarm unit detects and reports anomalies.

[0099] S8: The low-carbon resource recycling and bioproduct conversion module collects CO2 through the CO2 capture unit, converts CO2 into biofuel through the bioconversion unit, and recycles the converted resources for reuse.

[0100] Specifically, S1: The environmental control module monitors key environmental parameters (such as pH, dissolved oxygen, temperature, etc.) in real time through the sensor monitoring unit, and transmits the data to the central control system through the data acquisition and transmission unit. Based on the collected data, the AI ​​prediction and control unit automatically adjusts the environmental parameters to ensure that the biological community within the system is in the optimal growth environment;

[0101] S2: The dynamic nutrient supply module monitors the nutrient requirements of the biological community through the nutrient sensor unit, the intelligent delivery unit delivers nutrients according to the monitoring data, and the slow-release nutrient unit ensures the slow release of nutrients, reducing waste and maintaining a continuous nutrient supply.

[0102] S3: The high-adhesion biofilm support module provides a customized support structure through 3D-printed support units to enhance biofilm adhesion, an electrochemical stimulation unit to promote biofilm formation and thickening, and a biocompatible coating unit to provide a hydrophilic surface to enhance the stability and adhesion of the biofilm.

[0103] S4: The low-energy cultivation module provides clean energy through the renewable energy unit, reducing dependence on traditional energy sources. The wastewater resource recovery unit recycles nutrients from wastewater, and the low-energy cultivation system unit optimizes the system's energy use, reducing overall energy consumption and improving system energy efficiency.

[0104] S5: The ecological competition module introduces beneficial microorganisms through the competitive biological regulation unit and uses their competitive advantage to inhibit the growth of harmful microorganisms. The microecological balance unit promotes the growth of beneficial bacteria by regulating environmental conditions (such as pH, temperature, etc.). The biological inhibition factor unit uses natural or artificially synthesized inhibitory factors to inhibit pathogenic microorganisms and maintain ecological balance.

[0105] S6: The self-healing ecological feedback module monitors the health status of the biological community in real time through the health monitoring unit. When a problem is detected, the automatic repair unit activates the repair mechanism (such as adding beneficial microorganisms or adjusting environmental conditions). The long-term stability control unit automatically adjusts system parameters through long-term data feedback to maintain the stability of the biological community.

[0106] S7: The full-process monitoring and optimization module monitors the system status in real time through the remote monitoring unit. Managers can view and adjust the system operation through remote devices. The automatic optimization decision unit automatically adjusts the system operation based on data analysis. The anomaly detection and alarm unit detects and reports anomalies in real time to ensure timely response and correction.

[0107] S8: The low-carbon resource recycling and bioproduct conversion module collects CO2 from the environment through a CO2 capture unit, and the bioconversion unit uses microorganisms or plants to convert CO2 into biofuels or other environmentally friendly products. The resource recycling unit recycles and reuses the converted resources, promoting resource recycling and reducing carbon emissions. During use, it achieves coordinated work in multiple aspects such as environmental regulation, nutrient supply, energy efficiency optimization, ecological competition, and resource recycling, improving the stability, efficiency, and sustainability of the bioculture system.

[0108] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A high-efficiency culture system for periwinkle, characterized in that, include: The environmental control module is used to monitor and precisely regulate key factors such as light, temperature, and pH in real time, creating a dynamic and controllable natural-like habitat for the periwinkle. The dynamic nutrient supply module is used to intelligently allocate carbon, nitrogen, and phosphorus nutrients to maintain nutritional balance and improve the growth rate and metabolic efficiency of periphytes. The high-adhesion biofilm support module enhances the adhesion of surrounding organisms and maintains the structure and stability of the biofilm by optimizing the carrier material and surface structure. The low-energy cultivation module integrates renewable energy and wastewater resource utilization technologies to reduce cultivation costs and energy consumption, and improve system operating energy efficiency and resource recycling capabilities. The ecological competition module introduces specific competitive or functional microorganisms to regulate community structure through biological competition, thereby inhibiting harmful microorganisms and optimizing community structure, thus protecting the surrounding biological community. The self-healing ecological feedback module is based on sensor data and community response mechanisms to build a closed-loop feedback system that can actively identify community anomalies and trigger automatic repair strategies to maintain the ecological stability of the periphyte community. The end-to-end monitoring and optimization module integrates remote sensing, AI algorithms, and data-driven models to achieve end-to-end parameter control and intelligent system optimization. The low-carbon resource recycling and bioproduct conversion module is used to utilize periphytobi to absorb and fix CO2, thereby achieving carbon capture and converting it into bioenergy or environmentally friendly products. The ecological competition module includes a competitive biological regulation unit, a micro-ecological environment regulation and balance unit, a biological inhibitory factor unit, and a growth promoter unit. The competitive biological regulation unit is used to introduce dominant microbial communities or auxiliary organisms with specific ecological functions, including specific algae and protozoa, to establish a competitive ecological niche structure in the culture system, thereby inhibiting or replacing the growth of pathogenic or harmful microorganisms. The functional microbial communities include species with abilities such as ammonia oxidation, denitrification, organic matter degradation, and antimicrobial metabolite synthesis, including *Lactobacillus*, *Bacillus*, *Pseudomonas*, and *Nocardia*. By controlling the introduction time, community ratio, and inoculation method, ecological regulation of the original community structure is achieved, improving system stability and biosafety. The micro-ecological environment regulation and balance unit is used to regulate environmental conditions to promote the preferential growth and continuous homeostasis of beneficial microorganisms. Environmental condition regulation includes precise adjustment of pH, temperature, dissolved oxygen concentration, nutrient concentration, and photoperiod parameters, thereby creating an ecological environment conducive to the growth of functional microbial communities and unfavorable to the reproduction of harmful bacteria, maintaining the dynamic balance of the microbial community. The biological inhibitor unit is used to apply naturally derived or artificially synthesized microbial inhibitors to inhibit target pathogenic microorganisms or competitive unhealthy bacterial communities. The inhibitors include microbial antimicrobial peptides, organic acids, hydrogen peroxide, biotin, algal active substances, or other eco-friendly biological control factors, which act on the system through addition or induced expression. The growth promoter unit is used to add exogenous microbial growth promoters to enhance the growth rate, adhesion ability, and metabolic activity of beneficial bacteria. The growth promoters include amino acids, vitamins, organic acids, minerals, and signaling molecules, which can produce synergistic effects with functional bacteria, further improving the efficiency and stability of microecological regulation. The self-healing ecological feedback module includes a health monitoring unit, an automatic repair unit, and a long-term stability regulation unit. The health monitoring unit monitors the structure, activity, and growth status of the periclinal microbial community in real time. It continuously assesses the health status of the microbial community using environmental sensors (including dissolved oxygen, conductivity, pH, and redox potential) and biosensing methods (including indicator species, attached diatoms, biofluorescence, metagenomics, or qPCR data acquisition) to determine the presence of pathogen infection, growth stagnation, or structural imbalances. The automatic repair unit automatically activates a repair mechanism to restore system balance when microecological instability or growth disorders are detected. This repair mechanism is based on "microbial community regulation + environmental regulation." The "environmental regulation" dual-path feedback strategy involves two aspects: firstly, targeted addition of functional beneficial microorganisms, including antagonistic bacteria, photosynthetic microorganisms, or community stabilizers, to replace or inhibit abnormal bacterial communities; secondly, regulation of key environmental factors to create favorable growth conditions for dominant bacterial communities, thereby promoting the spontaneous recovery of the system to a stable state. The long-term stable regulation unit is used to construct a data-driven regulation model based on historical data collected during system operation. Through AI algorithms or expert rule systems, the culture conditions are dynamically optimized. This unit can predict community response trends and adaptively adjust parameters, maintaining the stability, diversity, and functional expression of the community structure in the long term, reducing the frequency of human intervention, and improving the system's self-adaptation and self-repair capabilities.

2. The high-efficiency culture system for peritrichous organisms according to claim 1, characterized in that: The environmental control module includes a sensor monitoring unit, a data acquisition and transmission unit, and an AI prediction and control unit. The sensor monitoring unit is used to monitor key parameters in the environment in real time, providing data support for subsequent control. The key parameters include pH, dissolved oxygen, and temperature. The data acquisition and transmission unit is used to collect sensor data and transmit it to the central system, so that the entire culture environment can be fed back and adjusted in a timely manner. The AI ​​prediction and control unit is used to analyze historical data through AI, predict the optimal environmental conditions, and automatically adjust parameters as needed to maintain the culture environment.

3. The high-efficiency culture system for peritrichous organisms according to claim 1, characterized in that: The dynamic nutrient supply module includes a nutrient sensor unit, an intelligent dispensing unit, and a slow-release nutrient unit. The nutrient sensor unit is used to monitor the nutrient requirements in the cultivation environment and can provide nutrients according to the actual needs of plants or microorganisms. The intelligent dispensing unit is used to dispense the required nutrients based on sensor data and AI calculations. The slow-release nutrient unit is used to maintain an appropriate nutrient concentration and reduce waste by slowly releasing nutrients over a long period of time.

4. The high-efficiency culture system for peritrichous organisms according to claim 1, characterized in that: The high-adhesion biofilm support module includes a 3D-printed support unit, an electrochemical stimulation unit, and a biocompatible coating unit. The 3D-printed support unit helps periphytobiofilms attach and grow stably. The electrochemical stimulation unit promotes biofilm formation through electrochemical stimulation. The biocompatible coating unit coats the support with a biocompatible material, enabling the support to effectively promote biofilm attachment and growth.

5. The high-efficiency culture system for peritrichous organisms according to claim 1, characterized in that: The low-energy cultivation module includes a renewable energy unit, a wastewater resource recovery unit, and a low-energy cultivation system unit. The renewable energy unit is used to drive the system with renewable green energy, reducing the consumption of traditional energy sources. The renewable energy sources include solar energy, biochemical energy, microbial microcurrent, and wind energy. Solar energy is supplied through photovoltaic conversion; biochemical energy originates from microbial metabolic energy production, including the release of electron donors; microbial microcurrent generates a low-intensity current through electron transfer between the anode and cathode of electroactive bacteria; and wind energy can be used to drive air circulation or a micro-power generation unit. These energy sources can synergistically provide basic power support for the system's lighting, stirring, aeration, or sensor power supply. The wastewater resource recovery unit is used to process the influent... The system efficiently utilizes residual organic matter and nitrogen and phosphorus nutrients. By optimizing hydraulic retention time and biofilm attachment structure, it achieves full recovery and conversion of nutrients, reduces wastewater discharge, and provides a sustainable nutrient source for the cultivation of periwinkle, thus constructing a resource-energy-biological coupled cycle system. The low-energy cultivation system unit is used to combine energy management strategies and process control algorithms to minimize the overall energy consumption of the system by adjusting the operating parameters of lighting cycle, aeration intensity, and fluid dynamic conditions.

6. The high-efficiency culture system for peritrichous organisms according to claim 1, characterized in that: The full-process monitoring and optimization module includes a remote monitoring unit, an automatic optimization decision-making unit, and an anomaly detection and alarm unit. The remote monitoring unit is used to enable managers to understand the system status in a timely manner through remote real-time monitoring. The automatic optimization decision-making unit is used to automatically optimize the operation of nutrient supply and environmental regulation based on data analysis. The anomaly detection and alarm unit is used to detect abnormal situations in the system in real time and remind managers to take action through the alarm system.

7. The high-efficiency culture system for peritrichous organisms according to claim 1, characterized in that: The low-carbon resource recycling and bioproduct conversion module includes a CO2 capture unit, a bioconversion unit, and a resource recycling unit. The CO2 capture unit is used to collect CO2 from the environment and extract it through physical or chemical methods. The bioconversion unit is used to absorb and convert CO2 through microorganisms or plants, converting it into biofuels or environmentally friendly products. The resource recycling unit is used to recycle and reuse the converted resources, including biofuels.

8. A method for efficient cultivation of periclump organisms, applied to the efficient cultivation system for periclump organisms according to any one of claims 1-7, characterized in that, Includes the following steps: S1: The environmental control module monitors key parameters in real time through the sensor monitoring unit and transmits the data to the central system through the data acquisition and transmission unit. The AI ​​prediction and control unit automatically adjusts the environmental parameters to maintain a stable growth environment. S2: Then, the dynamic nutrient supply module uses the nutrient sensor unit to monitor nutrient demand, the intelligent delivery unit delivers nutrients, and the slow-release nutrient unit ensures that nutrients are released slowly to reduce waste; S3: Subsequently, a high-adhesion biofilm support module provides customized support through a 3D-printed support unit, an electrochemical stimulation unit promotes biofilm formation, and a biocompatible coating unit enhances adhesion stability. S4: At the same time, the low-energy cultivation module provides clean energy through the renewable energy unit, the wastewater resource recovery unit recovers nutrients from the wastewater, and the low-energy cultivation system unit optimizes energy use and reduces consumption; S5: Then, through the ecological competition module, beneficial or functional microorganisms are introduced through the competitive biological regulation unit, the microecological balance unit regulates the environment to promote the growth of functional and beneficial bacteria, and the biological inhibition factor unit inhibits pathogenic microorganisms. S6: The self-healing ecological feedback module monitors community health in real time through the health monitoring unit, the automatic repair unit activates the repair mechanism, and the long-term stable regulation unit adjusts parameters to maintain community stability. S7: The full-process monitoring and optimization module monitors in real time through the remote monitoring unit, the automatic optimization decision unit adjusts the system operation, and the anomaly detection and alarm unit detects and reports anomalies. S8: The low-carbon resource recycling and bioproduct conversion module collects CO2 through the CO2 capture unit, converts CO2 into biofuel through the bioconversion unit, and recycles the converted resources for reuse.

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